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Citations for New England Biolabs :
201 - 250 of 590 citations for Respiratory Syncytial Virus A Glycoprotein G Mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 30 μg of Protein G magnetic beads (NEB #S1430) was washed with 250 μl of Immunoprecipitation Reaction Buffer (150 mM NaCl ...
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bioRxiv - Developmental Biology 2023Quote: ... then capped with m7G(5’)ppp(5’)G (NEB) and tailed with a poly(A ...
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bioRxiv - Neuroscience 2024Quote: ... 25-50 ul Protein G Magnetic Beads (NEB #S1430S) were incubated with 3ug GFP (D5.1 ...
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REEP4 is recruited to the inner nuclear membrane by ELYS and promotes nuclear pore complex formationbioRxiv - Cell Biology 2020Quote: ... The V5-tag and TEV cleavage site sequences were inserted into these plasmids based on Gibson assembly (NEB) using an oligo encoding for these features ...
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bioRxiv - Biochemistry 2019Quote: ... which followed digestion of affinity tag and deglycosilation by TEV protease and MBP-fusion endoglycosidase (New England Biolabs), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... The cleaved 6xHis-MBP tag was removed by incubating the solution with Ni-NTA magnetic beads (NEB; S1423) for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: SNAP-GCGR was detected with an anti-SNAP-tag rabbit polyclonal antibody (P9310S, New England Biolabs, 1/500) followed by goat anti-rabbit IgG H&L HRP (ab6271 ...
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bioRxiv - Plant Biology 2021Quote: ... The glutathione S-transferase (GST) tag was cleaved using Factor Xa Protease (New England Biolabs, Ipswich, MA, USA) in accordance with the manufacturer’s instruction.
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bioRxiv - Biophysics 2021Quote: ... The SNAP tag on Nup358min-zip and Nup358min was biotinylated with SNAP-biotin substrate (New England BioLabs, MA) as described99.
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bioRxiv - Biophysics 2019Quote: ... Purity and molecular weight were confirmed by staining with a SNAP-tag-reactive benzylguanine-Alexa 647 dye (NEB) and running the proteins on a 4-20% NuPAGE Bis-Tris gel ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 2 mM CaCl2 and the TRX-6His-S-tag was cleaved overnight at RT with enterokinase protease (NEB). The ET domain was further purified using Nickel-NTA resin (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... the myc tag was removed from the Ccz1-myc plasmid using NEB site-directed mutagenesis (SDM) kit (NEB) following manufacturer’s instructions and primers selected using NEBaseChanger tool (table 1) ...
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bioRxiv - Cell Biology 2022Quote: ... the HA tag and the Bub3 genomic fragment were inserted into pPEE37 (James Fraser) using Gibson assembly (NEB). Expression of HA-Bub3 was confirmed by immunoblotting with anti-HA antibodies ...
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bioRxiv - Synthetic Biology 2023Quote: ... HDR enhancing factors and MS2 tag were cloned between Esp3I sites by Golden Gate Assembly (New England Biolabs). All the primers are listed in Supplementary Table 2 ...
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bioRxiv - Plant Biology 2023Quote: ... AtPAXXL228E fused to hexa-histidine followed by a GST tag (pGAT3-atpaxxL228E) was expressed in BL21(DE3) (NEB) and purified using a 5 ml GSTrap HP column (Cytiva) ...
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bioRxiv - Biochemistry 2022Quote: ... a Thioredoxin-tag (Trx) and a TEV cleavage site via restriction digestion (NcoI/XhoI, New England BioLabs®) and subsequent ligation (T4 DNA Ligase ...
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bioRxiv - Biophysics 2023Quote: ... assembled with GST tag from pGEX6P plasmid and inserted into pACEBac1 vector via HIFI DNA assembly kit (NEB). The full length KIF5B in pACEBac1 was replaced with a C-terminal TEV-Twin-Strep-tag via HIFI DNA assembly kit (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... and in vitro bacterial cultures was used as template in reverse transcription reactions using the Murine Moloney Leukemia Virus Reverse Transcriptase (NEB M0253S) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... MWCO 10,000 filters and then digested with 10 µl Tobacco etch virus nuclear-inclusion-a endopeptidase (TEV) (New England Biolabs, Ipswich, MA) overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: Toxins were expressed with (His)6-tags at their C-termini in competent C43 (DE3) Escherichia coli cells (NEB) for HlgA and in BL21 (DE3 ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Microbiology 2020Quote: ... downstream of and in-frame with the 3X FLAG tag using the native restriction sites AgeI and NheI (NEB). Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... DGRC) downstream of and in-frame with the 3X FLAG tag using Gibson assembly (HiFi DNA assembly mix, NEB). Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich) ...
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bioRxiv - Biophysics 2020Quote: ... The point mutants were subsequently cloned in the pNZopuAHis plasmid (C-terminal 6-HIS-tag) using EcoRV-AlnwnI (NEB) for the substrate-binding domain mutants and βamHI-AlwnI (NEB ...
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bioRxiv - Cell Biology 2020Quote: Sec24D was subcloned from pEGFP Sec24D into pcDNA3.1 with an N-terminal myc-6xHis tag using Gibson Assembly (E5510, New England Biolabs). pcDNA3.1 was linearized with Not1 (R3189 ...
