Labshake search
Citations for World Precision Instruments :
451 - 500 of 599 citations for 2' 4' Dimethyl 3 3 5 dimethylphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... A total of 400 nl of AAV was injected at a rate of 80 nl/min and a depth of 0.6 mm into each of 4 injection sites with a microinjector (WPI, Nanoliter 2010) using the following coordinates (AP/ML ...
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bioRxiv - Neuroscience 2024Quote: ... was puffed locally at the surface of the slice near the recorded cell through a micropipette (4-6 MΩ tip resistance; 1 sec puff duration) with a pneumatic drug ejection system [PicoPump PV 820 (WPI, Germany)] in presence or absence of strychnine (10 µM) ...
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bioRxiv - Neuroscience 2024Quote: ... Muscle cells were subsequently patched using fire-polished borosilicate pipettes with a resistance of 4-6 MΩ (World Precision Instruments, USA). We recorded membrane currents and potentials in a whole-cell configuration using a Digidata 1440A and a MultiClamp 700A amplifier ...
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bioRxiv - Biophysics 2022Quote: ... A micropipette was filled with fluorescently labeled protein using a MicroFil flexible needle (MF34G-5, World Precision Instruments, UK). The micropipettes were introduced in each side of the chamber and placed in the middle using micromanipulators ...
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bioRxiv - Neuroscience 2020Quote: ... the optic nerve adjacent to the globe was exposed by blunt dissection and crushed under visual guidance for 10 secs using 5/45 45°-angled jeweler’s forceps (WPI). The crush site was visually verified by two investigators ...
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bioRxiv - Neuroscience 2021Quote: ... Patch-clamp pipettes (5 to 8 MΩ) were pulled from capillary glass (1.5 mm diameter; World Precision Instruments, USA) using a P-87 pipette puller (Sutter Instrument Co. ...
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bioRxiv - Biophysics 2024Quote: Micropipettes with inner diameter of 5–10 μm were prepared from glass capillaries (World Precision Instruments, Sarasota, FL, USA) using of a micropipette puller (Sutter Instruments USA ...
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bioRxiv - Genetics 2023Quote: ... We performed the vector or vehicle (PBS with 0.001% PF68) delivery with an injection rate of 5 nL/s controlled by Nanoliter Microinjection Controller (WPI). The RWM niche was sealed with a small plug of muscle quickly after the injection ...
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bioRxiv - Neuroscience 2023Quote: ... Fire-polished recording pipets with resistances ranging from 5-9 MΩ were pulled from borosilicate glass (World Precision Instruments). The patch pipette was filled with 120mM KCl ...
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bioRxiv - Neuroscience 2024Quote: ... electrolytic lesions of 10 µA were applied for 5 s using a high current stimulus isolator (World Precision Instruments) to identify electrode bundle tip location prior to PFA perfusion ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... each larvae was transected at the level of the cloaca using a 5 mm microsurgical blade (World Precision Instruments). Immediately following transection ...
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bioRxiv - Biophysics 2021Quote: ... The potential was measured using a Ag/AgCl mini-reference electrode (DRI-REF-2, World Precision Instruments) and a platinum working electrode (Scientific Glassblowing Service ...
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bioRxiv - Neuroscience 2020Quote: ... a tungsten bipolar electrode (2-3μm-diameter tip) with resistance of 0.5 MΩ (TST33A05KT, World Precision Instruments) was inserted into layer 2/3 of Cg1/M2 ...
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bioRxiv - Neuroscience 2021Quote: ... The caudal fin (2 mm of the end) was removed using dissecting scissors (WPI Inc., FL, USA) and placed into 1.5 ml collecting tubes ...
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bioRxiv - Neuroscience 2021Quote: ... Animals were glued onto 2% agarose pads using Nexaband® veterinary glue (World Precision Instruments, Sarasota, Florida). Stimuli were applied by moving a capillary into the buffer close to the nose of the worm ...
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bioRxiv - Bioengineering 2021Quote: ... TEER measurements were performed using an EVOM-2 and STX-100 electrodes (World Precision Instruments, Sarasota, FL). Monolayers with TEER > 1500 Ω cm2 were used for the permeability experiments ...
