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Citations for New England Biolabs :
101 - 150 of 6947 citations for hsa mir 30b RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... directly into One Step RT-PCR reaction mix (NEB) loaded in MicroAmp Optical 96-well reaction plates (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: PCR with the teltail and P1 PCR primers was performed using Q5 PCR enzyme mix (NEB, #M0494) and the following conditions using 0.1 μl DNA-bound beads ...
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bioRxiv - Biochemistry 2021Quote: ... Extra RT primers were removed by Exonuclease I (100 U; M0293S, NEB, Ipswich, MA) for 2 hr at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... rRNA was reverse transcribed using a linker-specific primer and Protoscript II RT (NEB) to generate the first strand of cDNA ...
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bioRxiv - Microbiology 2023Quote: ... reverse transcription was carried out using 10µM oligo dT primer and MMLV RT (NEB) according to the manufacture protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... Eight separate 50 μL PCR reactions were set up with Q5 (NEB) using 2.5 μL of the diluted midiprep as the template and with the primers Orange_NGS_F and R ...
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bioRxiv - Biophysics 2019Quote: ... PCR reactions were set up using 2x Q5 mastermix (NEB, cat# M0492), 10 ng of plasmid template ...
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bioRxiv - Biophysics 2021Quote: PCR reactions were set up using 2x Q5 mastermix (NEB, cat#M0492), 10 ng of plasmid template ...
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bioRxiv - Cancer Biology 2021Quote: ... NEBNext® Multiplex Oligos for Illumina® Dual Index Primers Set 1 (cat. #E7600, New England Biolabs), and AMPure® XP Beads (cat ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Sequences were indexed using the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1, NEB #E7600). A series of sequencing runs were performed on Illumina and PacBio platforms ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Sequences were indexed using the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1, NEB #E7600). We sequenced paired end libraries at the Institute for Biotechnology at Cornell University ...
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bioRxiv - Cancer Biology 2020Quote: ... Sample indices were added using NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set – New England Biolabs). The concentration of final amplicons was measured by Qubit and the length validated by TapeStation HSD1000 prior to sequencing using Illumina MiSeq 600-cycle v3 Reagent Kits with the following run parameters ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 and 2 (NEB #E7335S, #E7500S), following the manufacturer’s instructions with minor modifications ...
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bioRxiv - Neuroscience 2022Quote: ... with NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1) (E7600S, New England BioLabs) based on manufacture instructions ...
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bioRxiv - Genetics 2022Quote: ... using the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England Biolabs, Ipswich, MA). Paired-end 300 bp reads were generated on an Illumina MiSeq.
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bioRxiv - Genetics 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1; NEB, E7335S). Samples were pooled and submitted to MSKCC Integrated Genomics Operation core for quality control and sequencing on Illumina HiSeq 4000 platform.
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bioRxiv - Genetics 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1; NEB, E7335S). Samples were pooled and submitted to MSKCC Integrated Genomics Operation core for quality control and sequencing on Illumina HiSeq 4000 platform.
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bioRxiv - Developmental Biology 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1; NEB, E7335S). Samples were pooled and submitted to MSKCC Integrated Genomics Operation core for quality control and sequencing on Illumina HiSeq 4000 platform.
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bioRxiv - Developmental Biology 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1; NEB, E7335S). Samples were pooled and submitted to MSKCC Integrated Genomics Operation core for quality control and sequencing on Illumina HiSeq 4000 platform.
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bioRxiv - Molecular Biology 2023Quote: ... The samples were multiplexed with NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 and 2) (NEB). Size selection steps were performed with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Evolutionary Biology 2024Quote: ... with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Sets 1 and 2) (NEB #E7600S and #E7780S), with two-sided size selection around a mode of 480 base pairs ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.2 μM primers (primer sequences are presented in Table S1) and 2 × PCR Master mix (M0482S, NEB), with the following cycling parameters ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA was then PCR-amplified using barcoded PCR primers (New England Biolabs #E7335L, #E7500L). After AMPure XP bead purification (Beckman Coulter #A63881 ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was then PCR-amplified using barcoded PCR primers (New England Biolabs #E7335L, #E7500L). After AMPure XP bead purification (Beckman Coulter #A63881 ...
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bioRxiv - Cell Biology 2023Quote: ... Primer extension was catalyzed by 50 units of Protoscript II RT (New England Biolabs, M0368) in a 5 µL reaction containing 0.5 mM dNTP ...
