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Citations for New England Biolabs :
651 - 700 of 2232 citations for Rat SEPT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: Plasmid px458 was digested with restriction endonuclease BbsI (New England Biolabs, R0539S), by addition of 1μl enzyme to 1μg plasmid ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids verified by Sanger sequencing were linearized with AseI (NEB, Catalog # R0526S) and SspI (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... of the SARS-CoV-2 Positive Control (N gene) plasmid (NEB N2117). Sanger sequencing confirmed successful mutagenesis ...
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bioRxiv - Developmental Biology 2023Quote: ... from template DNA plasmid digested by NotI or SpeI (New England Biolabs), and were cleaned by RNeasy mini kit (QIAGEN) ...
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bioRxiv - Cancer Biology 2022Quote: ... Ligated plasmids were transformed into NEB stable cells (New England Biolabs #C3040) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The pET755 plasmid was used for Q5 polymerase-mediated (New England Biolabs) PCR amplification of the backbones that contain an ampicillin resistance cassette and unc-54 3’utr ...
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bioRxiv - Immunology 2023Quote: ... The LZRS-CAR-IRES-eGFP plasmids were overnight digested with BamHI (NEB) and purified using Zymoclean Large Fragment DNA Recovery Kit (Zymo ...
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bioRxiv - Genetics 2023Quote: ... DNA was isolated according to Monarch® Plasmid Miniprep Kit (BioLabs, USA) and the PureYield® Plasmid Midiprep Protocol System (Promega ...
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bioRxiv - Genetics 2023Quote: Plasmids were constructed using Gibson assembly (New England BioLabs Cat. No. E2611), as follows ...
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bioRxiv - Plant Biology 2023Quote: ... The plasmid construct was assembled using Gibson cloning58 (E5510S, New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Plasmid was then constructed using an NEB T4 Ligase protocol (NEB – m0202L) and transformed into competent bacteria (DH5α ...
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bioRxiv - Biochemistry 2023Quote: ... and the resultant plasmids were transformed in E.Coli BL21-DE3 cells (NEB).
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 DNA fragment plasmids were digested with I-SceI (NEB) to release the overlapping fragments from the backbones ...
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bioRxiv - Biochemistry 2023Quote: ... Each plasmid was then digested with EcoRI-HF and BamHI-HF (NEB) restriction enzymes ...
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bioRxiv - Systems Biology 2023Quote: ... the plasmid was digested with HindIII-HF (New England Biolabs, Ipswitch, MA) and SacI-HF (New England Biolabs ...
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bioRxiv - Biophysics 2023Quote: ... and simultaneous blunt ligation of the plasmid using T4 DNA ligase (NEB). For the N-terminal ybbR-mutant the tag was inserted between E15 and E16 in the β’-subunit ...
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bioRxiv - Cancer Biology 2023Quote: ... fragments and destination plasmid were digested using the respective restriction enzymes (NEB) and ligation (T4 DNA Ligase ...
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bioRxiv - Microbiology 2023Quote: ... the plasmids were transformed into SHuffle T7 Express (New England Biolabs, USA) containing the plasmid pRARE extracted from Rosetta-gami B(DE3 ...
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bioRxiv - Cancer Biology 2023Quote: ... as well as on the website for the original pLentiCRISPRv2 plasmid (Addgene #52961).48,49 Plasmids were transformed into NEB Stable bacteria (New England Biolabs, MA) and colonies were screened for sgRNA inserts by Sanger sequencing of the ligation site ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid libraries are generated using Golden Gate Assembly Kits (NEB, BsaI-HFv2). The reactions undergo a cycling protocol of 30 alternating 5-minute 37°C and 16°C cycles followed by a final 60°C 5-minute hold ...
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bioRxiv - Synthetic Biology 2024Quote: ... The resulting helper plasmids were transformed into DH5α competent cells (NEB, C2987H), yielding four individual helper cell lines (M13cp-dg1 ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid pAM089 was linearized by cleavage with EcoRI-HF (New England Biolabs), separated on a 1x TBE agarose gel and extracted by electroelution ...
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bioRxiv - Biochemistry 2024Quote: ... Parental plasmids were digested by adding 5 µL 10x cutsmart buffer (NEB), and 1 µL DpnI (NEB ...
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bioRxiv - Cell Biology 2024Quote: Plasmids were transformed in BL21 (DE3) competent Escherichia coli (New England Biolabs) or Rosetta (DE3 ...
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bioRxiv - Molecular Biology 2024Quote: ... The oligonucleotides (60nM) were hybridized to φX174 ssDNA plasmids (5.3 kb, NEB) (20nM ...
