Labshake search
Citations for New England Biolabs :
201 - 250 of 10000+ citations for Rat Dual Specificity Mitogen Activated Protein Kinase Kinase 7 MAP2K7 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... dephosphorylated by T4 polynucleotide kinase (New England Biolabs), ligated to preadenylated linkers containing the T7 promoter region by T4 RNA ligase 2 ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by Casein Kinase II (New England Biolabs) in 1X PK buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... phosphorylation with T4 Polynucleotide Kinase (New England BioLabs), and circularization with an NEB Quick Ligation Kit (New England BioLabs) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and T4 polynucleotide kinase (New England Biolabs, #M0201S), and then ligating annealed sgRNAs and pre-digested and purified LentiCRISPRv2E vector with Quick Ligation Kit (New England Biolabs ...
-
bioRxiv - Plant Biology 2023Quote: ... and 5′ phosphorylated with T4 Polynucleotide Kinase (NEB). The fragments were mixed with Golden Gate cloning adaptors (5′ adapter ...
-
bioRxiv - Genomics 2023Quote: ... and T4 Polynucleotide Kinase (New England Biolabs, M0201L). 1uL of 1uM annealed sgRNA oligos were then cloned into 30ng Lenti-sgRNA(MS2)-puro constructs (Addgene #73797 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 units of T4 polynucleotide kinase (NEB, M0201S), 0.09 nM dNTPs and 0.045 μg/μL of BSA at 12°C for 30 min ...
-
bioRxiv - Molecular Biology 2023Quote: Purified RNA was treated with polynucleotide kinase (NEB) to prepare 5’ and 3’ ends for library construction as described for LORAX-seq.
-
bioRxiv - Cancer Biology 2023Quote: ... and 1.5ul of T4 Polynucleotide Kinase (NEB, M0201L). Sample was incubated at 37°C for 1 h and then inactivated by addition of EDTA at a final concentration of 20mM ...
-
bioRxiv - Genomics 2024Quote: ... 15 U T4 polynucleotide kinase (New England Biolabs), 5 U Klenow DNA polymerase (Roche) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 3 µl of T4 polynucleotide kinase (NEB, M0201L) and 4 µl of terminal deoxynucleotidyl transferase (Enzymatics ...
-
bioRxiv - Cancer Biology 2024Quote: ... After dephosphorylation with T4 Polynucleotide Kinase (NEB, #M0201S) the adapter Linker-1 (5’ rAppCTGTAGGCACCATCAAT/3ddC/ 3’ ...
-
bioRxiv - Cancer Biology 2024Quote: ... and T4 polynucleotide kinase (T4 PNK, NEB, M0201) was used to add a phosphate group at the 5′ ends ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 1.5ul of T4 Polynucleotide Kinase (NEB, M0201L). Samples were incubated at 37°C for 1h and then inactivated by addition of EDTA at a final concentration of 20mM ...
-
bioRxiv - Cancer Biology 2024Quote: ... and T4 polynucleotide kinase (New England BioLabs (NEB)) ...
-
bioRxiv - Cancer Biology 2024Quote: ... and T4 polynucleotide kinase (New England BioLabs (NEB)) ...
-
bioRxiv - Biochemistry 2021Quote: ... at 37 °C for 1 hour or first with 5 units T4 Polynucleotide Kinase with 25 mM ATP in 1 X T4 Kinase buffer (T4PNK, NEB, M0236S) at 37 °C for 30 min and then with XRN-1 ...
-
bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein-RNA complexes were radiolabeled on-bead in 40 µl reactions with T4 polynucleotide kinase (NEB) and 2 µl [γ-32P]ATP (6,000 Ci/mmol ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2 pmoles of ssDNA were phosphorylated with 0.7 µl of [γ-32P]-ATP (7 µCi, Hartman Analytic) using T4 polynucleotide kinase (10 U, NEB). The reaction was supplemented with 10 mM DTT and incubated for 30 min at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... The radioactive probe was prepared from 40 pmoles of primers described in Supplementary Table 7 and labeled at the 5’ end with 10 units of T4 polynucleotide kinase (New England Biolabs) and [γ- 32P]-ATP (150 µCi) ...
-
bioRxiv - Molecular Biology 2020Quote: ... RNA fragments were treated with T4 polynucleotide kinase (NEB), ligated to the linker (NEB Universal miRNA Cloning Linker ...
-
bioRxiv - Microbiology 2019Quote: ... amplified fragment was phosphorylated using T4 polynucleotide kinase (NEB) and then ligated using T4 DNA ligase (NEB) ...
