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Citations for World Precision Instruments :
501 - 550 of 599 citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The permeability of the differentiated epithelial layer (after 4 weeks of differentiation) was determined by measuring the TEER using an epithelial volt-ohm meter (EVOM2, World Precision Instruments, Inc.). The EVOM2 was calibrated according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Test compounds were dissolved in aCSF and locally puff-applied with a patch pipette (4–6 MΩ) at low pressure (∼1 psi) controlled by a Pneumatic Picopump (PV821, World Precision Instruments, USA) for 20 to 90s ...
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bioRxiv - Neuroscience 2023Quote: ... The virus was injected via a pulled needle of a borosilicate glass capillary (ID: 0.68mm, OD: 1.2mm; Kwik-fill, World Precision Instruments Inc., # 1B150F-4) that was angled at 50° to the slice using a stereotactic micromanipulator (SM-11 ...
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bioRxiv - Neuroscience 2024Quote: ... Local field-potential (LFP) activity was recorded using a patch pipette prepared from thin-borosilicate glass (1.5mmOD, 0.84mmID, World Precision Instruments, cat.no. 1B150F-4) pooled and fire-polished on a Flaming/Brown micropipette puller (Sutter Instrument ...
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bioRxiv - Molecular Biology 2024Quote: Glass capillary tubes with an inner diameter about 0.2 mm were prepared from thin wall glass capillaries (TW100F-4, World Precision Instruments, Sarasota, FL), using puller (PC-100 ...
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bioRxiv - Neuroscience 2023Quote: ... Triturations were carried out using two sequential bore diameters (∼300μm and ∼150μm) of fire-polished glass capillaries (World Precision Instruments; Sarasota, FL, Item No. TW100-3). A total of six triturations were used for female mice and twelve triturations for male mice ...
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bioRxiv - Neuroscience 2021Quote: ... Constant current stimuli (0.5 μA-5 mA) were applied using a high-voltage stimulus isolator (A365R, World Precision Instruments, Sarasota, FL). The evoked mono and polysynaptic IPSCs were recorded simultaneously in GCs and SGC pairs ...
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bioRxiv - Systems Biology 2021Quote: ... 1 mM beta-glycerol phosphate and 5 mM of sodium orthovanadate) using a syringe pump (Aladdin AL-1000, World precision instruments). Livers and right kidneys were quickly removed and snap frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... was injected iontophoretically (0.8—1.0 μA positive current pulses with a 7 s-on/off cycle for 5 min using A365, World Precision Instruments). The coordinates of the injection were [AP −2.9 ...
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bioRxiv - Bioengineering 2021Quote: ... The culture medium in the trachea lumen and within the chamber was refreshed via a syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and a programmable pump (flow rate ...
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bioRxiv - Bioengineering 2021Quote: ... the culture medium within the inner space of the trachea was replaced with the fresh medium once a day using a programmable syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and the removed culture medium was collected in a waste reservoir ...
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bioRxiv - Neuroscience 2023Quote: Intracortical injections were made at multiple sites (∼5-9 sites per cranial window, spaced 0.5-1 mm apart) using a microinjector (NanoFil, World Precision Instruments) fit with a 36G beveled needle (NF36BV-2 ...
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bioRxiv - Bioengineering 2023Quote: ... Drugs were infused over 5 minutes intravenously via a tail vein catheter with an infusion pump (World Precision Instruments, Sarasota, FL). Focused ultrasound was applied after 2.5 minutes of drug infusion for 2.5 minutes (250 kHz ...
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bioRxiv - Cell Biology 2020Quote: ... on to 1×105 adhered U–2–OS or U–2–OS Flp-In T-Rex cells in 2 mL media in a 3.5 cm glass bottom dish (FD35–100, WPI). 8 hr following transfection ...
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bioRxiv - Immunology 2021Quote: ... Barrier integrity of ALI cultures was monitored by measuring trans-epithelial electrical resistance (TEER, EVOM 2, World Precision Instruments). ALI cultures were validated for use in experiments based on microscopic appearance of appropriate ciliated morphology and TEER > 500 Ω*cm2 ...
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bioRxiv - Cell Biology 2021Quote: ... TEER values were determined daily beginning on day-2 using an epithelial volt/ohm meter (EVOM2, World Precision Instruments) and STX2 series chopstick electrodes as described previously (23).
