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Citations for New England Biolabs :
251 - 300 of 585 citations for Recombinant Mouse Fcgr1 His & AVI Tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... was expressed as maltose-binding protein-tagged fusion protein using the pMAL(tm)c5X-vector (New England Biolabs, Ipswich, USA). The coding DNA sequence was amplified by PCR using gene-specific primers (for primer sequences ...
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bioRxiv - Developmental Biology 2021Quote: ... pFastBac dual vector containing the sequence for the N-terminally 6xHis-tagged human Naa15 and truncated human Naa10 (residues 1-160) sequences was digested using KpnI-HF (NEB) and XmaI (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... To visualize SNAP-tagged dCENP-A proteins cells were labelled with 3 µM SNAP-Cell TMR Star (New England Biolabs) for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... harboring the p6- GLuc / p6 genomes or pTM-ORF1 vectors and epitope-tagged inserts were restricted by the suitable restriction enzymes (Table 1) and subsequently ligated using the T4 DNA ligase (NEB). Finally ...
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bioRxiv - Genetics 2020Quote: ... and T3 (5’-AATTAACCCTCACTAAAGGG-3’) promoter-tagged PCR fragment from each gene using corresponding T7 and T3 RNA polymerase (T3:M0378S; T7:M0251S, BioLabs). Primers used for PCR are listed in Supplemental table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... To remove RNA secondary structure and anneal the mRNA capture primer 1 μL of tagged random hexamer (100 μM) and 0.5 μL of 10 mM dNTPs (dNTP solution set NEB - N0446S) were added to the sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids and siRNAs Bacterial expression constructs for His6-tagged p97 fragments were generated by Q5 Site-Directed mutagenesis (New England Biolabs) of pET15b-His6-p97 plasmid ...
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bioRxiv - Molecular Biology 2023Quote: Expression vectors encoding His6/SUMO-tagged SAHS proteins were obtained from Twist Bioscience and transformed into LEMO21(DE3) competent cells (New England Biolabs) according to the provider’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... myc-tagged AP3B1 immunoprecipitated and immobilised on Protein G-sepharose beads was incubated with CK2 (250 units; New England Biolabs) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... brucei BFs CN PIP5Pase exclusively expressing V5-tagged PIP5Pase WT or mutant D360A/N362A for 24 h using the magnetic mRNA isolation kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the SNAP-tagged fragment was assembled into a ApaI digested MBO2-HA plasmid using NEBuilder (New England Biolabs, Ipswich, MA). The final N-SNAP-MBO2-HA construct encodes an MBO2 polypeptide with a SNAP tag at its N-terminus and a 2-HA tag located between amino acids 885 and 886 of the original MBO2 sequence (Supplemental Figure S1).
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bioRxiv - Cancer Biology 2024Quote: ... Then the ORF of the ZNRF3 short variant replaced RNF43 and was assembled into the C-terminal FLAG-tagged vector using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and the GeneArt Gibson Assembly HiFi Master Mix (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3xFLAG- and HA-tagged ZNRF3 expression vectors were generated with the Q5 Hot Start High-Fidelity DNA Polymerase Kit (New England Biolabs) by replacing the FLAG-tag ...
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bioRxiv - Immunology 2021Quote: ... The recombinant plasmids (LentiCRISPRv2.gRNA10, LentiCRISPRv2.gRNA11) were then transformed into competent Escherichia coli (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins were expressed in Escherichia coli T7 Express lysY/Iq cells (New England Biolabs). For all proteins except PP1γ ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant plasmid was generated by a NEBuilder® HiFi DNA Assembly (New England Biolabs) reaction ...
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bioRxiv - Cell Biology 2020Quote: Recombinant MBP fusions to Nud1 or Nud17A were expressed from pMAL-c4X (New England Biolabs). Induction in Rosetta 2 cells (Novagen ...
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bioRxiv - Cancer Biology 2021Quote: Trastuzumab deglycosylation was achieved by adding 1.1 units of recombinant PNGaseF enzyme (New England BioLabs) per 1 μg of antibody ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant SARS-CoV-2 Omicron RBD was digested with EndoH over night (New England Biolabs). Recombinant hACE2 protein was digested using EndoH (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... Ribonucleoprotein (RNP) complexes were formed from sgRNA and recombinant Cas9 2NLS protein (New England Biolabs) mixed at a sgRNA to Cas9 ratio of 4.5 ...
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bioRxiv - Biochemistry 2022Quote: ... To complete the recombinant assembly reaction NEBuilder® HiFi DNA Assembly kit (New England Biolabs) was used with the combination of three different inserts produced by PCR ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction was initiated by addition of 10 U (pmol/min) of recombinant CK1 (NEB), CDK1-cyclin B1 (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reaction was started by adding 5000 units of recombinant Casein Kinase II (NEB, P6010S) and left to incubate for 20 min at 30°C with gentle agitation ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of the sgRNA was complexed with 20 pmol recombinant Cas9 endonuclease (NEB # M0646T) and injected into the C ...
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bioRxiv - Genomics 2021Quote: ... The PCR reaction consisted of 25 µL NEBNext Hi-Fidelity 2x PCR Master Mix (New England Biolabs NEB.M0541S), 7.5 µL H2O ...
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bioRxiv - Genomics 2021Quote: ... The PCR reaction consisted of 25 µL NEBNext Hi-Fidelity 2x PCR Master Mix (New England Biolabs NEB.M0541S), 7.5 µL H2O ...
