Labshake search
Citations for New England Biolabs :
351 - 400 of 1715 citations for Tripartite Motif Containing Protein 55 TRIM55 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... and protein samples and molecular weight markers (PageRuler Plus Pre-stained Protein Ladder, ThermoFisher Scientific 26619, or Colour pre-stained protein standards NEB P7712 or P7719) were separated using 8% ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 % SDS-PAGE gel was prepared and 50 μg of protein lysate was loaded along with the protein standard ladder (Cat.No.: P7719S, New England Biolabs, Ipswich, Massachusetts, United States), and electrophoresis was performed for 1.5 hr in ice-cold 1x Laemmli electrophoresis running buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Analytical amounts of twenty Argonaute proteins were synthesized from pET29a plasmids using PURExpress In Vitro Protein Synthesis kit (New England Biolabs, Inc., Ipswich, MA, USA). For large scale expression and purification of CbAgo ...
-
bioRxiv - Plant Biology 2021Quote: ... and anti-MBP antibodies (NEB), respectively.
-
bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibodies (NEB).
-
bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (NEB).
-
bioRxiv - Molecular Biology 2020Quote: ... The complexation reaction was carried out in a solution containing 1X NEBuffer 2.1 (NEB) (composed of 100 mM NaCl ...
-
bioRxiv - Cell Biology 2022Quote: ... Phosphorylation reaction containing TEX264-FLAG and CK2 (mixture of CK2A1 and CK2B, P6010, NEB) with or without 1 mM CX4549 (S2248 ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were stained with DMEM containing 1 µM SNAP-Cell SiR (New England Biolabs) and optionally 0.1 % (v/v ...
-
bioRxiv - Immunology 2021Quote: ... Samples were added to second-strand synthesis mix containing 2× NEB buffer 2 (NEB), 625 nM dNTP Mixture (NEB) ...
-
bioRxiv - Biochemistry 2021Quote: ... The vector (pPICZαA) containing the AfLPMO16 gene was linearized by Pme1 (New England BioLabs) and transformed to Pichia pastoris X33 competent cells ...
-
bioRxiv - Plant Biology 2020Quote: ... 19 μL binding mixture containing 4 μL nuclear extracts and 5U Rnase H (NEB) or bovine pancreatic RNase A (Qiagen ...
-
bioRxiv - Cell Biology 2020Quote: ... in a 10 μL reaction mix containing 1X Luna Universal qPCR Master Mix (NEB) and 400 nM forward and reverse primers ...
-
bioRxiv - Cell Biology 2022Quote: ... resuspended in 200 μl of MNase reaction buffer containing 2.5U of MNase (NEB, M0247) and incubated for 10 min at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... plasmid clones containing the correct insert were linearized with DraI (#R0129S, New England Biolabs) and sgRNA was synthesized in vitro using a Maxiscript T7 Transcription Kit (#AM1312 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Eighty µl of 1X PNK containing 2µl (20U) of T4 PNK (New England Biolabs), 2µl (80U ...
-
bioRxiv - Cell Biology 2019Quote: ... The dUTP-containing strand was degraded using Uracil-DNA Glycosylase (New England Biolabs #M0280S). The resulting single-stranded cDNA was amplified with 13 or 15 PCR cycles followed by size-selection using Pippin Prep (Sage Science) ...
-
bioRxiv - Biochemistry 2021Quote: ... Control samples containing only RNA were treated with RNA 5’ Pyrophosphohydrolase (RppH, NEB, M0356S) to convert 5’-PPP RNA into 5’-P RNA ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Uracil-containing strands were degraded by treatment with USER Enzyme (New England Biolabs, USA). Sequencing was performed using Illumina Novaseq 6000 Platform in paired-end format with the Phred score of 64 ...
-
bioRxiv - Biophysics 2021Quote: ... A plasmid containing two BsaI sites was first treated with BsaI (New England Biolabs), then separated on a 1% agarose gel ...
-
bioRxiv - Genomics 2021Quote: ... The sgRNA-containing region was PCR-amplified with NEBNext Ultra II Q5 MasterMix (NEB), acrylamide gel-purified ...
-
bioRxiv - Genomics 2020Quote: ... 0.3 uL of TSO digestion buffer containing 1U Uracil-DNA glycosylase (UDG, NEB M0280S) were added to each well ...
-
bioRxiv - Genomics 2022Quote: ... A restriction digestion mix containing 7 µl of 10X NEB CutSmart Buffer (NEB #B7204), 4.5 µl of NlaIII (NEB #R0125) ...
-
bioRxiv - Genomics 2022Quote: ... Illumina sequencing adapters containing uracil hairpin loop structure and 3′ T overhangs (NEB, #E7337A) were ligated to the DNA by adding 150 units of Quick T4 DNA Ligase (NEB ...