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bioRxiv - Neuroscience 2024Quote: ... For all surgical procedures mice were anesthetized using 1.5-2% isoflurane and were injected using a Kopf stereotaxic and World Precision Instruments (WPI) microinjection syringe pump with a 10 mL syringe and 33g blunt needle at 50-100 nL/min ...
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bioRxiv - Neuroscience 2024Quote: ... Embryos were loaded into a 2% agarose gel mold and a pre-pulled glass needle (World Precision Instruments, Sarasota) was filled with 10 μL of the injection medium ...
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bioRxiv - Neuroscience 2024Quote: ... 600nl of vector per hemisphere was injected at 200nl/min into the lateral hypothalamic area (AP/DV/ML = ±1.6/-5.3/ ±0.9 mm) using a microinjection syringe pump (UMP3T-2 World Precision Instruments). For morphological analysis of astrocytes in the LHa viral titres were dropped ten-fold to 1011 GC/ml ...
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bioRxiv - Neuroscience 2022Quote: ... A standard micropipette injection protocol was used to make all injections into the brain or spinal cord using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Bioengineering 2020Quote: MC3T3-E1 Subclone 4 (ATCC® CRL-2593™) preosteoblastic cells were cultured on duplicate samples of tissue-cultured polystyrene (Fluorodish, World Precision Instruments) and commercially available argon-treated BC (CoArBC) ...
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bioRxiv - Biophysics 2023Quote: ... A standard micropipette injection protocol was used to make all injections into the brain using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Bioengineering 2024Quote: Molds for the device assembly were fabricated using PDMS and biopsy punches of 4 mm and 7 mm diameters (World Precision Instruments, Sarasota, FL). A large well was made in the PDMS with the 7 mm punch ...
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bioRxiv - Neuroscience 2024Quote: ... Patch pipette electrodes with a tip resistance between 4 and 6 MΩ were pulled from thin-walled borosilicate glass capillaries (World Precision Instruments, Sarasota, FL) using a P-97 Flaming/Brown Puller (Sutter Instruments ...
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bioRxiv - Neuroscience 2024Quote: ... A Hamilton syringe (Hamilton 7635-01) was filled with either PBS or C9-ASO at 50 ng/μL and attached to a microinjection pump (World Precision Instruments Micro 4) on a stereotactic arm ...
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bioRxiv - Cell Biology 2021Quote: ... was injected into each hemisphere of the M1 motor cortex using a glass pipette (tip diameter 5∼8 mm) at a rate of 100 nl/min using a syringe pump (Micro4; World Precision Instruments). The injection site was standardized among animals by using stereotaxic coordinates (ML = ±1.50 ...
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bioRxiv - Neuroscience 2022Quote: ... rats were deeply anaesthetised with urethane and lesions were made at the tip of the tetrodes using a 30 μA unipolar current for 5 – 10 s (Stimulus Isolator, World Precision Instruments). Then ...
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bioRxiv - Neuroscience 2022Quote: ... They were filled with a 1 M KCl solution using a MicroFil non-metallic syringe needle (MF 34G-5, World Precision Instruments); a few recordings were done with 3 M KCl (no particular change was noticed).
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bioRxiv - Biophysics 2023Quote: ... using a Sutter P-97 puller and heat-polished to final resistances between 1 and 5 MΩ using a MF-200 microforge (World Precision Instruments). An agar bridge (1M KCl ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated for 60 min in 5% CO2 at 37°C in suspension before adding to glass-bottom dishes (FluoroDish, FD35-100, World Precision Instruments) or 12-mm diameter coverslips (Fisherbrand 1254580 ...
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bioRxiv - Neuroscience 2022Quote: ... Neural signals were amplified (20,000 X) and filtered (300 Hz – 5 kHz) through an isolated amplifier (ISO-Dam 80, WPI, Sarasota, FL, USA). The microelectrode was manually guided into a cutaneous fascicle by delivering weak electrical pulses (0.2 ms ...
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bioRxiv - Neuroscience 2021Quote: ... 2.0-2.3 mm lateral to midline and 2.0-2.5 mm posterior to bregma) at a rate of 60 nL/min at a concentration of 2 mM using a Nanoliter 2020 system (WPI). Optogenetic silencing of V1 was achieved by the use of mice in which channelrhodopsin-2 expression was driven by cre in parvalbumin positive interneurons (Ai32 x PVcre) ...
