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Citations for New England Biolabs :
1 - 50 of 10000+ citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We mutated aspartic acid 3420 to alanine (D3420A) and glutamic acid 3320 to alanine (E3320A) using a Q5 site-directed mutagenesis kit (E0552S, New England Biolabs, Ipswich ...
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bioRxiv - Cell Biology 2020Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... DNA substrates were 5’ terminally labelled with T4 Polynucleotide Kinase (NEB, M0201S) and ATP-γ-32P (PerkinElmer ...
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bioRxiv - Developmental Biology 2020Quote: ... Kinase assays were performed using recombinant murine c-Abl (NEB). The amounts of GST-fusion protein to add to the reaction were calibrated using western blots of the purification products with mouse anti-NICD (1:1000 ...
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bioRxiv - Microbiology 2022Quote: Chosen residues were substituted with alanine using Q5® Site-Directed Mutagenesis Kit (NEB) according to supplier protocol ...
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bioRxiv - Biochemistry 2021Quote: ... RNA substrates were 5’ end labeled using γ32P-ATP and T4 Polynucleotide Kinase (NEB, MA)38 ...
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bioRxiv - Biochemistry 2021Quote: RNA substrates (listed in Table S1) were either 5’ labelled by T4 polynucleotide kinase (NEB) and 5’ 32P-γ −ATP ...
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bioRxiv - Synthetic Biology 2023Quote: ... To prepare the TEV substrate two oligos were annealed using T4 Polynucleotide Kinase (NEB#M0201S) and T4 DNA ligase buffer (NEB#B0202S) ...
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bioRxiv - Immunology 2019Quote: ... Cysteine-to-alanine mutations were then introduced using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... the start codon was mutated to alanine using the Q5 Site-Directed Mutagenesis Kit (NEB). For C-terminal tagging ...
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bioRxiv - Synthetic Biology 2021Quote: ... annealed by temperature decrease from 95 °C to 4 °C and phosphorylated using T4 Polynucleotide Kinase (NEB, M0201) according to manufactures’ protocol ...
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bioRxiv - Cell Biology 2022Quote: ... B and C oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB, Cat #M0201L) according to the manufacturer recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was phosphorylated for 1h at 37°C with T4 Polynucleotide kinase (NEB or made in-house by the Molecular Biology Service ...
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bioRxiv - Physiology 2022Quote: ... O-GlcNAc site were mutated into alanine with Q5® Site-Directed Mutagenesis Kit (NEB#E0554). Lentivirus were packed as previously described (Huang et al. ...
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bioRxiv - Systems Biology 2019Quote: ... Each of the four-nucleic acid substrates were radiolabeled with [γ-32P]-ATP using T4 Polynucleotide Kinase (NEB). Free nucleotide was removed using G-25 MicroSpin columns (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... and Nico (λDE3) with reduced histidine-rich background proteins (NEB). The RNase I precursor contains a signal peptide (amino acid residues 1-23 ...
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bioRxiv - Biophysics 2023Quote: Oligonucleotides used as substrates in D-loop assays were radiolabelled at their 5′-end using T4 Polynucleotide Kinase (T4 PNK - NEB) and adenosine triphosphate [γ-32P] (Perkin Elmer ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20th of the annealed substrates was 5′-end-labelled with [γ-32P]-ATP and T4 polynucleotide kinase (New England Biolabs). For fluorescently labeled HJ40 substrates ...
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bioRxiv - Microbiology 2023Quote: ... The catalytic residue H528 of esaD was mutated to encode an alanine using a Q5 site-directed mutagenesis kit (NEB). esxCBED amplified from COL genomic DNA was then inserted upstream of esaD by HiFi assembly ...
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bioRxiv - Microbiology 2023Quote: ... which was created by substituting the codon encoding cysteine at amino acid residue position 2 on HypC with a codon decoding as alanine using site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs) employing the oligonucleotides HypCfwd (5’-TATACATATGGCGATAGGCGTTCCCGG-3’ ...
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bioRxiv - Cell Biology 2024Quote: ... site-directed mutagenesis of the codon encoding lysine at amino acid position 41 was altered to alanine using the Q5 site-directed mutagenesis kit (New England Biolabs) using pPM11 as a template ...
