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Citations for New England Biolabs :
201 - 250 of 2152 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... or Bam HI and Xho I (New England BioLabs, MA). The column isolated DNA fragments were ligated into our modified pFastBac1 plasmid having 6xHis tag at C-terminus ...
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bioRxiv - Molecular Biology 2020Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... Followed by subcloning the PCR amplicon in Bam-HI (NEB) restriction digested pCW57-tGFP-2A-MCS plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... using NEBuilder Hi-Fi Assembly (New England Biolabs, Cat. #E2621). The pMB1052 construct was subsequently cut using BspDI and EcoRV (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... The Hi-Scribe T7 High Yield RNA synthesis kit (NEB) was used to generate RNA transcripts at 37°C for 16 hours ...
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bioRxiv - Systems Biology 2024Quote: ... using the NEBuilder Hi-Fi Assembly kit (New England Biolabs). To ensure representation of all variants in the population ...
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bioRxiv - Cancer Biology 2023Quote: ... cut with hi-fidelity EcoRI and BamHI (New England Biolabs), and gel purified ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragment encoding SNAP-tag was amplified from the pSNAP-tag (m) vector (a gift from New England Biolabs & Ana Egana, Addgene #101135), and then inserted into the pDisplay vector (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Fusions to the SNAP-tag (New England Biolabs, Beverly, MA) were expressed from plasmid pAGT-Xpress ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... SNAP-tag® ligand Blue 430 (New England Biolabs, S9109S) was applied for 30 min at a final concentration of 2 µM ...
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bioRxiv - Biophysics 2021Quote: ... The His6-MBP tag was recaptured using amylose resin (NEB). The flow-through was loaded onto a Q-HP anion exchange column (Cytiva ...
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bioRxiv - Systems Biology 2022Quote: ... The SNAP tag insert was derived from pSNAPf (NEB, N9183S) and the FRB-Fis1 insert was derived from pC4-RhE-FRB-Fis1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... SNAP-tag comes from pSNAPf-ADRβ2 control plasmid (NEB, #N9184). SNAP-CB2-TwinStrep-1D4 ...
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bioRxiv - Molecular Biology 2023Quote: ... for Strep-tag II pull-downs and Amylose resin (NEB) for MBP pull-downs) ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... the MPB tag was cleaved overnight using Factor Xa (NEB) in dialysis buffer and loaded again on an MBP-Trap HP column ...
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bioRxiv - Cell Biology 2024Quote: ... pSNAPf vector containing SNAP-tag was purchased from NEB (N9183S) and optimised to Drosophila codon suing Genewiz (USA ...
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bioRxiv - Biochemistry 2021Quote: The Bam35 genomic DNA was used to amplify the gene 2 flanked by KpnI and BamHI sites by PCR with B35SSB_FW_KpnI and B35sSB_RV_BamHI primers (Table S1) and Vent DNA Polymerase (New England Biolabs). The digested PCR product was cloned into a pET-52b(+ ...
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bioRxiv - Physiology 2022Quote: ... sense and anti-sense oligo DNAs (IDT) (Table S1) were phosphorylation using T4 Polynucleotide Kinase (NEB) at 37 °C for 30 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... we use Western Blot to detect spike protein with/without treatment of PNGase F (NEB). 100 ng particles were incubated with Glycoprotein Denaturing Buffer at 98°C for 10 min ...
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bioRxiv - Biochemistry 2021Quote: The VoC RBD mutations were introduced by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs). Successful cloning was confirmed by Sanger sequencing (Genewiz ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The two fragments were assembled using HI-FI assembly (NEB E5520S). To construct the Prcan-1-R1∷mCherry and Prcan-1-R2∷mCherry plasmids ...
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bioRxiv - Immunology 2021Quote: ... The fragments were assembled using Hi-Fi DNA Assembly Mix (NEB). For the DonorgRNA vector ...
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bioRxiv - Genomics 2021Quote: ... and 25 µl 2x NEBNext Hi-Fi PCR mix (NEB, USA) per reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10 μg/mL) and apyrase (25 mU/mL, NEB); the suspension was incubated for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nb35–His (10 µg/ml) and apyrase (25 mU/ml, NEB). The suspension was incubated for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: His-MBP-Fbp17SH3 was precipitated by amylose beads (NEB; cat#E8021L). After purification ...
