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Citations for Abclonal Science :
451 - 500 of 501 citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The dilutions and sources of antibodies used in WB are as following: Cbln1 (1:100, Abclonal), FLAG (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... and anti-SCRN3 against His315-Ser814 (1:10,000, NP_083298.1; custom-made rabbit polyclonal antibody from Abclonal, which we verified for specificity in a SCRN3 HEK-293T overexpression system (Matthews ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies used include phosphorylated nuclear factor kappa B (p-NF-κB, 1:1000 dilution, ABclonal); nuclear factor kappa B (NF-κB ...
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bioRxiv - Biochemistry 2024Quote: ... Alexa Fluor 647-conjugated anti-mouse and Alexa Fluor 488-conjugated anti-rabbit (Abclonal; 1:500) were applied simultaneously in the 3% BSA solution for 1 hours at RT ...
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bioRxiv - Genomics 2023Quote: ... and HHIP was detected using rabbit anti-HHIP polyclonal antibody diluted 1:1500 (ABClonal Science, #A5872). Slides were incubated with primary antibody at 4°C for 16 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were then incubated with a horseradish peroxidase-conjugated secondary antibody (1:5000; ABclonal, China) for two hours at room temperature after being washed three times with TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... these were incubated at 4°C O/N with rabbit anti-MBP (A1664, 1:500, ABclonal), rabbit anti-MOBP (bs-11184R ...
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bioRxiv - Biophysics 2024Quote: ... the cells were incubated with a 1:500 dilution of TNFR1/TNFRSF1A rabbit pAb (ABclonal, #A1540) in antibody dilution buffer (3% BSA in 1x PBS ...
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bioRxiv - Microbiology 2021Quote: Recombinant SARS-CoV-2 Spike RBD (derived from strain Wuhan-Hu-1) protein was purchased from ABclonal Technology and labeled with Alexa Fluor 674 reactive fluorescence dye according to manufacturer’s protocols (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were also incubated with rabbit anti-actin antibody (1:1000, Cat. No. AC026, Abclonal Technology, USA) as a loading control for normalization ...
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bioRxiv - Neuroscience 2023Quote: ... and HRP conjugated goat-derived anti-rabbit or anti-mouse were the antibodies used (1:5000, Abclonal).
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bioRxiv - Developmental Biology 2023Quote: ... The secondary antibodies were HRP-conjugated goat anti-mouse IgG heavy chain (1:10000, ABclonal, Cat #AS064) or HRP-conjugated goat anti-mouse IgG light chain (1:10000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were incubated with a 1:1,000 dilution of HRP Goat Anti-Rabbit IgG (H+L) (ABclonal, AS014) for 2 h and visualized using Tanon imager (Supplementary Fig ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-RMDN3 (also known as PTPIP51; cat. A5820, RRID: AB_2766572; 1:1,000 for IB) antibody was purchased from Abclonal. The horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (cat ...
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bioRxiv - Systems Biology 2024Quote: ... followed by overnight incubation at 4°C in primary antibodies for ITGAV (1:1,000, A2091, Abclonal, Woburn, MA), FBLN5 (1:1,000 ...
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bioRxiv - Physiology 2023Quote: ... Primary antibodies (all diluted 1:1000 for O/N incubation at +4°C): rabbit anti-PGC1alpha (ABClonal #A12348), rabbit anti-GR (ABClonal #A2164) ...
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bioRxiv - Biochemistry 2022Quote: ... and 100uL of primary antibody solution was added and incubated for 1h at room temperature (1:10000 Abclonal A19841 including 0.5ug/mL competing ime4Δ yeast total RNA) ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated with the primary antibody overnight at 4[: anti-β-actin (1:50000, ABclonal, Cat#: AC026), anti-Claudin-1 (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were then incubated overnight at 4 °C with primary antibodies against AKR1B1 (1:2000, A22132, ABclonal), and β-actin (1:5000 ...
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bioRxiv - Plant Biology 2024Quote: ... then incubated with a goat anti-mouse IgG HRP-linked secondary antibody (AS003, ABclonal, China, 1:5,000 dilution) in TBST containing 5% skim milk for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... and then with the secondary antibody HRP labeled goat anti-rabbit IgG (with the dilution of 1:5000, ABclonal) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were exposed to HRP-conjugated goat anti-rabbit secondary antibody (1:6000, Cat. No. AS014, Abclonal Technology, USA) for one hour with signal visualized on a GE Amersham Imager 600 (GE Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... Na+/K+-ATPase was used as loading control for normalization and was detected with rabbit anti-Na+/K+-ATPase monoclonal antibody (1:10,000 dilution) (ABclonal) combined with secondary HRP goat anti-rabbit monoclonal antibody (1:10,000 dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... blots were incubated for 1 hour at room temperature with HRP-conjugated anti-rabbit– or anti-mouse-IgG (Abclonal), diluted at 1:5000 in TBST ...
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bioRxiv - Biochemistry 2024Quote: ... The secondary antibody (Alexa Fluor 647-conjugated anti-mouse and Alexa Fluor 488-conjugated anti-rabbit, Abclonal; 1:500) were used to incubate cells at RT in darkness for 1 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Two MS-identified upregulated proteins in HeLa knockout cells’ nucleoli were detected using IFI16 (ABclonal #A2007, 1:400 dilution) and P14arf (Abcam #ab216602 ...
