Labshake search
Citations for New England Biolabs :
301 - 350 of 10000+ citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Secondary PCRs were purified with the Monarch PCR and DNA Cleanup Kit (NEB), ligated into the pCR2.1-TOPO TA cloning vector (Invitrogen) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR products were purified using a Monarch PCR Cleanup Kit (New England Biolabs) and prepared for nanopore long read sequencing using the Ligation Sequencing Kit (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using the Monarch PCR & DNA Cleanup Kit (NEB) and sent for Sanger sequencing (GENEWIZ).
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bioRxiv - Microbiology 2024Quote: ... PCR products were cleaned using the Monarch PCR & DNA cleanup kit (NEB, T1030L). VSG sequences were determined by amplicon nanopore sequencing performed by Plasmidsaurus using Oxford Nanopore Technology with custom analysis and annotation or Sanger sequencing with the sequencing primer (5’-AGAGAATACTAAGCTAGTTGGC-3’ ...
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bioRxiv - Cell Biology 2023Quote: The novel entry modules created for this study were cloned by insertion of PCR-amplified sequences in pJet1.3 or pMiniT 2.0 following manufacturer’s instructions (CloneJET PCR Cloning Kit, Thermo Scientific; NEB® PCR Cloning Kit, NEB). BsaI recognition sites followed by module-specific overhangs were added to each primer used to amplify entry sequences ...
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bioRxiv - Microbiology 2023Quote: ... Quantification is based on real-time SYBR green amplification molecules with specific target primers using Luna® Universal qPCR Master Mix (Biolabs). Relative mRNA expression is calculated using the CFX Manager software (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: The myo-3p::EFF-1 plasmid was constructed by cloning the myo-3 promoter region from myo-3p::mCherry plasmid with Sal I (New England BioLabs Cat#R3138) and Nhe I (ThermoFisher Cat# FD0974 ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcriptase and RT-qPCR reactions were conducted using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, United States). The reaction was performed with 50 ng of total RNA in a total volume of 20 µL ...
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bioRxiv - Cancer Biology 2022Quote: ... First-strand cDNA synthesis was performed using LunaScript RT SuperMix Kit (NEB, cat. E3010S). qPCR was performed on a QuantStudio 3 or StepOnePlus real-time PCR machine using TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was reverse transcribed into cDNA using a LunaScript RT SuperMix Kit (NEB, E3010L) by following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg RNA was reverse transcribed to cDNA using LunaScript RT SuperMix Kit (NEB). qPCR was performed in 20 μl reactions containing diluted cDNA ...
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bioRxiv - Microbiology 2023Quote: ... RNA immunoprecipitations and inputs were converted to cDNA using LunaScript RT SuperMix Kit (NEB). m6A-immunoprecipitated samples were normalised to their respective input samples and m6A content at a particular region calculated relative to an unmodified control region within the same transcript.
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bioRxiv - Genetics 2023Quote: ... Reactions were performed using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005L) and a CFX Opus 384 Real-Time PCR System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... The Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Cat # E3005X) and reaction protocol (cycle variations ...
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... cDNA was generated for all samples using LunaScript RT SuperMix Kit (New England BioLabs). Sufficient volume was prepared to perform serial dilutions (10−2 to 10 -7 ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was converted to cDNA using LunaScript RT SuperMix Kit (NEB, cat. no. E3010) according to manufacturer specifications ...
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bioRxiv - Genetics 2023Quote: ... We performed cDNA synthesis from RNA using LunaScript RT Supermix Kit (New England Biolabs) beginning with 500ng of RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA (>300 ng) was reverse transcribed using the Lunascript RT Supermix Kit (NEB, E3010).
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bioRxiv - Developmental Biology 2019Quote: ... Genotyping was performed by agarose gel electrophoresis-mediated detection following PCR reaction by Taq polymerase (M0273, New England Biolabs). qPCR was performed using GoTaq® qPCR Master Mix (A6001 ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were column-purified (Monarch PCR & DNA Clean-up Kit, New England BioLabs) and submitted for Sanger sequencing (Genewiz ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified (Monarch® PCR & DNA Cleanup Kit 5µg; New England BioLabs) and sequenced from both sides with Eurofins Genomics ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR was performed on plasmid DNA with Phusion High Fidelity PCR Kit (NEB). PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product was purified using the Monarch PCR & DNA clean-up kit (NEB). All library preparation and sequencing was carried out at the Vanderbilt Technologies for Advanced Genomics (VANTAGE ...
