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Citations for New England Biolabs :
51 - 100 of 1846 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were incubated with 0.9 mM MnCl2 and 7 U/μl lysate lambda protein phosphatase (λPP; New England BioLabs), or 0.9 mM MnCl2 and H2O as control ...
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bioRxiv - Cell Biology 2021Quote: ... the lysates were treated with endoglycosidase H (NEB).
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bioRxiv - Plant Biology 2021Quote: ... coli lysates were immobilized on amylose resins (NEB), incubated for 1 h at 4 °C with purified GST-XopS ...
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bioRxiv - Molecular Biology 2020Quote: ... lysate was first treated with DNAse I (NEB) to mitigate genomic DNA viscosity when loading into SDS-PAGE gels ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mg of cell lysate was cleared with protein A magnetic beads to remove non-specific interactions (S1425S, NEB) and incubated overnight with anti-ZAP antibody (Proteintech 16820-1-AP ...
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bioRxiv - Cell Biology 2021Quote: Whole cell lysates or eluted proteins after affinity purification were treated with Protein Deglycosylation Mix II (NEB, Cat# P6044), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cell lysates from FLAG-ACBD4(wACBD5_FFAT)/5 (mFFAT) expressing cells were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Cell Biology 2023Quote: ... total proteins isolated from cell lysates were digested with Endoglycosidase H (endo H) enzyme (New England Biolabs, catalog #: P0703L) with G5 reaction buffer at 37°C according to manufacturer’s instruction and the published procedure 15 ...
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bioRxiv - Cell Biology 2023Quote: ... asynchronous or mitotic HeLa cell lysates were incubated with 0.9 mM MnCl2 and 7 U/µl λPP (New England Biolabs) or 0.9 mM MnCl2 and water for 1 h at 30 °C.
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bioRxiv - Microbiology 2021Quote: ... Virus stocks were recovered following linearization of infectious clone plasmids using NotI-HF (NEB), in vitro transcription ...
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bioRxiv - Biophysics 2023Quote: ... The mRNAs were capped using the vaccinia virus capping enzyme kit (New England Biolabs) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... glabrata total cell lysates using the anti-Rad53 antibodies described in the previous section and Protein A magnetic beads (New England Biolabs). The immunoprecipitated samples were run on 8% acrylamide gels ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μg of tissue or cell lysates were treated with or without 2 μL Lambda Protein Phosphatase (LPP) (New England Biolabs) at 30 °C for 15 min (30 min for HEK-293E lysate ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL of cell lysate was used as template for a 25 μL PCR reaction using Taq DNA Polymerase with ThermoPol Buffer (NEB). For each mutant ...
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bioRxiv - Biochemistry 2022Quote: ... 3.75 μl (corresponding to ~7,500 cells) of lysate was used as a template for PCR amplification with Q5 Hot-Start High Fidelity DNA Polymerase (NEB) and unique primer pairs containing an internal locus-specific region and an outer Illumina-compatible adapter sequence ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA was enriched from crude cell lysate in two rounds using biotinylated oligo-dT (IDT Europe) and streptavidin beads (New England Biolabs). RNA isolations were performed in batches in a randomized order ...
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bioRxiv - Molecular Biology 2023Quote: ... was isolated from total RNA or crude cell lysates by 2 rounds of affinity chromatography using oligo d(T)25-derivatized magnetic beads (NEB). RNA yields were quantified by ultraviolet (UV ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: Virus stocks were generated by either transfecting purified in vitro transcribed viral RNA (HiScribe, NEB) or transfecting the infectious cDNA clone containing plasmid in BHK-T7 cells ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was reverse transcribed with Moloney murine leukemia virus (M-MuLV) transcriptase (New England Biolabs). The purity and concentration of RNA were measured using NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... lysates were additionally dephosphorylated using Lambda protein phosphatase (NEB) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were then applied to amylose resins (NEB, E8021) and washed with 20 column volumes of binding buffer supplemented with 14 mM beta-mercaptoethanol ...
