Labshake search
Citations for New England Biolabs :
1 - 50 of 10000+ citations for Triiodothyronine T3 ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2019Quote: ... 1× T3 ligation buffer (NEB) and 300 ng total RNA with or without FTO treatment ...
-
bioRxiv - Genetics 2020Quote: ... and T3 (5’-AATTAACCCTCACTAAAGGG-3’) promoter-tagged PCR fragment from each gene using corresponding T7 and T3 RNA polymerase (T3:M0378S; T7:M0251S, BioLabs). Primers used for PCR are listed in Supplemental table 1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and T3 RNA polymerase (New England Biolabs Inc.) with dfr full-length cDNA in pBSKS (gift from W ...
-
bioRxiv - Developmental Biology 2022Quote: ... T3 or SP6 RNA polymerase (New England Biolabs) and digRNA labelling mix (Roche) ...
-
bioRxiv - Plant Biology 2021Quote: ... EcoRI/T7 RNA polymerase and HindIII/T3 RNA polymerase (NEB) generated antisense and sense probes ...
-
bioRxiv - Developmental Biology 2020Quote: ... and T3 or SP6 RNA polymerase (New England Biolabs Inc.). Slides were permeabilized in 0.1% Triton-X100/PBS for 30min and blocked in acetylation buffer ...
-
bioRxiv - Microbiology 2023Quote: ... All mutations in pBAD T3 SAMase were introduced using Q5 Site-Directed Mutagenesis Kit or KLD Master Mix (NEB). Plasmids were transformed into chemically competent XL1-Blue cells (Evrogen) ...
-
bioRxiv - Cell Biology 2019Quote: ... 4µl (or 200Units) of T3 (or T7) RNA polymerase (NEB cat. M0378S) added and incubated at 37°C overnight ...
-
bioRxiv - Genetics 2021Quote: ... mRNA was generated from the linearized plasmids with the T3 RNA polymerase (NEB). 200 pg of wild-type or mutant pola2 mRNA were injected into embryos from a cross at the 1-cell stage ...
-
bioRxiv - Genetics 2019Quote: ... The ligation reaction mixture B consisted of appropriate amount of T3 ligase (NEB) with ligation buffer ...
-
bioRxiv - Plant Biology 2021Quote: ... riboprobes were prepared by invitro transcription using T3 and T7 RNA polymerases (NEB), respectively ...
-
bioRxiv - Developmental Biology 2022Quote: ... with SP6 and T3 primers and Phusion High Fidelity DNA polymerase (New England, BioLabs). The purified product was used as a template for the transcription reaction using the mMessage mMachine SP6 RNA transcription kit (AM1340 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin (DIG)-labeled RNA probes were synthesized using T7 or T3 RNA polymerases (NEB, USA) with the DIG RNA Labeling Mix (Roche ...
-
bioRxiv - Physiology 2019Quote: ... In vivo transcription was carried out using the RNA polymerase SP6/T3 (#M0378S/M0207S, NEB) followed by template DNA degradation using DNAseI (#M0303S ...
-
bioRxiv - Synthetic Biology 2023Quote: All ligases (T3, T4, E.coli, T7, Taq) used during this work were obtained from NEB. Ligations were performed as indicated by the supplier ...
-
bioRxiv - Physiology 2020Quote: ... and T7 polymerase (50 U/μl) or T3 polymerase (50 U/μl) (NEB, Hitchin, Hertfordshire, UK) with 1 μg of linearised vector containing the ArCCKP cDNA ...
-
bioRxiv - Neuroscience 2023Quote: ... Antisense RNAs were synthesized from linearized plasmids using T3 RNA polymerase (New England Biolabs, Ipswich, MA), [α-35S]-CTP ...
-
bioRxiv - Developmental Biology 2023Quote: ... The PCR products were used as templates for transcription reactions using T3 RNA polymerase (Biolabs, M0378S) or T7 RNA polymerase (TOYOBO ...
-
bioRxiv - Developmental Biology 2023Quote: Antisense riboprobes were transcribed using NEB T7 or T3 RNA polymerase (NEB #M0251s and Fisher #501047499) and labeled with digoxygenin (DIG ...
-
bioRxiv - Developmental Biology 2024Quote: Antisense riboprobes were transcribed using NEB T7 or T3 RNA polymerase (NEB #M0251s and Fisher Science #501047499) and labeled with digoxygenin (DIG ...
-
bioRxiv - Neuroscience 2024Quote: Blood corticosterone levels were measured using the Mouse corticosterone Enzyme-linked immunosorbent assay (ELISA) kit (BIOLABS, USA) by collecting blood from wild-type mice ...
-
bioRxiv - Genomics 2020Quote: ... the resulting synthetic RNA/DNA hybrid fragment was ligated to both the 600 and 400 bp DNA fragments before ligating a loop (PS359) and Y-shape (triple-biotin/PS866) at either end using T3 DNA ligase (NEB). The hairpins were gel purified and attached to paramagnetic MyOne T1 streptavidin beads (Dynal/Life Tech).
