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Citations for New England Biolabs :
51 - 100 of 8741 citations for Rat IL 18 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... rRNA depletion and library preparation for sequencing was completed with the NEBNext protocol (‘Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared according to the protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) ...
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bioRxiv - Immunology 2023Quote: ... Ribosomal RNA from 1000 ng total extracted RNA was depleted using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat; New England Biolabs Inc.). The remaining RNA was used to produce the sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2021Quote: DNAse-treated RNA with high RIN value was used to deplete ribosomal RNA using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6350) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... ribosomal RNA depletion was carried on the extracted RNA using Nebnext rRNA depletion kit (Human/mouse/rat) (New England BioLabs. In, USA). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared from RNA samples (150ng) using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB Cat# E7405) in conjunction with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB Cat# E7765) ...
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bioRxiv - Biochemistry 2023Quote: ... An mRNA transcript library for Illumina sequencing was created using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs, Ipswich, MA). A NextSeq 500 sequencer (Illumina ...
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bioRxiv - Immunology 2023Quote: ... The other pCMVtag2B vectors containing different truncated versions of rat ITCH (Fig 2F) were generated via Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Cat#E0554S) by circularizing the PCR products amplified with the primers listed in Table S2 ...
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bioRxiv - Microbiology 2020Quote: ... pET20b::bepA::6xHis vector was used in 18 cycles of PCR using the Phusion polymerase (NEB) as described by the manufacturer ...
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bioRxiv - Microbiology 2019Quote: ... pET20b::bepA::6xHis vector was used in 18 cycles of PCR utilising the Phusion polymerase (NEB) as described by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using phenol-chloroform and subjected to ribosomal RNA removal using a NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB, Ipswich, MA). A RNAseq library was prepared by using a NEBNext® Ultra directional RNA library prep kit (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... and (iii) using 18 cycles of PCR amplification with Phusion High Fidelity DNA Polymerase (New England Biolabs). Libraries were pooled and sequenced (paired-end 2×150bp ...
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bioRxiv - Biophysics 2021Quote: ... Complexes were prepared by incubating spike ectodomain preparations with either ACE2 (residues 18–615, New England Biolabs), VH ab8 ...
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bioRxiv - Microbiology 2022Quote: ... the above construct was amplified using primers 17 and 18 and integrated into BamHI/PacI-digested (NEB) pUPRT plasmid by Gibson assembly ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixed RNA was depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) and fragmented using NEBNext® Magnesium RNA Fragmentation Module (NEB, cat. no. E6150S) for 5 min ...
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bioRxiv - Immunology 2020Quote: ... and on the Sprague Dawley (SD) strain (rats) (Hera Biolabs) were obtained from vendor and breed in the Division of Laboratory Animal Resources (DLAR ...
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bioRxiv - Microbiology 2023Quote: ... plasmid DNA encoding rat HEV was linearized using EcoRI (NEB). Five percent of the reaction was subjected to gel electrophoresis with ethidium bromide staining and visualized with ultraviolet light to verify that linearization had occurred.
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bioRxiv - Plant Biology 2019Quote: ... the corresponding dsRNAs were processed in 18-25 nt siRNAs by ShortCut® RNase III (NEB, Ipswich, MA) for 40 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 50 μl of DNase I mixture (25 μl of 10× DNase I buffer, 18 μl of NEB DNase I ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 ng total RNA in a volume of 9 μl was ligated to 1 μl custom adapter mix (18S:28S ratio 1:2) using 1.5 μl T4 DNA ligase (New England Biolabs) in NEB next Quick ligation buffer (3 μl ...
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bioRxiv - Developmental Biology 2019Quote: ... Libraries were generated using 18 cycles of PCR amplification with NEB high fidelity 2x master mix (New England Biolabs), cleaned up with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2019Quote: ... 200 ng of total RNA samples with RQN values >6.0 were used for cDNA synthesis together with the Oligo d(T)18 mRNA Primer (NEB) and SuperScript III Reverse Transcriptase (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... First strand cDNA synthesis was done by incubating 1 μg total RNA with 2.5 mM Oligo dT(18) and 0.5 mM dNTP mix (NEB, N0447L) for 5 min at 70°C before placing on ice for 2 min ...
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bioRxiv - Plant Biology 2022Quote: ... we first created a RUBY-minus vector lacking the gene GT by assembling PCR product 1 containing CYP76AD1 and DODA and PCR product 2 containing Arabidopsis HSP18.2 terminator into a pGFPGUSplus vector 18 via NEBuilder HiFi DNA Assembly (New England BioLabs). The pAXY0006 vector of split-RUBY was generated by assembling PCR products containing f1 fragment of gene GT (named GTf1 ...
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bioRxiv - Molecular Biology 2019Quote: ... Luciferase activity was determined in a luminometer (Optocomp I) by the addition of ∼18 µl of sample to 6-7 µl diluted coelenterazine (for Gluc, NEB), or ∼20 µl of sample to 15-18 µl Fluc substrate (Promega).
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bioRxiv - Genomics 2021Quote: ... and on the other side a primer that targets a region introduced through the library preparation called “Read 1” (18 cycles using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs)) ...
