Labshake search
Citations for New England Biolabs :
1 - 50 of 558 citations for Triaryl Phosphate Isopropylated Grade 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2024Quote: ... Molecular Biology Grade” (NEB) and incubated for 5 min at 3°C ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Molecular Biology Grade (NEB), or Recombinant Albumin ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Molecular Biology Grade (NEB), diluted to 10 mg/mL.
-
bioRxiv - Genetics 2022Quote: NGS libraries were generated by amplifying 12 μl of the eluted CUT&Tag DNA fragments with i5 and i7 barcoded HPLC-grade primers (90) (Suppl. Table 4) with NEBNextHiFi 2x PCR Master Mix (New England BioLabs) on a thermocycler with the following program ...
-
bioRxiv - Biochemistry 2024Quote: ... and the amount of AP activity in the supernatant and lysates was measured at an absorbance of 405 nm over a 90-minute time course following the addition of 1 mg/mL 4-nitrophenyl phosphate (New England Biolabs), using a BioTek SynergyH1 microplate reader ...
-
bioRxiv - Developmental Biology 2024Quote: ... 0.01% Molecular Grade BSA (New England Biolabs), 146 mM NaCl (ThermoFisher Scientific) ...
-
bioRxiv - Plant Biology 2022Quote: ... molecular biology grade (New England Biolabs, B9000S). dNTPs (New England Biolabs ...
-
bioRxiv - Cell Biology 2021Quote: 500mM p-Nitrophenyl Phosphate (pNPP, NEB, P0757S) substrate solution was diluted to 20 mM in phosphatase reaction buffer (PRB ...
-
bioRxiv - Biochemistry 2023Quote: ... In-gel digestion was performed with trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega, Madison, WI; Trypsin-ultraTM, MS grade NEB) with a final trypsin concentration of 20 ng/µl in 50 mM aqueous ammonium bicarbonate and 5 mM CaCl2 ...
-
bioRxiv - Biochemistry 2024Quote: ... When relevant cmpRNA (500 pmol) was 5’-end phosphate-32 “hot” labeled using fresh gamma phosphate-32 labeled ATP (50 nmol) and PNK (NEB) following the producer’s recommendations ...
-
bioRxiv - Molecular Biology 2023Quote: C2566 competent cells (cloning grade) were provided by NEB. ER2948 competent cells were prepared by a modified rubidium chloride method ...
-
bioRxiv - Plant Biology 2024Quote: The hydrolysis rate of p-nitrophenyl phosphate (pNPP, NEB) was determined based on (Wu et al. ...
-
bioRxiv - Biophysics 2022Quote: ... Total of 60 ug of trypsin (mass spectrometry grade, NEB) was diluted in 25 mM NH4HCO3 and added to dried gel pieces (3x volume of the gel volume) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The 5’-phosphate was added via T4 polynucleotide kinase (NEB) at 37 ºC for 30 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were treated with 20µl of ProtK (NEB Molecular Biology Grade), incubated for 3 hrs at 55°C and 5 hrs at 65°C to reverse crosslinks ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 0.45% Tween-20) and Proteinase K (NEB, molecular biology grade) diluted tenfold in NEB storage buffer (20 mM Tris pH 7.4 ...
-
bioRxiv - Microbiology 2020Quote: ... we used a colorimetric assay using p-Nitrophenyl phosphate (pNPP) (NEB) as a substrate ...
-
bioRxiv - Cancer Biology 2024Quote: ... and treated with 800 U of Lambda Phosphates (New England Biolabs) for 30 min at 30°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... 0.5% BSA (molecular grade BSA from Biolabs or Acetylated BSA from Ambion), and Tween-20 at 0.1% ...
-
bioRxiv - Genomics 2024Quote: ... 200 µg/mL molecular biology grade recombinant albumin (rAlbumin) (New England Biolabs B9200S), 0.8 U/µL RiboLock RNase inhibitor (Thermo Fisher Scientific EO0384) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 μg of total RNA was treated with amplification-grade DNase I (NEB) and reverse transcribed using Oligo(dT)20 as a primer and Superscript III Reverse Transcriptase (Life Technologies ...
-
bioRxiv - Plant Biology 2022Quote: ... and 6 µM p-Nitrophenyl Phosphate (pNPP) (New England Biolabs Catalog Number P0757S) at 25°C at a pH of 5.0 ...
-
bioRxiv - Microbiology 2024Quote: A colorimetric assay using the substrate p-Nitrophenyl phosphate (PNPP) (New England Biolabs) was used to quantify alkaline phosphatase activity in colonies grown on MOPS agar plates with no added BCIP using the inoculation regime described above ...
-
bioRxiv - Microbiology 2021Quote: ... 0.5 μL of molecular biology grade bovine serum albumin (20 mg/mL, New England Biolabs), 5 μL of 5× Phire Buffer ...
-
bioRxiv - Microbiology 2021Quote: ... After incubation with 5‘-phosphate-dependent exoribonuclease XRN-1 (New England BioLabs, Inc., USA), samples were treated with RNA 5‘ polyphosphatase (Lucigen) ...
