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Citations for New England Biolabs :
401 - 450 of 10000+ citations for Rat Epithelial Discoidin Domain Containing Receptor 1 DDR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 8 μL of RNA was added per section to step 2.1 through to 2.11.11 of the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of RNA was used for cDNA synthesis using the ProtoScript® II kit (New England BioLabs). qPCR reactions were carried out with FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 μg of DNA library was used as a template for mRNA synthesis by HiScribe T7 kit (NEB). The product was purified by NH4Ac precipitation and dissolved in RNAse-free water to a final concentration of 3 μg/ul.
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bioRxiv - Developmental Biology 2022Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for PCR used are as follows ...
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bioRxiv - Developmental Biology 2022Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for PCR used are as follows ...
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bioRxiv - Molecular Biology 2023Quote: CDKN1A intron 1 APA 3’ splice site was deleted using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) as per the manufacturer’s instructions using forward primer (5’-TCCCCACCCCAAAATGACGCGCAGCC-3’ ...
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bioRxiv - Molecular Biology 2023Quote: 9N_VRA3 adapter oligonucleotide (Supplementary Table 1) was pre-adenylated with the 5’ DNA Adenylation Kit (New England Biolabs) according to the manufacturer’s protocol at a 5X scale ...
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bioRxiv - Molecular Biology 2020Quote: ... The complexation reaction was carried out in a solution containing 1X NEBuffer 2.1 (NEB) (composed of 100 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... Phosphorylation reaction containing TEX264-FLAG and CK2 (mixture of CK2A1 and CK2B, P6010, NEB) with or without 1 mM CX4549 (S2248 ...
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bioRxiv - Immunology 2021Quote: ... Samples were added to second-strand synthesis mix containing 2× NEB buffer 2 (NEB), 625 nM dNTP Mixture (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... The vector (pPICZαA) containing the AfLPMO16 gene was linearized by Pme1 (New England BioLabs) and transformed to Pichia pastoris X33 competent cells ...
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bioRxiv - Plant Biology 2020Quote: ... 19 μL binding mixture containing 4 μL nuclear extracts and 5U Rnase H (NEB) or bovine pancreatic RNase A (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... in a 10 μL reaction mix containing 1X Luna Universal qPCR Master Mix (NEB) and 400 nM forward and reverse primers ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in 200 μl of MNase reaction buffer containing 2.5U of MNase (NEB, M0247) and incubated for 10 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... plasmid clones containing the correct insert were linearized with DraI (#R0129S, New England Biolabs) and sgRNA was synthesized in vitro using a Maxiscript T7 Transcription Kit (#AM1312 ...
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bioRxiv - Molecular Biology 2021Quote: ... Eighty µl of 1X PNK containing 2µl (20U) of T4 PNK (New England Biolabs), 2µl (80U ...
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bioRxiv - Cell Biology 2019Quote: ... The dUTP-containing strand was degraded using Uracil-DNA Glycosylase (New England Biolabs #M0280S). The resulting single-stranded cDNA was amplified with 13 or 15 PCR cycles followed by size-selection using Pippin Prep (Sage Science) ...
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bioRxiv - Biochemistry 2021Quote: ... Control samples containing only RNA were treated with RNA 5’ Pyrophosphohydrolase (RppH, NEB, M0356S) to convert 5’-PPP RNA into 5’-P RNA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Uracil-containing strands were degraded by treatment with USER Enzyme (New England Biolabs, USA). Sequencing was performed using Illumina Novaseq 6000 Platform in paired-end format with the Phred score of 64 ...
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bioRxiv - Biophysics 2021Quote: ... A plasmid containing two BsaI sites was first treated with BsaI (New England Biolabs), then separated on a 1% agarose gel ...
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bioRxiv - Genomics 2021Quote: ... The sgRNA-containing region was PCR-amplified with NEBNext Ultra II Q5 MasterMix (NEB), acrylamide gel-purified ...
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bioRxiv - Genomics 2020Quote: ... 0.3 uL of TSO digestion buffer containing 1U Uracil-DNA glycosylase (UDG, NEB M0280S) were added to each well ...
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bioRxiv - Genomics 2022Quote: ... A restriction digestion mix containing 7 µl of 10X NEB CutSmart Buffer (NEB #B7204), 4.5 µl of NlaIII (NEB #R0125) ...