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bioRxiv - Bioengineering 2022Quote: ... PCR products were column-purified (Monarch PCR & DNA Clean-up Kit, New England BioLabs) and submitted for Sanger sequencing (Genewiz ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were column-purified (Monarch PCR & DNA Clean-up Kit, New England BioLabs) and submitted for Sanger sequencing (Genewiz ...
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bioRxiv - Immunology 2023Quote: ... The VHH specific sequence was amplified using PCR (Phusion High Fidelity PCR kit, NEB) and correct overhangs were incorporated ...
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bioRxiv - Systems Biology 2024Quote: ... PCR products were spin-purified using the Monarch PCR & DNA Cleanup Kit (NEB T1030) or the DNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Genomics 2023Quote: ... PCR was performed with the Phusion High-Fidelity PCR kit (E0553L, New England BioLabs) with the following cycling conditions ...
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bioRxiv - Microbiology 2023Quote: ... the PCR reaction was purified using the Monarch PCR Cleanup Kit (New England Biolabs). DNA was sent for Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: PCR products were routinely purified using Monarch® PCR & DNA clean-up kit (NEB), or E.Z.N.A.® Cycle Pure Kit (Omega Bio-Tek ...
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bioRxiv - Biophysics 2019Quote: ... or pMiniT (NEB PCR cloning kit, Cat. # E1202S) plasmids and sequenced ...
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bioRxiv - Microbiology 2019Quote: ... PCRs were performed using Q5 polymerase kit (NEB) in a Perkin- MJ Research PTC-200 Peltier Thermal Cycler or C1000 Touch™ Thermal Cycler (BioRad) ...
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bioRxiv - Genetics 2020Quote: ... and purified using a PCR purification kit (NEB).
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bioRxiv - Biochemistry 2021Quote: ... and a Monarch PCR & DNA Cleanup kit (NEB) with the following changes ...
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bioRxiv - Genomics 2022Quote: ... using the Phusion High-Fidelity PCR Kit (NEB). Resulting products were cloned into the pGL4.23 luciferase reporter vector (Promega ...
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bioRxiv - Genomics 2023Quote: ... using the Phusion High Fidelity PCR kit (NEB) using primers designed to span the ATAC-seq signals (Table S7 ...
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bioRxiv - Microbiology 2023Quote: ... The Monarch PCR and DNA Cleanup Kit (NEB) and Monarch DNA Gel Extraction Kit (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... and a Monarch PCR & DNA Cleanup kit (NEB) with the following changes ...
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bioRxiv - Biochemistry 2023Quote: ... RL-Con and RL-3X bulge-miR-122 were digested with DraI (New England Biolabs) prior to following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted DNA fragments were then amplified by PCR with Nextera compatible indexed sequencing i5 and i7 adapters using NEBNext 2x PCR Master Mix PCR kit (M0541, NEB). The amplified DNA library was fragment size selected from 200bp to 800bp using Ampure XP beads (A63880 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The eluted DNA fragments were then amplified by PCR with Nextera compatible indexed sequencing i5 and i7 adapters using NEBNext 2x PCR Master Mix PCR kit (M0541, NEB). The amplified DNA library was fragment size selected from 200bp to 800bp using Ampure XP beads (A63880 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was PCR amplified (primers hFUR Exon 1-3 PCR For and hFUR Exon 1-3 PCR Rev) and purified (Monarch PCR & DNA cleanup kit, NEB). The resulting PCR product was sequenced using the hFur Exon 1 Seq primer.
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bioRxiv - Molecular Biology 2021Quote: ... Single step PCR was performed using a One-Taq™ PCR reaction kit (NEB, USA) with 30 amplification cycles and 2 ng of original circular-DNA extract as the DNA template ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products and digested plasmids were purified with the Monarch PCR & DNA Cleanup Kit (NEB). Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN).
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bioRxiv - Genetics 2021Quote: ... PCR amplicons were purified using the Monarch PCR & DNA cleanup kit (New England BioLabs T1030) or the QIAQuick PCR purification kit (Qiagen 28104 ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by 9 cycles of PCR enrichment using NEB High fidelity PCR kit (NEB, M0541S). The quality of ATAC libraries was assessed with Agilent Bioanalyzer with DNA High Sensitivity kit (Agilent Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR product was purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs, Inc.). The samples were run on a 1.5% Agarose (Merck ...
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bioRxiv - Bioengineering 2022Quote: ... PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and then digested by BspHI and MfeI-HF (New England Biolabs) ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting PCR product was purified with the Monarch PCR and DNA Cleanup Kit (NEB) with elution in 20 microliters nuclease-free water ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were purified using the Monarch PCR and DNA cleanup kit (New England Biolabs), and electrophoresed on 1 % agarose gel to verify amplicon size.