Labshake search
Citations for New England Biolabs :
351 - 400 of 10000+ citations for ssc mir 17 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2022Quote: ... using the Phusion High-Fidelity PCR Kit (NEB). Resulting products were cloned into the pGL4.23 luciferase reporter vector (Promega ...
-
bioRxiv - Genomics 2023Quote: ... using the Phusion High Fidelity PCR kit (NEB) using primers designed to span the ATAC-seq signals (Table S7 ...
-
bioRxiv - Microbiology 2023Quote: ... The Monarch PCR and DNA Cleanup Kit (NEB) and Monarch DNA Gel Extraction Kit (NEB ...
-
bioRxiv - Bioengineering 2024Quote: ... and a Monarch PCR & DNA Cleanup kit (NEB) with the following changes ...
-
bioRxiv - Biochemistry 2023Quote: ... RL-Con and RL-3X bulge-miR-122 were digested with DraI (New England Biolabs) prior to following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... and 5 μM dNTP in 17 μL 1x rCutSmart buffer (NEB). The RNA and primers were then annealed by incubating as follows ...
-
bioRxiv - Molecular Biology 2022Quote: ... The eluted DNA fragments were then amplified by PCR with Nextera compatible indexed sequencing i5 and i7 adapters using NEBNext 2x PCR Master Mix PCR kit (M0541, NEB). The amplified DNA library was fragment size selected from 200bp to 800bp using Ampure XP beads (A63880 ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was PCR amplified (primers hFUR Exon 1-3 PCR For and hFUR Exon 1-3 PCR Rev) and purified (Monarch PCR & DNA cleanup kit, NEB). The resulting PCR product was sequenced using the hFur Exon 1 Seq primer.
-
bioRxiv - Evolutionary Biology 2023Quote: ... The eluted DNA fragments were then amplified by PCR with Nextera compatible indexed sequencing i5 and i7 adapters using NEBNext 2x PCR Master Mix PCR kit (M0541, NEB). The amplified DNA library was fragment size selected from 200bp to 800bp using Ampure XP beads (A63880 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Single step PCR was performed using a One-Taq™ PCR reaction kit (NEB, USA) with 30 amplification cycles and 2 ng of original circular-DNA extract as the DNA template ...
-
bioRxiv - Synthetic Biology 2019Quote: ... PCR products and digested plasmids were purified with the Monarch PCR & DNA Cleanup Kit (NEB). Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN).
-
bioRxiv - Genetics 2021Quote: ... PCR amplicons were purified using the Monarch PCR & DNA cleanup kit (New England BioLabs T1030) or the QIAQuick PCR purification kit (Qiagen 28104 ...
-
bioRxiv - Developmental Biology 2022Quote: ... followed by 9 cycles of PCR enrichment using NEB High fidelity PCR kit (NEB, M0541S). The quality of ATAC libraries was assessed with Agilent Bioanalyzer with DNA High Sensitivity kit (Agilent Technologies ...
-
bioRxiv - Bioengineering 2022Quote: ... The PCR product was purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs, Inc.). The samples were run on a 1.5% Agarose (Merck ...
-
bioRxiv - Bioengineering 2022Quote: ... PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and then digested by BspHI and MfeI-HF (New England Biolabs) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The resulting PCR product was purified with the Monarch PCR and DNA Cleanup Kit (NEB) with elution in 20 microliters nuclease-free water ...
-
bioRxiv - Molecular Biology 2019Quote: ... PCR products were purified using the Monarch PCR and DNA cleanup kit (New England Biolabs), and electrophoresed on 1 % agarose gel to verify amplicon size.
-
bioRxiv - Microbiology 2019Quote: ... The remaining PCR mixture was purified using the Monarch® PCR & DNA Cleanup Kit (NEB) and the DNA eluted with nuclease-free water ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The PCR products were purified with Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and subcloned using StrataClone PCR cloning Kit (Agilent Technologies ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 6 were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of an oligo that includes a T7 promoter ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified using a Monarch PCR & DNA Cleanup Kit (5 μg) from NEB and then visualized on an 0.8 to 1.2 % percent agarose gel resolved in Tris-acetate-EDTA electrophoresis buffer ...
-
bioRxiv - Bioengineering 2022Quote: ... PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and submitted to the Massachusetts General Hospital DNA Core ...
-
bioRxiv - Genomics 2022Quote: ... The PCRs were then purified with Monarch PCR & DNA Cleanup Kit (New England Biolabs, T1030) and Illumina adapters were added in another 2 rounds of PCR ...
