Labshake search
Citations for World Precision Instruments :
451 - 500 of 602 citations for 3 2 5 Dioxoimidazolidin 4 yl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Clemizole and Trazodone are Effective Antiseizure Treatments in a Zebrafish Model of STXBP1 DisorderbioRxiv - Neuroscience 2022Quote: ... gap-free local field potential recordings (LFP; 60 min duration) were obtained from optic tectum using a single glass microelectrode (WPI glass #TW150 F-3) filled with 2 mM NaCl internal solution (~1 μm tip diameter ...
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bioRxiv - Neuroscience 2020Quote: ... Whole cell voltage and current-clamp recordings were performed at 32–34 °C with electrodes (3–5MΩ) pulled from borosilicate glass (World Precision Instruments, Sarasota, FL) filled with intracellular solution of the following composition (in mM) ...
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bioRxiv - Neuroscience 2021Quote: ... gap-free local field potential recordings (LFP; 15 min duration) were obtained from optic tectum using a single glass microelectrode (WPI glass #TW150 F-3); ~ 1 μm tip diameter ...
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bioRxiv - Bioengineering 2021Quote: ... New Jersey) and custom 3D-printed components was assembled around the animal to support a 3-axis micromanipulator (502600, World Precision Instruments, Sarasota, FL) and linear actuator (M-235.5DD ...
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bioRxiv - Neuroscience 2023Quote: ... A circular piece of the skull (3 mm in diameter) centered over the injection was carefully cut out using a sharp drill (Ideal micro drill, World Precision Instruments, Berlin, Germany). The dura was removed with forceps and mild vacuum suction was used to slowly remove the cortex within the craniotomy ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... at least 3 days before the day of injection and the craniotomies were covered with silicone elastomer (Kwik-Cast, World Precision Instruments, FL, USA). Before injection ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (3-6 MΩ) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Biophysics 2021Quote: ... The potential was measured using a Ag/AgCl mini-reference electrode (DRI-REF-2, World Precision Instruments) and a platinum working electrode (Scientific Glassblowing Service ...
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bioRxiv - Neuroscience 2020Quote: ... a tungsten bipolar electrode (2-3μm-diameter tip) with resistance of 0.5 MΩ (TST33A05KT, World Precision Instruments) was inserted into layer 2/3 of Cg1/M2 ...
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bioRxiv - Neuroscience 2021Quote: ... The caudal fin (2 mm of the end) was removed using dissecting scissors (WPI Inc., FL, USA) and placed into 1.5 ml collecting tubes ...
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bioRxiv - Neuroscience 2021Quote: ... Animals were glued onto 2% agarose pads using Nexaband® veterinary glue (World Precision Instruments, Sarasota, Florida). Stimuli were applied by moving a capillary into the buffer close to the nose of the worm ...
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bioRxiv - Bioengineering 2021Quote: ... TEER measurements were performed using an EVOM-2 and STX-100 electrodes (World Precision Instruments, Sarasota, FL). Monolayers with TEER > 1500 Ω cm2 were used for the permeability experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Viral vectors were infused using a microsyringe pump system (UMP3T-2; Nanofil with 33-Ga needles; WPI). A volume of 400 nL of the viral-containing medium was infused in each hemisphere at the target sites ...
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bioRxiv - Neuroscience 2022Quote: ... The injection was performed with a blunt 33-gauge stainless steel needle (NF33BL-2, World Precision Instruments) and a 10 µL microinjection syringe (Nanofil ...
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bioRxiv - Neuroscience 2022Quote: ... Recording glass pipettes with a tip of resistance of 1.5-2 MΩ (World Precision Instruments; Sarasota, FL) were filled with potassium-based intracellular solution (in mM) ...
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bioRxiv - Physiology 2024Quote: ... The TEER was measured using STX-2 chopstick electrodes connected to an EVOM voltohmmeter (World Precision Instruments). 1 mg/ml 4 kDa fluorescein (FITC)-dextran was added to the apical (‘luminal’ ...
