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Citations for New England Biolabs :
501 - 550 of 993 citations for Recombinant Monkey CCL19 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... MnCl2 to a concentration of 1 mM and NEBuffer for Protein MetalloPhosphatases (NEB) diluted to a 1X concentration were added to both tubes ...
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bioRxiv - Biochemistry 2020Quote: Three microgram of full-length S protein were treated with PNGase F (NEB) for N-glycan release according to the manufacture’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... The protein extracts were incubated with alkaline phosphatase (Anthartic phosphatase, New England Biolabs) in EBX reaction buffer (50mM HEPES/KOH pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF) and proteins were purified by amylose beads (New England Biolabs) and eluted in wash buffer (20mM Tris pH:7.6 ...
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bioRxiv - Microbiology 2021Quote: ... Nitrocellulose membrane-transferred proteins were incubated with anti-PfGet3 or anti-MBP (NEB), probed with corresponding HRP-conjugated secondary antibodies and developed by chemiluminescence.
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bioRxiv - Microbiology 2020Quote: The final microinjection mix contained 322 ng/μL spCas9 protein (New England Biolabs) with 40 ng/μL of each sgRNA and 127 ng/μL of the ssDNA repair template ...
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bioRxiv - Biophysics 2021Quote: ... pH 8.4 using cAMP-dependent protein kinase A (New England Biolabs, Frankfurt, Germany). The phosphorylated vimentin was mixed at the desired ratios of 1 ...
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bioRxiv - Cell Biology 2020Quote: All in vitro kinase assays were done in NEBuffer for protein kinase (NEB) at 30°C ...
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bioRxiv - Biochemistry 2022Quote: ... Purified Vg was dephosphorylated with Lambda Protein Phosphatase (New England BioLabs, MA, USA). The samples were incubated with 1 µL Lambda Protein Phosphatase ...
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bioRxiv - Cell Biology 2021Quote: ... solubilized proteins were treated with Endo H and PNGase F (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... and PODXL2 were mixed with Protein Deglycosylation Mix II (New England Biolabs, #P6044S). The suggested protocol by the supplier was used with the exception of incubation time (the proteins were only incubated at RT for 20 h).
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bioRxiv - Plant Biology 2020Quote: ... Immunoprecipitation was performed using protein A magnetic beads (New England BioLabs, Ipswich, MA). Following the Proteinase K treatment of each sample ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with 10 U/ul Lambda Protein Phosphatase(New England Biolabs, #P0753) at 30°C for 30 minutes ...
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bioRxiv - Cancer Biology 2019Quote: Purified hTERT_191-306 proteins were incubated with CDK1-cyclin B (New England Biolabs) or purified IKK2_2-664 proteins ...
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bioRxiv - Microbiology 2020Quote: ... Molecular weights were determined using protein standards (New England Biolabs P7712, or P7719). Puromycin incorporation assays were performed essentially as described in (38) ...
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bioRxiv - Cell Biology 2021Quote: ... and biotin-pupE labelled proteins were enriched by streptavidin magnetic beads (NEB, S1420S) and identified by mass spectrometry analysis (Liu et al. ...
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bioRxiv - Biochemistry 2020Quote: All proteins were expressed in Escherichia coli NiCo21 (DE3) cells (New England BioLabs). To co-express multiple proteins ...
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bioRxiv - Immunology 2022Quote: ... ∼50 μg of protein were incubated with 20 units of λPP (NEB, USA) for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... following the final wash proteins were eluted in Glycoprotein Denaturing Buffer (NEB, P0702S) for 10 mins at 100°C and then resuspended in EndoH (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 4.2 ng/μl extreme thermostable single-stranded DNA binding protein (New England Biolabs) and 0.02 U/μl SuperFi DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... cross-linked virion samples were deglycosylated using Protein Deglycosylation Mix II (P6044, NEB) under denaturing conditions according to manufacturer’s instructions ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The MBP tagged proteins were purified by using Amylose Resin (New England Biolabs) and Ultrafiltration tubes (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... the REC114-MEI4 protein-containing supernatants were loaded onto an Amylose resin (NEB). After extensive washing with a buffer containing 20mM Tris pH 8 ...
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bioRxiv - Developmental Biology 2023Quote: ... The two gRNAs were injected together with Cas9-NLS protein (New England Biolabs) into one-cell stage embryos ...
