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Citations for New England Biolabs :
301 - 350 of 1821 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... PCR products were purified by gel extraction (NEB, T1020) and sequenced on a NextSeq 500 (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified plasmids were digested stepwise with SacI (NEB, R3156S) to prevent exoIII digestion on the arrest peptide side and then with AvrII (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... further purified (Monarch RNA Cleanup Kit, NEB, Ipswich, MA) and cleaned of genomic DNA (Ambion Turbo DNA-free kit ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were purified by gel extraction (NEB, T1020) and sequenced on a NextSeq 500 (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The complexes were further purified on amylose resin (NEB). Fractions containing protein were pooled and diluted in 3 volumes of amylose buffer (25 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was first purified on amylose resin (NEB) in a buffer containing 20 mM Tris pH 8 ...
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bioRxiv - Genetics 2023Quote: ... then purified using a standard Monarch (New England Biolabs) column purification procedure ...
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bioRxiv - Biochemistry 2023Quote: ... The assembled pentamer was purified on amylose beads (NEB), after which the MBP and His6 tags were cleaved using TEV protease incubation overnight ...
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bioRxiv - Biophysics 2023Quote: ... The modified RNA was subsequently column-purified (NEB # T2030), 2 pmol were digested to single nucleosides using the Nucleoside Digestion Mix (NEB # M0649) ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was purified using a PCR purification kit (NEB) following manufacturer instructions.
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bioRxiv - Genomics 2023Quote: ... Amplicons were purified with the Monarch PCR kit (NEB), digested with MfeI-HF and SphI-HF (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purified RNA was digested using 4U DnaseI (NEB) in a 50μL reaction incubated at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... products were purified using an RNA purification kit (NEB), absorption at 260 nm was determined and length of the RNA product was analyzed on a 1 % agarose gel.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were subsequently purified on amylose resin (E8021L, NEB): 3 ml resin per column and 45 ml of extract ...
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bioRxiv - Biochemistry 2024Quote: ... and purified with Monarch PCR & DNA cleanup kit (NEB). PURExpress in vitro translation reaction was assembled in RNase-free tubes according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... polyadenylated RNA was purified with oligo(dT) beads (NEB) from 15 µg of total RNA extracted from P24 testes ...
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bioRxiv - Molecular Biology 2024Quote: ... then purified using a standard Monarch (New England Biolabs) column purification procedure ...
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bioRxiv - Molecular Biology 2024Quote: ... then purified using a standard Monarch (New England Biolabs) column purification procedure ...
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bioRxiv - Developmental Biology 2024Quote: ... Reactions were purified with a PCR purification kit (NEB). Approximately 120–150 ng of DNA was used as a template for a T7 in vitro transcription (IVT ...
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bioRxiv - Developmental Biology 2024Quote: ... and purified using the Monarch RNA Cleanup Kit (NEB) and stored at −80 °C before use ...
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bioRxiv - Microbiology 2024Quote: ... gel purified with Monarch DNA Gel Extraction Kit (NEB), and ligated with T4 DNA ligase (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... and further purified by Monarch RNA purification columns (NEB) and resuspended in a TE buffer at the desired concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA was then 5’ end labeled in 24μL PNK wash buffer with 16μL of 1x PNK buffer (NEB) containing 150μCi of gamma P32-ATP ...
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bioRxiv - Molecular Biology 2019Quote: ... The 5’-monophosphate standard was generated by treating the 5’-triphosphate/3’-FAM-labeled 25mer with Apyrase (NEB). The 5’-hydroxyl standard was generated by treating the 5’-triphosphate/3’-FAM-labeled 25mer with calf intestinal phosphatase (NEB) ...
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bioRxiv - Biochemistry 2022Quote: 3’-PUA-DNA was generated by incubation of 1 μM 5’-FAM-labeled AP-DNA (FAM_U_35) with 20 U EndoIII/Nth (NEB) in Buffer B pH 7.0 at 37 °C for 60 m ...
