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Citations for New England Biolabs :
251 - 300 of 1129 citations for Recombinant Influenza A virus HA protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... a DNA fragment containing TDP-43 was inserted into pCold I-GstTev-HA by NEBuilder HiFi DNA Assembly Master Mix (NEB).
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bioRxiv - Molecular Biology 2019Quote: ... followed by the insertion of a DNA fragment containing C-terminal 2×HA tag by NEBuilder HiFi DNA Assembly Master Mix (NEB). Finally ...
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bioRxiv - Cell Biology 2019Quote: ... the eGFP-FLAG-HA-MLKS2 sequence was PCR amplified with att flanking primers (Table S2) using high fidelity Q5 polymerase (NEB) and cloned into pDONR221 vector by BP cloning (Cat ...
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bioRxiv - Molecular Biology 2019Quote: ... that were integrated into various backbone plasmids (e.g. p28-GFP, p28-HA, or TCV_sg2R) using Gibson Assembly cloning (NEB, Ipswich, MA). The identities of all new constructs were verified with Sanger sequencing.
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bioRxiv - Microbiology 2019Quote: Site-directed mutagenesis of low copy number vector p3187 harbouring siiA::HA under control of PsiiA was performed by using the Q5 SDM kit according to the manufacturer instructions (NEB) (Table S9) ...
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bioRxiv - Cancer Biology 2020Quote: The Flag-HA-USP18WT construct was purchased from Addgene (#22572) and the Flag-HA-USP18C64R/C65R construct was generated using the Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) and the primers OL87 ...
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bioRxiv - Evolutionary Biology 2020Quote: Deep mutational scanning libraries were generated using degenerate primers to amplify TRIM5α-HA in pQCXIP using high-fidelity Q5 polymerase (NEB). Degenerate primers contained a single NNS codon (N = A/T/C/G ...
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bioRxiv - Biochemistry 2022Quote: ... The library was cloned into a pENTR1A plasmid backbone with a C-terminal YFP HA reporter tag using Gibson Assembly (HiFi DNA Assembly Cloning Kit, New England Biolabs, (NEB), Ipswich ...
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bioRxiv - Microbiology 2022Quote: ... and subsequently modifying the plasmid to add the HA-tag coding sequence using the Q5 Site-Directed Mutagenesis kit (NEB) or the mEos3.2 coding sequence using InFusion (Clontech) ...
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bioRxiv - Microbiology 2022Quote: ... NNK-mutagenized HA amplicons were then cloned into pDZ vector by T4 ligation (T4 DNA Ligase, New England BioLabs Inc.). The ligation product was then used to transform DH10B competent cells (MegaX DH10B T1 ...
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bioRxiv - Cell Biology 2022Quote: ... pIREShyg2-HA-hAdipoR2-cMYC(H348A) construct was generated using PCR-based mutagenesis (Q5-site-directed mutagenesis kit, New England Biolabs) with the following primers ...
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bioRxiv - Cell Biology 2022Quote: ... was fused to an HA-tag at its N-terminus during PCR and cloned into the lentiviral vector pLJM1 linearized with AgeI (NEB) and EcoRI (NEB ...
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bioRxiv - Neuroscience 2019Quote: ... encoding full length Ppp3cc was amplified with primers 5’- AGATTACGCTATCTGTACAGAATTCACCATGTCCGTGAGGCGC-3’ and 5’-GGCCGCTAGCCCGGGTACCGAATTCTTACAGGGCTTTCTTTCCATGGTC-3’ and inserted into pCAG-HA vector using NEB Builder (NEB).
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bioRxiv - Molecular Biology 2020Quote: ... This plasmid was digested with XbaI and NheI enzymes to release TcZC3H12-HA sequence followed by the Neomycin expression cassette and treated with One Taq DNA polymerase (NEB) to allow for cloning in pCR2.1-TOPO plasmid (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: The annealed sgRNA oligos were diluted 1:200 in water and ligated into the pX459 plasmid that has been linearized with restriction enzyme BbsI (New England Biolabs). The ligation mixture contained 2 ul of diluted sgRNA oligos ...
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bioRxiv - Microbiology 2021Quote: ... The fragment of sNTurboID was PCR amplified from pLX304 CMV FKBP-V5-sTurboID (N) and cloned into the pcDNA5-VP35-HA using NEBuilder HiFi DNA Assembly Master Mix (NEB). The fragment of VP35-sNTurboID ...
