Labshake search
Citations for New England Biolabs :
201 - 250 of 2046 citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... The SNAP-tag sequence was amplified using pSNAPf vector (#N9183, NEB) and inserted into the AscI and KpnI sites after UBQ10 promoter in the UBQ10pro-p4p1r entry vector ...
-
bioRxiv - Systems Biology 2022Quote: ... BCKDK was purified by its MBP tag on amylose resin (NEB).
-
bioRxiv - Cell Biology 2022Quote: ... the GST-6xHis-tag was removed by TEV protease (#P8112S, NEB) and dArc1 and dArc2 proteins were further purified by size exclusion chromatography using Superdex S200 16/600 (GE Healthcare Life Sciences).
-
bioRxiv - Cell Biology 2022Quote: ... EGFP was replaced by a ybbR-tag using Q5 mutagenesis (NEB #). For kif5b ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Original SNAP tag and Nanoluciferase sequences were from NEB (Hitchen UK) and Promega (Southampton UK ...
-
bioRxiv - Biophysics 2022Quote: ... Three are commercially available orthogonal self-labeling approaches: SNAP-tag (NEB), CLIP-tag (NEB) ...
-
bioRxiv - Neuroscience 2022Quote: ... and CLIP-tag ligands (CLIP surface 647 - BC 647 [NEB, S9234S]) at final concentrations of 1 μM in 0.3% PBT ...
-
bioRxiv - Molecular Biology 2021Quote: ... were then combined with 10 μM CLIP-Biotin (New England Biolabs) and rotated (end-over-end ...
-
bioRxiv - Genomics 2019Quote: ... Biotin labelled DNA was pulled down on Streptavidin Dynabeads (NEB, S1420S). After DNA repair and 3’ A addition ...
-
bioRxiv - Genomics 2019Quote: ... Biotin labelled DNA was pulled-down on Streptavidin Dynabeads (NEB, S1420S). After DNA repair and 3’ A addition ...
-
bioRxiv - Genomics 2023Quote: ... dTTP and biotin-dATP using Klenow DNA polymerase I (NEB M0212L), followed by in situ proximity ligation at room temperature overnight ...
-
bioRxiv - Plant Biology 2020Quote: ... Table S1) were used to amplify GmSALT3 cDNA with Phusion® High-Fidelity DNA Polymerase (New England Biolabs) using 35 cycles (98 °C 30 s ...
-
A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... using the primers outlined in Table S1 and Q5 Hot-start high fidelity polymerase (New England Biolabs M0494S). PCR products were extracted from standard agarose gels using the QIAquick Gel Extraction Kit (Qiagen 28704 ...
-
bioRxiv - Developmental Biology 2023Quote: Plasmids (otxG:Tet-ON 3G; TRE3Gs:Cas9; TRE3Gs:Kaede; U6:sg2; Table S1) were built by Gibson cloning (NEB, Cat# E2611) following standard protocols ...
-
bioRxiv - Biochemistry 2020Quote: ... Tag introduction and mutagenesis was achieved through Gibson assembly (New England Biolabs) to generate SMC1-His ...
-
bioRxiv - Microbiology 2022Quote: ... Affinity tags were introduced by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) into the expression vectors encoding Rae1 and Nup98 using primers in Table 1 ...
-
bioRxiv - Microbiology 2021Quote: ... Then the His-tag was cleaved by incubation with TEV protease (NEB) for 18 h at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... and the His6-MBP tag was subsequently recaptured using amylose resin (NEB). The flow-through was loaded onto a Q-HP anion exchange column (Cytiva ...
-
bioRxiv - Cell Biology 2023Quote: ... and subsequently the 6xHIS tag was cleaved using the TEV Protease (NEB) by adding TEV enzyme assuming all protein isolate was 6xHIS tagged based on the activity of the TEV protease ...
-
bioRxiv - Biochemistry 2023Quote: ... and SNAP tags were stained with SNAP-Cell® 505-Star (NEB). HaloTag® Ligands TMRDirect were added to medium to a final concentration of 50nM and incubated overnight ...
-
bioRxiv - Cancer Biology 2023Quote: ... The 3XFLAG tag was replaced by Q5® site-directed mutagenesis (NEB) with either a Myc-tag or Strep-tag®II ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins with MBP-tag were loaded onto amylose resin (New England Biolabs) after elution from Ni-NTA ...
-
bioRxiv - Biophysics 2022Quote: ... rk430-mScarlet-SNAP (7 μM monomer) was incubated with benzylguanine-biotin (NEB) in a 4 to 1 molar ratio at room temperature for 15 minutes ...
-
bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs). Regularly ...
-
bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs).
-
bioRxiv - Cell Biology 2023Quote: ... HEK293 libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat. #E7770) or NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (#E7760) ...
-
bioRxiv - Immunology 2021Quote: ... The kRSV-DB1-QUAD plasmid and spike insert were digested with the enzymes AatII and SalI (NEB) and ligated with T4 DNA ligase (NEB ...
-
bioRxiv - Biophysics 2021Quote: ... Complexes were prepared by incubating spike ectodomain preparations with either ACE2 (residues 18–615, New England Biolabs), VH ab8 ...