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bioRxiv - Neuroscience 2022Quote: ... Recording glass pipettes with a tip of resistance of 1.5-2 MΩ (World Precision Instruments; Sarasota, FL) were filled with potassium-based intracellular solution (in mM) ...
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bioRxiv - Neuroscience 2022Quote: ... The injection was performed with a blunt 33-gauge stainless steel needle (NF33BL-2, World Precision Instruments) and a 10 µL microinjection syringe (Nanofil ...
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bioRxiv - Neuroscience 2023Quote: ... Viral vectors were infused using a microsyringe pump system (UMP3T-2; Nanofil with 33-Ga needles; WPI). A volume of 400 nL of the viral-containing medium was infused in each hemisphere at the target sites ...
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bioRxiv - Physiology 2024Quote: ... The TEER was measured using STX-2 chopstick electrodes connected to an EVOM voltohmmeter (World Precision Instruments). 1 mg/ml 4 kDa fluorescein (FITC)-dextran was added to the apical (‘luminal’ ...
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bioRxiv - Neuroscience 2021Quote: ... was assembled prior to injections into the cortex under microscopic control using a microinjection system (UMP3-1, SYS-Micro 4; World Precision Instruments). To increase optogenetic responses after one year ...
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bioRxiv - Cell Biology 2022Quote: ... IVT reticulocyte lysate was backloaded into a needle pulled from a 1 mm glass capillary tube (TW 100F-4, World Precision Instruments) using a P-87 Micropipette Puller (Sutter Instrument) ...
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bioRxiv - Neuroscience 2021Quote: ... 200 nl of 1:1 mixture of AAV5 CAG-FLEx-TCb and AAV8 CAG-FLEx-RG was injected into the PVH at a speed of 50 nl/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). For local circuit mapping (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was injected at a rate of 40 μl/min controlled via a microsyringe pump (UltraMicroPump3–4; World Precision Instruments, Sarasota, FL). Upon recovery ...
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Prelimbic proBDNF facilitates memory destabilization by regulation of neuronal function in juvenilesbioRxiv - Neuroscience 2022Quote: ... which was arrayed into a 4×8 matrix using 25-μm-diameter tungsten wires (California Fine Wires) in a 35-gauge silica tube (World Precision Instruments). A cannula was attached to a silica tube ...
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bioRxiv - Neuroscience 2022Quote: Pipettes for patch clamping were prepared freshly on the day of electrophysiology experiments from filamented glass capillaries (1B150F-4, World Precision Instruments) using a horizontal pipette puller (P1000 ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 250 nL AAV was dispensed at each cortical layer through a glass-capillary-attached Hamilton syringe at a rate of 40 nL/min using a UltraMicroPump-4 system (World Precision Instruments). To image UDP in hippocampus ...
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bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV serotype 9 OTp-mCherry was bilaterally injected into the PVH and the supraoptic nucleus at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments).
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bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV8 hSyn-DIO-hM3Dq-mCherry or AAV5 hSyn-DIO-mCherry was bilaterally injected into the PVH at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). The coordinates for injection into the supraoptic nucleus were as follows ...
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... The ion-selective microelectrodes used to measure hemolymph [K+] were fashioned from glass capillaries (TW150-4, World Precision Instruments, Sarasota, FL, USA) and pulled to a ∼ 1-3 mm tip on a Flaming-Brown micropipette puller (P-1000 ...
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bioRxiv - Neuroscience 2021Quote: ... mice were anesthetized with isoflurane (induction, 5%; maintenance, 0.5 to 1.5%) and placed into a stereotaxic frame (World Precision Instruments). The body temperature was maintained at 36° C using a heating pad ...
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bioRxiv - Neuroscience 2020Quote: ... Viral injections were performed using a 32-gauge syringe (Hamilton Syringes Neuros, #65458) at 5-7 nl/min rate using a microsyringe pump (micro4, WPI). For multiple injections ...
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bioRxiv - Biophysics 2020Quote: ... After punching a fluid port through the 5 mm thick Sylgard using 0.75 mm diameter biopsy punches (World Precision Instruments) the PDMS chip was bonded onto glass substrates using an 0.3 mbar ...
