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Citations for New England Biolabs :
251 - 300 of 2534 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... digested in buffer containing 10 mM DTT with Proteinase K (New England Biolabs), and expanded in ultrapure water as for the cell work ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Membranes were incubated overnight at 4 °C with the appropriate antibody: rabbit monoclonal anti-GSK3β (1:1,000; product # 9315, Cell Signalling Technology, New England BioLabs, Whitby, Ontario, Canada), rabbit polyclonal anti-p[Ser9]GSK3β (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies: anti-Glucocorticoid Receptor D6H2L (1:1000 dilution, Cell Signaling, New England BioLabs #12041), anti-PAX7c (1:500 dilution ...
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bioRxiv - Synthetic Biology 2023Quote: ... The expression of each engineered component was validated pre- and post-sort by staining Jurkat cells with an anti-Myc antibody (#2233S, NEB, MA, USA), followed by performing flow cytometry on a FACSCanto (BD Biosciences) ...
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bioRxiv - Plant Biology 2021Quote: ... and anti-MBP antibodies (NEB), respectively.
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibodies (NEB).
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (NEB).
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bioRxiv - Molecular Biology 2020Quote: ... The complexation reaction was carried out in a solution containing 1X NEBuffer 2.1 (NEB) (composed of 100 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... Phosphorylation reaction containing TEX264-FLAG and CK2 (mixture of CK2A1 and CK2B, P6010, NEB) with or without 1 mM CX4549 (S2248 ...
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bioRxiv - Immunology 2021Quote: ... Samples were added to second-strand synthesis mix containing 2× NEB buffer 2 (NEB), 625 nM dNTP Mixture (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... The vector (pPICZαA) containing the AfLPMO16 gene was linearized by Pme1 (New England BioLabs) and transformed to Pichia pastoris X33 competent cells ...
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bioRxiv - Plant Biology 2020Quote: ... 19 μL binding mixture containing 4 μL nuclear extracts and 5U Rnase H (NEB) or bovine pancreatic RNase A (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... in a 10 μL reaction mix containing 1X Luna Universal qPCR Master Mix (NEB) and 400 nM forward and reverse primers ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in 200 μl of MNase reaction buffer containing 2.5U of MNase (NEB, M0247) and incubated for 10 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... plasmid clones containing the correct insert were linearized with DraI (#R0129S, New England Biolabs) and sgRNA was synthesized in vitro using a Maxiscript T7 Transcription Kit (#AM1312 ...
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bioRxiv - Molecular Biology 2021Quote: ... Eighty µl of 1X PNK containing 2µl (20U) of T4 PNK (New England Biolabs), 2µl (80U ...
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bioRxiv - Cell Biology 2019Quote: ... The dUTP-containing strand was degraded using Uracil-DNA Glycosylase (New England Biolabs #M0280S). The resulting single-stranded cDNA was amplified with 13 or 15 PCR cycles followed by size-selection using Pippin Prep (Sage Science) ...
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bioRxiv - Biochemistry 2021Quote: ... Control samples containing only RNA were treated with RNA 5’ Pyrophosphohydrolase (RppH, NEB, M0356S) to convert 5’-PPP RNA into 5’-P RNA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Uracil-containing strands were degraded by treatment with USER Enzyme (New England Biolabs, USA). Sequencing was performed using Illumina Novaseq 6000 Platform in paired-end format with the Phred score of 64 ...
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bioRxiv - Biophysics 2021Quote: ... A plasmid containing two BsaI sites was first treated with BsaI (New England Biolabs), then separated on a 1% agarose gel ...
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bioRxiv - Genomics 2021Quote: ... The sgRNA-containing region was PCR-amplified with NEBNext Ultra II Q5 MasterMix (NEB), acrylamide gel-purified ...
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bioRxiv - Genomics 2020Quote: ... 0.3 uL of TSO digestion buffer containing 1U Uracil-DNA glycosylase (UDG, NEB M0280S) were added to each well ...
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bioRxiv - Genomics 2022Quote: ... A restriction digestion mix containing 7 µl of 10X NEB CutSmart Buffer (NEB #B7204), 4.5 µl of NlaIII (NEB #R0125) ...
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bioRxiv - Genomics 2022Quote: ... Illumina sequencing adapters containing uracil hairpin loop structure and 3′ T overhangs (NEB, #E7337A) were ligated to the DNA by adding 150 units of Quick T4 DNA Ligase (NEB ...
