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Citations for New England Biolabs :
101 - 150 of 8829 citations for Superoxide Anion Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The PCR assays using NEB enzyme Q5® Hot Start High-Fidelity (NEB, M0491) with the following thermocycling conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein amounts for deadenylation assays were estimated against BSA standards (New England Biolabs, B9000S).
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bioRxiv - Microbiology 2023Quote: ... LucEV-A71 assay: The plasmid was linearised using Mlu1 restriction enzyme (NEB, Cat # R3198S), and purified using Phenol:Chloroform:Isoamyl Alcohol (PCI 24:25:1 ...
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bioRxiv - Cancer Biology 2023Quote: ... and regularly tested for mycoplasma contamination (PCR-based assay from Minerva-Biolabs, Berlin, Germany).
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bioRxiv - Genomics 2022Quote: Ribosomal RNA depletion kit (rRNA Depletion Kit, E6310L, NEB)
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bioRxiv - Genetics 2021Quote: ... by PCR amplification of target locus followed by T7 Endonuclease I assay (New England Biolabs).
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bioRxiv - Bioengineering 2020Quote: The following components comprised the RT-LAMP assay: 4 mM of MgSO4 (New England Biolabs), 1× final concentration of the isothermal amplification buffer (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... The assay mixture was then analyzed using western blotting by anti-MBP (New England Biolabs) and anti-His (D2951 ...
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bioRxiv - Immunology 2021Quote: ... Enzymatic assays for PNGase F and Endo S were performed according to manufacturer’s instructions (NEB). The effect of the enzymatic assays was either analyzed by Coomassie staining or immunoblotting.
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bioRxiv - Biochemistry 2023Quote: The exonuclease T degradation assays were performed as described by the manufacturer (New England BioLabs). Reactions (20 μl ...
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bioRxiv - Developmental Biology 2024Quote: ... cutting efficiency was determined by T7-endonuclease assay (T7 Endonuclease I, New England Biolabs, M0302) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Monarch kit (NEB) extracted and 0.1 pmol of each DNA fragment was CPER amplified in a 50 µL reaction containing 200 µM of dNTPs ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrations measured using Qubit 1X dsDNA HS Assay) was treated with 5U of RNAse HI (NEB) in RNAse HI buffer for 30 min at 37°C and then nucleic acids were purified with GeneJET Gel Extraction and DNA cleanup micro kit (General cleanup protocol ...
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...
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bioRxiv - Microbiology 2021Quote: ... The assay mixture was then analyzed using western blotting by anti-PanKcr (PTM-501, PTM Biolabs) and anti-H3 (ab1791 ...
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bioRxiv - Bioengineering 2019Quote: ... This was subjected to a T7E1 assay following the manufacturer’s instructions (New England Biolabs, Ipswich, MA). In brief ...
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bioRxiv - Microbiology 2022Quote: PCR was performed using colonies from transfer assays using OneTaq (New England BioLabs Inc., Ipswich, MA). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... The guide RNA target regions were amplified from transfected cells and analyzed by T7endo1 assay (NEB) to detect genome editing activity at the target site ...
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bioRxiv - Genomics 2022Quote: ... w/o any promoter for promoter-assays were first digested using EcoRV (New England Biolabs, R3195S). Next ...
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bioRxiv - Biochemistry 2023Quote: DNA templates for the transformation assays were prepared by PCR amplification from the pUC19 plasmid (NEB), after which the PCR fragments were Dpn1-treated ...
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bioRxiv - Developmental Biology 2024Quote: ... All constructs generated in this study were obtained using the Gibson assembly assay (NEB, Cat # E2611S).
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bioRxiv - Neuroscience 2021Quote: ... T7 HiScribe kit (NEB) was used to transcribe the sgRNA DNA template with the appended T7 promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... The binding assay was performed by incubating these radiolabelled DNA fragments with RNA polymerase holoenzyme (NEB #MO551S) for 5min at 25 °C followed by loading in 4% polyacrylamide gel in 1X TBE buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The beads to which GFP-DSB-1 had been immobilized were subject to the phosphatase assay (NEB lambda phosphatase #P0753S ...
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bioRxiv - Bioengineering 2019Quote: ... The samples had been measured the concentration by Bradford assay and then treated with enterokinase (NEB Biolabs) overnight ...
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bioRxiv - Bioengineering 2019Quote: ... The samples had been measured the concentration by Bradford assay and then treated with enterokinase (NEB Biolabs) overnight ...