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bioRxiv - Neuroscience 2021Quote: ... was assembled prior to injections into the cortex under microscopic control using a microinjection system (UMP3-1, SYS-Micro 4; World Precision Instruments). To increase optogenetic responses after one year ...
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bioRxiv - Cell Biology 2022Quote: ... IVT reticulocyte lysate was backloaded into a needle pulled from a 1 mm glass capillary tube (TW 100F-4, World Precision Instruments) using a P-87 Micropipette Puller (Sutter Instrument) ...
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bioRxiv - Neuroscience 2021Quote: ... 200 nl of 1:1 mixture of AAV5 CAG-FLEx-TCb and AAV8 CAG-FLEx-RG was injected into the PVH at a speed of 50 nl/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). For local circuit mapping (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was injected at a rate of 40 μl/min controlled via a microsyringe pump (UltraMicroPump3–4; World Precision Instruments, Sarasota, FL). Upon recovery ...
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Prelimbic proBDNF facilitates memory destabilization by regulation of neuronal function in juvenilesbioRxiv - Neuroscience 2022Quote: ... which was arrayed into a 4×8 matrix using 25-μm-diameter tungsten wires (California Fine Wires) in a 35-gauge silica tube (World Precision Instruments). A cannula was attached to a silica tube ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 250 nL AAV was dispensed at each cortical layer through a glass-capillary-attached Hamilton syringe at a rate of 40 nL/min using a UltraMicroPump-4 system (World Precision Instruments). To image UDP in hippocampus ...
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bioRxiv - Neuroscience 2022Quote: Pipettes for patch clamping were prepared freshly on the day of electrophysiology experiments from filamented glass capillaries (1B150F-4, World Precision Instruments) using a horizontal pipette puller (P1000 ...
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bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV8 hSyn-DIO-hM3Dq-mCherry or AAV5 hSyn-DIO-mCherry was bilaterally injected into the PVH at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). The coordinates for injection into the supraoptic nucleus were as follows ...
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bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV serotype 9 OTp-mCherry was bilaterally injected into the PVH and the supraoptic nucleus at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments).
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... The ion-selective microelectrodes used to measure hemolymph [K+] were fashioned from glass capillaries (TW150-4, World Precision Instruments, Sarasota, FL, USA) and pulled to a ∼ 1-3 mm tip on a Flaming-Brown micropipette puller (P-1000 ...
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bioRxiv - Neuroscience 2021Quote: ... mice were anesthetized with isoflurane (induction, 5%; maintenance, 0.5 to 1.5%) and placed into a stereotaxic frame (World Precision Instruments). The body temperature was maintained at 36° C using a heating pad ...
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bioRxiv - Neuroscience 2020Quote: ... Viral injections were performed using a 32-gauge syringe (Hamilton Syringes Neuros, #65458) at 5-7 nl/min rate using a microsyringe pump (micro4, WPI). For multiple injections ...
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bioRxiv - Biophysics 2020Quote: ... After punching a fluid port through the 5 mm thick Sylgard using 0.75 mm diameter biopsy punches (World Precision Instruments) the PDMS chip was bonded onto glass substrates using an 0.3 mbar ...
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bioRxiv - Neuroscience 2022Quote: ... Pups were anesthetized under hypothermia and 5 pulses of 69 nL were injected into the V1 with a nanoliter injector (WPI). Four days later ...
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bioRxiv - Biophysics 2021Quote: ... The tip of the pipette was cut to an opening diameter between 1~ 5 μm and bent to ~40° using a microforge (DMF1000, WPI).
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bioRxiv - Neuroscience 2023Quote: ... Cells expressing YFP and with a soma diameter <25 µm were targeted for whole cell recording using pipettes (∼5 MΩ resistance) pulled from borosilicate glass (WPI).
