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Citations for New England Biolabs :
101 - 150 of 10000+ citations for Rat Vasoactive Intestinal Polypeptide Receptor 1 VIPR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... input RNA was dephosphorylated by incubating with Calf Intestinal Alkaline Phosphatase (0.3U/μl) (CIP)(NEB) at 37°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of SNAP-Surface Alexa Fluor 546 (1:1000, New England Biolabs) to DMEM F12 without serum and phenol red (21041-025 ...
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bioRxiv - Neuroscience 2022Quote: ... or the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB) (replicates 4-5 and no salt treatment ...
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bioRxiv - Molecular Biology 2022Quote: ... we used NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E7405), NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7765 ...
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bioRxiv - Genetics 2023Quote: ... using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB E7405) or the NEBNext Poly(A ...
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bioRxiv - Genomics 2022Quote: ... The pCEP4 backbone was dephosphorylated using Calf Intestinal Alkaline Phosphatase (CIP) (New England Biolabs, Cat#: M0290). The backbone and fragments of either miPSC_1_L1 or miPSC_4_L1 were combined in a single ligation reaction using T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... either 30 mM acetyl phosphate or 0.5 μl Calf Intestinal Alkaline Phosphatase (CIP) (NEB, catalog#: M0290V) was added to the solution ...
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bioRxiv - Biophysics 2022Quote: ... The vector was digested with the same enzymes and Calf Intestinal Phosphatase (New England Biolabs, USA) to dephosphorylate 5’ ends.Digested inserts were gel extracted using the QiaQuick gel extraction kit (Qiagen ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 100 µL of CIP-mix containing 10 U of calf intestinal alkaline phosphatase (New England Biolabs) in CIP-buffer were added and beads incubated for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... MARCH2 chimeras containing the TM domains from either human MARCH4 or human transferrin receptor (TR) were generated using the NEBuilder HiFi DNA assembly kit (New England Biolabs) and the primers listed in Supplemental Table S2 ...
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bioRxiv - Genetics 2020Quote: ... following rRNA depletion using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB).
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bioRxiv - Systems Biology 2019Quote: ... immunoprecipitated receptors were deglycosylated with PNGase F (New England Biolabs), before resuspending in LDS sample buffer and running on NuPAGE 4-12% Bis Tris precast gels ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: GBL phosphate (8) was incubated with 5 units of calf intestinal alkaline phosphatase (CIP, New England Biolabs) in 50 mM HEPES buffer (pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... The pBluescript plasmid was cut by EcoRV and dephosphorylated by Calf Intestinal Alkaline Phosphatase (CIP) (NEB, M0290L), then ligated with the phosphorylated PCR fragments by T4 DNA Ligase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... beads were resuspended in 200 μl CIP mix (20 units of calf intestinal alkaline phosphatase (M0290, NEB) in CIP buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 50 ul of aqueous solution were recovered and treated with 0.3 U of calf intestinal phosphatase (NEB) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and the vector was treated with Mung Bean Nuclease and Alkaline Phosphatase Calf Intestinal (New England Biolabs). The blasticidin resistance gene was isolated from a plasmid pKM808 using XhoI/SalI (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... One of each pair was treated with 0.5 μl of calf intestinal phosphatase (CIP) (New England Biolabs #M0290) before the pair were incubated at 37°C for 1 hour ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The backbones of pKB11 and pKB12 were treated with calf intestinal alkaline phosphatase (CIP) (New England Biolabs, M0525S) to prevent self ligation ...
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bioRxiv - Biochemistry 2022Quote: ... purified KRAS protein was mixed with GMP-PNP (molar ratio of 10:1 GMP-PNP:KRAS) and calf intestinal alkaline phosphatase (NEB cat# M0290, 3 units per mg of KRAS). The reaction mixture was incubated for 3 hours at room temperature and then purified by size-exclusion chromatography on a 10/300 Superdex 75 GL column (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: Blood corticosterone levels were measured using the Mouse corticosterone Enzyme-linked immunosorbent assay (ELISA) kit (BIOLABS, USA) by collecting blood from wild-type mice ...
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bioRxiv - Molecular Biology 2019Quote: Libraries were prepared with NEBNEXT rRNA Depletion kit (human/mouse/rat) and NEBNEXT Ultra II Directional RNA Library Prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... depleted with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat, NEB, Figures 6 and S6), or not depleted (Figures 2B ...
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bioRxiv - Cancer Biology 2023Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat; NEB, E6310X). rRNA-depleted RNA was converted to a library using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Microbiology 2020Quote: ... 15 μg of DNA-depleted RNA was incubated with 25 units of calf intestinal alkaline phosphatase (CIP) (#M0290, NEB) in a 50 μl reaction volume for 1 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... RNAs were precipitated in cold ethanol and 0.3 M of sodium acetate and dephosphorylated using Calf-intestinal alkaline phosphatase (New England Biolabs), according to manufacturer protocol ...
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bioRxiv - Microbiology 2019Quote: ... all restriction enzyme digested plasmids were treated with Calf Intestinal Alkaline Phosphatase (New England Biolabs, Inc., Cat. No. M0290S) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Vector DNA was dephosphorylated using quick cow intestinal phosphatase (QCIP) and ligation reactions conducted using T7 DNA ligase (NEB). Purification of DNA products was done by PCR cleanup (E.Z.N.A Cycle Pure Kit ...
