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Citations for New England Biolabs :
101 - 150 of 9720 citations for QuantiChrom Protein Creatinine Ratio Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... was mixed with backbone vector at 1:2.5 mass ratio in Golden Gate reaction using BsmBI restriction endonuclease (NEB) and T4 DNA ligase (NEB) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 20 minutes at 80 °C followed by adding annealed inserts to vector DNA (0.020 pmol) at a 3:1 molar ratio with T4 DNA ligase (400 units; M0202S, New England Biolabs), T4 DNA ligase buffer and nuclease free water to a final volume of 20 μL for 30 minutes at room temperature and then 65 °C for 10 minutes.
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bioRxiv - Microbiology 2022Quote: ... and the seven fragments were ligated at equal molar ratios with 10,000 units of T4 ligase (New England Biolabs) in 100 µL at 16°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Oligos were annealed and phosphorylated using equimolar ratios of oligo pairs with 1X T4 Ligation Buffer (New England Biolabs) and T4 PNK (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... doner RNA and the complementary splint DNA strand were annealed at a molar ratio of 1: 2: 1.5 in T4 DNA ligase buffer (NEB) by incubation for 3 min at 65°C ...
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bioRxiv - Cell Biology 2022Quote: ... the fragment and vector were mixed in a 5:1 ratio and ligated with T4 DNA Ligase (NEB, M0202L) overnight at 16°C ...
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bioRxiv - Biochemistry 2023Quote: The library was ligated to the barcode oligonucleotide at a 7:1 oligo:library ratio overnight at 16 °C with T4 DNA ligase (New England Biolabs). A no-insert negative control was also ligated overnight using identical conditions ...
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bioRxiv - Immunology 2023Quote: ... We pooled the second set of eBlocks at equal molar ratio and used Esp3I Type IIs restriction enzyme (NEB), NEBridge Ligase Master Mix ...
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bioRxiv - Microbiology 2023Quote: ... and the seven fragments were ligated at equal molar ratios with 10,000 units of T4 ligase (New England Biolabs) in 100 μL at 16°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... a ratio of 7:1 (oligo:library) was used overnight at 16 °C with T4 DNA ligase (New England Biolabs). The products were purified and eluted in 6 μL water (Zymo Clean and Concentrate) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01 pmol (2:1 molar ratio of insert:backbone) library and 2.5 uL NEBuilder HiFi DNA Assembly Master Mix (NEB E2621) and incubated at 50 °C for 60 minutes ...
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bioRxiv - Biochemistry 2024Quote: The 1D4 purified receptor was mixed with purified jsGiq or hGi1 heterotrimer at a molar ratio of 1:1.25 and supplemented with apyrase (New England Biolabs) (25 mU/mL ...
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bioRxiv - Evolutionary Biology 2022Quote: The TF samples were expressed by using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) and analyzed in duplicate on two different PBM arrays (HK and ME ...
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bioRxiv - Genomics 2022Quote: ... The TF sample was expressed by using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) and analyzed in duplicate on two different PBM arrays (HK and ME ...
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bioRxiv - Microbiology 2024Quote: ... 1.4 μM of each purified mRNA was used in PURExpress in vitro Protein Synthesis Kit (NEB#E6800) in 10 μl reaction in 384 well Small Volume black plate (Grenier 788 096) ...
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bioRxiv - Biophysics 2021Quote: ... Indel frequencies at the SaCas9 target site were assessed via a T7E1 assay with the EnGen Mutation Detection Kit (NEB), using the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Final Illumina libraries were assessed for quality using an Agilent Bioanalyzer DNA High Sensitivity Assay and qPCR quantification was performed using NEBNext Library Quant kit for Illumina (New England Biolabs). Individual libraries were pooled equimolarly ...
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bioRxiv - Genetics 2019Quote: ... in accordance with the manufacturer’s recommendations and were validated using the Agilent High Sensitivity DNA assay on the Agilent Bioanalyzer 2100 system and quantified by NEBNext Library Quant Kit for Illumina (New England Biolabs). After clustering of the index-coded samples ...
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bioRxiv - Microbiology 2021Quote: ... was utilized using SARS-CoV-2 specific primers[43] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs), which became available in assays after September 15 ...
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bioRxiv - Molecular Biology 2022Quote: ... Concentrations were measured by Qubit RNA HS assay kit and 750 ng of Dnase-treated RNA was used for rRNA depletion by NEB Next rRNA depletion kit (Human/Mouse/Rat) ...
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bioRxiv - Plant Biology 2021Quote: ... All other mutations of the constructs used for in vitro and in vivo assays were introduced using a Q5 site-directed mutagenesis kit (NEB). All constructs were verified by DNA sequencing.
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bioRxiv - Biochemistry 2023Quote: A luminescence based translation inhibition assay was performed using an in-vitro translation PURExpress® Δ Ribosome Kit from (NEB) The constituents of the kit were incubated with 25 ng pMSR DNA template ...
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bioRxiv - Genetics 2019Quote: T7 endonuclease assays (M0302L, NEB) were performed following the manufacturer instructions.
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bioRxiv - Developmental Biology 2020Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
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bioRxiv - Genetics 2022Quote: ... using a T7E1 assay (NEB), and compared to the activity of Tet2 sgRNA that has been shown to modify the mouse genome efficiently95 ...
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bioRxiv - Developmental Biology 2024Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
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bioRxiv - Cell Biology 2020Quote: ... Annealed handles were mixed with the purified 21 kb ARS1-DNA at a molar ratio of 15:1 and ligated with T4 DNA Ligase in 1 × T4 ligase buffer (both NEB) at 16 °C overnight ...
