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Citations for World Precision Instruments :
451 - 500 of 573 citations for tert Butyl 2 chloro 5H pyrrolo 3 4 b pyridine 6 7H carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: 1.5 mm outer diameter and 1.12 mm inner diameter borosilicate glass capillaries were purchased from World Precision Instruments (WPI# TW150-4). We optimized fabrication procedures to obtain micropipettes with 2 μm diameter tips ...
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bioRxiv - Neuroscience 2022Quote: Single-unit recordings of Purkinje neurons and cerebellar nuclei neurons were performed using borosilicate glass electrodes (1B100F-4, World Precision Instruments) with 1-to 2-μm tips ...
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bioRxiv - Biophysics 2023Quote: ... using a Sutter P-97 puller and heat-polished to final resistances between 0.5 and 4 MΩ using a MF-200 microforge (World Precision Instruments). 90% series resistance (Rs ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 400 nl of AAV was injected at a rate of 80 nl/min and a depth of 0.6 mm into each of 4 injection sites with a microinjector (WPI, Nanoliter 2010) using the following coordinates (AP/ML ...
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bioRxiv - Neuroscience 2024Quote: ... Recordings were obtained using low-resistance pipettes (2.2 - 5 MΩ) pulled from filamented borosilicate glass (World Precision Instruments, Cat #1B150F-4) with a flaming/brown micropipette puller (Sutter Instruments ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... We accomplished this by having Varroa feed on pupae in which 3 μl of 1×107 DAPI microspheres in PBS buffer was injected (31 gauge needle, Micro4 microsyringe pump controller (World Precision Instruments, Sarasota, FL). A second group of pupae which served as a control were injected with PBS ...
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Clemizole and Trazodone are Effective Antiseizure Treatments in a Zebrafish Model of STXBP1 DisorderbioRxiv - Neuroscience 2022Quote: ... gap-free local field potential recordings (LFP; 60 min duration) were obtained from optic tectum using a single glass microelectrode (WPI glass #TW150 F-3) filled with 2 mM NaCl internal solution (~1 μm tip diameter ...
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bioRxiv - Neuroscience 2020Quote: ... Whole cell voltage and current-clamp recordings were performed at 32–34 °C with electrodes (3–5MΩ) pulled from borosilicate glass (World Precision Instruments, Sarasota, FL) filled with intracellular solution of the following composition (in mM) ...
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bioRxiv - Neuroscience 2021Quote: ... gap-free local field potential recordings (LFP; 15 min duration) were obtained from optic tectum using a single glass microelectrode (WPI glass #TW150 F-3); ~ 1 μm tip diameter ...
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bioRxiv - Bioengineering 2021Quote: ... New Jersey) and custom 3D-printed components was assembled around the animal to support a 3-axis micromanipulator (502600, World Precision Instruments, Sarasota, FL) and linear actuator (M-235.5DD ...
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bioRxiv - Neuroscience 2023Quote: ... A circular piece of the skull (3 mm in diameter) centered over the injection was carefully cut out using a sharp drill (Ideal micro drill, World Precision Instruments, Berlin, Germany). The dura was removed with forceps and mild vacuum suction was used to slowly remove the cortex within the craniotomy ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... at least 3 days before the day of injection and the craniotomies were covered with silicone elastomer (Kwik-Cast, World Precision Instruments, FL, USA). Before injection ...
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bioRxiv - Biophysics 2021Quote: ... The potential was measured using a Ag/AgCl mini-reference electrode (DRI-REF-2, World Precision Instruments) and a platinum working electrode (Scientific Glassblowing Service ...
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bioRxiv - Neuroscience 2020Quote: ... a tungsten bipolar electrode (2-3μm-diameter tip) with resistance of 0.5 MΩ (TST33A05KT, World Precision Instruments) was inserted into layer 2/3 of Cg1/M2 ...
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bioRxiv - Neuroscience 2021Quote: ... The caudal fin (2 mm of the end) was removed using dissecting scissors (WPI Inc., FL, USA) and placed into 1.5 ml collecting tubes ...
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bioRxiv - Neuroscience 2021Quote: ... Animals were glued onto 2% agarose pads using Nexaband® veterinary glue (World Precision Instruments, Sarasota, Florida). Stimuli were applied by moving a capillary into the buffer close to the nose of the worm ...
