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Citations for New England Biolabs :
101 - 150 of 2194 citations for TRNA cytosine 5 Methyltransferase TRDMT1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then washed with 1xPBS and blocked for 30 minutes at 37°C in blocking buffer (1xPBS, 0.05% BSA [Invitrogen, Am2616], 50μg/mL yeast tRNA [Sigma-Aldrich, R8759], 10mM Vanadyl Ribonucleoside complex [New England Biolabs, S1402S] ...
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bioRxiv - Cell Biology 2022Quote: ... The mutated Rop1 expression plasmid and the amber suppressor tRNA plasmid were co-transformed into BL21 (DE3) cells (NEB), as described2 ...
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bioRxiv - Genomics 2021Quote: SAMOSA was performed on remodeled arrays as well as unremodeled arrays and unassembled DNA controls using the non-specific adenine EcoGII methyltransferase (New England Biolabs, high concentration stock 2.5e4 U/mL) as previously described (Abdulhay et al. ...
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bioRxiv - Genomics 2020Quote: ... Biotin labeled dATP (Thermo,19524016) and Klenow (NEB, M0210) were used to fill restriction fragment overhangs ...
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bioRxiv - Biophysics 2019Quote: ... fusion proteins were directly biotinylated with BG-Biotin (NEB), buffer exchanged with a Zeba Desalting Column (ThermoFisher ...
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bioRxiv - Developmental Biology 2021Quote: ... They were then incubated overnight at 37 °C in Hybridization Buffer (10% Formamide, 10% 20x SSC, 400 µg/ml E. coli tRNA (New England Biolabs), 5% dextran sulfate ...
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bioRxiv - Synthetic Biology 2019Quote: ... An in vitro RED20 prototype was prepared by supplementing PURExpress in vitro expression system lacking tRNAs (PURE ΔtRNA, NEB# E6840S). PURE ΔtRNA supplemented with supplied control tRNAs was used as a Standard Code ...
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bioRxiv - Bioengineering 2021Quote: ... and 10 pmol of unligated tRNA sample were prepared in an equivalent buffer to the first ligation reaction (1X RNA Ligase 2 buffer (NEB), 5% PEG 8000 ...
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bioRxiv - Plant Biology 2020Quote: ... the chain of multiplex tRNA-gRNA with three NsWOX9-spacers was inserted into an optimized vector with AtU6-tRNA-gRNAs-AtUbi10-Cas9-pRGEB31-bar backbone by digestion and ligation using Fok I (NEB) and BsaI enzymes (Xie ...
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bioRxiv - Molecular Biology 2019Quote: ... tRNA containing plasmids were linearized using restriction digestion and tRNAs were in vitro transcribed overnight using the HiScribe T7 transcription kit (NEB) per the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... 10% Dextran sulfate Sigma D8906; 0.02% BSA Ambion AM2616; 1 mg/ml E.coli tRNA Sigma R1753; 2 mM Vanadyl-ribonucleoside complex NEB S1402S; 2XSSC) containing diluted probes and incubated over night at 30°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fragments of 10 - 40 nt for partially hydrolyzed and re-phosphorylated tRNAs were converted to small RNA sequencing libraries using NEBNext multiplex small RNA library prep set (NEB) for Illumina kit ...
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bioRxiv - Biochemistry 2020Quote: ... Mature tRNA sequences were obtained by deleting the intron sequence using the Q5 Site-Directed Mutagenesis kit (New England Biolabs). All constructs in this study were verified by Sanger sequencing.
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bioRxiv - Synthetic Biology 2022Quote: ... The genomic tRNA operon of REP12 was PCR amplified using the Q5 Hot Start Master Mix (New England Biolabs, USA) from extracted phage gDNA and purified using the GeneJet PCR purification kit (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Radioactively labelled tRNAs carrying a 2′,3′ cyclic phosphate at the 3′ end was dephosphorylated using T4 polynucleotide kinase (NEB) in 100 mM Tris-HCl pH 6.5 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 100 ng of the amplified tRNA operon was assembled into the linearized pRK2-cat backbone using the HiFi DNA Assembly Master Mix (New England Biolabs, USA). After incubation for 60 mins at 50 °C ...
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bioRxiv - Genomics 2023Quote: ... Included in the gel as controls were an RT reaction conducted in the absence of tRNA and a Small Range RNA Ladder (NEB N0364S). The gel was stained with SYBR gold and visualized using a ChemiDoc imager (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... and tumbled with 5 μg of anti-m6A antibody (New England Biolabs) at 4°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... and then hybridized overnight at 37°C in hybridization buffer (2xSSC, 10% dextran sulfate [Sigma-Aldrich, D8906], 1mg/mL yeast tRNA [Sigma-Aldrich, R8759], 2mM Vanadyl Ribonucleoside complex [New England Biolabs, S1402S] ...
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bioRxiv - Molecular Biology 2021Quote: ... were then combined with 10 μM CLIP-Biotin (New England Biolabs) and rotated (end-over-end ...
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bioRxiv - Genomics 2019Quote: ... Biotin labelled DNA was pulled down on Streptavidin Dynabeads (NEB, S1420S). After DNA repair and 3’ A addition ...
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bioRxiv - Genomics 2019Quote: ... Biotin labelled DNA was pulled-down on Streptavidin Dynabeads (NEB, S1420S). After DNA repair and 3’ A addition ...