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bioRxiv - Microbiology 2024Quote: ... A second 1 h incubation at room temperature was performed with secondary anti-Histidine-Tag antibody (ABclonal, MA, USA) and/or anti-Dog IgG antibody (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... one section on each slide was incubated with Flt3 primary antibody at 4°C overnight (Abclonal, A12462, 1:200) and the other section on the same slide was used as the staining control which used the same amount of rabbit IgG to replace the Flt3 rabbit antibody ...
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bioRxiv - Neuroscience 2020Quote: ... blots were exposed to HRP conjugated goat anti-rabbit secondary antibody (1:6000, Cat. No. AS014, Abclonal Technology, United States) for one hour ...
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bioRxiv - Genetics 2022Quote: Two CPC-1 peptides (SELDLLDFATFDGG and RDKPLPPIIVEDPS) were synthesized and used as the antigens to generate rabbit polyclonal antisera (ABclonal) as described previously (Cui et al. ...
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bioRxiv - Immunology 2023Quote: ... Expression of all the target proteins were normalized to that of the internal control rabbit/mouse antibodies against β-actin or GADPH (1:50,000, ABclonal). Relevant DyLight™ 800-labeled goat anti-rabbit/-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μg of RNA was transcribed into complementary DNA (cDNA) using the ABScript III RT-PCR kit (ABclonal Technology, RK20429). The qPCR was performed using an SYBR Green reaction mix (ABclonal Technology ...
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bioRxiv - Biochemistry 2022Quote: ... overnight followed by incubation with a secondary goat HRP-conjugated anti-mouse at 1:10000 dilution in milk (ABclonal, #AS003) and treated with Clarity™ ECL (Bio-Rad) ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked in 5% milk in TBST for 1 hour at room temperature and then incubated overnight at 4°C with primary antibodies: PCSK9 (Cat #A7860, 1:1000, ABclonal), BHLHE40 (Cat #A6534 ...
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bioRxiv - Molecular Biology 2024Quote: ... which was then exposed to 5% dried skim milk for 1 h at room temperature before incubation with primary antibodies including those to PGC-1α (A12348, Abclonal), to UCP1 (sc-293418 ...
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bioRxiv - Pathology 2024Quote: The protein concentration of DNM1 and Flotillin-1 were quantified using commercially available enzyme-linked immunosorbent assay (ELISA) kits (ABclonal RK01275 and FineTest EH1351 respectively ...
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bioRxiv - Bioengineering 2021Quote: ... The membranes were blocked with 5% non-fat milk for 1h and were then incubated overnight at 4°C with primary antibodies: anti-CANX (1:500, rabbit polyclonal anti-human ABclonal, A15631); anti-CD63 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were subsequently incubated ON at 4°C with primary antibodies: rabbit anti-GFP at 1/500 to detect PIEZO2 (Abclonal; AE011), rabbit anti-HA to detect PIEZO1 at 1/1000 (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% nonfat dry milk in PBST for 1 h and then incubated overnight with primary antibodies against FANCI (ABclonal, A3447), DDB2 (ABclonal ...
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bioRxiv - Molecular Biology 2024Quote: ... the tissue slides were incubated at 4 °C condition overnight with the primary antibody of anti-LC3B (1:200, ABclonal Technology) or anti-UCP2 (1:50 ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked with 5 % milk in TBS with 0.1 % Tween20 and incubated with primary antibodies against SETD2 (1:1000, A3194, ABclonal, LOT 0071240201), H3K36me3 (1:5000 ...
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bioRxiv - Physiology 2024Quote: ... The membrane was blocked with 3% BSA in PBS for 30 min at room temperature and then incubated with rabbit anti-Akh antibody (1:1000, Abclonal, A22867) in 3% BSA in PBS at 4 °C for overnight followed by incubation with HRP-conjugated secondary antibodies in 3% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Western blotting was carried out with gradient gel (BCM Biotech, cat. no. P2012) with the following antibodies: Zfp281 (1:3,000, ABclonal, cat. no. A12650), GAPDH (1:20,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were all used at 1:1,000 in blocking buffer (SM2, Abclonal #A4064; SMA Abclonal #A17910; EGFP, Santa Cruz #SC-9996)
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bioRxiv - Developmental Biology 2024Quote: ... or 1 μg (wings and halteres) of RNA using ABScript III RT Master Mix for qPCR with gDNA remover (ABclonal, cat# RK20429). qRT-PCR was performed in a 10 μl reaction system with iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with phosphate-buffered saline containing 5% non-fat dry milk and probed with rabbit polyclonal SARS-CoV-2 S1 antibody (A20136, ABclonal, 1:1000 dilution), guinea pig polyclonal NSP3 (NIH Lot 55068 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the membrane was blocked using 5% skimmed milk and incubated with the following anti-mouse primary antibodies: HIF1α (1:1000, Abclonal, Woburn, MA, USA), ICAM1 (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... The isolated precipitate was boiled with loading buffer at 100°C for 6 min and detected by western blot using anti-His (Abclonal, AE003, China, 1:10000) or anti-GST (Abclonal ...
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bioRxiv - Developmental Biology 2022Quote: ... For western blot analyses the following secondary antibodies conjugated to Horse Radish Peroxidase were used: anti-rabbit IgG HRP-linked (ABclonal, AS014, WB: 1:5000), anti-mouse IgG HRP-linked (ABclonal ...
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bioRxiv - Developmental Biology 2023Quote: ... A rabbit anti-GFP primary antibody was used to detect HA::GFP::RAB-7 and a rabbit anti-CD63/TSP-7 antibody (Abclonal, A5271, 1:500, RRID: AB_2766092) was used to detect CD63/TSP-7.