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bioRxiv - Neuroscience 2024Quote: ... transfected slices were then transferred to the microscope chamber perfused with carbogen (95% O2 / 5% CO2)-bubbled recording aCSF maintained at approximately 30 - 34 °C by an in-line solution heater (WPI). The aCSF contained (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV viral vectors mixed with FastGreen for visualization (ratio 1:5) were injected using a PicoSpritzer (Cat. num. PV820, WPI) through pulled (P-30 puller ...
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bioRxiv - Neuroscience 2022Quote: ... electrolytic lesions were obtained by passing positive and negative current (20-25 uA) on each electrode contact for 5 s with a stimulus isolator (A365RC, WPI) while the animal was under isofluorane-induced anesthesia ...
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bioRxiv - Neuroscience 2024Quote: ... square craniotomy (1-1-5 mm diameter) was made and covered with a coverslip (World Precision Instruments, coverslip No. 1) fitting the size of the bone removed ...
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bioRxiv - Neuroscience 2024Quote: ... was slowly inserted into the brain and 0.5 to 1 µl of viral particles were injected into the hippocampus using a microinjection pump (50 nl/min, WPI). The injection needle was left in place for about 3 minutes and then slowly retracted ...
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bioRxiv - Bioengineering 2024Quote: ... The skin incision was closed using absorbable (5-0 PDS) subcuticular sutures and topical tissue adhesive (GLUture, World Precision Instruments) was applied to the incision ...
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bioRxiv - Neuroscience 2020Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2022Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2021Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2020Quote: ... cone tipped 5μl stainless steel injector (SGE Analytical Science, Australia) over 3 min using an infusion pump (UMP3 with SYS4 Micro-controller, World Precision Instruments, Inc., FL, USA). The needle was left in place for 7 min to allow for diffusion of and reduce spread up the infusion tract ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Whole cell current- and voltage-clamp recordings were performed with pipettes (3-7MΩ when filled with intracellular solution) made from borosilicate glass capillaries (World Precision Instruments, Hitchin, Herts, UK) pulled on a P-97 Flaming/Brown micropipette puller (Sutter ...
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bioRxiv - Physiology 2024Quote: ... using glass pulled microelectrodes filled with 3 M KCl (pipette resistance: 30–60 MΩ) connected to a DUO 773 electrometer (World Precision Instruments, Inc., FL, USA). For smooth muscle and endothelium recordings ...
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bioRxiv - Neuroscience 2020Quote: ... The Hamilton syringe was mounted into a motorized microinjector (Legato130, KD Scientific or UMP3T-2, WPI, Sarasota, USA) that was held by manipulator (Model 1460 ...
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bioRxiv - Biophysics 2021Quote: ... oocytes were transferred to a 2 μl media droplet in a 35 mm glass bottom FluoroDish from WPI covered with 400-500 μl of oil ...
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bioRxiv - Neuroscience 2021Quote: Recording electrodes (resistance 2-4MΩ) were pulled from borosilicate glass (Schott no. 8250; World Precision Instruments, Sarasota, Fl) using a Sutter P-97 puller (Sutter Instrument ...
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bioRxiv - Neuroscience 2021Quote: ... Recording pipettes (2-4MΩ) were pulled from borosilicate glass (Schott no. 8250; World Precision Instruments, Sarasota, FL, USA) with a Sutter P-97 puller (Sutter Instrument ...
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bioRxiv - Neuroscience 2022Quote: ... Embryos were removed and injected one at a time with 2 μL (2μg/μl) DNA containing pAAV-CAG-mRuby2 and CMV-dnKv4.2 using pulled glass micropipettes (WPI) into the lateral ventricle ...
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bioRxiv - Neuroscience 2023Quote: ... were pressure-injected into caudate nucleus (Interaural 9.75 mm) and putamen (Interaural 9.75 mm) by Hamilton syringe mounted into motorized microinjector (UMP3T-2, WPI) held by manipulator (Model 1460 ...