-
bioRxiv - Immunology 2023Quote: ... using SYBR green I containing Luna® Universal qPCR Master Mix (NEB England Biolabs). The qPCR was performed using the QuantStudio 5 Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific) ...
-
bioRxiv - Genomics 2022Quote: ... pDR274 transcription plasmids containing the gRNA sequences were linearized with DraI (NEB; Ipswich, MA) and amplified using Accustart Taq DNA Polymerase HiFi (Quanta Biosciences ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 μL of a mix containing 5 U of T4 RNA Ligase 2 (NEB), 1 mM ATP ...
-
bioRxiv - Biochemistry 2023Quote: ... containing a His6-tagged Ulp1 gene was transformed into T7 Express competent cells (NEB) and grown in LB medium supplemented with ampicillin (100 μg/mL ...
-
bioRxiv - Immunology 2023Quote: ... using SYBR green I containing Luna® Universal qPCR Master Mix (NEB England Biolabs). The qPCR was performed using the QuantStudio 5 Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... containing 5 µl of collection buffer (NEBNext Single Cell Lysis Module, New England Biolabs). Noteworthy ...
-
bioRxiv - Biochemistry 2023Quote: ... Annealed oligos containing sgRNA sequences were phosphorylated with T4 polynucleotide kinase (New England Biolabs) and ligated into linearized vector backbone ...
-
bioRxiv - Cancer Biology 2023Quote: ... A volume of 40 μl reaction mix containing 5 μl isothermal amplification buffer (NEB), 3 μl 100 mM MgSO4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... in a reaction mix containing 1× T4 RNA Ligase Buffer (New England Biolabs, M0437M), 1U SUPERase•In RNase inhibitor (Invitrogen ...
-
bioRxiv - Pathology 2023Quote: ... containing 6.25 μL of Taq 2x Master Mix (New England Biolabs, Ipswich, MA, U.S.A), 0.16 μL of the forward primer with the M13 tail ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Annealed oligos containing sgRNA sequences were phosphorylated with T4 polynucleotide kinase (New England Biolabs) and ligated into linearized vector backbone ...
-
bioRxiv - Genetics 2023Quote: ... containing 1× Exonuclease I Buffer and 1 U/µL Exonuclease I (NEB, cat # B0293S), and incubated at 37 °C for 45 min ...
-
bioRxiv - Molecular Biology 2023Quote: The vector pKGWFS7 containing proSPPiDDR1 was digested by HindIII and MfeI restriction enzymes (NEB) and then loaded on an agarose gel ...
-
bioRxiv - Biochemistry 2023Quote: ... The reactions were adjusted to 80 μL containing 1x NEBuffer r1.1 (New England Biolabs) and a final concentration of 1.4 nM of hRNase 4 and 0.15 U/μL of polynucleotide kinase (New England Biolabs) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... containing oligo pool template at 3.33 pg/μL and LAMP Fluorescent Dye (B1700, NEB) at 0.5X was aliquoted across a PCR plate ...
-
bioRxiv - Immunology 2024Quote: Maxi-prepped plasmids containing the replicate barcoded libraries were digested using SalI-HF (NEB) to linearize the plasmid prior to long-read sequencing ...
-
bioRxiv - Molecular Biology 2023Quote: ... pRSSM1.PleII plasmid (containing N4mC at cacCgc sites) (Dr. Iain Murray, NEB Ipswich MA) were used as spike in controls in NGS deamination assay.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... stained in HI buffer containing 5 μM SNAP-Surface Alexa 647 (New England Biolabs) for 15 minutes at room temperature ...
-
bioRxiv - Bioengineering 2024Quote: ... 500 µL PCR reagents containing 1.67X Q5® High-Fidelity Master Mix (NEB, M0515), 0.625 mg/mL BSA ...
-
bioRxiv - Biophysics 2021Quote: ... The E1371Q samples were phosphorylated using protein kinase A (NEB). The wild-type sample was de-phosphorylated using λ-phosphatase ...
-
bioRxiv - Cell Biology 2020Quote: ... 5 µl 10x buffer for Protein MetalloPhosphatases (New England Biolabs), and 5 µl 10 mM MnCl2 for 30 min at 30 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins associated to chromatin (pellet) were treated with DNaseI (NEB). Finally ...
-
bioRxiv - Molecular Biology 2020Quote: ... both proteins were deglycosylated by PNGase F (NEB, 1:50) overnight at 37°C in PBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... A slurry of protein A or G magnetic beads (NEB) was used to capture enriched chromatin ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were digested using 4µl Proteinase K (800U/ml, NEB) and incubation at 55°C for 2h ...
-
bioRxiv - Synthetic Biology 2019Quote: The PURExpress in vitro protein synthesis kit (New England BioLabs) was used to transcribe and translate Were-1-Fluc ...