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Citations for New England Biolabs :
1 - 50 of 1139 citations for Tetratricopeptide repeat protein 14 TTC14 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... PCR across the 70-bp repeats were carried out with LongAmp enzyme (NEB) according to standard protocols with primers PseudoF and PACR or RFPF and VSG221R.
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bioRxiv - Genetics 2020Quote: ... Repeats were amplified with high fidelity using the LongAmp Taq polymerase (New England Biolabs). Twenty to 50pg of DNA was amplified in a 25 µL reaction mix containing 2.5 units LongAmp Taq DNA polymerase ...
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bioRxiv - Biophysics 2021Quote: ... according to the protocol for cloning DNA containing repeat elements (C3040, New England BioLabs Inc.). In both plasmids ...
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bioRxiv - Genomics 2024Quote: ... 14 μL of dNTP (NEB N0446S) (dATP-14-biotin and dTTP ...
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... Expansions too large for direct sequence (approximately >250 repeats) were digested with MspA1I (New England Biolabs) which ambiguously digests the PCR products containing either CGG or CTG interruptions in the CAG direction of the repeat tract ...
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bioRxiv - Cell Biology 2023Quote: ... 14-cycle PCR with index primers (NEB) was performed (initial denaturation ...
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bioRxiv - Genomics 2024Quote: Library prep kit(s) (e.g., EpiCypher 14-1001 or 14-1002 [each provides different 48x multiplex barcode sets] or NEB NEBNext Ultra II DNA Library Kit E7645 ...
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bioRxiv - Plant Biology 2021Quote: ... The talIMAI1 central repeat region was then swapped into pSKX1_talFMAI1ΔAD using StuI and AatII (NEB, New England Biolabs) to generate pSKX1_talIMAI1ΔAD ...
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bioRxiv - Plant Biology 2021Quote: ... The talIMAI1 central repeat region was then swapped into pSKX1_talFMAI1ΔAD using StuI and AatII (NEB, New England Biolabs) to generate pSKX1_talIMAI1ΔAD ...
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bioRxiv - Cancer Biology 2023Quote: ... Oligonucleotide probe for telomere or Alu repeats was labeled with [32P]-ATP (3,000 Ci/mmol) and T4 nucleotide kinase (New England Biolabs). The membrane was hybridized in Church hybridization buffer containing a 32P-labeled probe at 42°C overnight ...
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bioRxiv - Biophysics 2024Quote: ... into a pSGEM construct with five consecutive repeats of the myc tag (Lörinczi et al. 2015)) using NcoI and HindIII (New England Biolabs).
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bioRxiv - Genetics 2024Quote: ... but the CAG repeat was targeted with crRNA sequence (CTGCTGCTGCTGCTGCTG, Integrated DNA Technologies) and PspXI restriction enzyme (New England Biolabs). The 0.8 kb fragment with 115 uninterrupted repeats and 12 kb digested fragment with the CAACAG-dup and CAA/CCA-loss variants contain homology sequences within the CAG repeat itself (3’ and 5’ ends of fragments ...
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bioRxiv - Cell Biology 2024Quote: ... we replaced the EGFP from the previously generated EGFP-Myo10MD-FRB (above) with GCN4 repeats from pHRdSV40-K560-24xGCN4_v4 (addgene #72229) via Gibson assembly (NEB #E2621S) using the following primer sets ...
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bioRxiv - Microbiology 2021Quote: ... and fifteen repeats of the FG-repeats were amplified from genomic DNA using primers (Table S2) with Phusion High-Fidelity PCR Kit (NEB - E0553S). The PCR products were purified with GenElute PCR Clean-Up Kit (Sigma-Aldrich NA1020) ...
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bioRxiv - Molecular Biology 2021Quote: ... The SCR and its truncated version (tSCR or Ei) were cloned between the piggyBac transposon-specific inverted terminal repeat sequences (ITR) by linearizing the vector with BamHI (NEB, R3136) and NheI (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was then hybridized at 65°C overnight with a double-stranded 800 bp telomeric repeat probe (Telo800) that was radioactively labeled with Klenow (NEB M0210S) and [α-32P]dCTP (PerkinElmer BLU513H) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Maltose Binding Protein monoclonal antibody IgG2a (New England Biolabs), mouse anti-Histone H3 [Trimethyl Lys9] 6F12-H4 (Novus Biologicals) ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were then coupled to protein G beads (New England Biolabs). After beads were washed ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were detected using anti-MBP antibody (anti-MBP NEB e8032s) and HRP conjugated secondary antibody ...
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bioRxiv - Cell Biology 2022Quote: ... mTagBFP2 and Cas9-Avidin provided by Max Wilson into an expression vector bearing a CMV promoter and flanking PiggyBac transposase-compatible inverted terminal repeats using Gibson Assembly (New England BioLabs Inc., E2611L) according to supplier instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Interacting MBP-AtPIP5K2 protein was detected using an anti-MBP antibody (NEB). Protein input was detected using an anti-GST antibody (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... cDNA was PCR amplified for 14 cycles by using LongAmp Taq (NEB). ONT adaptor ligation was performed using T4 DNA ligase (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... with 14 PCR cycles using unique dual indexes (New England Biolabs E6440S). Following clean-up with SparQ PureMag beads (QuantaBio) ...