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bioRxiv - Plant Biology 2023Quote: ... MBP-tagged and GST-tagged proteins were detected using anti-MBP (E8032S, NEB) and anti-GST antibody (60-021 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by the addition of 1uL TEV Protease (New England Biolabs) and incubated for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein A magnetic beads (Cat#S1425S, New England Biolabs Inc, Ipswich, MA, USA) were aliquoted (20 □l ...
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bioRxiv - Developmental Biology 2023Quote: ... 2ng gRNA was injected together with 1.9nM Cas9 protein (New England Biolabs M0386T) and 10% Phenol Red (Sigma- Aldrich P0290 ...
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bioRxiv - Cancer Biology 2022Quote: ... DHFR control plasmid from PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used as backbone for subsequent ligations ...
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bioRxiv - Developmental Biology 2023Quote: ... Freshly laid wild-type TU-strain embryos were injected with SpCas9 protein (NEB) mixed with gRNAs (~300 ng/ul) ...
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bioRxiv - Biochemistry 2023Quote: ... and (MBP)2-HSPC300 proteins were purified through amylose beads (New England Biolabs). The Sra1/Nap1 dimer was obtained by co-expressing His6-Sra1 and untagged Nap1 in Tni insect cells (Expression Systems) ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was pre-cleared using protein G magnetic beads (New England Biolabs). The cleared supernatant was then incubated with 3 µg anti-myc antibody (SIGMA M4439 monoclonal anti c-myc or Abcam ab206486 rat mAb to myc tag (9E10) ...
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bioRxiv - Plant Biology 2023Quote: ... The MBP-fused protein was purified with Amylose Resin (E8021V; New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... either 1.8 mg USP2 or 800 U Lambda protein phosphatase (New England BioLabs) was added as indicated ...
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bioRxiv - Cell Biology 2024Quote: Proteins were expressed in Escherichia coli T7 Express lysY/Iqcells (New England Biolabs) in 2×TY medium at 37°C to an OD600 of 0.8–1.2 before inducing protein expression by addition of 0.4 mM isopropyl β-D-thiogalactopyranoside (IPTG) ...
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bioRxiv - Microbiology 2024Quote: ... His- tagged proteins were purified by NEBExpress® Ni-NTA Magnetic Beads (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... the protein-bound RNA was dephosphorylated with Quick CIP (M0525S, New England Biolabs) for 10 min in 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted proteins were loaded onto a 6 mL amylose column (New England Biolabs), washed in 50 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Synthetic Biology 2024Quote: All protein expression constructs (Supplementary Table S1) were cloned using Gibson assembly (NEB, where double-stranded DNA fragments are either ordered from IDT as gBlocks or PCR amplified from existing constructs with at least 25-bp overlap in sequence ...
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bioRxiv - Biochemistry 2024Quote: ... the protein bound RNA was dephosphorylated with Quick CIP (M0525S, New England Biolabs) for 10 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... dCas9-SNAP protein samples were incubated with SNAP-Surface Alexa Fluor 647 (NEB S9136) at 37°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... The proteins were expressed in BL21 DE3 competent cells (New England Biolabs, Ipswich, MA). Protein purification was undertaken using nickel-affinity chromatography (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutant protein expression vectors were generated using the Q5 Mutagenesis Kit (New England BioLabs) and proteins overexpressed and purified the same as wild-type.
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bioRxiv - Cancer Biology 2021Quote: ... Precipitated proteins were resuspended in 150 μL of Glycoprotein Denaturing Buffer (New England Biolabs) and incubated at 100 °C for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... reduced and alkylated and proteins were digested with first PNGase F (New England BioLabs), endoproteinase Lys-C (Promega ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... against a Colour Prestained Protein Standard (Broad Range 10-250 kDa, New England BioLabs) and resolved by electrophoresis for 70 minutes at 150-180V ...
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bioRxiv - Neuroscience 2020Quote: ... AP fusion proteins are quantified using calf intestinal alkaline phosphatase (CIP, New England Biolabs) activity ...
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bioRxiv - Biochemistry 2020Quote: ... His-SNAP-EB3 proteins were then coupled to SNAP-Cell 647-SiR dye (NEB) by incubation at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and then resuspended in phosphatase buffer with 200U lambda protein phosphatase (New England Biolabs) at 30 °C for 20 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein sample was concentrated by ultrafiltration and incubated with PNGaseF (New England Biolabs) overnight.