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Citations for New England Biolabs :
501 - 550 of 2232 citations for Rat ZFP90 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The plasmid was religated with T4 DNA ligase (New England Biolabs) and transformed into TOP10 E ...
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bioRxiv - Cell Biology 2022Quote: ... The assembled plasmid library was electroporated into electrocompetent bacteria (NEB, C3020K) according to the manufacturer’s instructions as 3 reactions ...
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bioRxiv - Developmental Biology 2022Quote: ... linearized plasmids using Not1 and Sac1 restriction enzymes (New England Biolabs), and synthesized probes using a reverse transcription kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids (S2 Table) were constructed using HiFi assembly (New England Biolabs) or BEVA modular golden-gate assembly (68 ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid-backbone PCRs were restriction digested with DpnI (New England Biolabs) following the manufacturers’ protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids and inserts were assembled using NEBuilder HiFi DNA Assembly (NEB), followed by incubation at 50 °C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... PCR products and plasmids were purified with Monarch DNA kits (NEB). Overlapping primers for amplification were designed using the NEBuilder assembly tool (https://nebuilderv1.neb.com/ ...
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bioRxiv - Microbiology 2022Quote: ... all plasmids were assembled through NEBuilder® HiFi DNA Assembly (NEB). The basic backbone plasmid pCOMA_NT-crRNA was constructed by amplifying the pCB102 ori ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid pEKE2 was linearized with SacI (New England Biolabs, U.S.A.) followed by assembling of the amplified gene via Gibson Assembly according to the manufacturer’s instructions (Gibson Assembly® Master Mix ...
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bioRxiv - Synthetic Biology 2022Quote: ... and sRNA plasmids using BsaI Type IIS Enzyme (New England Biolabs) and transformed into E ...
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bioRxiv - Microbiology 2022Quote: ... the CRISPRi plasmid backbone was digested with BsmBI-v2 (NEB #R0739L) and gel purified ...
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bioRxiv - Genomics 2022Quote: ... The re-ligated plasmids were transformed into Turbo competent cells (NEB), incubated on ice for 30 mins ...
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bioRxiv - Microbiology 2022Quote: ... The transformed plasmids were extracted using a kit (New England BioLabs). The deletion strains are based on B ...
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bioRxiv - Molecular Biology 2023Quote: ... and re-circularized the plasmid using T4 blunt-end ligation (NEB). To clone pMT-Thor-APOBEC-P2A-dsRed (pCR26 ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained yeast plasmid DNA transformed into competent cells (NEB, C2987H).
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bioRxiv - Plant Biology 2023Quote: ... The digested plasmid was then blunted using Quick Blunting kit (NEB) and re-ligated using T4 DNA Ligase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were linearized with the AscI enzyme (New England Biolabs, #R0558S) for SINV and NotI enzyme (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... The exon 1 targeting plasmid was digested with XhoI/KpnI (NEB), and the exon 7 guide was amplified with primers 22 and 23 to facilitate gibson cloning of the exon 7 guide into the same backbone ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid pool was linearised overnight with KpnI-HF (NEB, R3142), purified by phenol-chloroform precipitation then resuspended in P3 (Lonza ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were constructed using Gibson Assembly (New England Biolabs, Ipswich, MA). Regions of the plasmids constructed using PCR were verified by DNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Inserts and plasmids were digested with restriction enzymes (New England BioLabs), gel purified (GeneJet Gel Extraction Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... plasmid DNA (2 µg) was digested with BsmBI-v2 (NEB, R0739) at 55°C for 3 h ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids with the correct sequence were linearized with ScaI-HF (NEB) and transformed into the B ...
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bioRxiv - Molecular Biology 2023Quote: ... Transgenesis plasmids were generated using NEBuilder HiFi Assembly Master Mix (NEB) and primers listed in Supplementary Table 2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the plasmid was constructed using NEBuilder HiFi DNA assembly (NEB). All oligos used are available in the table below.