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bioRxiv - Neuroscience 2022Quote: ... Pups were anesthetized under hypothermia and 5 pulses of 69 nL were injected into the V1 with a nanoliter injector (WPI). Four days later ...
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bioRxiv - Biophysics 2021Quote: ... The tip of the pipette was cut to an opening diameter between 1~ 5 μm and bent to ~40° using a microforge (DMF1000, WPI).
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bioRxiv - Neuroscience 2022Quote: ... electrolytic lesions were obtained by passing positive and negative current (20-25 uA) on each electrode contact for 5 s with a stimulus isolator (A365RC, WPI) while the animal was under isofluorane-induced anesthesia ...
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bioRxiv - Neuroscience 2024Quote: ... transfected slices were then transferred to the microscope chamber perfused with carbogen (95% O2 / 5% CO2)-bubbled recording aCSF maintained at approximately 30 - 34 °C by an in-line solution heater (WPI). The aCSF contained (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV viral vectors mixed with FastGreen for visualization (ratio 1:5) were injected using a PicoSpritzer (Cat. num. PV820, WPI) through pulled (P-30 puller ...
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bioRxiv - Neuroscience 2023Quote: ... Cells expressing YFP and with a soma diameter <25 µm were targeted for whole cell recording using pipettes (∼5 MΩ resistance) pulled from borosilicate glass (WPI).
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bioRxiv - Neuroscience 2024Quote: ... square craniotomy (1-1-5 mm diameter) was made and covered with a coverslip (World Precision Instruments, coverslip No. 1) fitting the size of the bone removed ...
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bioRxiv - Neuroscience 2024Quote: ... was slowly inserted into the brain and 0.5 to 1 µl of viral particles were injected into the hippocampus using a microinjection pump (50 nl/min, WPI). The injection needle was left in place for about 3 minutes and then slowly retracted ...
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bioRxiv - Bioengineering 2024Quote: ... The skin incision was closed using absorbable (5-0 PDS) subcuticular sutures and topical tissue adhesive (GLUture, World Precision Instruments) was applied to the incision ...
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bioRxiv - Neuroscience 2020Quote: ... The Hamilton syringe was mounted into a motorized microinjector (Legato130, KD Scientific or UMP3T-2, WPI, Sarasota, USA) that was held by manipulator (Model 1460 ...
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bioRxiv - Biophysics 2021Quote: ... oocytes were transferred to a 2 μl media droplet in a 35 mm glass bottom FluoroDish from WPI covered with 400-500 μl of oil ...
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bioRxiv - Neuroscience 2021Quote: Recording electrodes (resistance 2-4MΩ) were pulled from borosilicate glass (Schott no. 8250; World Precision Instruments, Sarasota, Fl) using a Sutter P-97 puller (Sutter Instrument ...
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bioRxiv - Neuroscience 2021Quote: ... Recording pipettes (2-4MΩ) were pulled from borosilicate glass (Schott no. 8250; World Precision Instruments, Sarasota, FL, USA) with a Sutter P-97 puller (Sutter Instrument ...
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bioRxiv - Neuroscience 2022Quote: ... Embryos were removed and injected one at a time with 2 μL (2μg/μl) DNA containing pAAV-CAG-mRuby2 and CMV-dnKv4.2 using pulled glass micropipettes (WPI) into the lateral ventricle ...
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bioRxiv - Neuroscience 2023Quote: ... AAV virus was then infused via a blunt 36-gauge stainless steel needle (World Precision Instruments NF36BL-2) connected to a microliter injection system (10 µL ...
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bioRxiv - Neuroscience 2023Quote: ... were pressure-injected into caudate nucleus (Interaural 9.75 mm) and putamen (Interaural 9.75 mm) by Hamilton syringe mounted into motorized microinjector (UMP3T-2, WPI) held by manipulator (Model 1460 ...
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bioRxiv - Neuroscience 2024Quote: ... the craniotomies were covered with agarose (2%) and subsequently sealed with silicone elastomer (Kwik-cast, World Precision Instruments). Recordings were performed daily for up to 3 days following the craniotomies.