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Citations for New England Biolabs :
451 - 500 of 10000+ citations for rno mir 101a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... After PCR amplification or plasmid digestion product was purified with Monarch PCR clean-up kit (NEB, #T1030s) and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR was performed with 2 μl of the reverse transcription positive reactions and Phusion High Fidelity DNA polymerase (NEB, Ipswich, MA) following manufacturer’s protocols on an Applied Biosystems 2720 Thermal Cycler ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... purified with the Monarch PCR and DNA Cleanup kit (NEB), and then dialyzed with 0.5X TE buffer using a 0.025 μm VSWP membrane (Millipore ...
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bioRxiv - Genomics 2021Quote: ... After purification with Monarch PCR & DNA Cleanup kit (NEB, #T1030S), libraries were indexed using NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was purified using a Monarch PCR purification kit (NEB). qPCR for three independent replicates was performed using iQ SYBR Green Supermix (Biorad ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB). Collected DNA was quantified with the Qubit dsDNA High Sensitivity Assay kit (ThermoFisher) ...
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bioRxiv - Synthetic Biology 2019Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB). Note that a complete digestion of receiver vectors in both step 1 and 2 is key to avoid the recovery of empty backbones ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA was purified with Monarch PCR & DNA Cleanup Kit (NEB) and eluted in 20 µL of nuclease-free water ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and cleaned with the Monarch PCR & DNA cleanup kit (NEB). Pooled libraries were sent to the Beijing Genomics Institute (BGI ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified with the Monarch PCR & DNA Cleanup Kit (NEB). The pLentiGuide-Blast plasmid was digested with BsmBI-V2 (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... They were introduced by mutagenic PCR (Q5 mutagenesis kit, NEB) of the BtubAB expressing plasmid (Schlieper et al ...
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bioRxiv - Synthetic Biology 2022Quote: ... After purification using Monarch® PCR & DNA Cleanup kit (NEB), the templates were transcribed ...
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bioRxiv - Plant Biology 2023Quote: ... and purified using Monarch PCR & DNA Cleanup kit (NEB #T1030). The proUBE2:mTurquoise-N7 ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products were purified with a DNA purification kit (NEB) and were then phosphorylated using T4 polynucleotide kinase (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB). The gRNAs were synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... or Monarch PCR and DNA Cleanup Kit (New England Biolabs).
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bioRxiv - Evolutionary Biology 2023Quote: ... We used the Monarch PCR and DNA Cleanup Kit (NEB) to purify PCR products ...
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bioRxiv - Developmental Biology 2024Quote: The PCR-based Q5 Site-Directed Mutagenesis Kit (NEB, #E0554S) was used to perform truncation (constructs L-P ...
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bioRxiv - Genomics 2021Quote: ... 1μl RT Adapter (110nM; ONT Kit) and 1.5μl T4 DNA Ligase (2M U/ml New England BioLabs). The ligation reaction was carried out for 10min at RT ...
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bioRxiv - Plant Biology 2019Quote: ... ZymoResearch) and reverse-transcribed in the presence of random hexamers (LunaScript®RT SuperMix Kit, NEB Inc.) in 20 μl of total reaction volume containing 1 μg of total RNA and incubated at 55°C for 15 minutes following manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... RNA was then reverse transcribed to cDNA using LunaScript RT SuperMix kit (Catalog # E3010L, New England BioLabs). w1118 flies might have partial or truncated transcripts of w+ ...
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bioRxiv - Bioengineering 2020Quote: ... We prepared cDNA from 1 μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB, #E3010). No Template and No Reverse Transcriptase controls (NTC and NRT ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was generated for all serially diluted RNA samples using LunaScript RT SuperMix Kit (New England BioLabs). Sufficient volume was prepared to perform duplicates for each method at each dilution ...