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bioRxiv - Plant Biology 2022Quote: ... and MBP-HIS-GFP proteins were purified with amylose resin (NEB) and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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bioRxiv - Cell Biology 2023Quote: ... For all Gibson Assemblies the NEB Hi-Fi Assembly Mastermix (NEB) was used ...
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bioRxiv - Genetics 2023Quote: ... To facilitate homology directed assembly (Gibson or NEB Hi Fi assembly), 30bp homology with the preferred SEED cassettes were included at each site of the BsaI sites ...
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bioRxiv - Synthetic Biology 2023Quote: ... For his purpose pEKEx2 was first linearized using SacI (NEB, USA). The gene for mCherry was amplified by PCR using the primer pairs low_mCherry_fw and low_mCherry_rev (Table S1 ...
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bioRxiv - Microbiology 2021Quote: ... plates were immunostained using a monoclonal antibody against SARS-CoV2 nucleoprotein (Creative-Biolabs; NP1C7C7) at a dilution of 1:1000 followed by 1:5000 anti-mouse IgG monoclonal antibody and was developed using KPL TrueBlue peroxidase substrate for 10 minutes (Seracare ...
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bioRxiv - Microbiology 2022Quote: ... pCAGGS.SARS-CoV-2_SΔ19_fpl_mNG2(11)_opt was generated through gibson assembly (NEBuilder, New England Biolabs) of a pCAGGS vector backbone cleaved using EcoRV-HF and HindIII-HF (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: pCAGGS.SARS-CoV-2_SΔ19_fpl_mNG2(11)_opt was generated using NEBuilder DNA assembly (New England Biolabs) of a pCAGGS vector backbone cleaved using EcoRV-HF and HindIII-HF (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... and tRNA-Gly-CCC (Table S1) were radioactively labeled with gamma-32P-ATP using T4 PNK (NEB). Pre-hybridization and hybridization were performed in OligoHyb Buffer (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
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bioRxiv - Plant Biology 2021Quote: ... digested alongside pYL156 with restriction enzymes as listed in Supplementary Table S1 (enzymes from New England Biolabs), and ligated with Promega T4 DNA ligase (Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
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bioRxiv - Biophysics 2022Quote: ... and degradation tags were amplified by PCR with Q5 polymerase (NEB), gel extracted (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... The SNAP-tag sequence was amplified using pSNAPf vector (#N9183, NEB) and inserted into the AscI and KpnI sites after UBQ10 promoter in the UBQ10pro-p4p1r entry vector ...
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bioRxiv - Systems Biology 2022Quote: ... BCKDK was purified by its MBP tag on amylose resin (NEB).
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bioRxiv - Cell Biology 2022Quote: ... the GST-6xHis-tag was removed by TEV protease (#P8112S, NEB) and dArc1 and dArc2 proteins were further purified by size exclusion chromatography using Superdex S200 16/600 (GE Healthcare Life Sciences).
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bioRxiv - Cell Biology 2022Quote: ... EGFP was replaced by a ybbR-tag using Q5 mutagenesis (NEB #). For kif5b ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Original SNAP tag and Nanoluciferase sequences were from NEB (Hitchen UK) and Promega (Southampton UK ...
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bioRxiv - Biophysics 2022Quote: ... Three are commercially available orthogonal self-labeling approaches: SNAP-tag (NEB), CLIP-tag (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... and CLIP-tag ligands (CLIP surface 647 - BC 647 [NEB, S9234S]) at final concentrations of 1 μM in 0.3% PBT ...
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bioRxiv - Genetics 2019Quote: ... Hi-C DNA was amplified using Index Primers set 1 (NEB, E7335S). The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... and the fragment was assembled using Hi-Fi DNA Assembly Mix (NEB) into the donor vector pADN171XS7 ...
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bioRxiv - Microbiology 2021Quote: ... His-MBP-MirA were first isolated using amylose bead affinity chromatography (NEB) in conditions specified by the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... The homology plasmids were assembled using HI-Fi DNA Assembly Mastermix (NEB) with four PCR-generated DNA fragments (see Table S2 for primers) ...