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bioRxiv - Developmental Biology 2021Quote: ... each CBE4max-SpG and CBE4max-SpRY sequence has been inserted into pCS2+ plasmid linearized with EcoRI using the Gibson Assembly Cloning Kit (New England Biolabs). The fragment has been amplified using the primers F-5’-TGCAGGATCCCATCGATTCGGCCACCATGAAACGGACAG -3’ and R-5’-TAGAGGCTCGAGAGGCCTTGTCAGACTTTCCTCTTCTTCTTGG -3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... added to 200 μl of 2.5% (v/v) of MagStrep “type3” XT beads (IBA Lifescience) that has been blocked overnight with 1 mg/ml BSA (NEB) and 0.5 mg/ml tRNA (SigmaAlrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and rps10 (ENSDARG00000034897) were amplified by RT- PCR and cloned into pCS2+HA using NEBuilder HiFi DNA Assembly (New England BioLabs). The pCS2+HA was generated by inserting synthetic DNA fragments with HA- tag sequence into pCS2+ via EcoRI/XbaI restriction sites ...
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bioRxiv - Microbiology 2023Quote: ... PYD and HD mutants were generated from full length IFI207-HA plasmids and the R1-PYD mutant from R1-IFI207 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). IFI207-HIN plasmids were generated by PCR amplification of the HIN domain from full length IFI207-V5 expression plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... with s 3×HA sequence fused to the C-terminus and the ADH1 terminator sequence was amplified from genomic DNA of a yeast strain expressing Opi1-HA and subsequently cloned into the pRS416 vector using XhoI and SacII restriction enzymes (New England Biolabs). To overexpress INO1 and KCS1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... while the pML104-PDR1 vector has a guide sequence 5’- CTGGATAAACGTCGCTCCAC-3’ introduced by Q5 polymerase PCR (New England Biolabs) and In-Fusion Snap Assembly (Takara ...
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bioRxiv - Molecular Biology 2023Quote: ... were amplified out of cDNA from Arabidopsis Col-0 or an SICp:SICm-Venus-HA transgenic line (see below) by PCR with Q5 High Fidelity Polymerase (New England Biolabs, www.neb.com). Primers “WARP2cdsGATEF” and “WARP2cdsR” were for SIC and primers “DBR1_CDS_nostp_CACC_F” and “DBR1_CDS_stp_R” were for DBR1 (Table S1) ...
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bioRxiv - Biochemistry 2023Quote: Human HA-DHFR was subcloned from human cDNA to pcDNA4/TO by PCR using Q5 High Fidelity 2X mastermix (NEB). Human FPGS-FLAG and GGH-FLAG were subcloned from cDNA clones MHS6278-202755815 (Horizon Discovery ...
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bioRxiv - Genomics 2023Quote: ... Library preparation has been done with 250ng of input RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) followed by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Cell Biology 2023Quote: ... the SNAP-tagged fragment was assembled into a ApaI digested MBO2-HA plasmid using NEBuilder (New England Biolabs, Ipswich, MA). The final N-SNAP-MBO2-HA construct encodes an MBO2 polypeptide with a SNAP tag at its N-terminus and a 2-HA tag located between amino acids 885 and 886 of the original MBO2 sequence (Supplemental Figure S1).
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bioRxiv - Cell Biology 2023Quote: Mutations in RAD54L were generated in pENTR1A-RAD54L-HA [30] using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) (Table S5) ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3xFLAG- and HA-tagged ZNRF3 expression vectors were generated with the Q5 Hot Start High-Fidelity DNA Polymerase Kit (New England Biolabs) by replacing the FLAG-tag ...
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bioRxiv - Microbiology 2021Quote: ... were used to amplify the pQE60- ompA recombinant plasmid (Size- 4.5 kb) using Phusion high fidelity DNA polymerase (NEB). The reaction mixture was heated at 950C for 10 minutes for the plasmid’s denaturation ...
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bioRxiv - Microbiology 2021Quote: ... 40 μL purified supernatant were combined with 2,500 units recombinant glycerol-free PNGase F and Glycobuffer 2 (New England Biolabs) following the manufacturer’s protocol for non-denaturing digestion and incubated at 37°C for 5 hours ...
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bioRxiv - Genetics 2022Quote: ... Eggs were then injected under air-dry conditions with a solution containing 300ng/μl of recombinant Cas9 Nuclease (NEB) and 100ng/ul each of four sgRNAs targeting the first exon of the Oatp74D gene (S2 Table) ...
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bioRxiv - Microbiology 2023Quote: ... Enzymatic deglycosylation was performed on denatured PrPres with 1,000 U of recombinant PNGase (peptide N-glycosidase F; New England Biolabs) for 2h at 37°C in 1% Nonidet P40 and the manufacturer’s buffer.
