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Citations for New England Biolabs :
351 - 400 of 10000+ citations for rno mir 137 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were purified using the Monarch PCR and DNA cleanup kit (New England Biolabs), and electrophoresed on 1 % agarose gel to verify amplicon size.
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bioRxiv - Microbiology 2019Quote: ... The remaining PCR mixture was purified using the Monarch® PCR & DNA Cleanup Kit (NEB) and the DNA eluted with nuclease-free water ...
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bioRxiv - Synthetic Biology 2020Quote: ... The PCR products were purified with Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and subcloned using StrataClone PCR cloning Kit (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... 6 were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of an oligo that includes a T7 promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified using a Monarch PCR & DNA Cleanup Kit (5 μg) from NEB and then visualized on an 0.8 to 1.2 % percent agarose gel resolved in Tris-acetate-EDTA electrophoresis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and submitted to the Massachusetts General Hospital DNA Core ...
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bioRxiv - Genomics 2022Quote: ... The PCRs were then purified with Monarch PCR & DNA Cleanup Kit (New England Biolabs, T1030) and Illumina adapters were added in another 2 rounds of PCR ...
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bioRxiv - Biochemistry 2023Quote: ... PCR purifications with the Monarch® PCR & DNA cleanup kit (New England Biolabs, Ipswich, USA), extraction of genomic DNA with the Monarch® Genomic DNA Purification Kit (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplicons were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs).
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bioRxiv - Immunology 2024Quote: ... Purified round 2 PCR products were cloned using the NEB PCR Cloning Kit (NEB, #E1202) and sequenced with Sanger sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of the cDNA was used as template for the RT-PCR reaction with OneTaq DNA Polymerase (New England Biolabs). PCRs were run at the following conditions ...
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bioRxiv - Plant Biology 2019Quote: ... RT-PCR reaction volumes were set up to 12.5 μL containing Luna Universal qPCR Master Mix (New England Biolabs Inc.), 2 μl of a 1:10 dilution of cDNA reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... aeruginosa AZPAE12409 was inserted into pBL1MBPG2L4 RT-8XHis by PCR amplifying a 658-bp region of genomic DNA containing the Direct Repeats with Gibson forward and reverse primers that append flanking KpnI sites and inserting the KpnI-digested PCR product into the KpnI site of pBL1-MBPG2L4 RT-8XHis by using NEBuilder HiFi DNA Assembly (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and rps10 (ENSDARG00000034897) were amplified by RT- PCR and cloned into pCS2+HA using NEBuilder HiFi DNA Assembly (New England BioLabs). The pCS2+HA was generated by inserting synthetic DNA fragments with HA- tag sequence into pCS2+ via EcoRI/XbaI restriction sites ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... Dual-indexing was carried out with the TruSeq panel of indexing primers in a short-cycle PCR using Luna Universal Probe One-Step RT-qPCR reagents (NEB). The pooled adapter libraries were purified and size-selected using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... The presence of infectious rIBV in the allantoic fluid was confirmed by a two-step reverse transcription polymerase chain reaction (RT-PCR) protocol using Protoscript II reverse transcriptase (NEB) and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs, #E3005L or #E3006E) according to manufacturer’s instructions and then analyzed with QuantStudio Design & Analysis Software v1.5.1 (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). To confirm that striatal ChIs or midbrain dopaminergic neurons were collected via LCM ...
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bioRxiv - Biophysics 2021Quote: ... We used the Luna Universal One-step RT-qPCR kit (E3005S, New England Biolabs, MA) to determine the relative abundance of target mRNA between two samples ...
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bioRxiv - Molecular Biology 2020Quote: ... according to the manufacturer’s instructions and reverse transcribed with the LunaScript RT SuperMix kit (NEB). cDNA was analyzed by quantitative PCR using the CFX96 Touch Real-Time PCR Detection System (BIO-RAD) ...
