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Citations for New England Biolabs :
351 - 400 of 9514 citations for RapidClean Protein Removal kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... 0.8pg of 3xNLS-SpCas9 protein and 1mU of I-SceI (NEB) was injected per embryo at early 1-cell stage ...
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bioRxiv - Plant Biology 2022Quote: ... and MBP-HIS-GFP proteins were purified with amylose resin (NEB) and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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bioRxiv - Molecular Biology 2022Quote: ... 2) 7 µL of Protein Deglycosylation Mix II (NEB, cat. P6044S) per slide was added for deglycosylation and incubated overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... for GST fusion proteins or amylose resin (New England Biolabs #E8021S) for MBP fusion proteins for 1 hour at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... then proteins were eluted in 30μL 3x Sample Loading Buffer (NEB) at room temperature for 10 minutes.
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bioRxiv - Cell Biology 2023Quote: ... A mixture containing 600 ng/uL of Cas9 protein (NEB: M0386) and sgRNA complex at a 1:1 ratio was generated with a total volume of 3 uL ...
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bioRxiv - Neuroscience 2023Quote: ... Select samples were treated on-bead with lambda protein phosphatase (NEB) in the manufacturer’s PMP buffer to remove phosphorylation ...
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bioRxiv - Microbiology 2023Quote: ... MBP-fused proteins were purified from supernatants using amylose resin (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and sizes were estimated using Blue Protein Standard (New England Biolabs). N-terminally 6×His-tagged fusions of human and Drosophila SARM1 TIR have an expected molecular weight of 19.2 kDa.
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bioRxiv - Biochemistry 2023Quote: ... MBP fusion proteins were purified on amylose beads (New England Biolabs) in column buffer (20 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.5) and 4000 U lambda protein phosphatase (New England Biolabs) added per 1 mg digested lysate ...
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bioRxiv - Genomics 2023Quote: ... and 200 ng/µl Cas 9 protein (New England BioLabs, M0386T). Injected individuals were screened (see below genotyping procedure ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were detected using anti-MBP antibody (anti-MBP NEB e8032s) and HRP conjugated secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 μl of Lambda protein phosphatase (P0753L, New England BioLabs) were incubated with the proteins at 30°C for 30 minutes.
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bioRxiv - Molecular Biology 2023Quote: ... Protein-containing fractions were pooled and incubated with Chitin Resin (NEB) for 30 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... SNAP-tagged proteins were labelled with Cell-SiR647 (NEB, Frankfurt, Germany). Labeling was done at RT for 1hr ...
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bioRxiv - Neuroscience 2023Quote: ... 5 µL of Colour Prestained Protein Standard (New England Biolabs #P7719S) was included on each gel ...
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bioRxiv - Neuroscience 2024Quote: ... sgRNAs were mixed with Cas9 protein (M0646M NEB, or B25641 invitrogen) and coinjected into one-cell stage embryos using a microinjector (Eppendorf) ...
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bioRxiv - Biochemistry 2024Quote: ... Dephosphorylation reactions were performed with Lambda Protein Phosphatase (Lambda PP) (NEB). 1,000 units of Lambda PP were utilized per µmol of Ycf1 protein in a 500 µL reaction ...
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bioRxiv - Biochemistry 2024Quote: Purified MBP-fusion proteins were treated with TEV-protease (NEB, P8112S) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was expressed using the strain T7express lysY (NEB, C3010I). Expression was induced with 0.4 mM IPTG at OD600 ∼0.6 at 16°C ...
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bioRxiv - Cell Biology 2024Quote: ... were combined with protein kinase buffer (PK buffer, New England Biolabs: 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... each protein construct was transformed in either BL21 DE3 pLysS (NEB) harbouring a truncated OGT version with an intact catalytic core (OGTSW ...
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bioRxiv - Microbiology 2023Quote: ... Monarch kit (NEB) extracted and 0.1 pmol of each DNA fragment was CPER amplified in a 50 µL reaction containing 200 µM of dNTPs ...