-
bioRxiv - Molecular Biology 2021Quote: ... and dephosphorylated using T4 Polynucleotide Kinase (M0201, NEB, USA) prior to isopropanol precipitation and resuspension in 10 mM Tris [pH8]) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Total RNA was treated with T4 Polynucleotide Kinase (NEB) and rRNA depleted using an in-house protocol and cDNA library preparation was preceded by ultrasonic fragmentation ...
-
bioRxiv - Molecular Biology 2021Quote: ... The oligos were radiolabeled by T4 polynucleotide kinase (NEB) with adenosine [γ32P]-triphosphate (6000 Ci/mmol ...
-
bioRxiv - Genomics 2020Quote: ... 5′ end phosphorylation using T4 polynucleotide kinase (NEB, M0201L), (4 ...
-
bioRxiv - Genomics 2020Quote: ... RNA overhangs are repaired with T4 Polynucleoide Kinase (NEB) with no ATP at 37°C for 20 min ...
-
bioRxiv - Synthetic Biology 2020Quote: ... gDNAs were either phosphorylated using T4 Polynucleotide Kinase (NEB) in lab or phosphorylated guides were purchased from IDT ...
-
bioRxiv - Bioengineering 2019Quote: ... 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK, NEB), 1x CutSmart® buffer (NEB).
-
bioRxiv - Plant Biology 2021Quote: ... oligos were end-labelled using T4 polynucleotide kinase (NEB) with γ-32P ...
-
bioRxiv - Genetics 2020Quote: ... The oligonucleotides were phosphorylated with T4 polynucleotide kinase (NEB), annealed and inserted in the linearized vector with T4 DNA ligase (NEB) ...
-
bioRxiv - Microbiology 2020Quote: ... and 4 μL T4 polynucleotide kinase (New England BioLabs) in a 100 μL reaction for 2 hours and purified using P-30 spin columns (BioRad) ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 5’ end-labeled using T4 polynucleotide kinase (NEB) and [γ-32P] ATP ...
-
bioRxiv - Biochemistry 2022Quote: ... followed by KLD (Kinase, Ligase, and DpnI) digestion (NEB). Detailed primers used for TatA variant construction can be found in Supplemental Table 2 ...
-
bioRxiv - Cell Biology 2022Quote: ... T4 Polynucleotide Kinase (1:10, #MO201S, New England Biolabs), T4 Ligase reaction buffer (#B0202S ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and phosphorylated using T4 polynucleotide Kinase (New England Biolabs). The phosphorylated DNA was cleaned and concentrated again as above and then ligated using T4 DNA Ligase (New England Biolabs) ...
-
bioRxiv - Biophysics 2019Quote: ... and T4 polynucleotide kinase were also purchased from NEB. All other chemicals were purchased from Sigma-Aldrich (St ...
-
bioRxiv - Molecular Biology 2019Quote: ... [γ32P]ATP and T4 polynucleotide kinase (New England Biolabs).
-
bioRxiv - Genomics 2019Quote: ... The reverse primers are phosphorylated using Polynucleotide kinase (NEB).
-
bioRxiv - Microbiology 2021Quote: ... 1 µl 10× T4 polynucleotide kinase (PNK) buffer (NEB), 6 µl of water ...
-
bioRxiv - Cancer Biology 2021Quote: ... Insert oligonucleotides were phosphorylated using T4 polynucleotide kinase (NEB) at 37 °C for 1 hr and were annealed by heating to 95°C for 5 min and slowly cooled to room temperature ...
-
bioRxiv - Pathology 2021Quote: ... then treated with Kinase-Ligase-DpnI (New England Biolabs) prior to heat shock transformation into chemically competent DH5α Escherichia coli cells (Table 1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... T4 polynucleotide kinase (New England Biolabs, with 1mM ATP) and Terminal exonuclease (buffer A ...
-
bioRxiv - Genetics 2019Quote: ... Annealed oligos were phosphorylated by T4 polynucleotide kinase (NEB) for two hours at 37°C followed by 65°C for 20 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... T4 Polynucleotide kinase PNK 200 U/ml (NEB M0201L); DNA Polymerase I Large (Klenow ...
-
bioRxiv - Cell Biology 2022Quote: ... Oligos were phosphorylated with T4 Polynucleotide Kinase (NEB M0201) at 37oC for 30 minutes ...
-
bioRxiv - Plant Biology 2022Quote: ... Following phosphorylation with T4 polynucleotide kinase (New England BioLabs), the PCR products were ligated into expression vector pQE-1 (Qiagen ...
-
bioRxiv - Molecular Biology 2022Quote: ... 5’ phosphorylated by T4 polynucleotide kinase (New England Biolabs), ligated to a blunt-end linear vector (typically also PCR products) ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR products were circularized with T4 kinase (NEB, M0201L) and T4 ligase (NEB ...