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bioRxiv - Biophysics 2019Quote: ... Biotin- and digoxygenin-labeled handles were amplified with a non-proofreading Taq DNA polymerase (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biophysics 2019Quote: ... Biotin- and digoxygenin-labeled handles were amplified with a non-proofreading Taq DNA polymerase (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Genomics 2019Quote: ... Nicked DNAs were labeled at 72°Cfor 60 minutes using 15 U Taq DNA Polymerase (New Engand Biolabs) in 1× each Labeling Buffer and Labeling Mix (Bionano Genomics) ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... was detected by addition of 1 nM Europium-labeled anti-p53 phosphoserine 15 antibody (New England Biolabs/ Cisbio) and 40 nM streptavidin-conjugated APC (Prozyme ...
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bioRxiv - Immunology 2020Quote: SNAP-ASC-CARD and CASP1-CARD-SNAP monomers that were labeled with Alexa Fluor 647 (New England Biolabs) were co-incubated with CARD domain only filament seeds (CARD8-CARD without SNAP ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA probes (Extended Table 1) at 500 nM were 5’-[32P]-labeled using T4 Polynucleotide Kinase (NEB) and hybridized to the membrane overnight at 37°C in PerfectHyb™ Plus Hybridization Buffer (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... this extension could be radioactively labeled by filling in the overhangs using 32P-dCTP and Klenow enzyme (NEB). Sequences of the +-strand oligonucleotides used are described in Table 1.
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bioRxiv - Cell Biology 2023Quote: ... Snap-tagged human β1AR and β2AR constructs were labeled with Snap-cell 647 SiR (New England Biolabs, S9102S) at 1:1000 concentration for 20 min at 37°C in DMEM without phenol red supplemented with 30 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... A stock solution of these oligos (1μl of a 10μM) was 5’-end-labeled using T4PNK (NEB®) and 5μl of ATP ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The purified dsDNA product was end-repaired (New England Biolabs) and A-tailed (Klenow fragment ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified PCR product was digested with Hpy188I (New England Biolabs) for 2 hours at 37 °C to generate single dT 3’ overhangs ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was purified with mini-prep kits from NEB. Screening PCRs were performed using DreamTaq polymerase (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged complexes were further purified on amylose resin (NEB). The core complex without MBP was instead further purified on Anti-Flag M2 affinity resin (Sigma) ...
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bioRxiv - Genomics 2020Quote: ... RNA was purified using a Monarch RNA purification kit (NEB). RNA quality was evaluated with an Agilent Tape station ...
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bioRxiv - Genomics 2020Quote: ... Nascent RNA was purified by binding streptavidin beads (NEB, S1421S) before and in between the following procedures ...
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bioRxiv - Immunology 2021Quote: Purified recombinant CV3-25 IgG was mixed with LysC (NEB) at a ratio of 1μg LysC per 10mg of IgG and incubated at 37°C for 18h with nutation ...
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bioRxiv - Developmental Biology 2019Quote: ... purified with the Monarch PCR and DNA Cleanup kit (NEB), and then dialyzed with 0.5X TE buffer using a 0.025 μm VSWP membrane (Millipore ...
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bioRxiv - Immunology 2019Quote: ... purified using the Monarch Gel Extraction kit (New England Biolabs), and cleaned with MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Genomics 2019Quote: ... This duplex was purified and assembled using Gibson mix (NEB) with p426-crRNA-CAN1.Y that had been cut with NheI and Acc65I to replace the CAN1.Y guide target region with a stuffer fragment that could be released by BaeI (NEB ...
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bioRxiv - Immunology 2019Quote: ... The PCR product was purified and assembled into BsmBI (NEB) digested vector backbones in a Gibson reaction (NEB) ...
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bioRxiv - Cell Biology 2019Quote: ... The protein was purified on amylose resin beads (E8021S, NEB) according to manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Barcoded amplicons were purified with SPRIselect beads (New England Biolabs) and sequenced with a MiSeq kit (Illumina).
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bioRxiv - Immunology 2021Quote: ... Barcoded libraries were prepared from purified mRNA (New England Biolabs, #E7530S ...
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bioRxiv - Bioengineering 2021Quote: ... RNA was purified using a Monarch RNA Cleanup Kit (NEB) and quantified on a Nanodrop spectrophotometer (Thermo).