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bioRxiv - Genomics 2022Quote: ... Illumina sequencing adapters containing uracil hairpin loop structure and 3′ T overhangs (NEB, #E7337A) were ligated to the DNA by adding 150 units of Quick T4 DNA Ligase (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... containing a His6-tagged Ulp1 gene was transformed into T7 Express competent cells (NEB) and grown in LB medium supplemented with ampicillin (100 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... using SYBR green I containing Luna® Universal qPCR Master Mix (NEB England Biolabs). The qPCR was performed using the QuantStudio 5 Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... using SYBR green I containing Luna® Universal qPCR Master Mix (NEB England Biolabs). The qPCR was performed using the QuantStudio 5 Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: ... pDR274 transcription plasmids containing the gRNA sequences were linearized with DraI (NEB; Ipswich, MA) and amplified using Accustart Taq DNA Polymerase HiFi (Quanta Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μL of a mix containing 5 U of T4 RNA Ligase 2 (NEB), 1 mM ATP ...
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bioRxiv - Developmental Biology 2023Quote: ... Injection mixtures containing 500 ng/µl of Cas 9 protein (NEB; Cat. no.: M0641) and 300 ng/µl of guide RNA were prepared and injected into eggs within 4 hours of egg laying ...
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bioRxiv - Neuroscience 2023Quote: ... containing 5 µl of collection buffer (NEBNext Single Cell Lysis Module, New England Biolabs). Noteworthy ...
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bioRxiv - Biochemistry 2023Quote: ... Annealed oligos containing sgRNA sequences were phosphorylated with T4 polynucleotide kinase (New England Biolabs) and ligated into linearized vector backbone ...
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bioRxiv - Cancer Biology 2023Quote: ... A volume of 40 μl reaction mix containing 5 μl isothermal amplification buffer (NEB), 3 μl 100 mM MgSO4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Annealed oligos containing sgRNA sequences were phosphorylated with T4 polynucleotide kinase (New England Biolabs) and ligated into linearized vector backbone ...
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bioRxiv - Pathology 2023Quote: ... containing 6.25 μL of Taq 2x Master Mix (New England Biolabs, Ipswich, MA, U.S.A), 0.16 μL of the forward primer with the M13 tail ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... stained in HI buffer containing 5 μM SNAP-Surface Alexa 647 (New England Biolabs) for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: The vector pKGWFS7 containing proSPPiDDR1 was digested by HindIII and MfeI restriction enzymes (NEB) and then loaded on an agarose gel ...
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bioRxiv - Biochemistry 2023Quote: ... The reactions were adjusted to 80 μL containing 1x NEBuffer r1.1 (New England Biolabs) and a final concentration of 1.4 nM of hRNase 4 and 0.15 U/μL of polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2023Quote: ... containing oligo pool template at 3.33 pg/μL and LAMP Fluorescent Dye (B1700, NEB) at 0.5X was aliquoted across a PCR plate ...
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bioRxiv - Molecular Biology 2023Quote: ... pRSSM1.PleII plasmid (containing N4mC at cacCgc sites) (Dr. Iain Murray, NEB Ipswich MA) were used as spike in controls in NGS deamination assay.
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bioRxiv - Immunology 2024Quote: Maxi-prepped plasmids containing the replicate barcoded libraries were digested using SalI-HF (NEB) to linearize the plasmid prior to long-read sequencing ...
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bioRxiv - Bioengineering 2024Quote: ... 500 µL PCR reagents containing 1.67X Q5® High-Fidelity Master Mix (NEB, M0515), 0.625 mg/mL BSA ...
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bioRxiv - Microbiology 2020Quote: ... were carried out using specific primers (Supplemental Table 1) and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). After sequence verification ...
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bioRxiv - Molecular Biology 2019Quote: ... Pooled PCR products (1 μg) were digested with the NEBNext dsDNA fragmentase kit (New England BioLabs, Inc., Ipswich, MA). Fragmented DNA was end-repaired with the NEBNext End Repair Module (New England BioLabs ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized from 1 μg total RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB) with oligo-dT priming according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Both fragments were run on a 1% agarose gel and purified using a Monarch DNA Gel Extraction kit (NEB). The fragments were then joined using the NEBuilder HiFi DNA Assembly protocol (NEB).
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of total RNA was used in cDNA synthesis using ProtoScript II Reverse Transcription kit (NEB, Cat # e6560) as per manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was purified from single-humanized Hsα1 and quintuple-humanized Hsα1,α2,α3,α4,α7 strains using the Monarch Nucleic acid purification kit according to the manufacturer’s protocol (NEB). Spheroplasts were obtained before the genomic DNA extraction for a high-quality DNA prep ...
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bioRxiv - Microbiology 2022Quote: ... xanthus cells grown on 1.5% agar supplemented with 1% CTwas extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB)) ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instruction ...