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bioRxiv - Genetics 2023Quote: ... which were subsequently inserted into the plasmid backbone using NEBuilder (NEB, E2621). The purifications for PALS-C steps were done with NucleoSpin Gel and PCR Clean-Up kit (Takara ...
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bioRxiv - Neuroscience 2021Quote: ... The polymerase chain reaction (PCR) was performed on a genomic rat DNA template using a Taq PCR Kit (New England Biolabs), and the subsequent PCR product was purified using a Qiagen PCR Purification Kit (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: Libraries were prepared with NEBNEXT rRNA Depletion kit (human/mouse/rat) and NEBNEXT Ultra II Directional RNA Library Prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Genomics 2021Quote: ... or by ribodepletion with NEBNext Human/Mouse/Rat rRNA Depletion kit followed by library construction using the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Illumina 2×151 paired end reads were generated either on the HiSeq 4000 or NovaSeq 6000 sequencing platforms (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... 500ng input RNA was treated using the NEBNext Human/Mouse/Rat rRNA Depletion kit and libraries were prepared following the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Paired end 2×151 bp reads were produced using the HiSeq 4000 platform (Illumina) ...
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bioRxiv - Cancer Biology 2022Quote: ... rRNA depletion was performed using a NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and RNA was purified using Agencourt RNAClean XP Beads (New England Biolabs). Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... The ‘601’ fragments were released from the plasmid by digestion with EcoRV (NEB), isolated by incremental PEG precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ug plasmid were digested with 100 U Notl-HF and XbaI (NEB) for 7 h at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... mRNA was generated from the linearized plasmids with the T3 RNA polymerase (NEB). 200 pg of wild-type or mutant pola2 mRNA were injected into embryos from a cross at the 1-cell stage ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting plasmid was injected into zebrafish zygotes together with IsceI meganuclease (NEB) as previously described (Pyati et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid expressing EBFP2-14-3-3 theta was created using HiFi Cloning (NEB) of 14-3-3 theta into pEBFP2-C1 (Addgene plasmid #54665) ...
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bioRxiv - Developmental Biology 2021Quote: ... The plasmids were linearized using BamHI-HF (New England Biolabs, cat no. R3136L), column-purified (Qiagen ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasmids were linearized using either NcoI and SpeI restriction enzymes (NEB, Ipswich, MA). Riboprobes were synthesized from the plasmids by in vitro transcription using either T7 or Sp6 polymerase and a digoxigenin (DIG ...
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bioRxiv - Developmental Biology 2021Quote: ... the hsp70l-ephrinB2a-p2A-Venus or hsp70l-venus plasmid and I-SceI (NEB) were co-injected into the blastomeres at the one-cell stage of Tg(kdrl:DenNTR ...
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bioRxiv - Molecular Biology 2021Quote: ... A plasmid encoding Exonuclease V (RecBCD) (New England Biolabs, Inc., Ipswich, MA, USA) was used as a template to amplify recB gene as three overlapping PCR fragments B1 (3080bp) ...
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bioRxiv - Microbiology 2020Quote: ... the source plasmid (p-retronWT) was mutagenized through PCR using a kit (NEB; Q5-Site-Directed Mutagenesis Kit ...
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bioRxiv - Immunology 2021Quote: ... The parental caspase-1 plasmid (methylated) was digested using DpnI (NEB; Cat,# R0176S). Plasmids were transformed in E.coli DH5α competent cells (NEB ...
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bioRxiv - Molecular Biology 2021Quote: All mRNA generating plasmids were digested with PvuII-HF (NEB R[Δ4]151L) and ApaLI-HF (NEB R0507L ...
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bioRxiv - Molecular Biology 2021Quote: ... the minigene plasmid library was digested with EcoRI and HindIII (New England Biolabs) and run on an agarose gel ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... the resulting plasmid library and gBlock were digested with Esp3I (New England BioLabs) and gel purified ...
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bioRxiv - Evolutionary Biology 2019Quote: ... CPS mutant plasmids were constructed using the Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Bioengineering 2019Quote: ... and GA-SNAP-fwd/GA-SNAP-rev (template plasmid pSNAPf, New England Biolabs).
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bioRxiv - Biochemistry 2019Quote: ... amplified and cloned into the pHis17 plasmid using Gibson Assembly (New England Biolabs), with a stop codon before the C-terminal hexahistidine tag on the plasmid ...
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bioRxiv - Biochemistry 2019Quote: The plasmid containing the construct was linearized with Not-I HF enzyme (NEB) at 37°C for 2 h ...