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Citations for New England Biolabs :
1 - 50 of 55 citations for Simvastatin EP Impurity A Sodium Salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... pYES2sh was first linearized with the restriction enzymes NotI and EcoRI and amplified Ep-CoV2 or Ep-CoV2™ DNA fragments was inserted into the vector backbone using NEBuilder® HiFi DNA Assembly (NEB, Ipswich, MA, USA). The viral potassium channel KcvPBCV-1 was used as a positive control in the yeast complementation assay [36] ...
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bioRxiv - Cell Biology 2023Quote: ... Free nucleotides were purchased as 100mM stocks of dATP or dUTP as sodium salts in ultrapure water (NEB) and stored at -20°C ...
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bioRxiv - Bioengineering 2021Quote: ... EP-PCR was performed using Taq polymerase and the standard Taq buffer (New England Biolabs) with imbalanced dNTP (0.2 mM dATP ...
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bioRxiv - Neuroscience 2020Quote: ... Trimmed gel pieces were transferred to an EP tube and digested with Proteinase K (NEB, P8107) at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... Random mutagenesis was performed using Error-Prone PCR (EP-PCR) with Taq DNA polymerase (New England Biolabs). PCR products and restriction digestion products were purified using a GeneJET gel extraction kit (ThermoFisher) ...
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bioRxiv - Genetics 2020Quote: ... sodium orthovanadate (NEB, 1mM), and sodium fluoride (NEB ...
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bioRxiv - Physiology 2022Quote: ... sodium orthovanadate (NEB, 1mM), sodium fluoride (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 400 U of Salt-T4 DNA Ligase (NEB). All reactions were then incubated at 37 °C 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 8 U/μl Salt-T4 DNA ligase (NEB #M0467)) ...
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bioRxiv - Physiology 2022Quote: ... sodium fluoride (NEB, 1 mM), and sodium butyrate (100uM) ...
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bioRxiv - Pathology 2021Quote: ... The amplified Ep-CoV2 DNA fragment was then inserted into the vector backbone using NEBuilder® HiFi DNA Assembly (NEB, Ipswich, MA, USA). For Western blot analysis ...
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bioRxiv - Pathology 2021Quote: ... The DNA fragment encoding the desired FLAG-TEV tag was generated via PCR using primer pair 2 (table 1) and subsequently cloned into the NdeI restriction site of pET24Δlac/Ep-CoV2 using NEBuilder® HiFi DNA Assembly (NEB, Ipswich, MA, USA).
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bioRxiv - Molecular Biology 2020Quote: ... 2 mM Vanadate (Sodium Orthovanadate, NEB, pre-incubated for ten minutes at 95 °C to dissociate Vanadate oligomers ...
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bioRxiv - Genetics 2020Quote: ... and sodium fluoride (NEB, 1 mM). 150uL of ice cold buffer was added to heads followed by immediate homogenization with a motorized pestle for 10 seconds on ice ...
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bioRxiv - Cell Biology 2020Quote: ... or sodium orthovanadate (Na3VO4, 1 mM; NEB) to test dephosphorylation of CatSper1 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... 5 mM sodium orthovanadate (New England Biolabs), and 10 mM sodium fluoride (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... containing 1 mM sodium orthovanadate (NEB, # P0758S). The membrane from the transwell inserts was cut using forceps and placed in 300 µL of 1X cell lysis buffer (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... phosphatase inhibitors (Sodium Orthovanadate, New England BioLabs, P0758 ...
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bioRxiv - Cancer Biology 2022Quote: ... Sodium Orthovanadate (New England Biolabs, Cat. no. p0758s), Pepstatin (Cat ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 0.5% sodium dodecyl sulfate) and Proteinase K (NEB). Reactions were incubated at room temperature overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and sodium fluoride (New England Biolabs; item # P0759). Western blotting was performed using a sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... Sodium Orthovanadate (Vanadate) was from New England BioLabs (NEB), catalase and hydrogen peroxide (30 % ...
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bioRxiv - Biophysics 2021Quote: ... and Sodium Orthovanadate (New England BioLabs, Ipswich, MA, USA). Tissue was homogenized using an electric homogenizer ...
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bioRxiv - Genomics 2020Quote: ... 0.5% Sodium deoxycholate and 1.1% Murine Rnase Inhibitor (M0214L, NEB). Cells were allowed to lyse for 15 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM sodium vanadate (New England Biolabs, Ipswich, MA, #0758s); 50 mM sodium fluoride (Sigma ...
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bioRxiv - Biophysics 2022Quote: For inhibiting motors using Na3VO4 (sodium orthovanadate or ‘vanadate’) (NEB), vanadate was added to extract at a final concentration of 0.5 mM.
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bioRxiv - Immunology 2022Quote: ... and phosphatase inhibitors (sodium orthovanadate; New England Biolabs, Ipswich, MA). Proteins were resolved by SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... and 5 mM sodium fluoride (New England Biolabs, Ipswich, MA) and 1 mM sodium orthovanadate (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... and 1 mM sodium orthovanadate (New England Biolabs, Ipswich, MA) to inhibit phosphatases ...