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bioRxiv - Bioengineering 2021Quote: ... TEER measurements were performed using an EVOM-2 and STX-100 electrodes (World Precision Instruments, Sarasota, FL). Monolayers with TEER > 1500 Ω cm2 were used for the permeability experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Viral vectors were infused using a microsyringe pump system (UMP3T-2; Nanofil with 33-Ga needles; WPI). A volume of 400 nL of the viral-containing medium was infused in each hemisphere at the target sites ...
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bioRxiv - Neuroscience 2022Quote: ... The injection was performed with a blunt 33-gauge stainless steel needle (NF33BL-2, World Precision Instruments) and a 10 µL microinjection syringe (Nanofil ...
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bioRxiv - Neuroscience 2022Quote: ... Recording glass pipettes with a tip of resistance of 1.5-2 MΩ (World Precision Instruments; Sarasota, FL) were filled with potassium-based intracellular solution (in mM) ...
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bioRxiv - Physiology 2024Quote: ... The TEER was measured using STX-2 chopstick electrodes connected to an EVOM voltohmmeter (World Precision Instruments). 1 mg/ml 4 kDa fluorescein (FITC)-dextran was added to the apical (‘luminal’ ...
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bioRxiv - Neuroscience 2021Quote: ... was assembled prior to injections into the cortex under microscopic control using a microinjection system (UMP3-1, SYS-Micro 4; World Precision Instruments). To increase optogenetic responses after one year ...
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bioRxiv - Cell Biology 2022Quote: ... IVT reticulocyte lysate was backloaded into a needle pulled from a 1 mm glass capillary tube (TW 100F-4, World Precision Instruments) using a P-87 Micropipette Puller (Sutter Instrument) ...
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bioRxiv - Neuroscience 2021Quote: ... 200 nl of 1:1 mixture of AAV5 CAG-FLEx-TCb and AAV8 CAG-FLEx-RG was injected into the PVH at a speed of 50 nl/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). For local circuit mapping (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was injected at a rate of 40 μl/min controlled via a microsyringe pump (UltraMicroPump3–4; World Precision Instruments, Sarasota, FL). Upon recovery ...
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Prelimbic proBDNF facilitates memory destabilization by regulation of neuronal function in juvenilesbioRxiv - Neuroscience 2022Quote: ... which was arrayed into a 4×8 matrix using 25-μm-diameter tungsten wires (California Fine Wires) in a 35-gauge silica tube (World Precision Instruments). A cannula was attached to a silica tube ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 250 nL AAV was dispensed at each cortical layer through a glass-capillary-attached Hamilton syringe at a rate of 40 nL/min using a UltraMicroPump-4 system (World Precision Instruments). To image UDP in hippocampus ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5-inch-long needle attached to a 5 µl Hamilton syringe was mounted to the stereotaxic frame and controlled using a Micro 4 Microsyringe Pump (World Precision Instruments) to infuse 0.5 µl AAV2/PHP.eB-hGfaABC1D-DIO-hTDP43-WT (1.2 × 1013 particles/µl) ...
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bioRxiv - Neuroscience 2022Quote: Pipettes for patch clamping were prepared freshly on the day of electrophysiology experiments from filamented glass capillaries (1B150F-4, World Precision Instruments) using a horizontal pipette puller (P1000 ...
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bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV8 hSyn-DIO-hM3Dq-mCherry or AAV5 hSyn-DIO-mCherry was bilaterally injected into the PVH at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). The coordinates for injection into the supraoptic nucleus were as follows ...
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bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV serotype 9 OTp-mCherry was bilaterally injected into the PVH and the supraoptic nucleus at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments).
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... The ion-selective microelectrodes used to measure hemolymph [K+] were fashioned from glass capillaries (TW150-4, World Precision Instruments, Sarasota, FL, USA) and pulled to a ∼ 1-3 mm tip on a Flaming-Brown micropipette puller (P-1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were plated onto either 13 mm 1.5 glass coverslips in 4-well plates or FluoroDish 35 mm dishes ready coated with poly-D-lysine (PDL) (World Precision Instruments). Glass coverslips were coated with 0.1 ug/ul PDL for 20 minutes and washed once with F12 ...