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bioRxiv - Microbiology 2022Quote: ... The SYBR Green assay was performed with the Luna Universal qPCR Master Mix (NEB, Ipswich, MA, USA) according to the manufacturer’s instructions with primers specific for HSV-1 gB (UL27 gene ...
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bioRxiv - Cell Biology 2022Quote: ... For CAK binding assays CDK2 was dephosphorylated by washing beads once in 1x λ-phosphatase buffer (NEB) supplied with 1 mM MnCl2 (NEB ...
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bioRxiv - Microbiology 2022Quote: ... For the noncapped assay the RNA in the reaction was treated with T4 PNK (NEB, Ipswich, MA) to produce a pool of 5’ monophosphate RNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time PCR was performed using SYBR green gene expression assays (New England Biolabs, catalog no. M3003S) on a QuantStudio 6 instrument (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... and a subset of F0 embryos were dissociated and used in T7 endonuclease activity assays (NEB E3321) in order to validate successful CRISPR-Cas9 activity ...
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bioRxiv - Biochemistry 2023Quote: ... in a black-bottomed 96-well plate and gene-specific LA qPCR assays were performed as described earlier18,19,41,42 using Long Amp Taq DNA Polymerase (New England BioLabs). Three transcribed (HPRT ...
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bioRxiv - Cancer Biology 2024Quote: The dePARylation activity of PARG was measured in the PARP1 Histone H4 Activity Assay (#K611, Tulip BioLabs). Briefly ...
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bioRxiv - Genomics 2021Quote: ... using the Golden Gate assembly kit (NEB® Golden Gate Assembly Kit #E1601). Each guide sequence was cloned with its own U6 promoter and was followed by a sgRNA scaffold ...
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bioRxiv - Microbiology 2023Quote: ... A RNA cleanup kit (Monarch RNA Cleanup Kit T2050, New England Biolabs, France) was used to further clean the RNA samples ...
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bioRxiv - Synthetic Biology 2022Quote: ... plasmid (Monarch® PCR mini-prep kit) and gel extraction kits (Monarch® DNA gel extraction kit) were also obtained from NEB and used according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... on ice for 30 min and protein concentration measured by BCA assay (7780, New England Biolabs, Beverly, USA). Results were analyzed using the Compass software (ProteinSimple) ...
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bioRxiv - Microbiology 2021Quote: ... 20 µl of supernatant was mixed with 50 µl BioLux Gaussia Luciferase Assay substrate (New England Biolabs, Germany) and the luciferase activity was determined in a ClarioStar Plus plate reader (BMG Labtech ...
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bioRxiv - Molecular Biology 2022Quote: LAMP assays were optimized with Loxosceles similis DNA using Master Mix reagent (WarmStart® #M1800 – New England BioLabs). For this ...
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bioRxiv - Molecular Biology 2022Quote: ... end-point PCR assays were carried out with Hot Start Taq 2X Master Mix (New England Biolabs, USA) in a 20 μL reaction mixture containing 0.5 units of taq polymerase ...
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bioRxiv - Microbiology 2020Quote: All the templates required for the assay were labelled with [γ-32P] ATP using T4 DNA ligase (NEB) at room temperature for 1 h ...
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bioRxiv - Immunology 2020Quote: ... The nested PCR assays were run using the NEB enzyme Q5® Hot Start High-Fidelity (NEB, M0491) as follows ...
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bioRxiv - Biochemistry 2023Quote: ... RNA capping enzyme assays were performed in 10 μL of 1x RNA capping buffer (New England Biolabs, Inc.) containing 0.5 μM substrate RNA ...
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bioRxiv - Genomics 2022Quote: ... and ribosomal RNAs were depleted using ribosomal RNA depletion kit (rRNA Depletion Kit, NEB). Ribosomal depleted RNA was used to make the RNAseq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid mini-prep kit and PCR DNA clean-up kits were provided by NEB.
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bioRxiv - Developmental Biology 2021Quote: ... using HiScribe Sp6 kit (NEB) instead of T7.
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bioRxiv - Neuroscience 2021Quote: ... rRNA depletion kit (NEB #E6310), and Ampure XP beads (Beckman #A63881) ...
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bioRxiv - Microbiology 2020Quote: ... the PNGase kit from (NEB) was used per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... NEBNext rRNA Depletion kit (NEB) was used to remove bacterial and eukaryotic rRNA from total RNA isolated from pooled samples ...
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bioRxiv - Genetics 2022Quote: ... HiFi Assembly Cloning Kit (NEB) to insert the LYP1 homology sequence into the BamHI/EcoRV digest of Addgene plasmid #35121 to create the final backbone plasmid BFA0190 (Supplementary Figure 10) ...