Labshake search
Citations for New England Biolabs :
51 - 100 of 672 citations for Recombinant Human Alpha Fetoprotein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... coli NEB 5-alpha (New England Biolabs) was used as a cloning strain ...
-
bioRxiv - Biophysics 2023Quote: ... coli 5-alpha cells (New England BioLabs). Following mutagenesis for fluorescent labeling and/or creating RTT mutations using the Q5 mutagenesis kit (New England BioLabs) ...
-
bioRxiv - Biochemistry 2023Quote: Escherichia coli (NEB 5-alpha, NEB C2987H) were grown in 5 ml LB broth at 37°C and shaking at 180 rpm until the culture reached OD600 0.7 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... coli NEB 5-alpha (New England Biolabs) was used for construction and propagation of plasmids ...
-
bioRxiv - Synthetic Biology 2023Quote: ... coli NEB 5-alpha (New England Biolabs) and LB Miller medium (Fisher ...
-
bioRxiv - Biochemistry 2023Quote: Escherichia coli (NEB 5-alpha, NEB C2987H) were grown in 5 ml LB broth at 37°C and shaking at 180 rpm until the culture reached OD600 0.7 ...
-
bioRxiv - Biophysics 2023Quote: ... coli cells (NEB® 5-alpha, NEB) as per the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2023Quote: ... coli cells (NEB® 5-alpha, NEB) as per the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... for 1 h at room temperature before being incubated with 1.0 µg/mL of recombinant human histone H3.1 (New England Biolabs, #M2503S) for 1 h at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: Biotinylation of SNAP tagged proteins and Avi-tagged proteins were performed as suggested by manufactures (NEB, Avidity). Direct biotinylation of proteins for example-YenB ...
-
bioRxiv - Molecular Biology 2020Quote: ... NEB 5-alpha F’Iq cells (New England Biolabs) were used as the recipient strain for all plasmid constructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Escherichia coli NEB- 5-alpha (New England Biolabs) was used for cloning and BL21(DE3 ...
-
bioRxiv - Synthetic Biology 2023Quote: Plasmids were transformed into BL21-alpha cells (NEB) and plated overnight at 37 □C on kanamycin LB plates (50 μg/mL) ...
-
bioRxiv - Microbiology 2023Quote: ... coli strain NEB 5-alpha (New England Biolabs) (Table S5).
-
bioRxiv - Microbiology 2022Quote: ... Institut Jacques Boy), recombinant human IL-2 (rhIL2, 10 U/ml, Miltenyi) and DNase (1 U/ml, New England Biolabs), washed and enumerated ...
-
bioRxiv - Cell Biology 2022Quote: Recombinant human ADAMTSL2 constructs where generated by PCR-amplification with the Q5 Hot start high fidelity 2x master mix (NEB) and specific primer pairs to allow for restriction cloning ...
-
bioRxiv - Cell Biology 2023Quote: ... and 200 μM each of dATP/dCTP/dTTP at 21°C for 10 min and an additional 15 min incubation with 25 nM recombinant human RPA (a gift from Sarah W. Cai) and 0.03 U/μl T4 DNA polymerase (NEB, #M0203) at 37°C ...
-
A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Constructs expressing GFP-tagged or HA-tagged Shank3 phospho-mutants were generated using the Gibson Assembly kit (NEB) with the wild-type Shank3 as the template ...
-
bioRxiv - Cell Biology 2021Quote: ... SNAP-tagged and Halo-tagged versions of TBK1 constructs were generated by inserting SNAP (pSNAPf [New England Biolabs]) or (Halo [pHaloTag vector ...
-
bioRxiv - Neuroscience 2022Quote: All anti-tau monoclonal antibodies were generated by the hybridoma approach against recombinant tau aggregates (human full-length tau) encapsulated in the ACM Polymersomes (ACM Biolabs, Singapore) and purified by size exclusion chromatography (SEC ...
-
bioRxiv - Cancer Biology 2020Quote: ... with Hi-Fi DNA builder (NEB). Primers used to amplify specific regions are described in (Supplementary Table 1) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Recombinant Cas9 protein (NEB) and sgRNA prepared by in vitro transcription were added to the mixture and incubated at 37℃ for ∼ 4h to remove rRNA ...
