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Citations for New England Biolabs :
1 - 50 of 3150 citations for Trypsin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and tryptic peptides were generated using Trypsin-Ultra (P8101S, NEB) following the protocol described previously2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then 0.3 μL of 1 mg mL−1 trypsin (trypsin-ultra, MS-grade, New England Biolabs) was added and samples were incubated at 37 °C overnight ...
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bioRxiv - Genomics 2023Quote: ... Blocking was achieved using 1% BSA (NEB) and 0.1% Tween-20 in 1× PBS for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin digestion was initiated by adding 1:10 (trypsin to protein, m/m) mass spectrometry grade trypsin (New England Biolabs #P8101S) in 50 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2024Quote: ... The peptides obtained by trypsin digestion were subsequently labeled as directed by the tandem mass tag (TMT) kit (PTM Biolabs). The labeling peptides were then fractionated by high pH reverse-phase high-performance liquid chromatography and further subjected to liquid chromatography with tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Cell Biology 2020Quote: ... Trypsin (NEB) (cuts after Lys/Arg ...
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bioRxiv - Microbiology 2021Quote: ... The protein was pelleted at 18,000xg for 10 minutes and supernatant was removed before the pellet was resuspended in 50 μL trypsin reaction buffer and 1 μg trypsin (New England Biolabs) added and the suspension incubated overnight at 37°C ...
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bioRxiv - Genomics 2022Quote: ... 500 nL of 5hmC-blocking mix (1 U T4-BGT (NEB, M0357L), 6x UDP-glucose ...
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bioRxiv - Biochemistry 2023Quote: ... Desalted peptides were dissolved in 1 ml 1 × CutSmart buffer (pH 8, New England BioLabs) and incubated with 50 units of alkaline phosphatase (calf intestinal ...
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bioRxiv - Biophysics 2022Quote: ... 2 μL of a 0.4 µg µL-1 stock of Trypsin (NEB) were added to the samples (to a final enzyme:substrate ratio of 1:50 w/w ...
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bioRxiv - Biochemistry 2023Quote: ... In-gel digestion was performed with trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega, Madison, WI; Trypsin-ultraTM, MS grade NEB) with a final trypsin concentration of 20 ng/µl in 50 mM aqueous ammonium bicarbonate and 5 mM CaCl2 ...
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bioRxiv - Cell Biology 2021Quote: Trypsin (Promega or NEB) was added to the histone sample at a 1:20 ratio (e.g. ...
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bioRxiv - Biophysics 2020Quote: ... Trypsin-ultra™ (NEB Inc.) was added to all samples at final ratio of 1:50 by mass and the samples were digested for 30 min at 37 °C ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin (0.15 ng) (New England Biolabs) was added following the removal of an aliquot at the zero-time point ...
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bioRxiv - Molecular Biology 2023Quote: ... Three peptide phage libraries from NEB “New England Biolabs” ...
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bioRxiv - Biochemistry 2022Quote: ... PNGase F (P0704) and mass spectrometry grade Trypsin (Trypsin-Ultra, P8101) are from New England Biolabs (NEB). Fbs1-GYR proteins were expressed and purified from E ...
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bioRxiv - Microbiology 2022Quote: ... 10 min) and resuspended in 50 µl of 50 mM Tris-HCl containing 1 µg trypsin (NEB). After overnight incubation at 37 °C ...
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bioRxiv - Biophysics 2019Quote: ... the imaging chamber was incubated in blocking buffer (10 mM Tris HCl pH 7.5, 50 mM NaCl, 1 mg/mL BSA [NEB], 1 mg/mL tRNA [Ambion]) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... Peptide-N-Glycosidase F (PNGase F; NEB, P0704) treatment of lysates was performed as described in the manufacturer’s directions ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 1 mM MnCl2 was used to remove the phosphorylation on the peptides and Staurosporin (NEB, Hitchin, UK) to occupy the active site pocket ...
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bioRxiv - Genetics 2022Quote: ... and blocking oligo wash (0.25X T4 RNA ligase buffer (NEB), 0.3uM blocking oligo ...
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bioRxiv - Cancer Biology 2022Quote: ... and blocking oligo wash (0.25X T4 RNA ligase buffer (NEB), 0.3 µM blocking oligo ...
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bioRxiv - Molecular Biology 2021Quote: ... and blocking oligo wash (0.25X T4 RNA ligase buffer (NEB), 0.3uM blocking oligo ...
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bioRxiv - Microbiology 2023Quote: ... and the prototype serine endopeptidase trypsin (New England BioLabs), placed within a fluorescence plate reader (Spectra max Gemini XPS ...