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bioRxiv - Biophysics 2020Quote: ... with TEV-cleavable 6x-His tag at N-terminal of the gene cloned between NheI (New England Biolabs Inc.) and HindIII (New England Biolabs Inc. ...
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bioRxiv - Microbiology 2021Quote: ... DGRC) downstream of and in-frame with the 3X FLAG tag using Gibson assembly (HiFi DNA assembly mix, NEB). Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... Two nested single-tail adapter/tag (STAT)-PCR [29] were performed with LongAmp Hot Start Taq polymerase (NEB, M0533S) using adaptor binding P5_1 and P5_2 primers and corresponding insert primer pairs (see Supplementary Table 2) ...
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bioRxiv - Biochemistry 2022Quote: ... The N-linked glycans and the CBP tag were cleaved by endoglycosidase H (Endo H, ∼0.2 mg per 10–15 mg purified protein, New England Biolabs) and HRV-3C protease (∼2 mg per 10–15 mg purified proteins ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were labelled with the cleavable SNAP-tag probe BG-S-S-549 (a gift from New England Biolabs) in complete medium for 30 min at room temperature ...
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bioRxiv - Cell Biology 2019Quote: The fluorescent SNAP-tag substrate BG-CF640R was synthesized by amidization reaction of BG-NH2 (New England Biolabs #S9148S) and CF640R-NHS ester (Biotium #92108) ...
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bioRxiv - Microbiology 2021Quote: ... downstream of and in-frame with the 3X FLAG tag using the native restriction sites AgeI and NheI (NEB). Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... a Hisx6 sequence tag was inserted into the BamH1 and Xho1 sites of MCS2 of pSNAPf (New England Biolabs) to create pSNAPf_Hisx6 ...
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bioRxiv - Molecular Biology 2020Quote: ... pET28a-6xHis-MBP-Tev cleavage site-mGFP-FUS-TIS-Strep-tag II was transformed into BL21 E.coli (New England Biolabs). Two fresh colonies were cultivated overnight in 2 × 50 ml SOB medium at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... a Hisx6 sequence tag was inserted into the BamH1 and Xho1 sites of MCS2 of pSNAPf (New England Biolabs) to create pSNAPf_Hisx6 ...
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bioRxiv - Molecular Biology 2022Quote: ... 404–561 with a C terminal His tag added was cloned into the pMAL-c5x vector (New England Biolabs), and were transformed in E ...
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... histidine tags were cleaved with LysC (200 ng/mg of protein; two hours at room temperature; New England Biolabs), and for all others ...
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bioRxiv - Biophysics 2023Quote: ... into a pSGEM construct with five consecutive repeats of the myc tag (Lörinczi et al., 2015) using NcoI and HindIII (New England Biolabs).
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bioRxiv - Biochemistry 2023Quote: ... the HA-epitope was replaced by a 6xHis tag using the Q5 Site Directed Mutagenesis Kit (New England Biolabs) to generate Ptch1 KR-His ...
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bioRxiv - Biophysics 2022Quote: ... The C-terminal Flag tag was introduced into pcDNA3.1-Spike-WT thanks to NEBuilder® HiFi DNA Assembly (NEB). The Flag tag was added during amplification of Spike-WT from pcDNA3.1-Spike-WT with the primers ...
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bioRxiv - Bioengineering 2023Quote: ... cells were incubated in dye solution (1 μM SNAP-tag ligand BG-AF647 (catalog no. S9136S, New England Biolabs), 1 mM DTT (catalog no ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated in dye solution (1 μM SNAP-tag ligand BG-AF647 (catalog no. S9136S, New England Biolabs), 1 mM dithiothreitol (catalog no ...
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bioRxiv - Cell Biology 2024Quote: ... MBP tags that co-eluted with NTF2L proteins were purified by incubating with amylose resin (New England BioLabs, #E8021L) overnight at 4°C and collecting the flow through the following day ...
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bioRxiv - Biochemistry 2020Quote: ... and G(5′)ppp(5′)A RNA (GpppA) (NEB, # S1406S) and G(5′)ppp(5′)G RNA (GpppG ...
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bioRxiv - Molecular Biology 2020Quote: ... A slurry of protein A or G magnetic beads (NEB) was used to capture enriched chromatin ...
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bioRxiv - Cell Biology 2020Quote: ... 75 µl of protein G magnetic bead suspension (S1430, NEB) pre-equilibrated in lysis buffer was added to each tube and incubation continued for additional 1 h at +4□C ...
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bioRxiv - Microbiology 2024Quote: ... 50 μL of protein G beads (New England Biolabs (NEB), S1430S ...
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bioRxiv - Microbiology 2024Quote: ... 50 μL of protein G beads (New England Biolabs (NEB), S1430S ...
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bioRxiv - Neuroscience 2021Quote: ... One μg of RNA was reverse transcribed in a 20 μl reaction using Moloney Murine Leukemia Virus Reverse Transcriptase (New England Biolabs, Evry, France) and random primers and cDNA obtained amplified using Q5® DNA Polymerase (New England Biolabs ...
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bioRxiv - Zoology 2023Quote: ... One μg of total RNA was reverse transcribed in a 20 μl reaction using Moloney murine leukemia virus reverse transcriptase (New England Biolabs, Evry, France) and random primers ...