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Citations for New England Biolabs :
51 - 100 of 2569 citations for Recombinant E. coli MDH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were induced in SHuffle Express strains (New England Biolabs) upon addition of 1mM IPTG in cultures containing kanamycin 50 μg/mL and incubated with agitation at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The transcription template for RNAI was produced by using p770 plasmid (from E. coli strain LK_2385) as template for PCR amplification using Q5 polymerase (NEB) and LK_3413 and LK_3414 primers to introduce a T7 class II promoter (Supplementary table 3) ...
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bioRxiv - Molecular Biology 2022Quote: The recombinant pET6xHN-N constructs were transformed into Escherichia coli strain BL21(DE3) (New England Biolabs, MA, USA). The transformed clones were cultured at 37 °C in LB medium with 100 μg/mL Carbenicillin and were induced by adding 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Biophysics 2021Quote: RecA protein from Escherichia coli was purchased from New England BioLabs (NEB), Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... coli for protein purification were derivatives of pMal-c5X (New England Biolabs), which carries an AmpR marker and uses an IPTG-inducible tac promoter to expresses recombinant proteins with a factor Xa cleavable maltose-binding protein (MBP ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were expressed in Escherichia coli T7 Express (New England Biolabs) and grown in Luria-Bertani medium ...
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bioRxiv - Biochemistry 2020Quote: Proteins have been expressed in Escherichia coli strain BL21 (DE3) (NEB # C2527). In brief ...
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bioRxiv - Developmental Biology 2021Quote: ... They were then incubated overnight at 37 °C in Hybridization Buffer (10% Formamide, 10% 20x SSC, 400 µg/ml E. coli tRNA (New England Biolabs), 5% dextran sulfate ...
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bioRxiv - Microbiology 2021Quote: ... Capped mRNAs were again purified by LiCl4 precipitation followed by the addition of polyA tails using E coli Poly(A) polymerase (NEB) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: The recombinant constructs (pETDuet-1-TaPHB-1 and pETDuet-1-TaPHB-1-RUVBL1 were separately transformed into E. coli Lemo21(DE3) (NEB) chemical competent cells ...
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bioRxiv - Immunology 2023Quote: ... Unincorporated primers were digested from the pre-amplified samples using 16 U/μl Exonuclease I (E. coli, New England Biolabs) at 37°C for 30 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 µg of purified recombinant WRN protein was treated with Lambda Protein Phosphatase (LPP, New England Biolabs) in 1X PMP buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... the recombinant proteins were purified by amylose resin (#E8022S, New England Biolabs) according the manual instructions ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GFP-fusion proteins were purified from BL21(DE3) cells (NEB, # C2527H) using an N-terminal H6-tag ...
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bioRxiv - Biochemistry 2020Quote: All proteins were expressed in Escherichia coli NiCo21 (DE3) cells (New England BioLabs). To co-express multiple proteins ...
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bioRxiv - Cell Biology 2024Quote: Proteins were expressed in Escherichia coli T7 Express lysY/Iqcells (New England Biolabs) in 2×TY medium at 37°C to an OD600 of 0.8–1.2 before inducing protein expression by addition of 0.4 mM isopropyl β-D-thiogalactopyranoside (IPTG) ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were affinity-purified using amylose resin using the manufacturer’s protocol (NEB) and eluted with 20 mM maltose ...
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bioRxiv - Molecular Biology 2021Quote: ... The recombinant protein was sequentially purified using an amylose column (New England Biolabs) followed by a nickel-nitrilotriacetic acid column (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... gap fill enzymes were added (1U Sulfolobus DNA Polymerase IV NEB cat# M0327S and 10U E. coli DNA Ligase NEB cat# M0205L) and incubation continued for an additional 30min at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
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bioRxiv - Biochemistry 2022Quote: ... All other proteins were expressed in Escherichia coli BL21(DE3) cells (New England BioLabs).
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bioRxiv - Plant Biology 2019Quote: ... Recombinant proteins were purified using gravity flow columns with amylose resin (New England Biolabs). MBP cleavage was performed by incubation in cleavage buffer (50 mM Trizma-HCl ...
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bioRxiv - Neuroscience 2022Quote: Recombinant proteins were labeled with SNAP-Surface 549 dye (New England BioLabs, Cat# S9112S) for visualization ...
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bioRxiv - Plant Biology 2022Quote: ... For recombinant protein expression we used GW versions of pMAL-C2 (New England Biolabs) and pDEST-17 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant proteins were affinity purified through GST tag binding to amylose resin (BioLabs) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... coli (NEB) accomplished by heat shock at 42°C ...
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bioRxiv - Developmental Biology 2021Quote: ... coli (BioLabs) strains ...
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bioRxiv - Developmental Biology 2020Quote: ... coli (NEB).
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bioRxiv - Biochemistry 2022Quote: ... coli (NEB) were transformed ...
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bioRxiv - Genetics 2019Quote: ... coli (NEB), by electroporation in a Bio-Rad GenePulser machine (2.0k V ...
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bioRxiv - Biochemistry 2021Quote: ... coli (NEB) using the heat shock protocol provided by the manufacturer and the transformants grown on LB agar plates supplemented with ampicillin (100 µg/mL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli (NEB) as follows ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli (NEB) by adding 10 μL to each ...
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bioRxiv - Bioengineering 2021Quote: ... coli (NEB) and purified using the PureYield MidiPrep system (Promega).
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bioRxiv - Bioengineering 2021Quote: ... coli (NEB) was suspended in 3 mL of LB and grown overnight ...
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bioRxiv - Bioengineering 2021Quote: ... coli (NEB) was scaled up to aid coverage ...
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bioRxiv - Cell Biology 2021Quote: ... coli (NEB). The reporter construct for ASAP2f was assembled into 2 different Level 1 destination vectors ...
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bioRxiv - Biochemistry 2021Quote: ... coli (NEB) were chemically transformed ...
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bioRxiv - Biochemistry 2021Quote: ... coli (NEB).
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bioRxiv - Microbiology 2021Quote: ... coli (NEB) were transformed with 3 μl of the Gibson mix and plated on four LB plates containing ampicillin (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... coli (NEB 10-beta electrocompetent cells ...
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bioRxiv - Biochemistry 2020Quote: ... coli (NEB) were co-transformed with 1 ...
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DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... coli (NEB) and purified as described in Yeeles et al ...
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bioRxiv - Bioengineering 2022Quote: ... coli (NEB).
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bioRxiv - Bioengineering 2022Quote: ... coli (NEB). Recombinant proteins were purified using Ni-NTA affinity resin (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... coli (NEB) after adding 0.25 mM IPTG for 4 hours at 28°C ...
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bioRxiv - Cancer Biology 2022Quote: ... coli (NEB).
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bioRxiv - Molecular Biology 2019Quote: ... coli (NEB) were transformed and inoculated in LB media with kanamycin and grown to an A600 of 0.6 ...
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bioRxiv - Bioengineering 2021Quote: ... coli (NEB) were used for the expression of DsbC-his ...