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bioRxiv - Microbiology 2021Quote: ... Peptide-N-glycosidase F (PNGase F) (New England BioLabs) was used to remove all N-linked oligosaccharides for 1 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... or for peptide N-glycosidase F (PNGase-F, NEB) digestion using 500 units PNGase-F for 1,5h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... the tissue samples were stained using Vizgen’s Cell Boundary Kit (Vizgen, 10400009) and blocked in blocking solution (Vizgen, 20300012) supplemented with a 1:20 dilution of Rnase inhibitor (NEB, M0314L) for one hour ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were heated for 1 minute at 100°C followed by the addition of 2 μL of peptide N-glycosidase F (New England Biolabs) to release the N-glycans ...
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bioRxiv - Biophysics 2022Quote: ... Total of 60 ug of trypsin (mass spectrometry grade, NEB) was diluted in 25 mM NH4HCO3 and added to dried gel pieces (3x volume of the gel volume) ...
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bioRxiv - Bioengineering 2019Quote: ... 7-mer random peptide phage library (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Bioengineering 2019Quote: ... 7-mer random peptide phage library (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2022Quote: ... or peptide N-glycanase (PNGase, New England Biolabs, 1,000 units) for 6 h ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μL of peptide N-glycosidase F (New England Biolabs) were added ...
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bioRxiv - Neuroscience 2021Quote: ... with 1% (w/v) octylglucoside containing 5 U endoglycosidase H (Endo H) or peptide: N-glycosidase F (PNGase F; both NEB, Frankfurt, Germany) at 37°C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... GST-IpaH4-3XFlag was cloned into pGEX6P-1 with a 3XFlag peptide followed by the coding sequence for ubiquitin using Gibson Cloning (NEB; Table S4). Sequence-verified constructs were transformed into E ...
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bioRxiv - Immunology 2020Quote: ... The peptides were first digested with Endo H (New England Biolabs) to deplete oligomannose- and hybrid-type glycans and leave a single GlcNAc residue at the corresponding site ...
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bioRxiv - Bioengineering 2020Quote: ... reesei strain by peptide N-glycosidase F (PNGase F, NEB, P0704) (Wang et al ...
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bioRxiv - Cell Biology 2022Quote: ... or Peptide-N-Glycosidase F (PNGase F) (New England Biolabs, #P0704S) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... and Ph.D.-C7 peptide library (New England Biolabs, Ipswich, MA, USA) for the identification of AbOmpA binding phage ...
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bioRxiv - Biochemistry 2021Quote: Peptide-N-glycosidase F (PNGase F) (New England Biolabs Inc., Cat. P0704S) was used for complete removal of N-linked oligosaccharides ...
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bioRxiv - Plant Biology 2020Quote: ... 1.5 μg of biotinylated histone peptides were incubated with streptavidin beads (NEB) in binding buffer (50 mM Tris-HCl 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... separated via self-cleaving T2A peptide was generated via Gibson Assembly (NEB). This cassette including a 5’ chimeric intron and 3’ poly adenylation signal sequence replaces the sequence of Pax7 exon 1 immediately 3’ of the ATG start codon ...
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bioRxiv - Developmental Biology 2023Quote: ... Trypsin digestion was stopped by adding 5% BSA (ref. B9000S, New England Biolabs) and the cell suspension was passed through a 40 µm Flowmi cell strainer (ref ...
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bioRxiv - Immunology 2022Quote: ... N-glycans were enzymatically removed from the underlying peptides with PNGase F (NEB) overnight at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... A 7-mer random peptide library (E8100S, Ph.D. -7, New England Biolabs, USA) was panned overnight at 4°C on pooled human IgM adsorbed on polystyrene plates at a concentration of 0.1 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The Peptide-N-Glycosidase F (PNGase F) enzyme (New England Biolabs, catalog #: P0704L)-treated samples served as a control for unglycosylated α1 subunits ...
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bioRxiv - Immunology 2023Quote: ... N-glycans were enzymatically removed from the tryptic peptides with PNGase F (NEB) overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 250 μL of sample with 16 μg/mL TPCK-treated trypsin (New England BioLabs), 2X penicillin and streptomycin and 2X antibiotic-antimycotic (Wisent ...
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bioRxiv - Evolutionary Biology 2020Quote: Phage display experiments were performed using the PhD-12 peptide phage display kit (NEB). All steps involving the pipetting of phage-containing samples was done using filter tips (Rainin) ...
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bioRxiv - Biochemistry 2020Quote: ... tsetse saliva was treated with peptide-N-glycosidase A (PNGase A, New England Biolabs), which releases all N-linked glycans ...