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bioRxiv - Biophysics 2022Quote: Nucleosome reconstitution was performed by salt dialysis on biotinylated DNA (pKYB1 vector; New England Biolabs) without any nucleosome positioning sequence ...
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bioRxiv - Microbiology 2021Quote: ... RNase I (6xHis) or refolded RNase I-ACE2NTD (6xHis) in a high salt buffer (NEB buffer 3 ...
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bioRxiv - Molecular Biology 2020Quote: ... Nucleosomes were reconstituted with a salt dilution method according to manufacturer’s instructions (New England BioLabs) with a slight modification ...
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bioRxiv - Pathology 2020Quote: ... Heat mediated antigen retrieval pretreatment using 10mM Sodium Citrate buffer (Vectors Biolabs) was used ...
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bioRxiv - Cell Biology 2024Quote: ... and phosphatase inhibitors sodium orthovanadate (New England Biolabs, Ipswich, MA, USA; item # P0758) and sodium fluoride (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... The holder allowed immersion of the cochlea and the middle ear in oxygenated (95 % O2, 5 % CO2) cell culture medium (Minimum Essential Medium with Earle’s balanced salts, SH30244.FS Nordic Biolabs). The bone of the bulla was removed gently with bone cutters which exposed the middle ear and the basal turn of the cochlea ...
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bioRxiv - Microbiology 2020Quote: ... was isolated via ammonium acetate salt precipitation if greater than 1.5 × 106 cells were recovered or using the Monarch Genomic DNA Purification kit (NEB) if fewer per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... by gradient salt dialysis in the presence of 0.2 mg/ml nuclease-free bovine serum albumin (BSA, New England Biolabs). Quality of reconstitution was assessed by MNase and chromatosome stop assays (Figure 2-figure supplement 1D&E).
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bioRxiv - Microbiology 2021Quote: ... then directly resuspended in 1× SDS (sodium dodecyl sulphate) sample buffer (New England BioLabs) per 1 ml of OD600 of 1.0 cells in 200 μl sample buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... TIS11B protein-RNA complexes were immunoprecipitated from 1 ml of crosslinked lysate and washed with high salt and PNK buffer (NEB). RNA was repaired by 3′ dephosphorylation and ligated to L3-IR adaptor on beads ...
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bioRxiv - Biophysics 2024Quote: ... 55 The samples were mixed with 6 × purple loading dye without sodium dodecyl sulfate (NEB) and with 10 × TBE to make a 1 × solution ...
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bioRxiv - Biochemistry 2020Quote: ... beads were resuspended in 15 μL low salt buffer and the DNA was released by adding 600 units of Micrococcal nuclease (NEB M0247S) for 5 minutes with shaking (1250 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-miRNAs were purified on denaturant polyacrylamide gel and long pri-miR-K10/12 derived transcripts (up to ∼3 kb) were salt purified using Monarch® PCR and DNA cleanup kit (New England BioLabs). After acidic phenol extraction and ethanol precipitation ...
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bioRxiv - Neuroscience 2021Quote: ... 50mM Sodium Butyrate) and incubated in a 37C water bath with 2 microliters Micrococcal Nuclease enzyme (NEB) for eight minutes ...
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bioRxiv - Biophysics 2021Quote: ... of 0.1M sodium hydroxide: 0.02M 2-(n-morpholino) ethanesulfonic acid (MES) or 1X NEB DNase I reaction buffer (NEB B0303S ...
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bioRxiv - Cell Biology 2024Quote: ... in a reaction solution containing 50 mM sodium phosphate (pH 7.5) and 1% NP-40 (B2704S, NE BioLabs) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were brought up to 50μl and subsequently digested with 1μl of Nuclease P1 in 50mM sodium acetate buffer (New England Biolabs, M0660) for 2h at 45°C ...
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bioRxiv - Microbiology 2022Quote: ... RNAs were precipitated in cold ethanol and 0.3 M of sodium acetate and dephosphorylated using Calf-intestinal alkaline phosphatase (New England Biolabs), according to manufacturer protocol ...
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bioRxiv - Neuroscience 2024Quote: ... the gel was immersed in 0.3 M sodium acetate solution containing 20 Unit RNase inhibitor (New England Biolabs, M0314L) overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... a 50 mM stock solution of pervanadate was prepared fresh by mixing equal volumes of 100 mM sodium orthovanadate (New England Biolabs) and 100 mM hydrogen peroxide ...
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bioRxiv - Neuroscience 2022Quote: 4 µl procainamide-labeled glycans were combined with 1 µl 10x sodium acetate – Ca2+ buffer (Glycobuffer 1, New England Biolabs), 1 µl 10x BSA (diluted 1:10 with Milli-Q water from a 100X stock ...