-
bioRxiv - Microbiology 2022Quote: ... Spike variants with single-site residue substitutions were generated using Q5® High-fidelity DNA polymerase (NEB)-based site-directed mutagenesis.
-
bioRxiv - Neuroscience 2022Quote: ... ∼150ng of sheep genomic DNA was digested overnight with the restriction enzyme MspI (New England Biolabs, Ipswich, MA, USA). The DNA was then purified with AMPure XP magnetic beads (Beckman Coulter ...
-
bioRxiv - Genetics 2021Quote: ... primers P1 and P2 (Table S1) were used with the Q5 Hot Start High-Fidelity 2X Master Mix (NEB). To ensure coverage for each sample ...
-
bioRxiv - Plant Biology 2021Quote: ... We amplified the corresponding cDNAs using the primers on Table S1 and Q5® High-Fidelity DNA Polymerase (NEB). The PCR fragments were cloned into pENTR-D Topo vector and sequenced.
-
bioRxiv - Cell Biology 2022Quote: ... PCR was performed using pBluescript SK+ CPES_V5 clone as template with appropriate sense and antisense primers (Table S1) and Phusion polymerase (NEB). The PCR product was digested with DpnI to remove templates followed by transformation into DH5α competent cells ...
-
bioRxiv - Cell Biology 2022Quote: ... PCR was performed using pBluescript SK+ CPES_V5 clone as template with appropriate sense and antisense primers (Table S1) and Phusion polymerase (NEB). The PCR product was digested with DpnI to remove templates followed by transformation into DH5α competent cells ...
-
bioRxiv - Bioengineering 2022Quote: ... custom synthesized oligonucleotides encoding the sgRNA spacer sequences (IDT) (Supplementary Table S1) were phosphorylated by T4 polynucleotide kinase (NEB) for 30 min at 37°C and subsequently annealed in a thermocycler (5 min at 95° ...
-
bioRxiv - Immunology 2020Quote: ... Ighγ1 heavy-chain sequences were amplified in a nested PCR using primers for the Ighγ1 constant region together with a specific primer for VH186.2 (Mayer et al., 2017)(Table S1) and using high-fidelity Q5 polymerase (NEB). Amplified products were cloned (CloneJET PCR Cloning Kit ...
-
bioRxiv - Genetics 2020Quote: ... PCR to amplify the barcode for Illumina sequencing was performed on 2.5 µL of isolated DNA (primers listed in Table S1) with Q5 polymerase (NEB), following manufacturer’s instructions with between 25-35 cycles ...
-
bioRxiv - Cancer Biology 2022Quote: ... Datafile S1) and introduced into the NheI/BamHI restriction sites of pAAV-MCS2 by Gibson assembly (New England Biolabs). N-BioTAP and N-PrA cassettes targeted the first ATG of the MYC gene ...
-
bioRxiv - Molecular Biology 2023Quote: ... pairs of complementary DNA oligos (table S1) were annealed and phosphorylated with T4 polynucleotide kinase [New England Biolabs (NEB)] as previously described (3) ...
-
bioRxiv - Genomics 2023Quote: ... Datafile S1) and introduced into the NheI/BamHI restriction sites of pAAV-MCS2 by Gibson assembly (New England Biolabs). The N-BioTAP cassette was targeted to the first ATG of the MYC gene ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells expressing the SNAP-tag and stained with SNAP-cell 647-SiR (NEB) were excited with a white light laser at an excitation wavelength of 633 nm ...
-
bioRxiv - Cell Biology 2019Quote: ... the sequence encoding SNAP-tag was PCR amplified from pSNAPf (New England BioLabs) with primers to append flanking BamHI and NotI restriction sites (Forward ...
-
bioRxiv - Biophysics 2023Quote: From the H6-RapA expression plasmid and pSNAP-tag(T7) plasmid (NEB #101137), we constructed a plasmid pKI1 (Addgene #199118 ...
-
bioRxiv - Plant Biology 2024Quote: ... 0.4 μL Buffer Tag DNA polymerase (5 U/μL, NEB, Cat. No. B9004S), 1.6 μL dNTP (TAKARA ...
-
bioRxiv - Biochemistry 2023Quote: ... GST-tag HP1⍺ΔC construct was generated by site-direct mutagenesis kit (NEB) by deleting the amino acids (number 169 –181 ...
-
bioRxiv - Microbiology 2022Quote: ... resulting in a linearized vector into which synthetic variant spike genes can be assembled using Gibson Assembly (NEB). The Gibson Assembly reaction was then transformed into TransforMax™ EPI300™ Electrocompetent E ...
-
bioRxiv - Microbiology 2022Quote: ... PCR of a spike gene fragment was performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) and the following primers ...
-
bioRxiv - Synthetic Biology 2020Quote: ... SNAP reagents (BG-649, BG-fluorescein and BG-biotin) were obtained from NEB. Electrocompetent cells were used in all transformations.
-
bioRxiv - Cell Biology 2021Quote: ... and biotin-pupE labelled proteins were enriched by streptavidin magnetic beads (NEB, S1420S) and identified by mass spectrometry analysis (Liu et al. ...
-
bioRxiv - Biophysics 2023Quote: ... The biotin-modified DNA was digested using a KasI restriction enzyme (NEB #R0544S) that produced two fragments of 24 kb and 24.5 kb ...