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bioRxiv - Microbiology 2021Quote: ... purified RNA (8 μl) was converted into cDNA using a LunaScript RT SuperMix Kit (New England Biolabs) and used as a template in two separate amplification reactions generating odd- and even-numbered tiled amplicons ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and IFIH1 were quantified using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs #E3005L) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The one-step cDNA synthesis was carried out using LunaScript™ RT SuperMix Kit (NEB Biolabs, USA) followed by qRT-PCR master mix preparation using Luna® Universal qPCR Master Mix (NEB Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... The one-step cDNA synthesis was carried out using LunaScript™ RT SuperMix Kit (NEB Biolabs, USA) followed by qRT-PCR master mix preparation using Luna® Universal qPCR Master Mix (NEB Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: Samples with CT <25 were submitted to cDNA synthesis protocol using LunaScript™ RT SuperMix Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Comparable total RNA quantities were used for reverse transcription with LunaScript RT SuperMix Kit (New England Biolabs). cDNA was amplified with Phusion polymerase for 30-36 cycles and resulting PCR products were separated on a standard 1% agarose gel next to a 100 bp ladder (NEB) ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized for qPCR quantification using the LunaScript RT SuperMix Kit (New England Biolabs; Massachusetts, USA). qPCR reactions were composed of 7.5 μL Power SYBR Green PCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNAse treatment was performed as described above and cDNA was synthesized using LunaScript RT Supermix Kit (NEB). RT and non-RT vials were checked by PCR with primers 7,8 for PgV (Supplementary File 2) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA were synthesised from 1 µg RNA by reverse transcription using LunaScript RT SuperMix Kit (NEB; E3010). qRT-PCR was performed using Luna Universal qPCR Master Mix (NEB ...
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bioRxiv - Microbiology 2022Quote: ... the RNA was reverse transcribed using the LunaScript RT Supermix Kit (New England BioLabs, NEB; Ipswich, MA). Tiled 1200 bp amplicons were generated using midnight primers (IDT ...
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bioRxiv - Microbiology 2022Quote: ... the RNA was reverse transcribed using the LunaScript RT Supermix Kit (New England BioLabs, NEB; Ipswich, MA). Tiled 1200 bp amplicons were generated using midnight primers (IDT ...
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bioRxiv - Neuroscience 2023Quote: ... 400ng of total RNA was used for reverse transcription using LunaScript™ RT SuperMix Kit (NEB, USA). qPCR was performed using Forget-Me-Not™ EvaGreen® qPCR Master Mix (Biotium ...
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription for first-strand cDNA synthesis was performed by using the LunaScript RT SuperMix Kit (NEB) containing both oligo(dT ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 1 µg of total RNA using the LunaScript RT SuperMix kit (NEB, UK) in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: We performed RT-qPCR on select genes using the Luna Universal One-Step Kit (New England Biolabs) and QuantStudio 5 (Thermo) ...
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bioRxiv - Microbiology 2023Quote: cDNA was synthesized from extracted viral RNA using the LunaScript® RT SuperMix Kit (NEB, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... First strand cDNA synthesis was performed with LunaScript RT SuperMix Kit (E3010L; New England Biolabs, Ipswich, MA), and 100 ng-1 μg total RNA was used for one 20 μl RT reaction ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was reverse transcribed from 1 µg of total RNA using LunaScript RT Supermix kit (NEB #E3010) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... 2 μg of total RNA was converted into cDNA with LunaScript RT SuperMix kit (New England Biolabs). The cDNAs were used as templates for real-time PCR and ran on StepOnePlus real-time PCR system (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription reactions were performed using a LunaScript RT SuperMix Kit (#E3010L, New England Biolabs, Ipswich, MA) with 500 ng of total RNA ...
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bioRxiv - Neuroscience 2022Quote: ... The RT-qPCR was performed using Luna One Step RT-qPCR mix with dUDG (NEB). Amplification using Luna One Step qPCR mix with UDG (NEB ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were pooled and purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Sequenced PCR products showing overlapping peaks in their chromatograms were cloned using NEB PCR cloning kit (NEB #E1202S) and then sequenced to define the identity of mutations carried by F1 heterozygous animals ...
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bioRxiv - Microbiology 2019Quote: ... the PCR products were purified using Monarch® PCR & DNA Cleanup Kit (5 μg; New England Biolabs GmbH). For the second PCR ...
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bioRxiv - Biophysics 2022Quote: ... PCR cleanup was carried out on each amplicon sample using the Monarch PCR cleanup kit (New England Biolabs). The amplicons were then dual-indexed for paired-end read Illumina sequencing using Nextera primers N701 and N702 for the PEL and SUP of the 5.5 h time point and N705 and N706 for the PEL and SUP of the 9.0 h time point ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription and qRT-PCR analysis was performed by Luna universal one- step qRT-PCR kit (NEB # E3005L) with primers list in Table 9.