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Citations for New England Biolabs :
201 - 250 of 1401 citations for Recombinant Human CD40 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Dephosphorylated RNA was then 5’ end-labeled with 20U T4 PNK (NEB) and 30μCi [g32-P]ATP (Perkin-Elmer) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were labeled with 500nM SNAP-Surface 649 (New England Biolabs, #S9159S) for 5 minutes at 37°C for TIR-FM imaging or 1μM permeable SNAP-Cell 647-SiR (New England Biolabs ...
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bioRxiv - Immunology 2020Quote: ... The enriched Br-dU labeled RNAs were incubated with RppH (NEB, #M0356S) and with T4 PNK (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... Probes were labeled with ATP-P32 using T4 polynucleotide kinase (NEB®) and blot was exposed to a phosphor screen (GE® ...
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bioRxiv - Immunology 2020Quote: ... The enriched Br-dU labeled RNAs were incubated with RppH (NEB, #M0356S) and with T4 PNK (NEB ...
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bioRxiv - Genomics 2023Quote: ... Probes were labeled with 32P by nick translation (New England Biolabs, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... dinucleotide primers were 5’-labeled using T4 polynucleotide kinase (New England Biolabs) and added at 20 µM without pre-annealing to the template ...
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bioRxiv - Cell Biology 2024Quote: ... Transfected cells were labeled with 1 µM SNAP-Surface 674 dye (NEB) for 30min followed by washing two times for 10 min with 300 µl MES300 per well ...
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bioRxiv - Systems Biology 2024Quote: ... human (E6320, New England Biolabs, USA) and sequenced on a MiSeq (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Placenta (M0307, New England Biolabs) and 25 U of M-MuLV Reverse Transcriptase with corresponding buffer (M0253 ...
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bioRxiv - Genomics 2021Quote: ... the same amplicons were treated in vitro with the recombinant CpG methyltransferase M.SssI (NEB). Briefly ...
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bioRxiv - Immunology 2020Quote: ... All in vitro activity assays with recombinant GST-IdeZ or IdeZ (NEB Catalog #P0770S) (1ug per reaction ...
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bioRxiv - Developmental Biology 2020Quote: ... the Lox-Stop-Lox site was removed by exposure to recombinant Cre recombinase (NEB), using the NEB standard protocol ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant plasmids were recovered by transformation of Escherichia coli NEB5α F’IQ (New England Biolabs) and selected on LB agar supplemented with 50 μg/ml Kanamycin ...
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bioRxiv - Microbiology 2023Quote: ... All S recombinants were constructed via gene fragment Assembly (New England Biolabs, catalog #: E2621S).
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bioRxiv - Neuroscience 2021Quote: The rat HA-tagged PRG-1 ORF construct was generated by Gibson Assembly Cloning from NEB (Ipswich, MA, #E5510S) of the Plppr4 ORF clone (NM_001001508.2 ...
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bioRxiv - Microbiology 2020Quote: ... The Flag-tagged spike gene was ligated to the linearized vector using the T4 DNA Ligase enzyme from NEB. The resulting plasmid ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged ORF3c mutant Q5Y was prepared by site-directed mutagenesis using a commercial kit (New England Biolabs, E0554S).
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bioRxiv - Cancer Biology 2023Quote: ... Gαi2R179C and GαoAR243H mutants were constructed using pcDNA3.1 wild-type human ee-tagged-Gαi2- and ee-tagged-GαoA plasmids as template (cDNA Resource Center) and Q5 Site-directed mutagenesis kit (New England BioLabs). The lentiviral vector for tetracycline-inducible expression of the Gαi2R179C and GαoAR243H mutants was constructed by first cloning Gαi2R179C and GαoAR243H from pcDNA3.1 to pENTR vector (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of immunopurified GST-tagged WRN fragment was phosphorylated in vitro by Casein Kinase II (New England Biolabs) in the presence of 1X NEBuffer (50mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... All truncated or tagged versions of clones were generated using Q5 high-fidelity DNA polymerase (New England Biolabs #M0515) and NEB-Gibson Assembly (New England Biolabs #E2611S) ...
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bioRxiv - Biochemistry 2024Quote: ... 0,5 μM fluorescently tagged 3’ adapter (MultiplexDX) were ligated with T4 Rnl2(1–249)K227Q (M0351, New England Biolabs) overnight at 4°C and washed three times with PNK/ligation buffer ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 μM fluorescently tagged 3’ adapter (MultiplexDX) were ligated with T4 Rnl2(1–249)K227Q (M0351, New England Biolabs) overnight at 4°C and washed three times with PNK/ligation buffer ...
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bioRxiv - Cell Biology 2020Quote: ... PLCγ1 nSH2-cSH2-SH3 and truncations were labeled with CoA 647 (NEB, S9350) via an N-terminal ybbR tag ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were 5′-labeled with 32P-γ-ATP using T4 polynucleotide kinase (NEB) and purified on P-30 spin columns (Bio-Rad) ...
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bioRxiv - Biophysics 2021Quote: ... A dT50 oligo was radioactively labeled with 32P by T4 PNK (NEB M0201). EMSAs were performed in Imaging Buffer at room temperature ...
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bioRxiv - Cell Biology 2019Quote: Cy5 labeled luciferase RNA was produced according to the manufacturers instructions (NEB, E2040S) labeled with 6% Cy5-UTP (PA53026 ...