-
bioRxiv - Developmental Biology 2021Quote: ... RNA probes for Popi-Dfd were synthetized from the plasmid template of Popi-Dfd3’ using T7/T3 RNA polymerase (New England Biolabs, MA, USA).
-
bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
-
bioRxiv - Genomics 2021Quote: ... into 96-well plate containing 2.5μl of methylase reaction buffer (1 × M.CviPI Reaction buffer (NEB), 2 U M.CviPI (NEB) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 μl 10 μM Illumina barcode (NEB-kit) to 23 μl of cDNA ...
-
bioRxiv - Developmental Biology 2019Quote: ... E7.5) into 96 well PCR plates containing 2.5μl of methylase reaction buffer (1× M.CviPI Reaction buffer (NEB), 2U M.CviPI (NEB) ...
-
bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 N-specific primers (EV Table 1 ...
-
BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... PCR was carried out in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 N-specific primers (Forward 5′-TAA TCA GAC AAG GAA CTG ATT A-3′ ...
-
BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... were amplified in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a QuantStudio 6 Flex thermocycler ...
-
bioRxiv - Microbiology 2021Quote: ... PCR was carried out in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 NP-specific primers (Forward 5’-TAA TCA GAC AAG GAA CTG ATT A-3’ ...
-
bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a QuantStudio 6 Flex thermocycler (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2023Quote: mRNA was isolated from 6-well plates showing a cell confluency of ∼90% using the Monarch Total RNA Miniprep kit (NEB). siRNA transfection was performed 48 hours prior to RNA isolation using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... with a 384 well plate using the NEB Luna Universal One-Step RT-qPCR kit (NEB #E3005L, New England Biolabs Inc) and a reaction volume of 10 μl with 2.5 μl of sample ...
-
bioRxiv - Microbiology 2020Quote: ... with a 384 well plate using the NEB Luna Universal One-Step RT-qPCR kit (NEB #E3005L, New England Biolabs Inc) and a reaction volume of 10 μl with 2.5 μl of sample ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 1 uL BsmbI-v2 Golden Gate Assembly Kit (NEB E1602L), and 15.03 uL nuclease free H2O ...
-
bioRxiv - Molecular Biology 2022Quote: For chitin-based sandwich ELISA 50 μl of chitin magnetic beads (New England Biolabs, Ipswich, MA, USA) were transferred into a 1.5 ml reaction tube ...
-
bioRxiv - Genomics 2020Quote: ... Capture plate wells contained 5 µl of capture solution (1:500 Phusion High-Fidelity Reaction Buffer, New England Biolabs; 1:250 RnaseOUT Ribonuclease Inhibitor ...
-
bioRxiv - Microbiology 2023Quote: ... Desialylation of Calu-3 cells was achieved by incubating cells grown in a 96 well plate or 24-well plate or 6 well plate with 100 U/mL α2-3,6,8,9 neuraminidase A (P0722L, NEB) in 10% FCS media for 3 h at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... 3 µL of RNA from 4 x 96 well plates was transferred to a 384 well plate with 3 µL of master mix containing 1 µL of a 10 mM stock of dNTPs (NEB Catalog# N0447L ...
-
bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
-
bioRxiv - Neuroscience 2023Quote: ... column purified and ligated at a 1:1 vector to insert ratio using the QuickLig Kit (NEB #M2200S). The ASCL1 stop codon was subsequently mutated using the QuickChange II-XL site-directed mutagenesis kit (Agilent #200523 ...
-
bioRxiv - Microbiology 2021Quote: ... 1 µg RNA was reverse transcribed using LunaScript RT SuperMix Kit (NEB). qPCR was performed using synthesised cDNA ...
-
bioRxiv - Cell Biology 2022Quote: ... samples were pooled by plate and ExonucleaseI (NEB) digestion was performed ...
-
bioRxiv - Immunology 2023Quote: All PD-1 mutants were generated using Q5 site-directed mutagenesis kit (NEB) following the manufacture’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... RNA was first circularized by a T4 RNA Ligase 1 Kit (M0204. NEB) and then purified by TRIzol reagent followed by isopropanol precipitation ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was PCR amplified (primers hFUR Exon 1-3 PCR For and hFUR Exon 1-3 PCR Rev) and purified (Monarch PCR & DNA cleanup kit, NEB). The resulting PCR product was sequenced using the hFur Exon 1 Seq primer.
-
bioRxiv - Neuroscience 2021Quote: Libraries were prepared from 1 ug RNA using a library prep kit (NEB #7770), rRNA depletion kit (NEB #E6310) ...
-
bioRxiv - Microbiology 2023Quote: ... 1 μg RNA was reverse transcribed to cDNA using LunaScript RT SuperMix Kit (NEB). qPCR was performed in 20 μl reactions containing diluted cDNA ...
-
bioRxiv - Genetics 2024Quote: ... immunoprecipitated RNA and 1% input RNA Monarch RNA Cleanup Kit (New England Biolabs, T2047L) was used according to the manufacturers’ instructions ...