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Citations for New England Biolabs :
601 - 650 of 8889 citations for Uric Acid Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... using the Q5-site directed mutagenesis kit (NEB). The 3×HA epitope tag was amplified from the pPR2-HA3 plasmid (Katris et al ...
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bioRxiv - Biochemistry 2024Quote: A Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) was used to introduce mutations in amino acid residues predicted to play a role in stabilizing zinc ions ...
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bioRxiv - Biophysics 2024Quote: ... Gibson Assembly Cloning Kit (NEB, Ipswich, MA, USA) and 5-alpha E ...
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bioRxiv - Genetics 2024Quote: ... with Phusion high-fidelity DNA Polymerase Kit (NEB), Common sgRNA-R and Primer 1 or Primer 2 (see below).
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bioRxiv - Microbiology 2023Quote: ... using the Quick Ligase kit (New England Biolabs), and the ligation mix was used to transform the Escherichia coli M15 strain ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 µg of purified total RNA’s was used for rRNA depletion using Ribo-Zero Gold rRNA Removal Kit (Illumnia, MRZG 12324) or NEBNext rRNA Depletion Kit (NEB, E6310). Library preparation was done according to the manufacturer’s instructions with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The library of each sample was prepared separately with the NEBNext® Ultra (tm) II FS DNA Library Preparation Kit and the Unique Dual Indexing kit (New England Biolabs), with reagent volumes reduced to one-fifteenth of that advised by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... was then gel purified using the Qiagen MinElute Gel Extraction Kit (28604) and subjected to Gibson assembly via the New England Biosciences HiFi DNA Assembly Cloning Kit (NEB: E5520S) to insert the amplified region into the hSyn-eGFP vector following its linearization with BamHI (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were qualified on Agilent 2100 Bioanalyzer using High Sensitivity DNA kit and quantified by qPCR using NEBNext Library Quant kit for Illumina (NEB, E7630). Equal molarity of each library were pooled and subjected to sequencing on Illumina HiSeq 4000 and NovaSeq 6000 platforms at the McGill University and Génome Québec Innovation Centre to generate 50 bp paired-end reads.
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bioRxiv - Pathology 2021Quote: ... The kit used for library construction was the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Total RNA extracted from the samples was subject to mRNA enrichment using poly-T oligo-attached magnetic beads ...
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bioRxiv - Genomics 2021Quote: ... We individually indexed samples to be pooled within the same sequencing lane during the PCR enrichment step using NEBNext® Multiplex Oligos for Illumina® (single-indexed with E7335 and E7500 kits, or dual-indexed with E6440 kit, New England Biolabs). We performed in-solution hybridization and target enrichment using our 12,049 tiled RNA probes ...
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bioRxiv - Genetics 2021Quote: ... Vector components were purified using the Monarch® DNA Gel Extraction Kit or the Monarch® PCR & DNA Cleanup Kit (NEB) and assemblies were performed using NEBuilder® HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: RNA-seq libraries for RIP-TRIBE and polysome were prepared in triplicates from 150 ng of total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with the NEBnext rRNA depletion Kit (NEB #E6310L). cDNA libraries were sequenced on an Illumina NovaSeq6000 using S-prime or S1 flow cells with paired-end 150-bp reads ...
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bioRxiv - Biochemistry 2022Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s instructions (New England Biolabs, MA, USA). Sequencing experiments were performed with NextSeq 500 (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s instructions (New England Biolabs, MA, USA). Sequencing experiments were performed using NextSeq 500 (Illumina ...
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bioRxiv - Genomics 2022Quote: We constructed NEBNext libraries with 200- and 10-ng RNA by following the guideline of NEBNext Ultra II RNA Library Prep Kit for Illumina kit (NEB, E7770). SmartSeq2 libraries with single cells were prepared following the protocol that was reported by Picelli ...
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bioRxiv - Molecular Biology 2022Quote: ... Library quality was checked by Agilent BioAnalyzer 2100 using the High Sensitivity DNA kit (Part number 5067-4626) and libraries were quantified using the Library Quant Kit for Illumina (NEB #7630). Libraries were then sequenced using a NextSeq500 platform (75-base-pair (bp ...
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bioRxiv - Molecular Biology 2022Quote: ... Library quality was checked by Agilent BioAnalyzer 2100 using the DNA 1000 kit (Part number 5067-1504) and libraries were quantified using the Library Quant Kit for Illumina (NEB #7630). Libraries were then sequenced using a NextSeq500 platform (75-base-pair (bp ...
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bioRxiv - Biochemistry 2023Quote: ... loci were amplified using the NGSgo®-MX6-1 kit (GenDx) and multiplexed sequencing libraries were prepared using the Ultra™ II FS DNA Library Prep Kit for Illumina (NEB). Libraries were sequenced on a NovaSeq 6000 system (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription into cDNA was performed using a cDNA synthesis kit (ProtoScript First Strand cDNA Synthesis Kit, New England BioLabs, Ipswich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... These amplicons were used as templates for in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap® Reagent AG kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s protocols (New England Biolabs, MA, USA), as described previously58 ...
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bioRxiv - Molecular Biology 2023Quote: Libraries were qualified on Agilent 2100 Bioanalyzer using High Sensitivity DNA Kit and quantified by qPCR using NEBNext Library Quant Kit for Illumina (NEB, E7630). Equal molarity of each library was pooled and subjected to sequencing on Illumina NovaSeq 6000 platform at the McGill University and Génome Québec Innovation Centre to generate 100 bp paired-end reads.