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bioRxiv - Immunology 2023Quote: ... using SYBR green I containing Luna® Universal qPCR Master Mix (NEB England Biolabs). The qPCR was performed using the QuantStudio 5 Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... using SYBR green I containing Luna® Universal qPCR Master Mix (NEB England Biolabs). The qPCR was performed using the QuantStudio 5 Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: ... pDR274 transcription plasmids containing the gRNA sequences were linearized with DraI (NEB; Ipswich, MA) and amplified using Accustart Taq DNA Polymerase HiFi (Quanta Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μL of a mix containing 5 U of T4 RNA Ligase 2 (NEB), 1 mM ATP ...
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bioRxiv - Developmental Biology 2023Quote: ... Injection mixtures containing 500 ng/µl of Cas 9 protein (NEB; Cat. no.: M0641) and 300 ng/µl of guide RNA were prepared and injected into eggs within 4 hours of egg laying ...
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bioRxiv - Biochemistry 2023Quote: ... Annealed oligos containing sgRNA sequences were phosphorylated with T4 polynucleotide kinase (New England Biolabs) and ligated into linearized vector backbone ...
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bioRxiv - Cancer Biology 2023Quote: ... A volume of 40 μl reaction mix containing 5 μl isothermal amplification buffer (NEB), 3 μl 100 mM MgSO4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Annealed oligos containing sgRNA sequences were phosphorylated with T4 polynucleotide kinase (New England Biolabs) and ligated into linearized vector backbone ...
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bioRxiv - Pathology 2023Quote: ... containing 6.25 μL of Taq 2x Master Mix (New England Biolabs, Ipswich, MA, U.S.A), 0.16 μL of the forward primer with the M13 tail ...
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bioRxiv - Molecular Biology 2023Quote: ... in a reaction mix containing 1× T4 RNA Ligase Buffer (New England Biolabs, M0437M), 1U SUPERase•In RNase inhibitor (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... stained in HI buffer containing 5 μM SNAP-Surface Alexa 647 (New England Biolabs) for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: The vector pKGWFS7 containing proSPPiDDR1 was digested by HindIII and MfeI restriction enzymes (NEB) and then loaded on an agarose gel ...
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bioRxiv - Biochemistry 2023Quote: ... The reactions were adjusted to 80 μL containing 1x NEBuffer r1.1 (New England Biolabs) and a final concentration of 1.4 nM of hRNase 4 and 0.15 U/μL of polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2023Quote: ... containing oligo pool template at 3.33 pg/μL and LAMP Fluorescent Dye (B1700, NEB) at 0.5X was aliquoted across a PCR plate ...
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bioRxiv - Molecular Biology 2023Quote: ... pRSSM1.PleII plasmid (containing N4mC at cacCgc sites) (Dr. Iain Murray, NEB Ipswich MA) were used as spike in controls in NGS deamination assay.
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bioRxiv - Genetics 2023Quote: ... containing 1× Exonuclease I Buffer and 1 U/µL Exonuclease I (NEB, cat # B0293S), and incubated at 37 °C for 45 min ...
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bioRxiv - Immunology 2024Quote: Maxi-prepped plasmids containing the replicate barcoded libraries were digested using SalI-HF (NEB) to linearize the plasmid prior to long-read sequencing ...
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bioRxiv - Bioengineering 2024Quote: ... 500 µL PCR reagents containing 1.67X Q5® High-Fidelity Master Mix (NEB, M0515), 0.625 mg/mL BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... Bound antibodies were detected by incubation with DyLight 800-conjugated secondary antibody (New England BioLabs). An InnoScan 710-IR scanner (Innopsys ...
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bioRxiv - Cancer Biology 2024Quote: ... Bound antibodies were detected by incubation with DyLight 800-conjugated secondary antibodies (New England BioLabs), and analysed using an InnoScan 710-IR scanner (Innopsys) ...
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bioRxiv - Biophysics 2021Quote: ... with 10 µl of folding mixture containing 10 nM M13mp18 scaffold DNA (New England Biolabs), 100 nM unmodified oligonucleotides (Integrated DNA technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... agarose plugs containing DNA were washed three times with 1X NEBuffer 3.1 (New England BioLabs) with 5-minute incubations ...
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bioRxiv - Microbiology 2020Quote: ... a single-pot reaction was set up containing T4 ligase (10 000U) and buffer (NEB), SapI (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... Association occurred in samples containing ACE2- fusion proteins either without or with TEV protease (NEB) treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... the dUTP-containing second strand was digested by adding 1 µL of USER enzyme (NEB) and incubating at 37 °C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... MRR1a complementation plasmids containing the correct sequences were linearized with Not1-HF (New England BioLabs), cleaned up with the Zymo DNA Clean & Concentrator kit (Zymo Research ...