Labshake search
Citations for Abclonal Science :
1 - 50 of 52 citations for hsa mir 146a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... RT-PCR was performed using SYBR Green Real-time PCR Master Mix (RK21203, ABclonal Technology) under the following reaction conditions (35 cycles) ...
-
bioRxiv - Immunology 2023Quote: ... Real-time PCR was performed with 2×Universal SYBR Green kit (ABclonal, CHN). The fluorescence signal was collected by CFX96 PCR instrument (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... Real-time quantitative PCR was performed using SYBR Green Mix (Abclonal, #RK21203) following manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2021Quote: ... Real-time quantitative PCR was performed using SYBR Green Fast qPCR Mix (ABclonal, RM21203) and a BioRad CFX96 machine ...
-
bioRxiv - Immunology 2023Quote: ... Real-time quantitative PCR (Q-PCR) was performed in triplicate using 2 × Universal SYBR Green Fast qPCR Mix (ABclonal) by QuantStudio™ 7 Flex Real-Time PCR System ...
-
bioRxiv - Microbiology 2020Quote: ... and the relative quantitative abundance of selected genes was assayed by real-time PCR using the SYBR Premix Ex Taq (ABclonal, WuHan, China) with an ABI 7500 sequence detection system (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1 μg of RNA was transcribed into complementary DNA (cDNA) using the ABScript III RT-PCR kit (ABclonal Technology, RK20429). The qPCR was performed using an SYBR Green reaction mix (ABclonal Technology ...
-
bioRxiv - Immunology 2023Quote: Total RNA of cells or tumor tissues was isolated with TRIzol (10606ES60; Yeasen) and cDNA was generated with an RT reagent kit (RK20428; ABclonal). Quantitative PCR was carried out with 2× SYBR Green Fast qPCR Mix (RK21203 ...
-
bioRxiv - Molecular Biology 2024Quote: ... was used for reverse transcription in a 20 μL reaction using the ABScript III RT Master Mix for qPCR with a gDNA Remover kit (Abclonal). In each reaction ...
-
bioRxiv - Pathology 2022Quote: ... with the PCR Index Primers in Dual DNA Adapter 96 Kit for Illumina (ABclonal, Wuhan, China). Each sequencing was performed on an Illumina NovaSeq 6000 platform up to 6 Gb data with paired-end 150 nt reads (PE150 ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by detection via western blotting with the GST antibody (ABclonal).
-
bioRxiv - Genomics 2022Quote: ... reverse transcription was performed by ABScript III RT Master Mix (ABclonal).
-
bioRxiv - Neuroscience 2022Quote: ... then cDNA was synthesized using ABScript III RT Master Mix (RM21452, ABclonal Technology). RT-PCR was performed using SYBR Green Real-time PCR Master Mix (RK21203 ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by species-specific peroxidase conjugated secondary antibodies and chemiluminescent detection (Tanon, Abclonal Technologies).
-
bioRxiv - Molecular Biology 2022Quote: ... The resultant cDNA was analyzed by PCR with PowerPol 2 × PCR Mix (Abclonal, RK20719) using primers listed in supplementary table 5 ...
-
bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed using 2× Universal SYBR Green Fast qPCR Mix (RK21203, ABclonal, China) in a CFX384 Touch Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Immunology 2023Quote: ... The extracted RNA was then reverse transcribed into cDNA using ABScript II RT Mix (ABclonal, CHN). Real-time PCR was performed with 2×Universal SYBR Green kit (ABclonal ...
-
bioRxiv - Cell Biology 2023Quote: ... A donor fragment was generated from the plasmid template by PCR using Powerpol 2X PCR mix (ABclonal) (primer sequences can be found in Table 1) ...
-
bioRxiv - Developmental Biology 2022Quote: ... and reverse-transcribed into cDNA with ABScript II-RT Mix for qPCR with gDNA Remover (ABclonal, RK20403) for gene cloning ...
-
bioRxiv - Physiology 2023Quote: ... RT-qPCRs were conducted in triplicates using 1X Sybr Green Fast qPCR mix (#RK21200, ABclonal, Woburn, MA) and 100nM primers at a CFX96 qPCR machine (Bio-Rad ...
-
bioRxiv - Physiology 2022Quote: ... RT-qPCRs were conducted in three replicates using 1X Sybr Green Fast qPCR mix (#RK21200, ABclonal, Woburn, MA) and 100nM primers at a CFX96 qPCR machine (Bio-Rad ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized from 0.5 ug/sample of total RNA with the ABScript II RT Mix (ABclonal RM21452) according to the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2019Quote: ... followed by the species-appropriate peroxidase-conjugated antibodies (Thermo Fischer Scientific) and chemiluminescent detection (Tanon™ High-sig ECL Western Blotting Substrate, Abclonal). All western blotting experiments were performed using samples from three independent experiments ...
-
bioRxiv - Microbiology 2022Quote: ... except cells were permeabilized with 100% methanol for 5 min at RT instead of Triton X-100 and the following antibodies and stains were used: rabbit anti-LC3B (ABclonal #A7198), mouse anti-Rickettsia 14-13 ...
-
bioRxiv - Cell Biology 2023Quote: ... Equal amounts of RNA templates were reverse transcribed into cDNAs using ABScript III RT Master Mix for qPCR (ABclonal, Cat. RK20429). The resulting cDNAs were utilized in qPCR reactions employing Universal SYBR Green Fast qPCR Mix (ABclonal ...
