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Citations for New England Biolabs :
451 - 500 of 4485 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... coli (NEB C3020K). The transformed library was grown overnight in 50 mL of liquid Luria Broth (LB ...
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bioRxiv - Molecular Biology 2024Quote: ... coli (NEB, C30131). 10 mL overnight cultures were diluted into 1 mL LB medium with 50 µg/mL kanamycin (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... coli cells (NEB) and identified by colony PCR followed by Sanger sequencing.
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bioRxiv - Biochemistry 2024Quote: ... coli (NEB, C2527H) via autoinduction ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli DH5α (NEB). Escherichia coli strains and plasmids used are listed in Supporting Information ...
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bioRxiv - Biochemistry 2024Quote: ... coli PPIase (NEB), 5 U µL-1 T7 RNA polymerase (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... coli cells (NEB) were transformed with plasmids encoding β-gal RNC constructs and grown in ZYM-5052 autoinduction media 106 for 18 h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Coli (NEB, C2527I) were grown on LB (Luria-Bertani ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5a (NEB) was used for cloning ...
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bioRxiv - Genomics 2024Quote: ... coli (NEB #C3020). Briefly ...
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bioRxiv - Genetics 2024Quote: ... coli cells (NEB), with selection on LB agar plates using spectinomycin and ampicillin ...
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bioRxiv - Microbiology 2023Quote: ... coli (NEB, C2566) and transformed cells were plated on selective Kan LB agar plates and incubated overnight at 30 °C.
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bioRxiv - Microbiology 2024Quote: ... coli C2925 (NEB). Cloned gene expression was induced by inclusion of the indicated concentration of L-tryptophan (Trp ...
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bioRxiv - Bioengineering 2024Quote: ... coli T7SHuffle (NEB). pET28a and pETDuet constructs were transformed into chemically competent E ...
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bioRxiv - Physiology 2023Quote: ... coli (C2987H, NEB) by heat shock at exactly 42°C for exactly 30 seconds ...
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bioRxiv - Biochemistry 2023Quote: ... VCE or FCE::T7RNAP fusion and 5 U/μL vaccinia cap 2′-O-methyltransferase (New England Biolabs). Reactions were carried out at indicated temperatures for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant fusion proteins were purified via affinity chromatography from bacterial extracts using amylose resin (New England Biolabs) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... A small fraction of the refolded MBP-ACE2NTD fusion protein was further purified by amylose magnetic beads (NEB) according to the protocol provided by the manufacturer.
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bioRxiv - Genetics 2023Quote: N-terminal GST-fusion TF DBD proteins were expressed using the PURExpress in vitro transcription/translation kit (NEB) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 S R403T and RaTG13 S T403R/T403A mutant plasmids were generated using Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... E protein glycosylation status was determined by treating RVP lysates with PNGase F (Cat#P0704S, New England Biolabs) for 3 hours at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µL of Lambda Protein Phosphatase (NEB, P0753S) was added to aliquots 3 and 4 (PPase only) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 µL of λ protein phosphatase (NEB P0753S) was added and incubated at 37°C for 30 minutes and then placed on ice ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 2 mM CaCl2 and the TRX-6His-S-tag was cleaved overnight at RT with enterokinase protease (NEB). The ET domain was further purified using Nickel-NTA resin (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The SNAP-tag sequence (NEB) was cloned into a β-cell-specific expression vector with a porcine INS gene promoter ...
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bioRxiv - Synthetic Biology 2022Quote: ... rabbit anti-SNAP-tag (NEB; P9310 ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Biochemistry 2022Quote: ... The N-linked glycans and the CBP tag were cleaved by endoglycosidase H (Endo H, ∼0.2 mg per 10–15 mg purified protein, New England Biolabs) and HRV-3C protease (∼2 mg per 10–15 mg purified proteins ...
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... histidine tags were cleaved with LysC (200 ng/mg of protein; two hours at room temperature; New England Biolabs), and for all others ...
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bioRxiv - Cell Biology 2024Quote: ... MBP tags that co-eluted with NTF2L proteins were purified by incubating with amylose resin (New England BioLabs, #E8021L) overnight at 4°C and collecting the flow through the following day ...
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bioRxiv - Immunology 2022Quote: HIV envelope plasmids were transformed and amplified in NEB-stable competent cells (NEB, C3040H). Pseudoviruses were produced by co-transfection of plasmids encoding various HIV-1 Envs together with NL4-3 ΔEnv or Q23-ΔEnv (1:3 ratio ...
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bioRxiv - Bioengineering 2021Quote: ... and cleavage of the fusion protein with enterokinase that were performed according to manufacturer’s instructions (amylose resin purification – according to pMAL Protein Fusion & Purification System, New England Biolabs, Ltd. ...
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bioRxiv - Neuroscience 2020Quote: ... To perform in vitro phosphorylation 5 μg of purified GST-NL2CT (WT or S714D) fusion protein was incubated with 2.5kUnits purified catalytic subunit of PKA (NEB) supplemented with 200 μM ATP at 30°C for the indicated time points.
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bioRxiv - Bioengineering 2020Quote: ... coli DH10B strain (NEB #C3019 New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... coli (New England BioLabs) to produce demethylated plasmid ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli DH5α cells (NEB).
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bioRxiv - Cell Biology 2020Quote: ... coli DH5α cells (NEB). The ‘601’ fragments were released from the plasmid by digestion with EcoRV (NEB) ...
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bioRxiv - Genetics 2021Quote: ... coli cells (NEB, C2987H) and plated on LB/ampicillin/IPTG/X-gal plates (according to pGEM-T Easy Vector System manufacturer’s instructions) ...
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bioRxiv - Microbiology 2019Quote: ... coli 70S ribosome (NEB). Lead acetate mediated cleavage was performed as described above ...
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bioRxiv - Biochemistry 2022Quote: ... coli NEBα (NEB, Canada) competent cells and were purified using NEB Monarch Miniprep Kits (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... coli DNA ligase (NEB) and DNA polymerase I (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli DNA ligase (NEB), and DNA Pol I (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... coli Topoisomerase I (NEB).
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bioRxiv - Molecular Biology 2020Quote: ... coli Ribonuclease H (NEB) or ddH2O in 1xRibonuclease H buffer (2 h ...
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bioRxiv - Cell Biology 2022Quote: ... coli cells (NEB # C2987) were transformed with the ligation product and plated following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... coli (New England BioLabs). E ...
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bioRxiv - Cell Biology 2019Quote: ... coli inorganic pyrophosphatase (NEB), 4 U/μl T7 RNA polymerase (NEB - pH 7.9 ...
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bioRxiv - Biochemistry 2019Quote: ... coli (New England Biolabs) with 1mM IPTG induction in a shake flask with 1L LB supplemented with 10mM Glucose ...
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bioRxiv - Microbiology 2019Quote: ... coli (New England Biolabs) by heat shock ...