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bioRxiv - Genomics 2023Quote: ... dTTP and biotin-dATP using Klenow DNA polymerase I (NEB M0212L), followed by in situ proximity ligation at room temperature overnight ...
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bioRxiv - Synthetic Biology 2022Quote: In vitro transcription/translation by codon skipping of the short FLAG tag-containing peptides X-Val-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (XV-Flag) where X = 7, 13, 14, or 15 was carried out using the PURExpress® Δ (aa, tRNA) Kit (New England Biolabs, E6840S) based on a previous protocol with slight modifications 16 ...
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bioRxiv - Biophysics 2019Quote: ... the imaging chamber was incubated in blocking buffer (10 mM Tris HCl pH 7.5, 50 mM NaCl, 1 mg/mL BSA [NEB], 1 mg/mL tRNA [Ambion]) for 1 h ...
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bioRxiv - Biophysics 2022Quote: ... rk430-mScarlet-SNAP (7 μM monomer) was incubated with benzylguanine-biotin (NEB) in a 4 to 1 molar ratio at room temperature for 15 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... SNAP reagents (BG-649, BG-fluorescein and BG-biotin) were obtained from NEB. Electrocompetent cells were used in all transformations.
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bioRxiv - Cell Biology 2021Quote: ... and biotin-pupE labelled proteins were enriched by streptavidin magnetic beads (NEB, S1420S) and identified by mass spectrometry analysis (Liu et al. ...
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bioRxiv - Biophysics 2023Quote: ... The biotin-modified DNA was digested using a KasI restriction enzyme (NEB #R0544S) that produced two fragments of 24 kb and 24.5 kb ...
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bioRxiv - Molecular Biology 2020Quote: ... Digested fragments were biotin-labeled and subsequently ligated by T4 DNA ligase buffer (NEB) for 2 hours at 23°C with 300 rpm gentle rotation ...
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bioRxiv - Microbiology 2022Quote: ... The remaining volume was submitted for biotin-enrichment on magnetic Streptavidin beads (NEB S1420S), by vortexing for 90 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... and biotin-labelled proteins were isolated by immunoprecipitation using streptavidin magnetic beads (NEB, S1420S) in an immunoprecipitation buffer containing 25 mM Tris-HCl ...
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bioRxiv - Genetics 2023Quote: ... biotin from un-ligated DNA ends was removed using T4 DNA polymerase (NEB #M0203) and the DNA was sonicated (Covaris E220 ...
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bioRxiv - Cancer Biology 2020Quote: ... extra Biotin-14-dATP was first removed using 15 units of T4 DNA polymerase (NEB) and incubating at 20°C for 4 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.167 mg/mL MTSEA-biotin) for 30 min and purified with Streptavidin Magnetic Beads (NEB). The biotinylated RNA was eluted with 100 mM DTT and purified with RNA Clean beads (Vazymes) ...
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bioRxiv - Biochemistry 2020Quote: ... m7G(5′)ppp(5′)G (NEB, #S1404S) and m7G(5′)ppp(5′)A (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
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bioRxiv - Genomics 2020Quote: ... biotin-dNTPs at the DNA termini were removed with 500 units of Exonuclease III (NEB #M0206) in 1X NEBuffer 1 (NEB #B7001 ...
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bioRxiv - Biophysics 2020Quote: ... A 478 bp fragment of biotin labelled DNA was produced by PCR with Taq Polymerase (NEB) of λ-DNA using the following primers ...
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bioRxiv - Microbiology 2020Quote: ... Biotin-labeled RNP lysates were supplemented with streptavidin beads (1 mL per replicate, New England Biolabs) and captured by rotating at room temperature overnight ...
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bioRxiv - Biochemistry 2020Quote: ... and m7G(5′)ppp(5′)A (NEB, #S1405S) also referred throughout the manuscript as N7-meGpppG and N7-meGpppA for consistency ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µL RNase H (5 U/µL, NEB), 2.5 µL RNase III (1 U/µL ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’-hydroxyl (5’HO-RNA30-FAM-3’) or 5’-Gppp (5’Gppp-RNA30-FAM-3’) in 1x NEBuffer 3 (NEB; B7003), in 20% denaturing polyacrylamide gels ...
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bioRxiv - Plant Biology 2020Quote: ... 5 µl Taq 5× Master Mix (New England Biolabs) and 18 µl Milli-Q water (18.2 MΩ cm) ...
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bioRxiv - Developmental Biology 2023Quote: ... then capped with m7G(5’)ppp(5’)G (NEB) and tailed with a poly(A ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotin-dNTPs at the unligated DNA termini were removed by ∼500 units of Exonuclease III (NEB #M0206) in 1X NEBuffer 1 (NEB #B7001 ...
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bioRxiv - Cell Biology 2019Quote: ... 1 µl of 1 mM BG-(PEG)12-biotin (New England Biolabs; PEG linker available on request) was added to 200 µl of axonemes ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µl of 1 mM BG-(PEG)12-biotin (New England Biolabs; PEG linker available on request) was added to 200 µl of axonemes ...
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bioRxiv - Molecular Biology 2023Quote: ... SNAP-tag coupling activity (methodology described below) was first tested with a small ligand BG-biotin (NEB) or DNA (a 550 bp benzylguanylated PCR product ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 ul 10 mM dGTP, 1.5 ul 10 mM dTTP, 37.5 ul 0.4 mM biotin-14-dATP, 40 ul NEB buffer2 ...