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Citations for New England Biolabs :
201 - 250 of 739 citations for Recombinant Human S100A3 His & MBP tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Proteins were separated by SDS-PAGE and detected by immunoblotting using anti-MBP (New England BioLabs, E8032S), anti-GST (MBL ...
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bioRxiv - Plant Biology 2020Quote: ... The NRPD1-Maltose Binding Protein (MBP) fusion protein was affinity purified using Amylose resin (New England Biolabs) and eluted with 20 mM maltose ...
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bioRxiv - Plant Biology 2022Quote: ... The soluble fraction of MBP-MP was applied to pre-equilibrated amylose agarose resin (New England Biolabs). After washing the column three times with amylose column buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Bound protein was detected by western blot using mouse anti-MBP diluted 1:10,000 (New England Biolabs), fluorescent secondary antibodies (Li-Cor Biosciences) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cascade complexes were purified via the N-terminal maltose binding protein (MBP) tag using amylose beads (NEB) and eluted with lysis buffer containing 10 mM maltose ...
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bioRxiv - Biochemistry 2023Quote: ... MBP pulldown assays were performed similar to GST pull down assays using Amylose resin (New England Biolabs) and 1 nmol of MBP-Oskar 292-325 and 5 nmol of Smaug 37-281.
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bioRxiv - Molecular Biology 2023Quote: MBP-Nla6 DBD was purified according to instructions in the pMALTM Protein Fusion & Purification System manual (NEB). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... solutions containing polySH3 and cleaved MBP- and His6-tags were passed over Amylose resin (New England Biolabs) to remove excess MBP ...
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bioRxiv - Molecular Biology 2021Quote: ... SET8 recombinant enzyme (New England Biolabs # M0428S) was incubated with recombinant active PARP1 (Trevigen # 4668-100-01 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3µl recombinant AsiSI endonuclease (10U/µL, NEB) was added to three biological replicates and incubated (2 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... and recombinant shrimp alkaline phosphatase (rSAP, NEB) overnight and PCR purified (Qiagen QIAQuick PCR Purification Kit ...
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bioRxiv - Synthetic Biology 2023Quote: ... and recombinant shrimp alkaline phosphatase (all NEB) at 37°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... 0.2 mg/mL recombinant albumin (NEB B9200S), 1 μM RT primer ...
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bioRxiv - Plant Biology 2023Quote: ... SNAP-tagged proteins were labelled with Cell-SiR647 (NEB, Frankfurt, Germany). Labeling was done at RT for 1hr ...
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bioRxiv - Systems Biology 2024Quote: Each tagged-ORF plasmid subpool was treated with I-SceI (NEB) in the provided buffer (at a volume of 50uL for ∼1ug hORF plasmids and 200uL for ∼4ug vORF plasmids) ...
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bioRxiv - Biochemistry 2019Quote: ... which followed digestion of affinity tag and deglycosilation by TEV protease and MBP-fusion endoglycosidase (New England Biolabs), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... The cleaved 6xHis-MBP tag was removed by incubating the solution with Ni-NTA magnetic beads (NEB; S1423) for 1 hour at 4 °C ...
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bioRxiv - Biochemistry 2019Quote: ... transferred at 300 mA for 90 minutes and probed overnight with α-MBP (1:10000, New England BioLabs) or α-ORC (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (1:10.000, used with 1:10.000 secondary horseradish peroxidase-conjugated anti-mouse antibody; NEB).
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bioRxiv - Microbiology 2021Quote: ... A small fraction of the refolded MBP-ACE2NTD fusion protein was further purified by amylose magnetic beads (NEB) according to the protocol provided by the manufacturer.
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bioRxiv - Plant Biology 2022Quote: ... and the ubiquitinated ERECTA_CD were detected by IB analysis with anti-MBP (E8032, 1:10,000, New England Biolabs) as primary antibody ...
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bioRxiv - Plant Biology 2024Quote: ... The beads were then boiled in SDS-loading buffer for subsequent western blotting using MBP antibodies (NEB, E8032S). Histone peptides utilized in this study were synthesized by EpiCypher and included H3 (1–32 aa) ...
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bioRxiv - Microbiology 2019Quote: ... or Bam HI and Xho I (New England BioLabs, MA). The column isolated DNA fragments were ligated into our modified pFastBac1 plasmid having 6xHis tag at C-terminus ...