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bioRxiv - Biochemistry 2022Quote: DNA or RNA was labeled at 5′-termini with T4-Polynucleotide kinase (NEB) using ψ-P32-ATP as indicated in Figure 2 ...
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bioRxiv - Molecular Biology 2019Quote: Synthesized guide RNAs were 5’-labeled with 32P using T4 PNK (NEB #M0201) and [γ-32P]ATP (Perkin Elmer #BLU002A250UC ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Desialylation of ACE2-wt-Fc was performed with 2500 U mL-1 neuraminidase (New England Biolabs) in 50 mM sodium citrate (pH 5.0 ...
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bioRxiv - Microbiology 2019Quote: In vitro transcribed 3X-FLAG tagged manY transcripts – wild-type or Δenh (1 pmol) were translated in vitro using the PURExpress translation kit (NEB). Translation reactions were stopped by adding Laemmli sample buffer (Bio-Rad) ...
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bioRxiv - Biochemistry 2019Quote: ... Quantification of the ratio of MS2-tagged rRNA to WT rRNA was done via semi-quantitative PCR with Q5 DNA polymerase (NEB). Primers MS2_quant_F/R bind outside the MS2 tagged region of 23S cDNA ...
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bioRxiv - Biophysics 2021Quote: Wild-type MukB was 6×His-tagged at the C-terminus and was expressed from plasmid Pet21 in C3013I cells (NEB). For Immobilized His6-tagged MukB ...
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bioRxiv - Microbiology 2021Quote: The coding sequence of CTSL was tagged with a 3’V5 and cloned into CSIN using NEBuilder HiFi DNA Assembly (NEB) with primers CTSL_3’_V5_F ...
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bioRxiv - Plant Biology 2021Quote: Blunt ended Myc tagged GUX and AtMAGT1 CDSs were amplified from a synthetic construct (synthesised by Genewiz) with Q5 DNA Polymerase (NEB) using primers detailed in Table S1 ...
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bioRxiv - Plant Biology 2021Quote: ... SPL8 and SPL15 were amplified from cDNA to generate the respective tagged constructs using Q5 high fidelity DNA polymerase (NEB). The primers (listed in Table 5 ...
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bioRxiv - Microbiology 2021Quote: ... harboring the p6- GLuc / p6 genomes or pTM-ORF1 vectors and epitope-tagged inserts were restricted by the suitable restriction enzymes (Table 1) and subsequently ligated using the T4 DNA ligase (NEB). Finally ...
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bioRxiv - Genetics 2020Quote: ... and T3 (5’-AATTAACCCTCACTAAAGGG-3’) promoter-tagged PCR fragment from each gene using corresponding T7 and T3 RNA polymerase (T3:M0378S; T7:M0251S, BioLabs). Primers used for PCR are listed in Supplemental table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... To remove RNA secondary structure and anneal the mRNA capture primer 1 μL of tagged random hexamer (100 μM) and 0.5 μL of 10 mM dNTPs (dNTP solution set NEB - N0446S) were added to the sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids and siRNAs Bacterial expression constructs for His6-tagged p97 fragments were generated by Q5 Site-Directed mutagenesis (New England Biolabs) of pET15b-His6-p97 plasmid ...
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bioRxiv - Microbiology 2023Quote: ... brucei BFs CN PIP5Pase exclusively expressing V5-tagged PIP5Pase WT or mutant D360A/N362A for 24 h using the magnetic mRNA isolation kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the SNAP-tagged fragment was assembled into a ApaI digested MBO2-HA plasmid using NEBuilder (New England Biolabs, Ipswich, MA). The final N-SNAP-MBO2-HA construct encodes an MBO2 polypeptide with a SNAP tag at its N-terminus and a 2-HA tag located between amino acids 885 and 886 of the original MBO2 sequence (Supplemental Figure S1).
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bioRxiv - Cancer Biology 2024Quote: ... Then the ORF of the ZNRF3 short variant replaced RNF43 and was assembled into the C-terminal FLAG-tagged vector using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and the GeneArt Gibson Assembly HiFi Master Mix (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3xFLAG- and HA-tagged ZNRF3 expression vectors were generated with the Q5 Hot Start High-Fidelity DNA Polymerase Kit (New England Biolabs) by replacing the FLAG-tag ...
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bioRxiv - Biochemistry 2023Quote: Purified CBC or CBC-ARS2147-871 and their mixtures with different His-MBP-tagged partners were loaded onto Amylose resin columns (NEB). Columns were then extensively washed with a buffer containing 100 mM Tris pH 8 ...
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bioRxiv - Immunology 2021Quote: ... The recombinant plasmids (LentiCRISPRv2.gRNA10, LentiCRISPRv2.gRNA11) were then transformed into competent Escherichia coli (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant plasmid was generated by a NEBuilder® HiFi DNA Assembly (New England Biolabs) reaction ...
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bioRxiv - Cell Biology 2020Quote: Recombinant MBP fusions to Nud1 or Nud17A were expressed from pMAL-c4X (New England Biolabs). Induction in Rosetta 2 cells (Novagen ...
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bioRxiv - Cancer Biology 2021Quote: Trastuzumab deglycosylation was achieved by adding 1.1 units of recombinant PNGaseF enzyme (New England BioLabs) per 1 μg of antibody ...