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... Sanger sequencing was performed to validate plasmid assembly and SmaI (NEB) digestion to monitor the integrity of the ITRs ...
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bioRxiv - Cancer Biology 2023Quote: ... The pX330+Cre plasmid was digested with BbsI (New England BioLabs) and ligated to annealed and phosphorylated sgRNA oligonucleotides (Integrated DNA Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 µg of pUPRT plasmid was linearised with AgeI-HF (NEB) (GRA59 ...
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bioRxiv - Developmental Biology 2023Quote: ... A repair plasmid was made using NE Builder (New England Biolabs) that includes a 430bp gypsy insulator sequence (Geyer and Corces 1992 ...
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bioRxiv - Biochemistry 2023Quote: ... Backbone plasmids were digested with relevant restriction enzymes (New England Biolabs). Desired DNA fragments to be inserted were amplified by PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... by digesting the plasmid with StuI and NheI (New England Biolabs) using gBlocks synthetic DNA fragments (Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmid ISceI/MCS-d1GFP-SV40/ISceI was digested with XhoI (NEB) and SmaI (NEB) ...
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bioRxiv - Immunology 2023Quote: ... Mutations were introduced in the synthesized plasmids by Q5 mutagenesis (NEB).
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bioRxiv - Cell Biology 2023Quote: ... Parental plasmid was digested by restriction enzyme DpnI (New England Biolabs) and linearized plasmid was isolated by gel purification (Zymo Research) ...
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bioRxiv - Neuroscience 2023Quote: Plasmid constructs were linearized using XbaI restriction enzyme (New England Biolabs) to create DNA templates for complementary RNA (cRNA ...
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bioRxiv - Molecular Biology 2023Quote: Plasmids were constructed using a NEBuilder HiFi DNA Assembly kit (NEB) according to manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... the plasmids were linearized by digestion with SpeI (New England BioLabs), followed by purification using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Genetics 2024Quote: ... 1 µg of plasmid was incubated with 8 U M.SssI (NEB) at 37°C for 4 h for in vitro DNA methylation ...
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bioRxiv - Genomics 2024Quote: ... Tet-puro-pLKO plasmid was digested with AgeI and EcoRI (NEB) restriction enzymes ...
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bioRxiv - Microbiology 2024Quote: ... plasmid was double digested with NdeI and SpeI restriction enzymes (NEB) followed by gel purification of the 4725 bp band using a Wizard spin column (Promega) ...
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bioRxiv - Systems Biology 2024Quote: Each tagged-ORF plasmid subpool was treated with I-SceI (NEB) in the provided buffer (at a volume of 50uL for ∼1ug hORF plasmids and 200uL for ∼4ug vORF plasmids) ...
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bioRxiv - Biophysics 2023Quote: ... The LRRC8A-GFP-PhoCl-TMEM plasmid was digested with AgeI (NEB) and EcoRI (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... All plasmids were transformed into Escherichia coli Stable3 competent cells (NEB). The identity of all plasmids was confirmed by Sanger Sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... To clone inserts into plasmids NEBuilder® HiFi DNA Assembly (NEB) was used.
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bioRxiv - Microbiology 2024Quote: ... Plasmids and inserts were assembled using NEBuilder HiFi DNA Assembly (NEB), followed by incubation at 50°C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using isothermal HiFi assembly (New England Biolabs (NEB), Ipswitch ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using isothermal HiFi assembly (New England Biolabs (NEB), Ipswitch ...
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bioRxiv - Developmental Biology 2024Quote: ... pXPR_050 plasmids were digested using the Bsmb1 restriction enzyme (NEB; R0580). The synthesized double-strand oligo pools and digested pXPR_050 plasmids were ligated using T7 ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were constructed using T4 DNA ligase (New England Biolabs) with a ratio of 3:1 of insert to backbone ...
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bioRxiv - Microbiology 2024Quote: ... linear plasmid backbone was generated by restriction digestion with SmaI (NEB). Plasmid backbone DNA was treated with DpnI (NEB ...