-
bioRxiv - Synthetic Biology 2023Quote: ... qRT-PCR was performed using Universal SYBR Green Master Mix (Abclonal) and gene specific primers (Supplementary Table) ...
-
bioRxiv - Immunology 2023Quote: ... Quantitative PCR was carried out with 2× SYBR Green Fast qPCR Mix (RK21203; ABclonal) to assess target mRNA expression ...
-
bioRxiv - Neuroscience 2022Quote: ... Add 100 μL Working Biotin Conjugate Antibody (Rat IL-6 ELISA Kit, RK00020; Rat IL-1β ELISA Kit, RK00009; Rat IL-10 ELISA Kit, RK00050, Abclonal, China) in each well ...
-
bioRxiv - Developmental Biology 2023Quote: ... Libraries were prepared with the KAPA mRNA HyperPrep Kit (BioMek) or with the RNA library prep kit (ABClonal) following the manufacturers’ protocols ...
-
bioRxiv - Microbiology 2023Quote: ... Mouse LH ELISA Kit (Abclonal Cat. # RK02986). All assays using normalized protein amounts were performed according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Mouse INHB ELISA kit (Abclonal Cat. # RK02949), Mouse LH ELISA Kit (Abclonal Cat ...
-
bioRxiv - Microbiology 2023Quote: ... Mouse FSH ELISA Kit (Abclonal Cat. # RK04237), Mouse INHB ELISA kit (Abclonal Cat ...
-
bioRxiv - Bioengineering 2021Quote: ... we used MultiF Seamless Assembly Mix kit (ABclonal). DNA fragments for assembly were amplified with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) ...
-
bioRxiv - Cell Biology 2023Quote: ... mitochondrial ELISA kit (SOD2) (ABclonal, Catalog Number-RK04590) as per the manufacturer’s instructions ...
-
bioRxiv - Physiology 2024Quote: ... Quantitative RT-PCR reactions were carried out in a volume of 20 µl of 1X SYBR Green fast qPCR Mix (#RK21200, ABclonal, Woburn, MA), and 100mM primers using CFX96 qPCR machine (Bio-Rad ...
-
NSC-derived exosomes enhance therapeutic effects of NSC transplantation on cerebral ischemia in micebioRxiv - Neuroscience 2022Quote: ... and were detected using the ECL Enhanced Kit (ECL, Abclonal).
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and testosterone levels were detected using an ELISA Kit (ABCLONAL) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2022Quote: ... The Rapid Plus DNA Lib Prep Kit for Illumina (ABclonal, Wuhan, China) was used to prepare the PhoP-bind DNA and INPUT DNA libraries for Illumina sequencing according to the manufacturer ...
-
bioRxiv - Cell Biology 2022Quote: Plasmids were constructed using the 2 × MultiF Seamless Assembly Kit (Abclonal; RK21020). Vector backbones were linearized by DNA restriction endonucleases ...
-
bioRxiv - Neuroscience 2023Quote: ... An enzyme-linked immunosorbent assay (ELISA) kit (#RK03260, Abclonal Technology, Woburn, MA) was used to assess plasma TSH levels ...
-
bioRxiv - Plant Biology 2023Quote: ... Immunodetection was performed using an ECL Enhanced Plus Kit (ABclonal, Wuhan. China) and detected using a ChemiScope 6000 Touch chemiluminescence imaging system (Clinx) ...
-
bioRxiv - Molecular Biology 2024Quote: The ChIP assay was performed using Sonication ChIP Kit (Abclonal, Cat#RK20258). Briefly ...
-
bioRxiv - Cell Biology 2021Quote: ... Granzyme B and perforin were determined by ELISA kits (ABclonal, Biolegend, and Abcam) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... 2 × MultiF Seamless Assembly Kit was used to construct the plasmids (Abclonal; RK21020). Sanger sequencing was performed to confirm sequence accuracy.
-
bioRxiv - Neuroscience 2023Quote: ... qPCR was performed using 2X SYBR Green Fast qPCR Mix kit (ABclonal, cat# RM21203) in a C1000 Touch Thermal Cycler (BioRad ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were generated using mRNA-seq Lib Prep Kit for Illumina (ABclonal, USA, Cat. RK20302) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
-
bioRxiv - Immunology 2023Quote: ... TNFα and IFNγ in the culture supernatant were measured using ELISA kits (ABclonal, Cat#RK00027, Cat#RK00019). Samples were plated in duplicate.
-
bioRxiv - Immunology 2023Quote: ... Supernatants were collected for measurement of TNFα and IFNγ secretion using ELISA kits (ABclonal, Cat#RK00030, Cat#RK00015).
-
bioRxiv - Microbiology 2022Quote: ... The expression of nucleocapsid protein was confirmed by the SARS-CoV-2 Nucleocapsid Protein ELISA Kit (ABclonal, Woburn, MA) and the cell clone supporting the most efficient virus rescue was selected (VeroE6-N) ...
-
bioRxiv - Plant Biology 2023Quote: ... The protein quantification was performed via the Detergent Compatible Bradford Protein Assay Kit (P0006C, Beyotime, China) and Western blotting was performed according to the manufacturer’s protocol from ABclonal (https://abclonal.com/Uploads/protocol/WB_protocol.pdf) ...