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bioRxiv - Neuroscience 2020Quote: ... either purified directly (insert) or from agarose gel (vector) and ligated (16-18 h, 20-22 °C) with T4 DNA ligase (NEB) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... to the branch point recognition sequence (Nucleotides 18-42) of U2 snRNA and to GAPDH mRNA were added in combination with RNase H (NEB) to NE ...
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bioRxiv - Neuroscience 2022Quote: ... was linearized for 18 hours at 37°C in a 250 μl reaction containing restriction enzyme NheI and CutSmart buffer (New England Biolabs). The linear DNA plasmid was next used to produce complementary RNA (cRNA ...
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bioRxiv - Immunology 2022Quote: ... Sample index PCR: 2 µl tagging PCR product was mixed with 18 µl PCR mix (4 µl 5x phusion HF buffer (NEB); 0.4 µl Phusion DNA Polymerase (NEB) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR-enrichment was performed in 18 cycles in ten 10μL reactions per sublibrary with Phusion® HF PCR Mastermix (New England BioLabs) by mixing 5µl 2× Phusion Master mix ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was clarified by centrifugation at 5°C at 18 500 rpm for 30min and loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB1_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Developmental Biology 2020Quote: Alg2 cDNA was amplified with RT-PCR from the cDNA of wt Oryzias latipes Cab strain stage 18 embryos with Q5® High-Fidelity DNA Polymerase (New England Biolabs) by using primers with BamHI-HF (New England Biolabs ...
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bioRxiv - Cell Biology 2019Quote: ... 500ng of RNA from MEFs or 350ng of RNA from BMDMs were reverse transcribed using oligo(dT)18 primer (New England Biolabs, #513165) and SuperScript II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... dsDNA fragments were inserted into PCR-amplified (primers 17-18 in Table S3) and purified pRSET vector fragment by Gibson Assembly (NEB, USA) to add sequence parts necessary for cDNA display generation to the DNA library ...
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bioRxiv - Systems Biology 2021Quote: Rho libraries were amplified using primers MO574 and MO575 (Supplementary file 6) for 6 cycles at an annealing temperature of 66C followed by 18 cycles with no annealing step (NEB Phusion) and then purified with the Monarch PCR kit (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR product was denatured and annealed in an 18 μl reaction (15 μl PCR product, 2 μl NEB Buffer2 (10X), 1 μl nuclease-free H2O ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated with 25 μL of containing TF or 18S primers and fluorescent Luna® Universal qPCR Master Mix (New England Biolabs), and qRT-PCR was carried out in triplicate for each sample on the CFX Connect real-time System (Bio-Rad Laboratories).
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bioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in 20 µl of distilled water and mixed with 18 µl gel loading dye (New England Biolabs, Inc.). Samples were resolved in a 10% SDS-polyacrylamide gel ...
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bioRxiv - Cell Biology 2021Quote: ... The entire rat Cx43 sequence was removed using EcoRI (Cat. No. R3101S; NEB) and BamHI (Cat ...
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bioRxiv - Molecular Biology 2022Quote: For chitin-based sandwich ELISA 50 μl of chitin magnetic beads (New England Biolabs, Ipswich, MA, USA) were transferred into a 1.5 ml reaction tube ...
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bioRxiv - Microbiology 2020Quote: ... pCrPV-1A-DcDV was linearized by inverse PCR with primers 17 and 18 and the linearized plasmid was circularized by blunt end ligation with T4 DNA ligase (New England Biolabs, Ipswich, Massachusetts) according to the manufacturer’s instructions to create pCrPV-1A-DcDV-1A ...
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bioRxiv - Genomics 2024Quote: ... Small RNA sequencing libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina following the manufacturer’s recommendations with the 3’ ligation step changed to 16°C for 18 hours to improve capture of methylated small RNAs (New England Biolabs, cat# E7300S). Small RNA PCR amplicons were size selected on 10% polyacrylamide non-denaturing gels ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 8.0) were incubated with pre-washed pan anti-Kbhb beads (PTM Biolabs Inc., Chicago, IL) at 4 °C overnight with gentle shaking ...
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bioRxiv - Microbiology 2022Quote: ... Institut Jacques Boy), recombinant human IL-2 (rhIL2, 10 U/ml, Miltenyi) and DNase (1 U/ml, New England Biolabs), washed and enumerated ...
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bioRxiv - Neuroscience 2021Quote: The rat HA-tagged PRG-1 ORF construct was generated by Gibson Assembly Cloning from NEB (Ipswich, MA, #E5510S) of the Plppr4 ORF clone (NM_001001508.2 ...
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bioRxiv - Cell Biology 2019Quote: ... by random priming without denaturation with 4 mg random nonamer oligo (Integrated DNA Technologies, Skoie IL) and 10 units of Klenow (New England Biolabs, Ipswich, MA). Unincorporated dye was removed with Microcon columns (30 kDa MW cutoff ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation experiments were performed with 15 μL of Pan anti-glycine lysine antibody conjugated to agarose beads (PTM Biolabs, Chicago, IL, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... and then cloned into a customized minigene plasmid (a derivative of the pSpliceExpress vector)29 containing an RSV-promoter and two control exons (rat insulin exons 2 and 3) using the NEBuilder® HiFi DNA assembly (NEB, E2621). Amplified fragments were inserted between the two control exons ...
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bioRxiv - Genomics 2022Quote: Ribosomal RNA depletion kit (rRNA Depletion Kit, E6310L, NEB)