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bioRxiv - Systems Biology 2024Quote: ... and 14 μL Bst2.0 8,000 U/mL [100 U/mL final] (NEB) was added to the sample ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid expressing EBFP2-14-3-3 theta was created using HiFi Cloning (NEB) of 14-3-3 theta into pEBFP2-C1 (Addgene plasmid #54665) ...
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bioRxiv - Microbiology 2024Quote: ... pCas9/sg plasmid (courtesy of Robert W. Moon, (14)) was digested with BtgZ1 (NEB) for 2 hours and purified using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Biochemistry 2021Quote: ... 1μL m6A antibody per sample was coupled to pre-washed Protein G Magnetic Beads (NEB) in 1x reaction buffer (150mM NaCl ...
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bioRxiv - Genetics 2021Quote: ... 0.5 μM MPRA_v3_F and MPRA_v3_20I_R primers (Supplementary Table 14) and 2 ng BSA (NEB, B9000). PCR master mix was emulsified by vortexing with 220 μL Tegosoft DEC (Evonik) ...
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bioRxiv - Cancer Biology 2020Quote: ... extra Biotin-14-dATP was first removed using 15 units of T4 DNA polymerase (NEB) and incubating at 20°C for 4 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... The antibodies were captured using 50 µl of protein G magnetic beads (S1430, New England Biolabs) incubated for 60 min with end over end mixing at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate-antibody mix was then incubated with Magnetic Protein A beads (New England Biolabs, S1425S) overnight at 4 °C with end over rotation ...
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bioRxiv - Microbiology 2020Quote: ... 14-17 A260 of lysate was digested with 800 U/A260 of micrococcal nuclease (MNase, NEB) at 25°C with shaking at 14,500 rpm for 20 min in lysis buffer supplemented with 2 mM CaCl2 and 500 U RNase Inhibitor ...
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bioRxiv - Plant Biology 2020Quote: ... Resulting fragments were amplified for 14 cycles using NEBNext Ultra II PCR protocol (New England BioLabs) and quality was assessed with the Agilent High sensitivity DNA kit ...
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bioRxiv - Genomics 2024Quote: ... and amplified with 14 cycles of PCR using LongAmp Taq Master Mix (New England Biolabs, UK). cDNA was quantified using the Qubit DNA High sensitivity assay (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
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bioRxiv - Cell Biology 2021Quote: ... 14 μg of sample DNA were digested overnight at 25°C with CviQI restriction enzyme (NEB, R0639L), which cuts outside the region of interest.
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 ul 10 mM dGTP, 1.5 ul 10 mM dTTP, 37.5 ul 0.4 mM biotin-14-dATP, 40 ul NEB buffer2 ...
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bioRxiv - Developmental Biology 2020Quote: ... 37.5 μl of 0.4 mM biotin-14-dATP and 10 μl of 5 U/ μl Klenow (NEB, M0210L) were added and mixed by pipetting ...
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bioRxiv - Microbiology 2020Quote: ... glabrata total cell lysates using the anti-Rad53 antibodies described in the previous section and Protein A magnetic beads (New England Biolabs). The immunoprecipitated samples were run on 8% acrylamide gels ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5% Nonidet P-40) and incubated with pre-washed anti-D-lactyllysine antibody-conjugated protein A agarose beads (PTM Biolabs) at 4 °C overnight ...
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bioRxiv - Genetics 2021Quote: ... Tagmented library was amplified with P5_BRB and BRB_Idx7N5 primers (5 μL, Supplementary Table 14) using NEBNext UltraTM II Q5 Master Mix (NEB, M0544L) which was incubated at 98 °C for 30 sec before adding DNA with the following conditions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... with 10–14 cycles of enrichment for each sample and using Phusion High-Fidelity Master Mix (NEB catalog #M0531). Samples were submitted for 101 bp paired-end sequencing on an Illumina HiSeq2000 at BGI-Hong Kong ...
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bioRxiv - Microbiology 2021Quote: ... First-round PCR of 14 cycles was performed using Q5 High-Fidelity Master Mix (New England BioLabs, Ipswich, MA) and nested gene-specific primers at 20 μM ...
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bioRxiv - Genomics 2021Quote: DNA ends were marked with biotin-14-dATP by adding 60μL of biotin fill-in master mix (1X NEB 3.1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 7.5 µL DNA was incubated (14 h, 37°C) with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix ...
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bioRxiv - Cancer Biology 2023Quote: ... The reactions were performed in triplicate for 14 cycles using Q5 Hot Start High-Fidelity 2X Master Mix (NEB), pooled and purified using AMPure XP beads (Beckman Coulter).
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bioRxiv - Neuroscience 2024Quote: ... DNA contained in the eluate was then amplified for 14 cycles in 25μl PCR reactions using NEBNext High-Fidelity Q5 2X PCR Master Mix (NEB) and 0.5 mM each of Solexa 1GA and Solexa 1GB primers ...
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bioRxiv - Biochemistry 2022Quote: ... protein was treated overnight with Lambda Protein Phosphatase (NEB #P0753S) at 4 °C.
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bioRxiv - Neuroscience 2021Quote: ... Cas9 protein (NEB) was mixed with all six sgRNAs and injected into embryos at the 1-cell stage ...
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bioRxiv - Plant Biology 2024Quote: ... SpCas9 protein (NEB), 1 x cut smart buffer (NEB) ...