-
bioRxiv - Biochemistry 2023Quote: ... PCR purifications with the Monarch® PCR & DNA cleanup kit (New England Biolabs, Ipswich, USA), extraction of genomic DNA with the Monarch® Genomic DNA Purification Kit (New England Biolabs ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplicons were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs).
-
bioRxiv - Immunology 2024Quote: ... Purified round 2 PCR products were cloned using the NEB PCR Cloning Kit (NEB, #E1202) and sequenced with Sanger sequencing.
-
bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μl of the cDNA was used as template for the RT-PCR reaction with OneTaq DNA Polymerase (New England Biolabs). PCRs were run at the following conditions ...
-
bioRxiv - Plant Biology 2019Quote: ... RT-PCR reaction volumes were set up to 12.5 μL containing Luna Universal qPCR Master Mix (New England Biolabs Inc.), 2 μl of a 1:10 dilution of cDNA reaction ...
-
bioRxiv - Molecular Biology 2022Quote: ... aeruginosa AZPAE12409 was inserted into pBL1MBPG2L4 RT-8XHis by PCR amplifying a 658-bp region of genomic DNA containing the Direct Repeats with Gibson forward and reverse primers that append flanking KpnI sites and inserting the KpnI-digested PCR product into the KpnI site of pBL1-MBPG2L4 RT-8XHis by using NEBuilder HiFi DNA Assembly (New England Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... and rps10 (ENSDARG00000034897) were amplified by RT- PCR and cloned into pCS2+HA using NEBuilder HiFi DNA Assembly (New England BioLabs). The pCS2+HA was generated by inserting synthetic DNA fragments with HA- tag sequence into pCS2+ via EcoRI/XbaI restriction sites ...
-
bioRxiv - Genetics 2023Quote: ... The presence of infectious rIBV in the allantoic fluid was confirmed by a two-step reverse transcription polymerase chain reaction (RT-PCR) protocol using Protoscript II reverse transcriptase (NEB) and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen ...
-
Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... Dual-indexing was carried out with the TruSeq panel of indexing primers in a short-cycle PCR using Luna Universal Probe One-Step RT-qPCR reagents (NEB). The pooled adapter libraries were purified and size-selected using AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Developmental Biology 2020Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
-
Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs, #E3005L or #E3006E) according to manufacturer’s instructions and then analyzed with QuantStudio Design & Analysis Software v1.5.1 (Applied Biosystems ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad).
-
bioRxiv - Neuroscience 2020Quote: RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). To confirm that striatal ChIs or midbrain dopaminergic neurons were collected via LCM ...
-
bioRxiv - Biophysics 2021Quote: ... We used the Luna Universal One-step RT-qPCR kit (E3005S, New England Biolabs, MA) to determine the relative abundance of target mRNA between two samples ...
-
bioRxiv - Molecular Biology 2020Quote: ... according to the manufacturer’s instructions and reverse transcribed with the LunaScript RT SuperMix kit (NEB). cDNA was analyzed by quantitative PCR using the CFX96 Touch Real-Time PCR Detection System (BIO-RAD) ...
-
bioRxiv - Genetics 2021Quote: ... All RNA samples were reverse transcribed using the LunaScript™ RT supermix kit (#E3010L, NEB), according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... using Luna Universal Probe One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: RNA (500 ng) was reverse transcribed using a LunaScript RT SuperMix kit (New England Biolabs) according to the manufacturer’s instructions in a total 20 µL reaction mix ...
-
bioRxiv - Microbiology 2022Quote: ... and 16s rRNA transcripts by the Luna Universal One-Step RT-qPCR kit (NEB, E3005), according to the manufacturer’s instructions on an Applied Biosystems 7500 Fast Real-Time PCR System ...
-
bioRxiv - Immunology 2023Quote: ... Reverse transcription (RT) was performed using ProtoScript First Strand cDNA Synthesis kit (New England Biolabs) to generate cDNAs ...
-
bioRxiv - Biochemistry 2023Quote: ... For assessment of HAC1spliced mRNA the Luna Universal Probe One-Step RT-qPCR Kit (NEB) was used with 2 µl of a 50 ng/ µl RNA sample ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad) ...
-
bioRxiv - Cell Biology 2023Quote: ... using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, USA) and a BIORAD CFX Opus 384 system ...
-
bioRxiv - Plant Biology 2023Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: cDNA synthesis on all RNA samples was carried out using LunaScript RT SuperMix Kit (NEB) with 16 μl template RNA in 20 μl reactions according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... AMV RT (NEB, M0277L) was used for primer extension at 42 ⁰C ...