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Citations for New England Biolabs :
1 - 50 of 101 citations for TMB Substrates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated antibody binding was detected using TMB substrate (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... plates were washed 3 times with PBS-T and TMB substrate (Denovo Biolabs) was added for development of color ...
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bioRxiv - Cell Biology 2020Quote: ... CLIP-Cell TMR star substrate or CLIP-Surface 488 substrate (NEB S9219 and S9232) was reacted with dCas9-CLIP proteins at 37°C for 60min ...
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bioRxiv - Microbiology 2020Quote: ... The TMR-Star substrate (New England Biolabs) was added at 250 nM ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescent cell permeable Snap-Cell substrates (NEB) were used in the following concentrations ...
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bioRxiv - Biophysics 2020Quote: Gaussia and Cypridina substrates were obtained from NEB or its original supplier ...
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bioRxiv - Microbiology 2021Quote: ... or 100 nM TMR substrate for SNAPtag (NEB) for 30 minutes at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... in vitro RNA methylation experiments were performed for 2 h at 37 °C in 20 μL reaction mixtures containing 1× CutSmart buffer with 5 μg RNA substrate (1.8 knt. Same substrate of MTase-Glo experiment) and 1 unit RNase Inhibitor (New England Biolabs Inc., Massachusetts, USA), 1 μM methyltransferase or no enzyme as a negative control ...
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bioRxiv - Molecular Biology 2019Quote: ... The substrates were amplified using Phusion Polymerase PCR (NEB). The linear double-stranded molecules were denatured and renatured to produce both the parental linear molecules and a circle with nicks on each strand nearly half-way around the circle which were then ligated by Taq Ligase (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.3 µM fluorescent SNAP substrate (TMR Star, NEB, S9105) was added along with the primary antibodies
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bioRxiv - Microbiology 2021Quote: ... or 100 nM TMR substrate for SNAPtag (S9105S, NEB), for 30 min anaerobically ...
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bioRxiv - Cell Biology 2023Quote: ... SNAP-Cell 647-SiR substrate (S9102S New England Biolabs) was used to reveal SNAP-tag following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Signal was detected with Clarity™ ECL Blotting Substrate (NEB) and on a GE Imagequant LAS 4000 mini ...
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bioRxiv - Biophysics 2020Quote: ... for the substrate-binding domain mutants and βamHI-AlwnI (NEB) for the transmembrane domain mutants (Appendix) ...
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bioRxiv - Molecular Biology 2023Quote: ... or ΦX174 RF I DNA (dsDNA substrate, NEB, Ipswich MA) into buffer containing 50 mM Bis-Tris pH 6.0 ...
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bioRxiv - Biophysics 2021Quote: ... After 24 hours SNAP-Cell 647-SiR substrate (New England Biolabs) was added to the medium at a concentration of 3 μM ...
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bioRxiv - Biochemistry 2020Quote: Translocation substrates were synthesized in vitro using the PURExpress system (NEB) in the presence of [35S]-methionine for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... the substrates used are 5’-end-labeled with T4 PNK (NEB) in the presence of gamma 32P-ATP ...
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bioRxiv - Microbiology 2019Quote: ... The double-stranded DNA substrate pUC19 (New England Biolabs, MA, USA) was linearized by digesting with PstI (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... Circular ssDNA (M13MP18) substrate (0.5 µg, New England Biolabs, MA, USA) was combined with 4 pmol of each cellular nuclease alone and in combination ...
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bioRxiv - Cell Biology 2019Quote: ... cells were labeled with Oregon-Green SNAP substrate (New England Biolabs) at 4 µM final concentration for labeling of the newly synthesized CENP-A molecules ...
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bioRxiv - Biochemistry 2021Quote: ... the pre-crRNA substrates were 5′-radiolabeled with T4 PNK (NEB) in the presence of gamma 32P-ATP ...
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bioRxiv - Biochemistry 2023Quote: ... The dsDNA substrate was a ~10-kb Nt.BbvCI-nicked (NEB #R0632) pMYC plasmid used at 2.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the dsDNA substrate was a ~10-kb Nt.BbvCI-nicked (NEB #R0632) pMYC plasmid used at 5 ng/µL (~0.83 nM) ...
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bioRxiv - Molecular Biology 2022Quote: 5’-end 32P-labelled DNA substrates were generated using T4 PNK (NEB) and [ɣ-32P]ATP (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: ... DNA substrates were 5’ terminally labelled with T4 Polynucleotide Kinase (NEB, M0201S) and ATP-γ-32P (PerkinElmer ...
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bioRxiv - Biochemistry 2020Quote: ... The substrate was then treated with 50 U of uracil DNA glycosylase (NEB) for 2 h at 37°C to form the abasic site ...