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bioRxiv - Genomics 2020Quote: ... The crRNA and tracrRNA with Alt-R modification (Integrated DNA Technologies) were annealed in a 1:1 ratio to form gRNA that was used in the Cas9 (New England Biolabs) digestion of the SMRTbell libraries ...
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bioRxiv - Microbiology 2019Quote: ... the 11.4 kb fragments of GPV- or HIV- GPP were joined with their cognate 304 bp zip coded partial U3 inserts via Gibson Assembly in a molar ratio of 1:5 per reaction using HiFi DNA assembly mix (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and then mixed the plasmids with gBlocks in a 1:3 molar ratio in the presence of NEBbuilder HiFi DNA (New England Biolabs) assembly mix according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... All digested products were column purified and mixed at a 6:1 molar ratio in the presence of T4 DNA ligase (NEB).
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bioRxiv - Biochemistry 2019Quote: ... Quantification of the ratio of MS2-tagged rRNA to WT rRNA was done via semi-quantitative PCR with Q5 DNA polymerase (NEB). Primers MS2_quant_F/R bind outside the MS2 tagged region of 23S cDNA ...
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bioRxiv - Genetics 2020Quote: ... Oligo pairs were mixed at an equimolar ratio in PCR tubes containing T4 Ligation buffer and T4 polynucleotide kinase (NEB) for 5’ phosphorylation of oligos ...
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bioRxiv - Bioengineering 2021Quote: ... Synthesized library inserts 1-16 were mixed in equimolar ratios and extended to the XbaI recognition site on the pET backbone using Q5 PCR (NEB) with the library insert as a template and XbaI extension and common reverse as primers (Supplementary Table 5) ...
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bioRxiv - Biochemistry 2021Quote: ... The fragments were ligated into the pLSV101 vector (1:1 and 3:1 molar ratios) with T4 DNA ligase (New England Biolabs) (10 °C for 30 s and 30 °C for 30 s alternating overnight) ...
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bioRxiv - Biochemistry 2020Quote: ... The insert and vector DNA were combined in a 3:1 insert:vector molar ratio in a volume of 5 μL and added 15 μL of Gibson Assembly Master Mix (New England BioLabs), consisting of T5 Exonuclease ...
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bioRxiv - Microbiology 2021Quote: ... The hygR insert and the inverse PCR product of the pEX18Ap backbone were mixed at a 5:1 ratio and ligated for 30 minutes at 50°C with New England Biolabs (NEB) Gibson Assembly ® Cloning Kit to create pEX18HygB ...
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bioRxiv - Plant Biology 2022Quote: ... The vector and insert DNA fragments were assembled in a ratio of 1:5 using NEBuilder HiFi DNA Assembly (E5520S, New England Biolabs). The gl2L480P ...
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bioRxiv - Immunology 2020Quote: ... and the ratio of viral genome: human gDNA were estimated using qPCR via Luna Universal qPCR Master Mix (New England Biolabs) using designed primers Gag-Fwd ...
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bioRxiv - Molecular Biology 2019Quote: ... Amplified vector and insert (1:3 ratio) were assembled in 20 µl Gibson reaction (61) as per manufacturer’s instructions (NEB, USA) and 2 µl was transformed into chemically competent E ...
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bioRxiv - Biochemistry 2019Quote: ... Washed resins were resuspended in 1 CV of buffer A and mixed with 10xHis-tagged Gi heterotrimer at the ratio of 2 mg Gi per ml resin with the presence of 25 mU/ml apyrase (New England Biolabs). The resuspended resin/Gi mixture was subjected to irradiation with 495-nm long-passed filter for 10-15 minutes to induce activation of rhodopsin and G protein binding ...
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bioRxiv - Genomics 2019Quote: ... Ligation of pJDrcEPP_lib and minP-Luc2 inserts was performed at a 1:3 ratio as before but with T7 DNA ligase (New England Biolabs). Ligation product was cleaned-up and transformed as before ...
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bioRxiv - Microbiology 2019Quote: ... followed by heat inactivation for 10 min at 80°C and overnight ligation at a 1:3 vector-to-insert ratio using T4 DNA Ligase (NEB) at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... 4 μg of the gapped plasmid was annealed to the appropriate 39 nt oligonucleotides (1:100 molar ratio, Supplementary Table S1) and ligated overnight with T4 DNA Ligase (NEB). Restoration of a KpnI restriction site present in the gapped region confirmed incorporation of mismatch oligonucleotides ...
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bioRxiv - Genomics 2020Quote: ... Purified fragments were mixed at 1:3 molar ratio and assembled with NEBuilder HiFi DNA Assembly Mater Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... the annealed primers and purified digested vector were ligated at a 15:1 insert to backbone molar ratio using Instant Sticky-end Ligase Master Mix (M0370S, NEB). A non-targeting control (miR-NTC ...
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bioRxiv - Biophysics 2020Quote: ... backbone and insert were mixed at a 1 to 3 ratio (40 fmol in total) in CutSmart® Buffer (NEB) supplemented with 3mM ATP and 10mM DTT ...
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bioRxiv - Biochemistry 2022Quote: BsmBI-digested vector and insert were ligated in a molar ratio of 1:100 to a total of 1 μg using T4 DNA ligase (NEB) for 2 h at room temperature ...
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bioRxiv - Genetics 2022Quote: ... or three (for cloning 4 sgRNAs) fragments were ligated in an equimolar ratio in a Golden Gate reaction with T4 ligase (New England Biolabs) and the BsmBI isoschizomer Esp3I (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: Verified amplicons were combined with the pMV306H integrative vector backbone at a ratio of 1:1 v/v and allowed to ligate overnight at 4°C with T4 DNA ligase (NEB). Ligation products were subsequently transformed into chemically competent DH5ɑ E.coli ...