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bioRxiv - Neuroscience 2020Quote: ... cone tipped 5μl stainless steel injector (SGE Analytical Science, Australia) over 3 min using an infusion pump (UMP3 with SYS4 Micro-controller, World Precision Instruments, Inc., FL, USA). The needle was left in place for 7 min to allow for diffusion of and reduce spread up the infusion tract ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Whole cell current- and voltage-clamp recordings were performed with pipettes (3-7MΩ when filled with intracellular solution) made from borosilicate glass capillaries (World Precision Instruments, Hitchin, Herts, UK) pulled on a P-97 Flaming/Brown micropipette puller (Sutter ...
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bioRxiv - Physiology 2024Quote: ... using glass pulled microelectrodes filled with 3 M KCl (pipette resistance: 30–60 MΩ) connected to a DUO 773 electrometer (World Precision Instruments, Inc., FL, USA). For smooth muscle and endothelium recordings ...
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bioRxiv - Neuroscience 2020Quote: ... The Hamilton syringe was mounted into a motorized microinjector (Legato130, KD Scientific or UMP3T-2, WPI, Sarasota, USA) that was held by manipulator (Model 1460 ...
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bioRxiv - Biophysics 2021Quote: ... oocytes were transferred to a 2 μl media droplet in a 35 mm glass bottom FluoroDish from WPI covered with 400-500 μl of oil ...
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bioRxiv - Neuroscience 2021Quote: Recording electrodes (resistance 2-4MΩ) were pulled from borosilicate glass (Schott no. 8250; World Precision Instruments, Sarasota, Fl) using a Sutter P-97 puller (Sutter Instrument ...
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bioRxiv - Neuroscience 2021Quote: ... Recording pipettes (2-4MΩ) were pulled from borosilicate glass (Schott no. 8250; World Precision Instruments, Sarasota, FL, USA) with a Sutter P-97 puller (Sutter Instrument ...
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bioRxiv - Neuroscience 2022Quote: ... Embryos were removed and injected one at a time with 2 μL (2μg/μl) DNA containing pAAV-CAG-mRuby2 and CMV-dnKv4.2 using pulled glass micropipettes (WPI) into the lateral ventricle ...
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bioRxiv - Neuroscience 2023Quote: ... were pressure-injected into caudate nucleus (Interaural 9.75 mm) and putamen (Interaural 9.75 mm) by Hamilton syringe mounted into motorized microinjector (UMP3T-2, WPI) held by manipulator (Model 1460 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV virus was then infused via a blunt 36-gauge stainless steel needle (World Precision Instruments NF36BL-2) connected to a microliter injection system (10 µL ...
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bioRxiv - Neuroscience 2024Quote: ... the craniotomies were covered with agarose (2%) and subsequently sealed with silicone elastomer (Kwik-cast, World Precision Instruments). Recordings were performed daily for up to 3 days following the craniotomies.
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bioRxiv - Biophysics 2020Quote: ... The signal from the force transducer was amplified using Transbridge TBM4M and digitized using Lab-Trax-4 data acquisition hardware (WPI, Sarasota, FL, USA). Three phases could be distinguished on force curves recorded using LabScribe v3 (iWorks ...
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bioRxiv - Microbiology 2020Quote: The permeability of the differentiated epithelial layer (after 4 weeks of differentiation) was determined by measuring the TEER using an epithelial volt-ohm meter (EVOM2, World Precision Instruments, Inc.). The EVOM2 was calibrated according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The virus was injected via a pulled needle of a borosilicate glass capillary (ID: 0.68mm, OD: 1.2mm; Kwik-fill, World Precision Instruments Inc., # 1B150F-4) that was angled at 50° to the slice using a stereotactic micromanipulator (SM-11 ...
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bioRxiv - Neuroscience 2024Quote: ... Local field-potential (LFP) activity was recorded using a patch pipette prepared from thin-borosilicate glass (1.5mmOD, 0.84mmID, World Precision Instruments, cat.no. 1B150F-4) pooled and fire-polished on a Flaming/Brown micropipette puller (Sutter Instrument ...
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bioRxiv - Molecular Biology 2024Quote: Glass capillary tubes with an inner diameter about 0.2 mm were prepared from thin wall glass capillaries (TW100F-4, World Precision Instruments, Sarasota, FL), using puller (PC-100 ...
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bioRxiv - Neuroscience 2023Quote: ... Triturations were carried out using two sequential bore diameters (∼300μm and ∼150μm) of fire-polished glass capillaries (World Precision Instruments; Sarasota, FL, Item No. TW100-3). A total of six triturations were used for female mice and twelve triturations for male mice ...