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bioRxiv - Molecular Biology 2020Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... Followed by subcloning the PCR amplicon in Bam-HI (NEB) restriction digested pCW57-tGFP-2A-MCS plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... using NEBuilder Hi-Fi Assembly (New England Biolabs, Cat. #E2621). The pMB1052 construct was subsequently cut using BspDI and EcoRV (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... The Hi-Scribe T7 High Yield RNA synthesis kit (NEB) was used to generate RNA transcripts at 37°C for 16 hours ...
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bioRxiv - Systems Biology 2024Quote: ... using the NEBuilder Hi-Fi Assembly kit (New England Biolabs). To ensure representation of all variants in the population ...
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bioRxiv - Cancer Biology 2023Quote: ... cut with hi-fidelity EcoRI and BamHI (New England Biolabs), and gel purified ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified on glutathione-Sepharose beads (New England Biolabs), and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Genomics 2022Quote: ... and 30 nM recombinant Cas9 nuclease (NEB, M0386S) at 37°C for 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... 1 μg of recombinant H4 (New England Biolabs) and 100 μM S-adenosyl methionine (SAM ...
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bioRxiv - Cell Biology 2023Quote: ... 100 or 500 units of recombinant CK2 (NEB), 5µM or 15µM silmitaserib (CK2 inhibitor ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µL 1U recombinant shrimp alkaline phosphatase (NEB) and 2.5 µL 10x CutSmart buffer (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/ml recombinant albumin (New England Biolabs), and 0.1 mg/ml RNase A (QIAGEN)] ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/ml recombinant albumin (New England Biolabs), 0.1 mg/ml RNase A (QIAGEN) ...
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bioRxiv - Cancer Biology 2020Quote: ... The adaptor-ligated DNA was hybridized to custom Agilent biotinylated oligonucleotide probes across a 700bp region (53032 probes; 4.684 Mbp oligo) and then pulled-down by Dynabeads M-270 Streptavidin beads (NEB). The post-capture DNA library was amplified with STARR_in-fusion_F and STARR_in-fusion_R primers (Supplementary Table 1) ...
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bioRxiv - Biophysics 2019Quote: ... 115 amino acids) fused to the C-terminus of maltose binding protein (MBP) in pMALX vector (New England Biolabs), MBP-5-HT3A-ICD-pMALX ...
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bioRxiv - Molecular Biology 2020Quote: ... pET28a-6xHis-MBP-Tev cleavage site-mGFP-FUS-TIS-Strep-tag II was transformed into BL21 E.coli (New England Biolabs). Two fresh colonies were cultivated overnight in 2 × 50 ml SOB medium at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... Cleavage of GS linkers from MBP was done in column using 1 unit of TEV protease (Catalog P8112S, NEB) for every 2 µg of fusion protein in 1X TEV buffer (Catalog P8112S ...
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bioRxiv - Plant Biology 2023Quote: ... The precipitates were eluted into 30μL 1×SDS loading buffer and detected using anti-Myc and anti-MBP antibody (1:5,000, New England Biolabs).
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bioRxiv - Cell Biology 2024Quote: ... MBP tags that co-eluted with NTF2L proteins were purified by incubating with amylose resin (New England BioLabs, #E8021L) overnight at 4°C and collecting the flow through the following day ...
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bioRxiv - Genetics 2024Quote: DNA from the VIU and MBP breeding program samples was extracted using the Monarch Genomic DNA Purification kit (NEB) using the enzymatic cleanup tissue extraction protocol with proteinase K and RNase A and quantified using a BioSpectrometer (Eppendorf) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The two fragments were assembled using HI-FI assembly (NEB E5520S). To construct the Prcan-1-R1∷mCherry and Prcan-1-R2∷mCherry plasmids ...
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bioRxiv - Immunology 2021Quote: ... The fragments were assembled using Hi-Fi DNA Assembly Mix (NEB). For the DonorgRNA vector ...
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bioRxiv - Genomics 2021Quote: ... and 25 µl 2x NEBNext Hi-Fi PCR mix (NEB, USA) per reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10 μg/mL) and apyrase (25 mU/mL, NEB); the suspension was incubated for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nb35–His (10 µg/ml) and apyrase (25 mU/ml, NEB). The suspension was incubated for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... For all Gibson Assemblies the NEB Hi-Fi Assembly Mastermix (NEB) was used ...