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Red Algae-Derived Mineral Intervention to Counter Pro-inflammatory Activity in Human Colon OrganoidsbioRxiv - Molecular Biology 2022Quote: ... TEER values were determined daily with an Epithelial Volt ohm meter 2 (EVOM2) epithelial volt/ohm meter (World Precision Instruments) and STX2 series chopstick electrodes.
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bioRxiv - Neuroscience 2024Quote: ... we applied 1-s pressure pulses (0.4-0.6 psi) every 2 s for 1 min by a microinjector (PV850, World Precision Instruments). In some experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Whole-cell patch-clamp recordings were made using electrodes (2-6MΩ) pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments). They were filled with a pipette solution containing (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The compound solutions were perfused into the chamber and cells via a multichannel perfusion system (MPS-2, World Precision Instruments).
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bioRxiv - Neuroscience 2023Quote: ... and injected with a volume of 2 μL at a rate of 20 nL/s (Micro4, World Precision Instruments, USA) into the vitreous cavity (51730D ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (2–4MU) were pulled from filamental borosilicate glass capillaries (1.5 mm OD, 0.84 mm ID; World Precision Instruments) Patch pipettes were filled with internal solution containing (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... 1010 gc AAV8 in 1-2 μl was stereotactically injected with 15 nl/sec using a microinjection system (Nanoliter 2010, WPI) at (X ...
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bioRxiv - Biochemistry 2023Quote: ... 1×105 U-2 OS or U-2 OS Flp-In T-Rex cells were seeded in 35 mm glass bottom dishes (FD35-100, WPI); cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967 ...
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bioRxiv - Neuroscience 2023Quote: ... and 26-gauge needle (Hamilton) at a flow rate of 100 nl/min driven by a microsyringe pump controller (World Precision Instruments, model Micro 4). The syringe needle was left in place for 10 min and was completely withdrawn 17 min after viral delivery ...
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bioRxiv - Genetics 2023Quote: ... 0.5nl of the mixtures were directly injected into the yolk of freshly layed zygotes using a glass capillary needle [TW120-4) (Kwik-Fil, World Precision Instruments, Inc., Hertfordshire, UK)] with the help of a pedal controlled microinjector (PV820 Pneumatic PicoPump ...
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bioRxiv - Neuroscience 2023Quote: ... Recording pipettes (10-15 MΩ) were pulled from borosilicate capillary glass (BF150-86-10, Sutter Instruments, Novato, USA or 1B150F-4, World Precision Instruments, Sarasota, USA) in a horizontal pipette puller (P-1000 Micropipette puller ...
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bioRxiv - Neuroscience 2024Quote: ... plasmid was delivered using a glass pipette at a flow rate of 50 nl/min driven by a microsyringe pump controller (World Precision Instruments, model Micro 4). The syringe needle was left in place for 10 min and was completely withdrawn 17 min after viral delivery ...
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bioRxiv - Neuroscience 2021Quote: ... we filled the central barrel (with the tungsten electrode inside) with superglue using a syringe and fine flexible injection cannula (MicroFil 28 AWG, MF28G67-5, World Precision Instruments, Ltd.). We found that if we did not fill (most of ...
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bioRxiv - Neuroscience 2021Quote: ... Injectors were connected via flexible polyethylene tubing to 5-µl SGE micro-syringes mounted on a microinfusion pump (sp200IZ, World Precision Instruments, UK). A volume of 0.5µl/side of either 0.9 % sterile saline (vehicle ...
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bioRxiv - Neuroscience 2022Quote: ... cells were treated with 12-h αSN-AGs (5% v/v) for 16 h and TEER was measured using the EVOM2 apparatus with STX2 chopstick electrodes (World Precision Instruments, USA).
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bioRxiv - Neuroscience 2023Quote: ... Electrical hindpaw stimulation was applied with 1 msec 5 mA current pulses using an isolated current stimulator (A382, World Precision Instruments, Sarasota, Florida). For each stimulation condition ...
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bioRxiv - Neuroscience 2024Quote: ... Bipolar stimulation electrodes were placed on the unopened spinal cord to transmit low-intensity (5–8 V) electrical pulses generated by an isolated stimulator (A 360, WPI, Sarasota, FL, USA). This allowed antidromic activation of the M-cell axons ...
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bioRxiv - Neuroscience 2021Quote: ... +/−2.6mm ML, −4.5mm DV) using a 10μL syringe (nanofil, World Precision Instrument, USA) with NanoFil Needle (NF33BV-2, World Precision Instruments, USA) at a rate of 100nL/min (Micro4 pump ...