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bioRxiv - Genomics 2023Quote: ... 5 µg of genomic DNA was dephosphorylated by 3 µL of Quick Calf Intestinal Phosphatase (CIP, New England Biolabs) in a total volume of 30 µL for 10 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... The 5’ ends of the CRISPR leader and array fragments were dephosphorylated using Quick Calf Intestinal alkaline Phosphatase (NEB), and the DNAs were purified away from protein using a DNA Clean & Concentrator kit (Zymo Research) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies: anti-Glucocorticoid Receptor D6H2L (1:1000 dilution, Cell Signaling, New England BioLabs #12041), anti-PAX7c (1:500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Clean RNA was first rRNA depleted using the NEBNext rRNA depletion kit (Human/Mouse/Rat) (NEB) before being prepared for sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) according to the manufacturer’s instructions with 6 µl total RNA used as input per sample ...
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bioRxiv - Molecular Biology 2019Quote: ... The 5’-hydroxyl standard was generated by treating the 5’-triphosphate/3’-FAM-labeled 25mer with calf intestinal phosphatase (NEB). The cap 0 standard was generated by treating the 5’-triphosphate/3’-FAM-labeled 25mer with Vaccinia virus capping enzyme (VCE ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were rinsed three times with NP40 lysis buffer and treated with 0.5 U/μL calf intestinal alkaline phosphatase (New England BioLabs, M0290) in dephosphorylation buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1000 ng of DNA diluted in 27 μL of nuclease-free water was dephosphorylated by the addition of 3 μL of 10X CutSmart Buffer and 3 μL of Quick Calf Intestinal Phosphatase (NEB) and then incubated at 37ºC for 10 minutes ...
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bioRxiv - Microbiology 2019Quote: ... The RNA bound to Gag was first dephosphorylated at the 5’ end with calf intestinal alkaline phosphatase (New England BioLabs, NEB) followed by T4 polynucleotide kinase-catalyzed (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... This fragment was ligated into pGEM-3Zf (+) that had been digested with BamHI and SalI and dephosphorylated with calf intestinal alkaline phosphatase (NEB). 6XHis tag was added to the N-terminus of Upf1 in pGEM-3Zf (+ ...
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bioRxiv - Biophysics 2022Quote: ... The reaction was buffer exchanged in a Microspin G-50 Column (Cytiva) before treatment with Quick Calf Intestinal Phosphatase (CIP, NEB). The plasmid was digested with AgeI ...
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bioRxiv - Immunology 2022Quote: ... Beads were washed and resuspended in NEBuffer 3 containing Calf Intestinal Alkaline Phosphatase at a concentration of 0.5 U/μl (NEB, M0290) to dephosphorylate the RNA ...
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bioRxiv - Biochemistry 2022Quote: ... Inserts were then digested according to manufacturer’s protocol with Nsi-I HF and Hind-III HF and ligated into a calf-intestinal phosphatase treated plasmid using T4 DNA ligase (New England Biolabs). The resulting recombinant plasmid contained an ampicillin resistance cassette and the riboswitch insert located upstream of the fluorescent reporter protein mNeonGreen.79 The plasmid’s transcription is controlled by the moderately strong synthetic promotor ‘proD’80 which has previously been used to study the env8 class-II Cbl riboswitch.59 ...
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bioRxiv - Microbiology 2023Quote: Lysates of U2OS cells infected with wild-type HSV-2 186 at an MOI of 3 for 24 h were treated with calf intestinal alkaline phosphatase (CIP) (New England BioLabs) as described previously60.
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bioRxiv - Cancer Biology 2023Quote: ... library was prepared using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). The RNA-Seq libraries were sequenced on NextSeq 500 using the NextSeq 500/550 High Output Kit v2.5 (75 cycles ...
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bioRxiv - Microbiology 2021Quote: ... and rat (New England Biolabs), following the manufactures instructions.
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bioRxiv - Molecular Biology 2022Quote: ... We then carried out rRNA depletion with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat NEB # E6310S) using the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
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bioRxiv - Biochemistry 2021Quote: ... nucleoplasm fractions (100 µg) were incubated with either mock (distilled water) or 50 Units of calf intestinal alkaline phosphatase (QuickCIP, NEB M0525L) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... nucleoplasm fractions (100 µg) were incubated with either distilled water (mock) or 50 Units of calf intestinal alkaline phosphatase (QuickCIP, NEB M0525L) and brought up to 100 uL with PBS ...
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bioRxiv - Microbiology 2019Quote: ... The RNA bound to Gag was first dephosphorylated at the 5’ end with calf intestinal alkaline phosphatase (New England BioLabs, NEB) followed by T4 polynucleotide kinase-catalyzed (NEB ...
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bioRxiv - Immunology 2023Quote: ... 3000 ng of high molecular weight DNA was prepared by blocking available DNA ends with calf intestinal phosphatase (NEB Cat #M0525). DNA and dA-tails on all available DNA ends were cleaved by RNPs and Taq polymerase (New England Biosciences Cat #M0273 ...