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bioRxiv - Biophysics 2020Quote: ... the cells were first labelled with 5 µM biotin-conjugated SNAP-tag substrate (NEB) in tissue culture medium ...
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bioRxiv - Molecular Biology 2022Quote: DNA substrates for gel shift assays were amplified by PCR using Q5 Polymerase (NEB) and purified using a DNA Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg of the DNA substrate were digested with 30U of Nb.BbvCI (from NEB) for 2 h at 37 °C ...
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bioRxiv - Genetics 2020Quote: DNA substrates were generated using Q5 High-Fidelity DNA Polymerase (M0491S; New England BioLabs) to PCR-amplify pDEST-BLRR with TIDE-1-fwd and TIDE-1-rev (Supplementary Table 1 ...
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bioRxiv - Plant Biology 2021Quote: ... followed by detection of ubiquitinated substrate by immunoblotting using anti-MBP (New England Biolabs), anti-GST and anti-ubiquitin (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... The chemical substrates were SNAP- tag ligands (SNAP surface 549 - BG 549 [NEB, S9112S]) and CLIP-tag ligands (CLIP surface 647 - BC 647 [NEB ...
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bioRxiv - Biochemistry 2021Quote: ... RNA substrates were 5’ end labeled using γ32P-ATP and T4 Polynucleotide Kinase (NEB, MA)38 ...
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bioRxiv - Biochemistry 2021Quote: ... or Endoglycosidase H (translation products of ∼50-150 kDa protein substrates; New England Biolabs, P0702S). All samples were solubilised for 12 h at 37°C and then sonicated prior to resolution by SDS-PAGE (10% or 16% PAGE ...
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bioRxiv - Biochemistry 2021Quote: RNA substrates (listed in Table S1) were either 5’ labelled by T4 polynucleotide kinase (NEB) and 5’ 32P-γ −ATP ...
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bioRxiv - Neuroscience 2020Quote: ... PBT was removed and SNAP substrate diluted in PBT (SNAP-Surface649, NEB S9159S; 1:1000) added ...
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bioRxiv - Biochemistry 2021Quote: ... The deaminations were detected by treating the substrates with Uracil DNA Glycosylase (New England Biolabs) and heating under alkaline conditions ...
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bioRxiv - Microbiology 2021Quote: ... 5μM diphosphorylated 20mer RNA substrate was pre-treated with 0.5U/μl RNA 5’ Pyrophosphohydrolase (NEB) in 1× NEBuffer 2 (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... and nucleosome substrates were immobilized on an equal volume of streptavidin-coated magnetic beads (NEB), pre-washed and resuspended in 2x quench buffer ...
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bioRxiv - Plant Biology 2022Quote: ... 5 pmol of label-free RNA substrates were dephosphorylated with Alkaline Phosphatase (New England Biolabs) and labeled with [γ-32P] ATP (PerkinElmer ...
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bioRxiv - Synthetic Biology 2023Quote: ... To prepare the TEV substrate two oligos were annealed using T4 Polynucleotide Kinase (NEB#M0201S) and T4 DNA ligase buffer (NEB#B0202S) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA substrates were synthesized by run off in vitro transcription (IVT) using T7 polymerase (NEB) in the presence of [α-32P]-UTP according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 1pmol ssDNA substrate 5’ DY782 ATTATTATTATTATTATTATTTCATTTATTTATTTATTTA-3’ (Eurofins, UK) and 0.75U uracil-DNA glycosylase (NEB) were added to 10µg protein lysate at 37°C for 1 hour ...
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bioRxiv - Plant Biology 2023Quote: ... followed by detection of the ubiquitinated substrate by immunoblotting using anti-MBP (New England Biolabs), anti-GST and anti-ubiquitin (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA substrate was incubated with 1 unit of purified Uracil-DNA glycosylase (New England BioLabs) instead of cell extracts in the same reaction buffer ...
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bioRxiv - Cell Biology 2020Quote: ... peak fractions pooled and reacted with 2-molar excess SNAP-substrate Alexa-488 dye (S9129, NEB) overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: m7G-capped RNA substrates 90 to 1400 nucleotides long were generated by T7 RNA polymerase (NEB) transcription of different DNA templates generated from an FLuc plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... The substrate was obtained by PCR on CML cells derived gDNA using Q5 DNA polymerase (NEB) and purified on a 10g/L agarose gel using the Wizard SV Gel and PCR Clean-Up system (Promega) ...
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bioRxiv - Biophysics 2021Quote: ... The cells were then incubated with the SNAP substrate (Alexa 647 – benzylguanine, 1 µM, NEB#S9136S) in 0.5% BSA + 1mM DTT at room temperature for 2 hours ...