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Citations for New England Biolabs :
351 - 400 of 466 citations for hnRNP K since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... the resin was resuspended in 100 μL of NLS Buffer and 10 μL of Proteinase K (NEB) and the sample was incubated at 50°C for 30 minutes while shaking at 1200 rpm ...
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bioRxiv - Bioengineering 2019Quote: ... the lysate was digested by ∼0.2 mg/ml Proteinase K (New England Biolabs, Cat. # P8102 or P8107S) at 55°C for ∼1 day ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The tissue sections were then lysed in lysis buffer and Protinease K (10mg/ml, New England BioLabs). The lysis buffer contained 50 mM KCL ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: PCR was performed on toe clippings that were incubated overnight at 55° C in tail lysis buffer (10mM Tris pH 8, 0.4M NaCl, 2mM EDTA, 0.1% SDS, 3.6U/mL Proteinase K (NEB)) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Commercial competent cells were used for the K-12 DH10B strain (NEB® 10-beta high efficiency). Transformation of B REL606 and B REL607 was achieved via the chemical competence method ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were quenched by adding SDS to 0.8% (v/v) and 80 mU Proteinase K (NEB P8107S). Cleavage products were diluted 4X in nuclease-free water and subjected to capillary electrophoresis (CE ...
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bioRxiv - Molecular Biology 2020Quote: ... an additional 32 µL of MyK buffer and 8 µL of Proteinase K (NEB, #P8107S, Ispwich, MA) were added to each tube ...
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bioRxiv - Neuroscience 2022Quote: ... Gelated samples were digested in 4 U/ml proteinase K buffer (New England Biolabs, Ipswitch, MA, USA) with 50 mM Tris pH 8.0 (Serva ...
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bioRxiv - Biochemistry 2022Quote: ... mixing with 5 μL of stop buffer (50 mM EDTA and 2 mg/ml Proteinase K (NEB)) ...
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bioRxiv - Genetics 2019Quote: ... at 37°C for 30 minutes and then treated with 140ug of Proteinase K (NEB, Ipswhich, MA) incubating at 55°C in a thermomixer mixing at 900rpm for two hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The beads were drained of all liquids before 2 mg/ml of Proteinase K (New England Biolabs) diluted in PK buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... IP and input samples were incubated with 10 μL of 800 U/mL Proteinase K (NEB P8107S) at 37° for 2 hours ...
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bioRxiv - Genetics 2020Quote: ... 10 mL of a 10% BSA solution was incubated with 2.5 μL of Proteinase K (NEB P8107S) for 30 minutes at room temperature (∼22°C) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA pH 8.0 (Roth) and 1:100 Proteinase K (New England Biolabs 20 mg/ml)) in a 2 ml Eppendorf tube for at least 24 hours at RT in the dark ...
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bioRxiv - Microbiology 2023Quote: ... The lysates (50 μl) were then incubated at 55°C with 1.25 μl proteinase K (NEB, P8107S). After 1h of incubation ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... The ligation products were reverse crosslinked at 65°C overnight with proteinase K (New England Biolabs, P8107S) and DNA was extracted with phenol-chloroform ...
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bioRxiv - Cancer Biology 2023Quote: ... then combined and treated with 1% SDS and 20 µg of proteinase K (New England Biolabs, #P8107S). The reaction was incubated for 1 h at 55°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and the samples were incubated overnight at 47℃ in clearing solution comprised of Protease K (NEB, P8107S) and Clearing Premix (Vizgen ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting eluant was further treated with 1% SDS and 8 U/mL proteinase K (NEB, P8107S) at 42°C for 20 minutes then phenol chloroform extracted and ethanol precipitated.
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bioRxiv - Genomics 2019Quote: ... Oxidized genomic DNA was incubated additional 30 min with 0.8 U of Proteinase K (NEB, Ipswich, MA, USA) at 37°C ...
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bioRxiv - Genomics 2019Quote: ... Glucosylated genomic DNA was incubated additional 30 min with 0.8 U of Proteinase K (NEB, Ipswich, MA, USA) at 37°C ...
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bioRxiv - Genomics 2021Quote: ... phage particles were lysed at 56 °C for 2 h in 550 μL of lysis buffer (100 mM Tris-HCl at pH 8.0, 27.3 mM EDTA, 2% SDS, ~1.6 U Proteinase K [NEB #P8107]). After lysis ...
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bioRxiv - Neuroscience 2021Quote: ... nuclei were resuspended in 200uL digestion buffer with 4 uL protease (an equal volume of protease K (NEB) was substituted if the manufacturer-provided protease was exhausted ...
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bioRxiv - Neuroscience 2019Quote: ... excess gel was removed and embedded specimen were placed in digestion buffer (1X TAE, 0.5% Triton X-100, 0.8 M guanide HCL) with 8 units/ml Proteinase K (NEB) for 2 hours at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... 5 mM EDTA, 200 mM NaCl, 0.2% SDS, supplemented with 0.2 mg/ml Proteinase K from New England Biolabs) at 55°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... and crosslinks were reversed with 10% SDS and 0.6 mg/ml Proteinase K (New England Biolabs, Ipswich, MA) overnight with shaking at 65 °C ...
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bioRxiv - Cell Biology 2023Quote: ... treating each well with 100 μl of lysis buffer (0.45% NP40, 0.45% Tween20, 0.5x NEB Q5 PCR buffer, 0.1 mg/mL NEB Proteinase K) were incubated for 10 min at 55°C in and subsequently heated to 95°C for 10 min to inactivate the proteinase K ...