-
bioRxiv - Microbiology 2019Quote: ... and retransformed into chemically competent NEB 5-alpha (NEB) to improve yields ...
-
bioRxiv - Synthetic Biology 2022Quote: ... coli strains: NEB 5-alpha (NEB C2987, not authenticated), BL21-AI (ThermoFisher C607003 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... or dam+/dcm+ strain DH5-alpha Turbo (NEB C2984) to produce unmethylated or methylated plasmids ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were transformed into NEB 5-alpha F’Iq (NEB). Standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
-
bioRxiv - Bioengineering 2023Quote: ... coli strains: NEB 5-alpha (NEB, C2987; not authenticated), BL21-AI (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2023Quote: ... and transformation (chemically competent NEB 5- alpha E. coli) were performed according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... Exchange of V5/His to myc/His was performed using the Q5 site-directed mutagenesis kit (NEB #E0554) according to the manufacturer’s protocol resulting in pEF1-ZAP-S-myc/His and pEF1-ZAP-L-myc/His ...
-
bioRxiv - Genomics 2022Quote: Library E was transformed into 5-alpha E.coli (NEB, C2987H), recovered in SOC medium (NEB ...
-
bioRxiv - Microbiology 2019Quote: ... coli strains NEB 5-alpha F’lq (New England Biolabs, US) and MC1061 with plasmid pKD46 which carries ARA-inducible Red recombination system24 ...
-
bioRxiv - Systems Biology 2021Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB) and grown in liquid culture ...
-
bioRxiv - Systems Biology 2021Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB) and grown in liquid culture.
-
bioRxiv - Biophysics 2022Quote: ... Toxicity-resistant strains such as NEB 5-alpha F’Iq (NEB) and ABLE K (Agilent ...
-
bioRxiv - Microbiology 2022Quote: ... coli dam+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/ dcm− (dam−/dcm− competent E ...
-
bioRxiv - Biochemistry 2022Quote: ... T4 DNA ligase and chemically competent DH5 alpha cells (NEB); Alt-R CRISPR-Cas9 crRNA ...
-
bioRxiv - Microbiology 2021Quote: ... coli DH5-alpha cells following manufacturer’s protocol (New England Biolabs). Sanger sequencing was used to confirm plasmid construction ...
-
bioRxiv - Molecular Biology 2021Quote: ... and NEB® 5-alpha F’IQ (New England Biolabs, NEB) were used for routine cloning procedures and chemically competent E ...
-
bioRxiv - Molecular Biology 2021Quote: ... and NEB® 5-alpha F’IQ (New England Biolabs, NEB) were used for routine cloning procedures and chemically competent E ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Assembled products were heat-shock transformed to 5-alpha (NEB), TOP10 (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... Assembled plasmids were transformed into NEB 5-alpha F’Iq (NEB) and standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
-
bioRxiv - Cell Biology 2023Quote: ... NEB 5-alpha competent E.coli (NEB #C2987H, Ipswich, MA, USA) were transformed with ligated plasmid by heat shock at 42°C for 30 seconds ...
-
bioRxiv - Genomics 2023Quote: ... The library was transformed into 5-alpha electrocompetent cells (NEB), grown in liquid culture ...
-
bioRxiv - Genomics 2023Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB). The plasmid library was prepared by maxi-prep (Sigma) ...
-
bioRxiv - Bioengineering 2024Quote: ... grown in 5-alpha competent Escherichia coli (NEB, Ipswich, MA) and purified using the PureYield MidiPrep System (Promega ...
-
bioRxiv - Immunology 2023Quote: ... via recombinant neuraminidase (NEB P0720L) as described by the manufacturer ...
-
bioRxiv - Immunology 2021Quote: ... or Amylose resin (MBP-tagged fragments, New England Biolabs) following the manufacturer’s protocols.
-
bioRxiv - Molecular Biology 2021Quote: ... Escherichia coli competent cells NEB 5-alpha (C2987, New England Biolabs), NEB Stable (C3040 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were transformed into either competent NEB 5-alpha F’Iq (NEB) or Epi400 (Lucigen) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and were performed using 5-alpha F’ Iq cells (NEB C2992H).