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bioRxiv - Genetics 2019Quote: ... the nuclear lysates were digested with 800U KpnI (NEB) overnight at 37°C and 950 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... all Orai1 lysate preparations were treated with PNGase (NEB) to remove all glycosylation ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with amylose resin (New England Biolabs) for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... of extracted RNA (either from whole-cell lysates or sucrose gradient fractions) was performed using the ProtoScript II First Strand cDNA Synthesis Kit (NEB E6560L) according to the manufacturer instructions with some modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... the enzymatic activity of the commerically availablale recombinant vaccinia virus methyltransferase mRNA 2’O-methyltransferase (NEB), abbreviated hereagter as VMTR1 ...
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bioRxiv - Biochemistry 2019Quote: ... the cleared lysate was loaded onto a amylose resin (NEB) and eluted with a maltose gradient ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein/lysate sample de-N-glycosylation using PNGase F (NEB), Endo S (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were flowed over 2 mL amylose resin (NEB) equilibrated with MBP lysis buffer to capture MBP-tagged protein ...
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bioRxiv - Cancer Biology 2022Quote: ... The clarified lysate was added to equilibrated Amylose resin (NEB Cat no ...
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bioRxiv - Microbiology 2019Quote: ... Clarified lysate was added to a chitin resin column (NEB) and washed with Buffer A ...
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bioRxiv - Immunology 2020Quote: ... lysates were digested with endoglycosidase H (endoH) or PNGaseF (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Diluted lysates were treated with Lambda phosphatase (New England BioLabs). For Western analysis ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was incubated with amylose resin (New England Biolabs), and MBP-Ola1p or MBP-Ola1p-monoGFP was eluted in elution buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were incubated with preequilibrated amylose beads (New England BioLabs) for 1 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Clarified lysates were loaded onto NEBExpress Ni spin columns (NEB), washed once with lysis buffer containing 10 mM imidazole ...
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bioRxiv - Cancer Biology 2023Quote: ... lysates were subject to PNGase F treatment (New England Biolabs) to deglycosylate the transporter proteins ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB8_1 (50 mM Tris-HCl pH 8) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB9_1 (50 mM Tris-HCl pH 8) ...
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bioRxiv - Microbiology 2020Quote: ... 25 μg of protein was precipitated from cell lysate as described in Methods and treated with peptide-N-glycosidase F (PNGase F; New England Biolabs, MT, USA) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was extracted from homogenized cell lysate by 4 × 250 µL/brain Oligo (dT)25 magnetic beads (New England Biolabs, Cat: S1419S), and immunoprecipitated (IP ...
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bioRxiv - Cancer Biology 2020Quote: ... cell lysates from three biological replicates for each condition were spotted in nitrocellulose-coated glass slides (Oncyte Avid, Grace-Biolabs, Bend, OR, USA) in technical triplicates ...
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bioRxiv - Cancer Biology 2020Quote: ... cell lysates from three biological replicates for each condition were spotted in nitrocellulose-coated glass slides (Oncyte Avid, Grace-Biolabs, Bend, OR, USA) in technical triplicates using the Aushon 2470 contact printer equipped with 185 μm solid pins ...
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bioRxiv - Physiology 2023Quote: mRNA was extracted from mesenteric and renal artery whole cell lysates using the Monarch Total RNA Miniprep Kit’ (New England Biolabs, Ipswich, Massachusetts, USA). The concentration and purity of the RNA samples were calculated using a NanoDrop 3300 Fluorospectrometer with absorbance set to 260nm and 280nm ...
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bioRxiv - Biophysics 2021Quote: ... The clarified lysate was mixed with amylose resin (New England BioLabs) pre-equilibrated in lysis buffer and incubated for 60 min on a rocking platform ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to amylose resin (New England BioLabs, E8021S). Resin was washed with binding buffer and then eluted with 200 mM maltose in binding buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Cleared lysate was applied onto an amylose resin (New England Biolabs), washed with buffer A (25mM HEPES 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified lysate was applied to chitin resin (New England BioLabs) and allowed to bind at 37°C for 2 h ...