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Citations for New England Biolabs :
251 - 300 of 6950 citations for mmu mir 212 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cDNA library was PCR amplified using KAPA HiFi HotStart ReadyMix (FisherScientific, 50-196-5217) and NEBNext® Multiplex Oligos for Illumina® Dual Index Primers Set 1 (NEB, E7600S). The library was purified twice using 1x AMPure XP Bead (BeckmanCoulter ...
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bioRxiv - Evolutionary Biology 2024Quote: We prepared dual indexed libraries according to the instruction manual using the NEBNext Ultra II DNA Library Prep Kit for Illumina and the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1, New England Biolabs, Ipswich, Massachusetts, USA) and following the recommended conditions of bead-based size selection according to distribution of DNA fragments per sample ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative RT-PCR analysis was performed using Luna® Universal qPCR Master Mix (NEB) and Rotor-Gene Q (Qiagen) ...
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bioRxiv - Systems Biology 2022Quote: ... PCRs were performed using the Luna® Universal One Step RT-qPCR Kit (NEB) in a ThermoFisher Quantstudio 3 instrument ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA was amplified by RT-PCR (Phusion High Fidelity Polymerase kit, New England Biolabs) using primers listed in Methods S3.
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bioRxiv - Genomics 2020Quote: ... and RT-PCR was performed with Phusion High Fidelity DNA polymerase master mix (NEB) each with the manufacturer recommended PCR thermal cycles ...
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bioRxiv - Microbiology 2024Quote: ... the NEB OneTaq® One-Step RT-PCR Kit (New England Biolabs, Product # E5315S) was used to target the haemagglutinin gene ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μL RNA was mixed with 2 μL of 100 μM PvG748-SBS12-RT random hexamer primer (IDT) and 1 μL of 10 mM dNTP mix (NEB). The sample was incubated for 3 min at 65 °C and transferred to ice ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was prepared from total RNA using the rflP specific primer (P-LCM621) and template-switching oligonucleotide (P-LCM619) using RT Template Switching Enzyme Mix (New England Biolabs). The 5’-end end of the rflP cDNA was enriched by PCR using the primers P-LCM620 and P-LCM622 ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was mixed with gene-specific primers and subjected to RT-qPCR using Hot Start Taq-based Luna qPCR master mix (NEB). The reaction was run on a Bio-Rad CFX Real Time PCR system ...
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bioRxiv - Microbiology 2022Quote: ... Presence of SARS-CoV-2 RNA was determined by using CDC primers and probes with LunaScript RT Supermix Kit (NEB) run on BioRad (Hercules ...
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bioRxiv - Synthetic Biology 2023Quote: ... we first mixed 10.5 uL of purified RNA with 2 uL of 2 uM RT primer (AGGGACATCGTAGAGAGTCGTACTTANNNNNNNNNNAGATGAACTTCAGGGTCAGC, where Ns comprise the UMI) and 2uL of 10mM dNTP mixture (NEB), incubated the mixture at 65C for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 10 μM SARS-CoV-2 E-gene Forward (5’- ACAGGTACCTTAATAGTTAATAGCGT-3’) and Reverse (5’-ATATTGCAGCAGTACGCACACA-3’) primers were used with Luna Universal One-step RT-qPCR kit (New England Biolabs) and added to 1 μl supernatant (5μl total ...
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bioRxiv - Immunology 2024Quote: Reverse-transcription on total RNA (100 to 500 ng) was performed with random hexamer/oligo-dT-Primer containing LunaScript RT Supermix (New England Biolabs) following the manufactureŕs instructions ...
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bioRxiv - Genomics 2020Quote: ... and pre-amplified by PCR in the presence of adapter primers (NEBNext 2x PCR Master Mix, New England Biolabs Inc. #M0541S). Amplification was monitored by RT-PCR using the PowerUp™ SYBR™ Green Master Mix (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... A 2.3 kb amplicon including the NAC18.1 gene and ∼800 bp of upstream sequence was amplified by PCR using primers NAC18F2 and NAC18R2 (Table S1) and Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB). PCR product size and purity was confirmed by agarose gel electrophoresis ...