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bioRxiv - Genomics 2020Quote: ... T4 Polynucleotide Kinase (NEB) treatment is performed at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... T4 polynucleotide kinase (NEB) was used to phosphorylate the 5’ end of the 3’ tRNA half and was heat inactivated ...
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bioRxiv - Neuroscience 2021Quote: ... T4 Polynucleotide Kinase (NEB) was used to catalyze the addition of 5’ monophosphate and removal of the 3’ phosphate in the RNA fragments to leave a 3’ hydroxyl terminal needed for adapter ligation ...
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bioRxiv - Molecular Biology 2020Quote: ... + T4 polynucleotide kinase (NEB)) ...
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bioRxiv - Molecular Biology 2021Quote: ... T4 Polynucleotide Kinase (NEB) 5’ radiolabelled oligonucleotides were used for detection of mitochondrial transcripts (MT-ATP8/6 5’-TGGGTGATGAGGAATAGTGTAAGGAG ...
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bioRxiv - Genetics 2020Quote: ... T4 Polynucleotide Kinase (NEB) was used to phosphorylate the forward and reverse sgRNA during the annealing ...
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bioRxiv - Molecular Biology 2022Quote: ... polynucleotide kinase (NEB, M0201S) and T4 DNA ligase (NEB ...
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bioRxiv - Plant Biology 2022Quote: ... T4 polynucleotide kinase (NEB), and Klenow Fragment (30/50 exo- ...
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bioRxiv - Molecular Biology 2022Quote: ... and polynucleotide kinase (NEB) to repair the 5’ end ...
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bioRxiv - Genomics 2022Quote: ... + T4 polynucleotide kinase (NEB)) ...
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bioRxiv - Biochemistry 2023Quote: ... by polynucleotide kinase (NEB) in 1x PNK buffer at 37°C for 45 minutes ...
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bioRxiv - Microbiology 2019Quote: ... and phosphorylated for 30 minutes at 37 °C with 4U of T4 Polynucleotide Kinase (New England Biolabs) in a total volume of 20 µl according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Radiolabeling of oligonucleotides was carried out for 1 h at 37 °C using T4 polynucleotide kinase (NEB), 0.25 μM oligonucleotide ...
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bioRxiv - Biochemistry 2024Quote: ... the concentrated protein at 3 mg/mL was incubated with c-AMP Protein Kinase A PKA (NEB) (molar ratio of 40:1 ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Cell Biology 2020Quote: All in vitro kinase assays were done in NEBuffer for protein kinase (NEB) at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... The OprCAA mutant was produced by changing the key amino acids Cys143 and Met147 to alanine residues using the KLD Quickchange site-directed mutagenesis kit (New England Biolabs, UK) and specific primers containing both mutation sites (forward ...
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bioRxiv - Cell Biology 2023Quote: ... The C-KSR-GS construct replacing the vector-based linker (LELKLRILQS) by a glycine-serine rich linker (GGSSGGGGA) was generated by site directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, USA) of the C-KSR-EGFP plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... and T4 Polynucleotide kinase (NEB). Samples were cleaned up using MyONE Silane beads and RNA was isolated with RLT buffer (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... and T4 polynucleotide kinase (NEB) and labeled substrates were purified by native polyacrylamide gel electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... After T4 polynucleotide kinase (NEB) treatment ...
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bioRxiv - Cancer Biology 2019Quote: ... T4 DNA polynucleotide kinase (NEB), Klenow (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... 50U T4 Polynucleotide Kinase (NEB) and 25U Klenow fragment DPol I (NEB ...
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bioRxiv - Genomics 2021Quote: ... T4 Polynucleotide kinase (NEB M0201S). Adenylation was performed with 3’-5’ Klenow Fragment (NEB M0212L) ...
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bioRxiv - Bioengineering 2021Quote: ... and T4 polynucleotide kinase (NEB). Intramolecular circularization of the DNA was performed with T4 DNA ligase (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... using T4 polynucleotide kinase (NEB) and γ-ATP (PerkinElmer) ...
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bioRxiv - Biochemistry 2020Quote: ... using T4 polynucleotide kinase (NEB).
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bioRxiv - Biochemistry 2021Quote: ... using T4 polynuclotide kinase (NEB) and cleaned using ssDNA/RNA Clean and Concentrator kit (Zymo Research D7010).