Labshake search
Citations for New England Biolabs :
601 - 650 of 2232 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: All plasmid protection assays were done in Escherichia coli (strain: NEB Turbo). As described previously12 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Separately seeded cells were transfected with pCMV-GLuc2 plasmids (1 μg, NEB) and GLuc activity was measured using the same kit ...
-
bioRxiv - Biochemistry 2021Quote: ... Plasmid was transformed into Escherichia coli expression strain BL2lλDE3 (New England Biolabs) for expression ...
-
bioRxiv - Genetics 2019Quote: ... The pX459 plasmid was digested with High Fidelity BbsI-HF (NEB #R3539) at 37°C for 10 min followed by gel purification using NucleoSpin Gel and PCR Clean-up Midi kit (#740986.20 ...
-
bioRxiv - Developmental Biology 2019Quote: ... the plasmid was linearized using either XbaI and Kpn1 restriction enzymes (NEB), and SP6 and T7 polymerases (NEB ...
-
bioRxiv - Biochemistry 2019Quote: ... after digestion of the template plasmid with 20 U Dpn I (NEB) for 1 h at 37°C ...
-
bioRxiv - Genetics 2020Quote: Circular plasmids was removed by digestion with DpnI (NEB; cat. no. R0176) at 37°C for 30min ...
-
bioRxiv - Biochemistry 2021Quote: ... 16.5 µg pNIC28-Bsa4 plasmid was digested with 60 U BsaI (NEB) in 100 µL following the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2021Quote: ... 7 μg pNZdmsC3GH plasmid was digested with 40 U of SfiI (NEB), separated on a 1% agarose gel and ...
-
bioRxiv - Biochemistry 2021Quote: Plasmid templates for all EM analyses were linearized with Msc I (NEB).
-
bioRxiv - Physiology 2021Quote: ... PCR fragments and plasmid backbones were assembled using Gibson assembly (NEB – E2611). For Gateway cloning ...
-
bioRxiv - Biophysics 2021Quote: ... The cosmid-I95 plasmid was amplified in Neb5 (New England Biolabs, C2987H) and the DNA was purified using a Qiafilter plasmid midi kit (Qiagen ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Linear transformation cassettes were excised from plasmids with EcoRV (New England Biolabs) prior to transformation.
-
bioRxiv - Genetics 2021Quote: ... All plasmids were assembled with NEBuilder HiFi DNA Assembly Cloning Kit (NEB). See Figure 1A.
-
bioRxiv - Cell Biology 2020Quote: All plasmids were constructed using NEBuilder HiFi DNA assembly master mix (NEB) with PCR products ...
-
bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was prepared by a Monarch® mini-preparation kit (NEB). The inserts were sequenced by two primers (forward primer S1273 from the C-terminal coding region of MBP ...
-
bioRxiv - Molecular Biology 2021Quote: Plasmids were methylated using SssI (New England Biolabs, Frankfurt am Main, Germany) as described previously (36) ...
-
bioRxiv - Microbiology 2020Quote: ... The inserts were ligated into the plasmid using the Quick Ligase (NEB) and the reaction was used to transform DH5α’s ...
-
bioRxiv - Genetics 2020Quote: ... Purified plasmid DNA was digested with DraI (New England BioLabs, Beverly, MA) and used for in vitro transcription reaction to generate gRNA ...
-
bioRxiv - Microbiology 2021Quote: ... Plasmid prib(+)XpA was digested by NruI and SnaBI (New England Biolabs) and ligated to produce prib(+)XpA lacking the poliovirus capsid-encoding region from 1175 to 2956 (prib(+)XpA-Δ1175-2956).
-
Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... plasmids were transformed into SHuffle T7 Express LysY bacteria (NEB Cat# C3030J) and induced with 0.4 mM of IPTG at 16°C for 24h ...
-
bioRxiv - Cancer Biology 2022Quote: ... the target CAR plasmid backbone was linearized with BamHI-HF (NEB, R3136T) and cleaned up using Zymo Research DNA purification kit (Zymo Research ...
-
bioRxiv - Biochemistry 2022Quote: ... Standard plasmid construction using restriction enzymes BspHI and XhoI (New England Biolabs) was used for UMAG_01820 ...
-
bioRxiv - Biophysics 2022Quote: ... and Pat1 were cloned into a modified pMAL plasmid (New England Biolabs) using standard methods and NdeI and BamHI restriction sites ...
-
bioRxiv - Cell Biology 2022Quote: ... All PTet plasmids were generated using Gibson reactions (NEB HiFi Assembly Mix) from PCR products ...