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bioRxiv - Biochemistry 2022Quote: ... UPF2L (120-1227) were produced by PCR amplification (primers listed in Supplementary Table S1) using the Phusion High-Fidelity PCR kit (NEB #E0553S) prior to restriction digest and ligation into pPROEX-HTB (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... and amplicons were generated by PCR (primers 341F and 806R) using a Phusion® High-Fidelity PCR Master Mix (New England Biolabs). Amplification product quality was assessed by gel electrophoreses and samples were pooled in equimolar ratios ...
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bioRxiv - Genomics 2019Quote: ... were PCR-amplified (2 PCR reactions, 12 cycles) using Illumina adapter-specific primers and NEBNext® Ultra II Q5 Master Mix (NEB). After library profile analysis by Agilent 2100 Bioanalyser (Agilent Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... 2.5 µl 25 µM Custom Nextera PCR Primer 2 and 25 µl NEB Next High Fidelity 2x PCR Master Mix (NEB, #M0541) with 1 cycle of (72°C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl of DNA isolate was used for PCR (primers are in supplementary table 1.4) and PCR cleanup was performed using Exo-SAP (NEB, M0293, M0371). Sanger Sequencing reactions were prepared using (Thermo Fisher # 4337455) ...
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bioRxiv - Developmental Biology 2023Quote: ... using Custom Nextera PCR primer and the NEBNext High-Fidelity 2x PCR Master Mix (Nextera DNA Library Preparation Kit (New England BioLabs, E7530L) and purified with the Qiagen MinElute Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... ATAC-seq libraries were then prepared using Custom Nextera PCR primers and NEBNext High-Fidelity 2x PCR Master Mix (NEB, #M0541).
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bioRxiv - Immunology 2021Quote: ... Pairs of functional Ig genes were PCR-amplified using specific primers with Q5 Polymerase (NEB). PCR-product and linearized vector containing the constant part of IgG1 heavy or kappa/lambda light chain sequences respectively were assembled using Gibson cloning with HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Bioengineering 2020Quote: ... PCR was performed using synthesized primers (Integrated DNA Technologies) and Q5 DNA polymerase (NEB, M0491). The sequences of the constructed plasmids were verified by Sanger sequencing (Genewiz).
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bioRxiv - Genomics 2021Quote: ... The template was PCR amplified (Taq 2x master mix, NEB M0270; Bridge primers; 15 cycles) and size selected by 2% agar gel.
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bioRxiv - Biophysics 2022Quote: ... These filled-in primers are then amplified by PCR with One-Taq DNA polymerase (NEB) and two 21-base primers (HTOP and HBOT ...
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bioRxiv - Immunology 2020Quote: ... Pairs of functional Ig genes were PCR-amplified using specific primers with Q5 Polymerase (NEB). PCR-product and linearized vector were assembled using Gibson cloning with HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Genomics 2020Quote: ... Using primers 642F and 643R and NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541L), PCR was run for 10 cycles with the following parameters ...
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bioRxiv - Bioengineering 2021Quote: ... PCR primers were designed with 20-25bp of overlap and Gibson assembled (New England Biolabs) and electroporated into E ...
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bioRxiv - Bioengineering 2023Quote: ... PCR was performed using synthesized primers (Integrated DNA Technologies) and Q5 DNA polymerase (NEB, M0491). The sequences of the constructed plasmids were verified by Sanger sequencing (Azenta) ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... PCR amplification was performed with the specific primers and OneTaq master mix (New England Biolabs) under the following condition ...
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bioRxiv - Biochemistry 2024Quote: ... The purified PCR product was digested by KpnI and XhoI (NEB, underlined in primer sequences) and repurified ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were constructed by PCR with the index primers for the EM-seq kit (NEB) with NEBNext Q5U Master Mix (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 6×50 µl PCR reactions were set up using NEBNext Ultra II Q5 master mix (New England Biolabs) with 5 nM oligos as template ...