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bioRxiv - Plant Biology 2019Quote: ... MBP-tagged proteins were purified using amylose resin (New England Biolabs, #E8021S) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... and proteins were then deglycosylated with PNGaseF (New England Biolabs, Ipswich, MA) for 2 hours at 37C ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Protein-bound RNAs were end-repaired on beads using T4 PNK (NEB) and 3’-end-labeled with azide-dUTP using yeast poly(A ...
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bioRxiv - Cell Biology 2020Quote: ... MBP-TRIM17 proteins were purified on amylose resin (New England BioLabs, #E8021L) and then eluted in a buffer containing 20 mM maltose before dialysis in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... and 800 units of lambda protein phosphatase (λ-PPase; New England Biolabs) and incubated for 20 min at 30°C under slight agitation ...
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bioRxiv - Cell Biology 2020Quote: ... Interacting MBP-AtPIP5K2 protein was detected using an anti-MBP antibody (NEB). Protein input was detected using an anti-GST antibody (GE Healthcare).
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: For analysis of protein N-glycosylation PNGase F (P0704S, New England Biolabs) was used and the assay was performed according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... 1 ul of 20 μM Cas protein (SpyCas9 and SauCas9 from NEB; Nme2Cas9 was purified as previously described (Edraki et al ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein purification was carried out using an amylose resin purification system (NEB) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... 0.75 M NaCl) and 2 μl protein kinase K (NEB, Ipswich, USA) during 2 h at 60 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... S protein were first incubated with either 1000U/mL PNGase F (NEB) or deactivated PNGase F at 37 °C for 14 h ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µl of a broad range protein marker (New England Biolabs, #P7708) was run alongside the samples on each gel to estimate the molecular weight ...
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bioRxiv - Biochemistry 2019Quote: ... unlabeled AdoMet was left out and 6 μg of MBP2* protein (NEB) was added to each reaction to increase molecular crowding.
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bioRxiv - Biochemistry 2019Quote: ... The protein was then passed over a 2 mL amylose column (NEB) and washed with 3 CV of Flag Wash Buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Each Prx protein variant was generated through Q5 mutagenesis (New England Biolabs). The described primers were ordered from Integrated DNA technologies (IDT ...
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bioRxiv - Biochemistry 2020Quote: ... 30 nM dCas9 protein (dCas9 Nuclease, S. pyogenes, M0652, New England Biolabs), 1–4× 104 Beads@oligo ...
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bioRxiv - Biophysics 2021Quote: RecA protein from Escherichia coli was purchased from New England BioLabs (NEB), Cat ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were dephosphorylated using 1μL Lambda phosphatase at 30 °C (NEB; P0753S) for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... MBP-tagged proteins were purified by amylose affinity chromatography (New England Biolabs) following manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... a catalytic subunit of protein kinase A (PKA Cα, New England Biolabs); Ca2+/calmodulin-dependent protein kinase II (CaMKII ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were supplemented with 1X Protein MetalloPhosphatase buffer (New England Biolabs) and 1 mM MnCl2 ...
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bioRxiv - Neuroscience 2022Quote: ... the recombinant proteins were purified by amylose resin (#E8022S, New England Biolabs) according the manual instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and α-N-acetyl-galactosaminidase (New England Biolabs, 10 U/μg protein). Removal of sialic acids was performed using the α2-3,6,8 neuraminidase (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... Each protein was expressed in T7 Express cells (New England BioLabs, MA) grown in Terrific Broth at 37°C following induction with 0.5mM IPTG ...
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bioRxiv - Biochemistry 2022Quote: ... LEGDF-SNAPf protein was labelled with SNAP-Surface649 (Surf649, New England Biolabs). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... coli for protein purification were derivatives of pMal-c5X (New England Biolabs), which carries an AmpR marker and uses an IPTG-inducible tac promoter to expresses recombinant proteins with a factor Xa cleavable maltose-binding protein (MBP ...