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bioRxiv - Microbiology 2019Quote: ... we performed q-RT PCR using the Luna Universal One-Step RT-qPCR Kit from NEB (E3005), using the Applied BiosystemsΤΜ 7500 Real-Time PCR system ...
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bioRxiv - Genetics 2020Quote: ... and multiplex barcoded with NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2) (NEB, Ipswich, USA). An Illumina MiSeq device (Illumina Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... and samples were barcoded using the NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB), listed in the Supplementary Data 1 ...
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bioRxiv - Genetics 2023Quote: ... Libraries were indexed with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England BioLabs E7600S). Completed libraries underwent quality assessment on the Agilent Fragment Analyzer run by JH SCTC ...
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bioRxiv - Immunology 2022Quote: ... and Luna® Universal RT-PCR Kit (New England Biolabs) in a StepOnePlus apparatus (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... NEB One Taq One-Step RT-PCR Kit (NEB # E5310S) was used for nucleic acid amplification ...
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bioRxiv - Molecular Biology 2019Quote: ... The annealing temperatures for RT-PCR were calculated by NEB Tm Calculator (https://tmcalculator.neb.com/) ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR was carried out using Q5 DNA polymerase (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were set up using the Q5 DNA polymerase (New England Biolabs) according to manufacturer’s instructions and the following primer pairs:
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bioRxiv - Microbiology 2023Quote: ... That primer was used with primer 1092 for PCR with Q5 DNA polymerase with high-GC buffer (NEB). The raw PCR product was used for blunt end ligation as above.
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bioRxiv - Immunology 2022Quote: ... quantitative reverse transcription PCR (qRT-PCR) was conducted using Luna universal probe one-step RT-PCR kit (NEB, #E3006) on CFX96 Touch Real-Time Detection System on 96-well plates (Bio-Rad) ...
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bioRxiv - Bioengineering 2020Quote: ... The RT-PCR detection was conducted by using the Luna Universal One-Step RT-qPCR kit from NEB and following its protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μL of Universal PCR Primer (25 mmol) (NEB, Ipswich, USA), and 1 μL of Index (X ...
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bioRxiv - Microbiology 2022Quote: ... primers for PCR were generated using automated software offered by NEB. pBAD vectors harboring the genes were linearized using the primers in inverse PCR and then incubated in 1X KLD mix provided with the kit for 5-10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... was PCR amplified with mutagenic primer pairs using Q5 polymerase (NEB) (Supplementary Table S1) ...
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bioRxiv - Genomics 2019Quote: ... 1μL SR RT Primer and following the manufacturers protocol (NEB small RNA for Illumina library prep); “Hybridize the reverse transcription primer” followed by “ligate 5’ SR adaptor…” ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA Magnetic Isolation Module and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1/2/3/4) following the manufacturer’s instructions (New England Biolabs). Quantification and quality checked of libraries was done using an Bioanalyzer 2100 instrument and DNA 7500 kit (Agilent Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1 μg cDNA was added to a 50 μl Q5 Hot Start High-Fidelity DNA Polymerase PCR reaction with 0.2 μM each of NEBNext SR Primer for Illumina and NEBNext Index Primer for Illumina (NEB) and run for 6 cycles with a 15 s 62 °C extension step ...
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bioRxiv - Systems Biology 2021Quote: ... the beads were used to amplify libraries using 13 cycles of PCR with the Illumina Forward PE1.0 primer and Illumina Reverse indexed primer (New England Biolabs) in the presence of Kapa HiFi HS DNA Polymerase (Kapa Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription and subsequent PCR amplification was done with OneTaq RT-PCR Kit (New England Biolabs). Primers ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was reverse-transcribed and PCR amplified using Luna Universal One-Step RT-PCR Kit (NEB). SARS-CoV-2 replication was assessed by using primers specific to the N mRNA (Forward 5’-CTCTTGTAGATCTGTTCTCTAAACGAAC-3’ ...
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bioRxiv - Bioengineering 2022Quote: ... 10 μL PCR reactions were set-up using 2 μL 5x HF Buffer (NEB), 0.5 μL 10 mM dNTPs (NEB) ...
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bioRxiv - Plant Biology 2023Quote: ... PCR amplifications were set up using the Q5 protocol (New England BioLabs, MA, USA), with the previously described plasmid (pEE392 ...
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bioRxiv - Microbiology 2020Quote: ... The amplified 1,297 RT-PCR products were digested with MluI (NEB). Amplified DNA products ...
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bioRxiv - Evolutionary Biology 2019Quote: ... RT-PCRs were conducted using Phusion per the manufacturer’s instructions (NEB) for 25 cycles ...