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bioRxiv - Bioengineering 2022Quote: ... the IP buffer was adjusted to 1% SDS and 0.8 mg Proteinase K (New England Biolabs, Ipswich, MA) was added ...
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bioRxiv - Genomics 2023Quote: ... This was performed with additional RNase (Astral Scientific, Taren Point, Australia) and proteinase K (NEB, Ipswich, MA, USA) treatment following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... cells were harvested 3 days after electroporation and genomic DNA was extracted with 100 μL lysis buffer (10 mM Tris-HCl, pH 7.5, 0.05% SDS, 25 μg/mL proteinase K (NEB)) at 37 °C with incubation for 1 hour ...
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bioRxiv - Genetics 2024Quote: ... then resuspended and incubated in 20uL digestion buffer (1.1mL 1M Tris-HCl of pH6.8 in 50mL deionized water) with 2uL proteinase K (New England Biolabs) at 37°C for 15min ...
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bioRxiv - Molecular Biology 2022Quote: ... The reactions were stopped by adding EDTA and the proteins were digested by adding Proteinase K (New England Biolabs) at 37℃ for 20min ...
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bioRxiv - Molecular Biology 2020Quote: ... The un-crosslinked suspension was treated with 1 μg of RNase A at 37°C for 30 min and with 0.8 units of Proteinase K (NEB) at 45°C for 45 min ...
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bioRxiv - Cell Biology 2022Quote: ... mixed with 10 μl of lysis buffer (10 mM Tris-HCl [pH 8.8], 1 mM EDTA, 25 mM NaCl, 1% SDS and 8U/ml Proteinase K, NEB) for 3 min at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Bound RNA was eluted with 100 μl of Elution Buffer (5mM Tris-HCl, 1mM EDTA and 0.05% SDS) and 1 μl of Proteinase K (New England BioLabs) and incubated for 1.5 hour at 50°C ...
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bioRxiv - Biophysics 2021Quote: Polymerised gels were removed from the coverslip chamber and placed into 6-well plates to undergo digestion in 0.2 mg/ml proteinase K (New England Biolabs) dissolved in digestion buffer (50 mM Tris pH 8.0 (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... samples were incubated in digestion enzyme buffer consisting of digestion buffer supplemented with (0.5%) proteinase K (P8107S, New England Biolabs) and 5% Pronase (11459643001 ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was cut into pieces and PK buffer (100 mM Tris pH 7.4, 50 mM NaCl, 10 mM EDTA) was added together with Proteinase K (NEB). After 90 min of incubation at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: DNA was isolated from single fins by placing them in lysis buffer (10mM Tris pH 8, 100mM NaCl, 10 mM EDTA, 0.5% SDS) with Proteinase K (333µg/mL, NEB P8107S) at 55°C for between four hours and overnight ...
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bioRxiv - Microbiology 2020Quote: ... Beads were resuspended in 48 μL elution buffer (50 mM Tris pH 8, 1 mM EDTA, 1% SDS) and 1.6 U Proteinase K (NEB), incubated at 55 °C for one hour and then 65 °C overnight ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was diluted with one volume of water and treated with 0.5 μL of 20 mg/mL proteinase K (New England Biolabs) at 50°C for 2 hours ...
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bioRxiv - Biochemistry 2019Quote: 5’-Phosphorylated linker (Oligonucleotide K, Supplementary Table 6) was adenylated using a 5’ DNA Adenylation Kit (New England Biolabs) at 20x scale and purified by TRIzol extraction as previously described56 ...
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bioRxiv - Immunology 2019Quote: ... containing 2 µl of 2X lysis buffer (100 mM Tris.HCl, pH 8.0, 100 mM NaCl, 40 µg/ml Proteinase K (New England BioLabs, P8107S), 0.4% SDS ...
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bioRxiv - Genomics 2021Quote: ... DNA was treated with RNase A for 1 hr at 37°C and Proteinase K (New England Biolabs, 8107) for 1 hr at 55°C ...
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bioRxiv - Genetics 2020Quote: ... 200,000 of cells sorted in 400 ul of PBS were treated with 40 μl of proteinase K (NEB, P8107S) and 40 μl of 20% SDS and incubated at 65°C for 16 h in order to reverse the DNA crosslinking ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were treated with 25 µg Proteinase K (1.25 µL of 20 mg/mL stock solution, New England Biolabs) and incubated at 50 °C for 20 minutes ...
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bioRxiv - Genetics 2023Quote: ... The remaining beads were incubated with 1 ul RNasin (Fisher) and 0.5 µl of 20 mg/ml Proteinase K (NEB) at 42°C and 1200 rpm for 15 minutes in a thermomixer ...
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bioRxiv - Molecular Biology 2023Quote: ... that can base pair to the 3’ nucleotide of the cDNA products as described.75,76 The reaction was stopped by adding proteinase K (1 unit; New England Biolabs) and 25 mM EDTA followed by clean-up with a Monarch PCR & DNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... and S2) or EDTA (25 mM final concentration) plus 1.8-units Protease K (all other Figures, New England Biolabs), and incubating at 25°C for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... and TE buffer, DNA was reverse crosslinked by incubating beads in Elution buffer (1% SDS, 0.1M NaHCO3 and Proteinase K (NEB)) ...