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bioRxiv - Pathology 2020Quote: ... linearized and transcribed in vitro using the Hi-Scribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA). GAPDH IVT-RNA was purified by precipitation with lithium chloride followed by column purification using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... fully assembled complexes containing His-Rpn12 and MBP-Rpn6 were purified using a HisTrap and amylose resin (NEB), and cleaved with HRV-protease ...
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bioRxiv - Cell Biology 2020Quote: ... Igκ-LaNtα31-Myc-His was excised from pGEM®-5Zf(+)-LaNtα31 using NdeI and NsiI (New England Biolabs) and inserted into phK14-mCherry ...
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bioRxiv - Microbiology 2022Quote: ... Tagmented DNA was amplified by PCR using 1x NEBNext Hi-Fidelity PCR Master Mix (New England Biolabs, NEB), using Nextera Index i5 and i7 series PCR primers ...
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bioRxiv - Microbiology 2022Quote: ... Tagmented DNA was amplified by PCR using 1x NEBNext Hi-Fidelity PCR Master Mix (New England Biolabs, NEB), using Nextera Index i5 and i7 series PCR primers ...
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bioRxiv - Neuroscience 2023Quote: Codon-optimized human TDP-43-His LIC-A constructs were transformed into T7 express (New England Biolabs, #C3029J) E ...
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bioRxiv - Bioengineering 2023Quote: ... GLuc was inserted into the digested backbone through Gibson Assembly Hi-Fi kit (New England Biolabs, Ipswich, MA). AAV-hSyn-hM3Dq-RAM-d2tTA was constructed as previously described19 ...
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bioRxiv - Microbiology 2023Quote: ... Purified PCR products were incubated together in a thermocycler with 1X Hi-Fi DNA Assembly master mix (NEB) at 50°C for one hour ...
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bioRxiv - Biophysics 2021Quote: ... was filled in with a mixture of natural and biotinylated nucleotides by the exonuclease-deficient DNA polymerase I Klenow fragment (New England BioLabs). Reaction was conducted by incubating 10 nM λ-DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Ribodepleted RNA were then ligated to 10 pmol of a biotinylated 3’ adapter, (3’-Adap TAIL-seq, Supplementary Table 7) using 10 units of T4 RNA ligase 1 (NEB) in a final volume of 10 μl for one h at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... the 12-base 5’ overhang on each end was filled in with a mixture of unmodified and biotinylated nucleotides by the exonuclease-deficient DNA polymerase I Klenow fragment (New England BioLabs). The reaction was conducted by incubating 10 nM λARS1 DNA ...
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bioRxiv - Biochemistry 2022Quote: SNAP–tagged kinesin-8B-MD proteins (20 μM) were biotinylated in 50 μl reaction volumes by incubating with 40 μM SNAP-biotin (NEB) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... Biotinylated blunt ends were then ligated using a ligation reaction (663μl water, 120μl 10X NEB T4 DNA ligase buffer (NEB), 100μl 10% Triton X-100 (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... Circularised product is subjected to two rounds of rRNA depletion using biotinylated oligos [17] and streptavidin-coated magnetic beads (NEB) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: SNAPf–tagged Kinesin-8X-MD proteins (20 μM) were biotinylated in 50 μl reaction volumes by incubating with 40 μM SNAP-biotin (NEB) at 4 °C for 1.5 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... 5 μM of phosphorylated template oligo IF239 and 4.5 μM biotinylated primer oligo IF238 were annealed in T4 ligase reaction buffer (NEB B0202S). The mixture was heated to 75 °C for 5 min and cooled to 4 °C at a rate of −1 °C min−1 ...
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bioRxiv - Immunology 2021Quote: Antisense DNA probes (Table S7) were synthesized at Metabion AG and 3’ mono-biotinylated using terminal transferse (New England Biolabs) and Biotin-11-ddUTP (Jena Bioscience ...
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bioRxiv - Genomics 2021Quote: ... Array DNA used in directed methylation experiments was then biotinylated by filling in EcoRI and XbaI 5’overhangs with dGTP (NEB), α-thio-dCTP (Chemcyte) ...
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bioRxiv - Biophysics 2020Quote: ... was functionalized at one end by annealing with a biotinylated oligonucleotide/5Phos/AGGTCGCCGCCCTTTTT/Bio (IDT) followed by ligation with T4 DNA ligase (NEB). The DNA was purified using a bead purification kit (Qiaex II ...
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bioRxiv - Molecular Biology 2023Quote: Randomly biotinylated DNA templates for TECprobe-ML experiments were PCR amplified from a 5’ biotinylated linear DNA template using Vent (exo-) DNA polymerase (New England Biolabs) and primers HP4_5bio.R and PRA1_NoMod.F (for ZTP and fluoride templates ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated RNA was fragmented to approximately 200 – 500 bases with the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs E6150S) and precipitated by adding 1/10 volume of 3M sodium acetate pH 5.5 (ThermoFisher AM9740 ...
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bioRxiv - Biophysics 2023Quote: ... was filled in with a mixture of natural and biotinylated nucleotides by the exonuclease-deficient DNA polymerase I Klenow fragment (New England BioLabs). The reaction was performed by incubating 17 μg λ DNA ...
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bioRxiv - Biochemistry 2023Quote: ... 250 ng of Cy5-labeled and biotinylated DNA was incubated with 1 μg of streptavidin (SA, New England Biolabs, #N7021) in 25 μL of binding buffer (10 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA was enriched from crude cell lysate in two rounds using biotinylated oligo-dT (IDT Europe) and streptavidin beads (New England Biolabs). RNA isolations were performed in batches in a randomized order ...