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bioRxiv - Biophysics 2023Quote: ... Vector DNA was linearized with NheI or MluI and cRNA synthesized in vitro using the SP6 or T7 AmpliCap Max High Yield Message Maker Kit (Cellscript, USA) or HiScribe® T7 ARCA mRNA Kit (New England Biolabs) and stored at −20°C (for frequent use ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized with RevertAid First Strand cDNA Synthesis Kit (ThermoScientific) reverse transcription kit and PCR was carried out using OneTaq® DNA Polymerase reagents (NEB) using conditions previously detailed and oligonucleotides described in Suppl ...
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bioRxiv - Cell Biology 2024Quote: ... samples were run on a 1% agarose gel and areas corresponding to the expected size were excised and purified from the gel using a gel extraction kit (Monarch DNA Gel Extraction Kit, NEB, USA). This PCR product was used for a second round of amplification followed by gel purification ...
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bioRxiv - Bioengineering 2022Quote: ... and a sequence spanning the TRAC target site was PCR-amplified from cell lysates to be used to determine the level of genomic disruption of TRAC by a T7E1 Surveyor Nuclease assay (NEB, Ipswich, MA, United States). The PCR primers used for the amplification of the target locus were as follows ...
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bioRxiv - Molecular Biology 2020Quote: Individual LAMP assays were assembled in a total volume of 25 μl of 1X Isothermal buffer (NEB; 20 mM Tris-HCl, 10 mM (NH4)2SO4 ...
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bioRxiv - Developmental Biology 2020Quote: A Fragment Analyzer™ Automated CE System (Analysis Kit: cat.: DNF-474-0500) was used to assess the fragment distributions and a Library Quant Kit for Illumina (NEB, cat.: E7630L) was used to measure molar concentrations ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg/ml glycogen) and DNA purification was carried out with the PCR cleanup kit (NEB, Monarch PCR & DNA Cleanup Kit, T1030). About 10ng of the purified CUT&RUN DNA was used for preparation of multiplexed libraries with the NEB Ultra II DNA Library Prep Kit per manufacturer’s instruction (NEB #E7103) ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-seq libraries were synthesized using an NEB Next Ultra RNA Library Prep Kit for Illumina and an NEB Next Adaptor for Illumina Kit (New England Biolabs, MA, USA). Paired-end sequencing was performed using the HiSeqXten (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA sequencing libraries were prepared from 100 ng of input RNA using the non-directional kit NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB, #E7775) with the NEBNext Poly(A ...
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bioRxiv - Microbiology 2022Quote: Site directed mutagenesis was carried out using Q5® Site-Directed Mutagenesis Kit using manufacturer’s protocol (source of kit, NEB or else) using primers carrying the altered codon were designed using the online tool OligoCalc ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were prepared according to the instructions of the Kit “NEBNext Ultra Directional RNA Library Prep kit for Illumina” (E7760L, New England Biolabs, Ipswich, MA), following the protocol “Poly(A ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated using a genomic DNA extraction kit (Monarch Genomic DNA Purification Kit, New England Biolabs, Frankfurt am Main, Germany) and the PCR was optimized to yield a single amplicon ...
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bioRxiv - Cancer Biology 2023Quote: ... library was prepared using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). The RNA-Seq libraries were sequenced on NextSeq 500 using the NextSeq 500/550 High Output Kit v2.5 (75 cycles ...
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bioRxiv - Immunology 2023Quote: The total RNA of macrophage groups was isolated with a Nucleo-Spin RNA kit (Macharey Nagel, Germany) or Monarch Total RNA Miniprep Kit (New England Biolabs, Massachusetts, ABD). The purity and quantity of RNA were evaluated by a Nanodrop spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Stranded RNA-seq libraries were constructed using NEBNext poly(A) mRNA isolation kit along with the SWIFT RNA Library Kit (NEB, MA, USA) according to the established protocols at Laboratory for Molecular Biology and Cytometry Research (LMBCR ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was purified using the QIAquick PCR purification kit and it was prepared for Illumina sequencing using the NEBNext Ultra II A Library Prep Kit (New England Biolabs; Cat# E7645L).
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bioRxiv - Plant Biology 2020Quote: ... respectively by Phusion® High-Fidelity PCR Kit (NEB) (Harashima & Schnittger ...
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bioRxiv - Plant Biology 2019Quote: ... and the Luna One-Step RT-qPCR Kit (NEB) in accordance with the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... After purified with Monarch RNA Cleanup Kit (NEB Biolabs), RNAs were polyadenylated with E ...
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bioRxiv - Cancer Biology 2021Quote: ... After purified with Monarch RNA Cleanup Kit (NEB Biolabs), RNAs were polyadenylated with E ...
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bioRxiv - Molecular Biology 2020Quote: ... and qPCR analysis by NEB Next Library Quant Kit for Illumina (New England Biolabs). All sequencing was performed using an Illumina MiSeq (Illumina ...
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bioRxiv - Cell Biology 2020Quote: Libraries were prepared using Next Ultra II kits (NEB) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Using the NEBuilder HiFi DNA assembly cloning kit (NEB), EBOV VP30 was inserted into a pLenti6.3 vector (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: PCR fragments were assembled using Gibson assembly kit (NEB) and verified by Sanger sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... and reverse transcribed using Lunascript kit (New England Biolabs). qPCR was performed on a Light Cycler 480 (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... purified with the Monarch PCR & DNA Cleanup Kit (NEB) and ligated with T4 DNA ligase (NEB) ...
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bioRxiv - Developmental Biology 2021Quote: ... Using Q5® Site-Directed Mutagenesis Kit (NEB, E0554), we generated mutations in each of the two most highly conserved Rbpj binding sites (m1 and m2 ...