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bioRxiv - Genetics 2021Quote: ... All RNA samples were reverse transcribed using the LunaScript™ RT supermix kit (#E3010L, NEB), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... using Luna Universal Probe One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: RNA (500 ng) was reverse transcribed using a LunaScript RT SuperMix kit (New England Biolabs) according to the manufacturer’s instructions in a total 20 µL reaction mix ...
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bioRxiv - Microbiology 2022Quote: ... and 16s rRNA transcripts by the Luna Universal One-Step RT-qPCR kit (NEB, E3005), according to the manufacturer’s instructions on an Applied Biosystems 7500 Fast Real-Time PCR System ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription (RT) was performed using ProtoScript First Strand cDNA Synthesis kit (New England Biolabs) to generate cDNAs ...
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bioRxiv - Biochemistry 2023Quote: ... For assessment of HAC1spliced mRNA the Luna Universal Probe One-Step RT-qPCR Kit (NEB) was used with 2 µl of a 50 ng/ µl RNA sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, USA) and a BIORAD CFX Opus 384 system ...
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bioRxiv - Microbiology 2023Quote: cDNA synthesis on all RNA samples was carried out using LunaScript RT SuperMix Kit (NEB) with 16 μl template RNA in 20 μl reactions according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... AMV RT (NEB, M0277L) was used for primer extension at 42 ⁰C ...
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bioRxiv - Genetics 2024Quote: ... 10x RT buffer (NEB) and 10mM dNTP mix (NEB) ...
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bioRxiv - Genomics 2021Quote: ... PCR reaction according to the manufacturer’s protocol (annealing temperature: 60°C; elongation time: 15sec, 19 cycles) (NEB). Of this reaction ...
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bioRxiv - Developmental Biology 2019Quote: ... and the resulting fragment was restriction digested and cloned into pME-miR using T4 ligation (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the piggy-bac vector pPBhCMV1-miR(BsgI)-pA-3 was digested with BsgI (#R05559S, NEB) and the digested vector excised from a DNA agarose gel and the DNA purified ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg/ml glycogen) and DNA purification was carried out with the PCR cleanup kit (NEB, Monarch PCR & DNA Cleanup Kit, T1030). About 10ng of the purified CUT&RUN DNA was used for preparation of multiplexed libraries with the NEB Ultra II DNA Library Prep Kit per manufacturer’s instruction (NEB #E7103) ...
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bioRxiv - Plant Biology 2020Quote: ... respectively by Phusion® High-Fidelity PCR Kit (NEB) (Harashima & Schnittger ...
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bioRxiv - Microbiology 2019Quote: PCR fragments were assembled using Gibson assembly kit (NEB) and verified by Sanger sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... purified with the Monarch PCR & DNA Cleanup Kit (NEB) and ligated with T4 DNA ligase (NEB) ...
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bioRxiv - Cancer Biology 2021Quote: ... a PCR-based Q5 site-directed mutagenesis kit (NEB) was used according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... and then purified with the Monarch PCR kit (NEB). PCR amplicons were digested using MfeI-HF and SphI-HF (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... coli using the NEB® PCR Cloning Kit (NEB).
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bioRxiv - Microbiology 2022Quote: ... and purified using Monarch PCR clean-up kit (NEB). Blunted DNA was A-tailed using NEBNext dA-tailing kit (NEB ...
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bioRxiv - Biophysics 2022Quote: ... and Q5 High-Fidelity PCR Kit (New England BioLabs). Plasmids were transformed in E ...
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bioRxiv - Bioengineering 2020Quote: ... Q5 High-Fidelity PCR Kit was purchased from NEB.
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bioRxiv - Molecular Biology 2021Quote: ... purified with the Monarch PCR & DNA Cleanup Kit (NEB) and ligated with T4 DNA ligase (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... PCRs were performed using Q5 DNA polymerase kit (NEB) with the appropriate primer pair and 25 ng of plasmid as template (segment 1 of PR8 ...