-
bioRxiv - Cell Biology 2023Quote: ... immunoprecipitated beads were incubated with 40 Units of lambda protein phosphate (New England Biolabs) in a 50 μl reaction ...
-
bioRxiv - Biochemistry 2021Quote: ... rinsed with PBS and incubated in PBS containing 3% molecular biology grade BSA (New England Biolabs) and 0.05% Tween-20 for 1 h at room temperature ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Then 0.3 μL of 1 mg mL−1 trypsin (trypsin-ultra, MS-grade, New England Biolabs) was added and samples were incubated at 37 °C overnight ...
-
bioRxiv - Biochemistry 2020Quote: ... The 5’ phosphate of transcribed RNA ligands was removed using calf intestinal phosphatase (CIP, NEB) and then replaced with 32P using T4 polynucleotide kinase (PNK ...
-
bioRxiv - Microbiology 2020Quote: ... 30 mM acetyl phosphate or 1 µl Calf Intestinal Alkaline Phosphatase (CIP) (NEB, catalog#: M0290V) was added to the solution ...
-
bioRxiv - Biochemistry 2023Quote: ... buffer 4 (NEB, identical composition to rCutsmart buffer ...
-
bioRxiv - Systems Biology 2020Quote: ... that had been blocked and equilibrated into the binding buffer supplemented with 0.1% Tween 20 and 0.1μg/μl of BSA (Molecular Biology Grade, NEB). Protein-RNA complexes were then incubated with the magnetic beads on a shaker for further two hours ...
-
bioRxiv - Biochemistry 2022Quote: ... PNGase F (P0704) and mass spectrometry grade Trypsin (Trypsin-Ultra, P8101) are from New England Biolabs (NEB). Fbs1-GYR proteins were expressed and purified from E ...
-
bioRxiv - Microbiology 2020Quote: ... either 30 mM acetyl phosphate or 0.5 μl Calf Intestinal Alkaline Phosphatase (CIP) (NEB, catalog#: M0290V) was added to the solution ...
-
bioRxiv - Biochemistry 2023Quote: ... The tRNA 2’-3’ cyclic phosphate was removed by treatment with T4 PNK (New England Biolabs) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... Dephosphorylation of cyclic phosphate groups were carried out with T4 PNK (10 U/uL, M0201, NEB) in a low pH buffer (5X PNK pH 6.5 buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... Dephosphorylation of cyclic phosphate groups was carried out with T4 PNK (10 U/µL, M0201, NEB) in a low pH buffer (25 mM MES (2-(N-morpholino)ethanesulfonic acid) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 4: 4°C hold) using NEB Next High-Fidelity master mix (NEB) in 25 µl reaction volume using RAD-Marker for/ RAD-Marker rev primers (25 nM each ...
-
bioRxiv - Cell Biology 2020Quote: ... Oct-4 (NEB, D7O5Z), Sox2 (NEB ...
-
bioRxiv - Molecular Biology 2021Quote: ... 4 ml ScaI (BioLabs), followed by 3 h of incubation with a fresh portion ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5% glycerol) and one time with molecular grade water followed by incubation with proteinase K (New England Biolabs) at 55 °C for 30 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5% glycerol) and one time with molecular grade water followed by incubation with proteinase K (New England Biolabs) at 55 °C for 30 minutes with constant shaking ...
-
Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: GBL phosphate (8) was incubated with 5 units of calf intestinal alkaline phosphatase (CIP, New England Biolabs) in 50 mM HEPES buffer (pH 8.0 ...
-
bioRxiv - Plant Biology 2021Quote: ... The antisense strand with a 3′ phosphate was radiolabeled by T4 Polynucleotide Kinase (3’ phosphatase minus) (NEB) and [γ-32P]ATP ...
-
bioRxiv - Microbiology 2020Quote: ... suspended in 200 µL of phosphate saline buffer (PBS) and labeled with TMR-Star (New England Biolabs) for 30 min at 37°C in the dark at a final concentration of 250 nM ...
-
bioRxiv - Physiology 2021Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 [Bio-Rad], 0.01% Molecular Grade BSA [New England Biolabs] ...
-
bioRxiv - Microbiology 2023Quote: 500 infected RBCs were sorted into 3μL of Lysis buffer (final concentration of RNAseOut 2U/μL and 1mg/mL BSA, Molecular Biology Grade (NEB), diluted into sterile PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... Snap-cooled RNAs were diluted to 8 nM in 2x RNA master mix (0.2 mg/mL bovine serum albumin (molecular biology grade, NEB), 0.2 mg/mL yeast tRNA ...
-
bioRxiv - Biochemistry 2024Quote: ... then diluted to 8 nM concentration in 2x RNA master mix (0.2 mg/mL bovine serum albumin (molecular biology grade, NEB), 0.2 mg/mL yeast tRNA ...
-
bioRxiv - Molecular Biology 2021Quote: ... γ-phosphate was then removed from excess [γ-32P]ATP with 0.5 U apyrase (New England Biolabs M0398) in 1X apyrase buffer for 10 min at 30°C ...