-
bioRxiv - Cell Biology 2022Quote: ... plasmid pMO014 was linearized with BglII (Cat. No. R0144, New England BioLabs) and transformed for integration into the genomic trp1 locus using a LiOAc-based protocol(14) ...
-
bioRxiv - Genomics 2022Quote: ... Purified plasmids were electroporated to ElectroMAX Stbl4 Competent Cells (New England Biolabs) and amplified.
-
bioRxiv - Plant Biology 2022Quote: ... plasmids were linearized with the NheI restriction enzyme (NEB, Ipswich, MA, USA) to transcribe complimentary RNA (cRNA ...
-
bioRxiv - Molecular Biology 2022Quote: ... Mutations were introduced in plasmids with Q5® Site-directed mutagenesis (NEB). All the constructs were validated by DNA sequencing (Eurofins Genomics) ...
-
bioRxiv - Microbiology 2022Quote: ... plasmid DNA was digested with restriction enzyme KpnI following manufacturer’s instructions (NEB). After two hours at 37°C the ultrapure DNA underwent phenol chloroform extraction and ethanol precipitation at -80°C as described previously for ultrapure DNA purification ...
-
bioRxiv - Synthetic Biology 2022Quote: ... All enzymes for plasmid construction were obtained from New England Biolabs (NEB). Constructs were chemically transformed into E ...
-
bioRxiv - Microbiology 2023Quote: ... All other plasmids were constructed using a HiFi DNA assembly kit (NEB). All plasmids were confirmed by Sanger sequencing (Genewiz) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Secondary to plasmid transformation of competent bacteria (New England Biolabs, Ipswich, USA), selection of clones ...
-
bioRxiv - Molecular Biology 2022Quote: ... and purified with the Monarch Plasmid Miniprep Kit (T1010, New England Biolabs). Plasmids were digested with restriction enzymes to generate linearized templates for in vitro transcription ...
-
bioRxiv - Microbiology 2022Quote: ... A VSV-G plasmid (16) was digested with KpnI and HpaI (NEB), removing a C-terminal part of G ...
-
bioRxiv - Molecular Biology 2022Quote: ... by combining the original plasmid digested with HindIII (New England Biolabs, #R3104S) and NotI (New England Biolabs ...
-
bioRxiv - Genomics 2022Quote: 100-ng pTXB1 plasmids were firstly linearized by 10U XbaI (NEB, R0145S) at 37°C for 1h and purified by Zymo columns ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Plasmid assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB), followed by transformation into NEB 5-alpha competent E ...
-
bioRxiv - Molecular Biology 2023Quote: ... The plasmids were constructed by NEBuilder HiFi DNA assembly (New England Biolabs) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2022Quote: ... This plasmid was digested with high fidelity (HF) PstI and XhoI (NEB) before insertion of the amplified GPR1 using NEBuilder® HiFI DNA assembly Cloning kit ...
-
bioRxiv - Microbiology 2022Quote: ... Therefore the CIp10 plasmid was digested with HF PstI and XhoI (NEB) before insertion of the amplified Gpr1 and the mTurquoise2 ...
-
bioRxiv - Genetics 2023Quote: ... Linear pUC-GW plasmids and purified SEED cassettes were assembled by NEB assembly following commercial protocols for 1h at 50°C ...
-
bioRxiv - Biochemistry 2023Quote: ... and the resultant plasmids were transformed in E.Coli BL21-DE3 cells (NEB).
-
bioRxiv - Genetics 2023Quote: Plasmid px458 was digested with restriction endonuclease BbsI (New England Biolabs, R0539S), by addition of 1μl enzyme to 1μg plasmid ...
-
bioRxiv - Cell Biology 2023Quote: ... The ligated plasmid was transformed into Stable competent cells (New England Biolabs), and individual clones were picked and sequenced (Laragen ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 µg of template plasmid was digested using 1µL AgeI (NEB, R3552S) for 15h in Cutsmart Buffer (NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... Backbone plasmids were linearised via PCR followed by treatment with DpnI (NEB) before assembly with fragments using NEBuilder HiFi DNA Assembly (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were assembled via Gibson Assembly (New England BioLabs 2X Master mix) (62 ...
-
bioRxiv - Systems Biology 2023Quote: ... the plasmid backbone was digested and dephosphorylated using BsmBI restriction enzyme (NEB) and Quick CIP (NEB) ...
-
bioRxiv - Cell Biology 2023Quote: ... The obtained yeast plasmid DNA was transformed into competent cells (NEB, C2987H). Yeast strains ...