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bioRxiv - Plant Biology 2020Quote: ... PCR enrichment of adaptor ligated DNA was conducted using NEBNext Multiplex Oligos for Illumina (Set 1, NEB#E7335). The PCR reaction was purified using Agencourt AMPure XP Beads ...
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bioRxiv - Immunology 2022Quote: ... PCR reaction was set up according to NGS protocol of NEBNext UltraTM II Q5 Master Mix (NEB, Cat.M0544S). PCR products were purified by AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2019Quote: Shotgun libraries for Illumina MiSeq sequencing were prepared with the NEBNext® Ultra II DNA Library Prep Kit for Illumina® in combination with the Index Primer Set 1 (NEB, Frankfurt, Germany) according to manufacturer’s instructions and as described in (57) ...
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bioRxiv - Cancer Biology 2022Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB, Set 1 #E7335S, Set 2 #E7500S). ChIP libraries were done following NEB’s guidelines (NEB ...
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bioRxiv - Microbiology 2021Quote: The synthesized oligo pool of CRISPRa library was PCR amplified with primers (table S5) including the BsmBI recognition sites using Phusion®High-Fidelity PCR Kit (NEB, E0553L). After purification with DNA Clean & Concentrator-25 (Zymo Research Corporation ...
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bioRxiv - Bioengineering 2021Quote: ... qRT-PCR was performed with primers specific for target genes (see Supplementary Table 1 for the list of primers) using the Luna universal One-Step RT-qPCR kit (NEB; E3005). Experiment was performed using the QuantStudio3 (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... qRTPCR was performed with primers mentioned in Table S3 with Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs). Log2 normalized RNA abundances were calculated using ValidPrime signal as reference (51).
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bioRxiv - Microbiology 2021Quote: ... Yields of viral RNA were quantified by real-time qPCR by using SARS-CoV-2 specific primers targeting the E gene with the Luna®Universal One-Step RT-qPCR Kit (New England Biolabs) in a LightCycler 480 thermocycler (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of RNA were mixed with 2 μl of 100 μM PvG748-SBS12-RT random hexamer primer (Supplementary Table 3) and 1 μl of 10 mM dNTP mix (NEB, N0447S), incubated for 3 minutes at 65 °C and transferred to ice ...
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bioRxiv - Biochemistry 2023Quote: ... Ligated fragments were separated from free linkers on a 15% polyacrylamide TBE-Urea gel and then pooled and purified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB; M0368S). The ∼105 nt cDNAs were separated from free RT primers on a 15% TBE-Urea gel and circularized using CircLigaseII ssDNA Ligase (Biosearch Technologies ...
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bioRxiv - Systems Biology 2024Quote: ... 3 µl of 100 µM barcode-linked oligo-dT primer was added to each well with 5 µl of 5x ProtoScript RT buffer (New England Biolabs M0368). The plate was incubated at 94°C for 2 min and immediately cooled on ice for at least 5 min ...
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bioRxiv - Bioengineering 2024Quote: cDNA of each library was generated using oAS345 (Supplemtary Note 21) and LunaScript Primer-Free RT Master Mix Kit (NEB E3025S).
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bioRxiv - Neuroscience 2021Quote: ... Genes of interest were amplified with the One Taq One-Step RT-PCR kit (NEB) using 1 μg of RNA ...
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bioRxiv - Plant Biology 2021Quote: ... and reverse-transcribed following manufacturer’s recommendations (The ProtoScript Taq RT-PCR Kit, New England Biolabs). Relative mRNA ...
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bioRxiv - Immunology 2021Quote: ... and 60 ng were used for the One Taq RT PCR Kit (New England Biolabs) according to the instructions provided by the manufacturer for mRNA quantification and reverse transcription (Thermo Fisher Scientific ...