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bioRxiv - Microbiology 2022Quote: ... Recombinant plasmids encoding a catalytically inactive 3Dpol (3Dneg) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S). Nucleotides at position 6891 and 6892 of the CVB3/28 genome were mutated from AT to GC ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were pelleted at 500 x g for 5 minutes and resuspended in 8µg/mL recombinant albumin (New England Biolabs) in dPBS-/- ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at room temperature before being incubated with 1.0 µg/mL of recombinant human histone H3.1 (New England Biolabs, #M2503S) for 1 h at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... The immunoprecipitated protein was incubated with λ protein phosphatase using manufacturer’s protocol (Cat. # P0753S, NEB). The treated samples were resolved on SDS-PAGE and western blotting performed using OsFD7 antibody.
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bioRxiv - Plant Biology 2022Quote: ... The protein extracts were incubated with Lambda Protein Phosphatase (λ-PPase) (NEB, Cat. No. P0753S) at 30 □ for 30 min and subsequently added 5×SDS sample buffer incubated at 95 □ for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... PfCPT-HA-TetR-DOZI and PfCPT-TetR-DOZI products were linearized for transfection using HPaI (New England BioLabs, Ipswich, MA, USA). Primers forward P15 and reverse 16 were used for annealing and insertion of the guide RNA into pUF1 vector previously digested.
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bioRxiv - Microbiology 2019Quote: ... and 1 ml of lysate (corresponding to 50 ml of the original culture) was transferred into tubes containing 50 μl of pre-washed (3x) magnetic α-HA beads (NEB) and incubated for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated as follows: HA-tagged and untagged ORFs with pHCMV homologous overhanging sequence were either PCR amplified using Q5 polymerase (NEB, M0491S) or synthesized (IDT ...
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bioRxiv - Biochemistry 2022Quote: ... The library was cloned into a pENTR1A plasmid backbone with a C-terminal YFP HA reporter tag using Gibson Assembly (HiFi DNA Assembly Cloning Kit, New England Biolabs, (NEB), Ipswich ...
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bioRxiv - Genomics 2021Quote: The HA-AID-CTCFcDNA-AID-eGFP-blasticidin vector was assembled by Gibson Assembly (NEBuilder HiFi DNA Assembly Master Mix, NEB, E2621L) in the pENTR221 kanamycin vector using the following templates ...
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bioRxiv - Biochemistry 2020Quote: ... (DU67916) was made by removing the HA and replacing with Twinstrep 3C using Q5 site-directed mutagenesis (New England Biolabs E0552S) in pcDNA5D FRT/TO HA SARS-CoV-2 Nsp3 743-1072 (PL-pro ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the pML104-GAL1 vector has a guide sequence 5’- CTCTTAAATTATAGTTGGTT-3’ introduced by Q5® Site-Directed Mutagenesis Kit (New England Biolabs) (Hu et al. ...
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bioRxiv - Cell Biology 2023Quote: ... APEX2-ATP7B-EGFP and APEX2-mKO2-HA-ATP7A constructs were made on the existing plasmid using NEBuilder HiFi DNA Assembly (NEB #E2621). Mutations on eGFP-ATP7B were prepared following Q5 Site-Directed Mutagenesis Kit (NEB #E0554 ...
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bioRxiv - Molecular Biology 2023Quote: ... the pUbi63E-KDM5-HA plasmid was generated by replacing the Flag-Ubiquitin CDS sequence with the KDM5-HA CDS sequence using Gibson assembly technology provided by NEB (# E5510S).
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bioRxiv - Synthetic Biology 2023Quote: ... reaction was performed to remove sfGFP and replace it with the cassette components (P1-FLAG, P2-Substrate, P3-HA, P4-ERS). This plasmid was transformed into competent Escherichia coli (E. coli) (NEB#C2984H), purified (QIAGEN #27106 ...
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bioRxiv - Neuroscience 2023Quote: ... Integration of the amplified mouse genomic DNA into the donor plasmid and integration of the 3x HA tag before the stop codon were each carried out by Gibson assembly (NEB, USA). HDR into C57BL/6J background mouse embryos was carried out by mixing the plasmid donor ...
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bioRxiv - Microbiology 2024Quote: The pLIC-HA-DHFR-TS (gift from Jeroen Saeij, University of California, Davis) plasmid was treated simultaneously with PacI (NEB #R0547S) and AvrII (NEB #R0174S ...
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bioRxiv - Microbiology 2020Quote: Template DNA was amplified by PCR using custom DNA primers (Table S3) and recombinant Phusion Hot Start polymerase (New England Biolabs). In vitro transcription was carried out in a volume of 2.5 mL comprising 1.0 mL of PCR reaction as template ...