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Citations for New England Biolabs :
501 - 550 of 9263 citations for QuantiChrom Hyaluronidase Inhibitor Screening Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... The samples had been measured the concentration by Bradford assay and then treated with enterokinase (NEB Biolabs) overnight ...
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bioRxiv - Bioengineering 2019Quote: ... The samples had been measured the concentration by Bradford assay and then treated with enterokinase (NEB Biolabs) overnight ...
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bioRxiv - Microbiology 2022Quote: ... The SYBR Green assay was performed with the Luna Universal qPCR Master Mix (NEB, Ipswich, MA, USA) according to the manufacturer’s instructions with primers specific for HSV-1 gB (UL27 gene ...
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bioRxiv - Cell Biology 2022Quote: ... For CAK binding assays CDK2 was dephosphorylated by washing beads once in 1x λ-phosphatase buffer (NEB) supplied with 1 mM MnCl2 (NEB ...
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bioRxiv - Microbiology 2022Quote: ... For the noncapped assay the RNA in the reaction was treated with T4 PNK (NEB, Ipswich, MA) to produce a pool of 5’ monophosphate RNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time PCR was performed using SYBR green gene expression assays (New England Biolabs, catalog no. M3003S) on a QuantStudio 6 instrument (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... in a black-bottomed 96-well plate and gene-specific LA qPCR assays were performed as described earlier18,19,41,42 using Long Amp Taq DNA Polymerase (New England BioLabs). Three transcribed (HPRT ...
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bioRxiv - Developmental Biology 2023Quote: ... and a subset of F0 embryos were dissociated and used in T7 endonuclease activity assays (NEB E3321) in order to validate successful CRISPR-Cas9 activity ...
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bioRxiv - Cancer Biology 2024Quote: The dePARylation activity of PARG was measured in the PARP1 Histone H4 Activity Assay (#K611, Tulip BioLabs). Briefly ...
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bioRxiv - Genomics 2021Quote: ... using the Golden Gate assembly kit (NEB® Golden Gate Assembly Kit #E1601). Each guide sequence was cloned with its own U6 promoter and was followed by a sgRNA scaffold ...
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bioRxiv - Microbiology 2023Quote: ... A RNA cleanup kit (Monarch RNA Cleanup Kit T2050, New England Biolabs, France) was used to further clean the RNA samples ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 μg/ml phenylmethylsulfonyl fluoride and protease inhibitor) and resuspended again in 1 ml MNase digestion buffer with 1,250 Units MNase (NEB Biolabs). Chromatin-MNase mix was incubated at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in 250μL Elution Buffer (Wash Buffer supplemented with homemade TEV and Murine RNase Inhibitor (New England Biolabs)) ...
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bioRxiv - Genomics 2020Quote: ... nuclei were resuspended in approximately 500uL of cold nuclear resuspension buffer (Nuclear wash buffer + 0.4U/uL of murine RNAse inhibitor (New England Biolabs)) with gentle trituration then counted on a hemocytometer ...
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bioRxiv - Immunology 2019Quote: ... Single cells were flow-sorted into 96-well plates containing 2 µL of nuclease-free water with 0.2% Triton X-100 and 4 U murine RNase inhibitor (NEB), centrifuged ...
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bioRxiv - Cell Biology 2021Quote: ... incubated at 37℃ for 12 minutes and then washed with 0.1× SSC buffer (Thermo, AM9770) supplemented with 0.05 U/μl RNase inhibitor (NEB, M0314L). RNA released from the permeabilized tissue and captured by the DNB was reverse transcribed overnight at 42℃ using SuperScript II (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... incubated at 37 °C for 10 minutes and then washed with 0.1x SSC buffer (Thermo, AM9770) supplemented with 0.05 U/μL RNase inhibitor (NEB, M0314L) after toluidine blue in tissues was rinsed off with 80% ethanol ...
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bioRxiv - Genetics 2020Quote: 300 intestinal stem and Paneth cells were sorted into 2 μl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB). RNA was reverse transcribed (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... All buffers used in the nuclei isolation and sorting contained 0.4 U/mL RNAse inhibitor murine (New England Biolabs). Cross-linked samples were incubated with 800 μL quenching buffer (1 M Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were then resuspended in Wash Buffer supplemented with TEV protease and Murine RNase Inhibitor (New England Biolabs), and the bait-associated complexes were eluted for 1-1.5 hours ...
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bioRxiv - Synthetic Biology 2022Quote: ... 8 μL of solution A and 6 μL of solution B were mixed with 16 U RNase Inhibitor (NEB), gBlock templates (2 nM each ...
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bioRxiv - Synthetic Biology 2022Quote: ... T7 RNA polymerase (M0251) and murine RNase inhibitor (M0314) were obtained from New England Biolabs (NEB, Ipswich, MA, USA). gBlock Gene Fragments were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mM MgCl2, 1% CHAPS,2.5 mM DTT, 50 µg/mL cycloheximide, 20 U RNase inhibitor murine, New England BioLabs, EDTA-free protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2023Quote: ... in a 10 μL reaction in buffer (50 mM Tris-HCl pH 7.5, 1 mM DTT, 1 U/μL RNase Inhibitor (NEB)) and incubated for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... with 20 nM DNA or 50 nM RNA with 1 unit RNase Inhibitor (New England Biolabs Inc., Massachusetts, USA), 100 nM methyltransferase ...
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bioRxiv - Molecular Biology 2023Quote: ... 7.5 µL DNA was incubated (14 h, 37°C) with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix ...
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bioRxiv - Immunology 2023Quote: ... were single-cell sorted into 5 µl 1% (v/v) Nonidet P40 Substitute, Tris-HCl (20 mM, pH 8.0) containing 5 U murine RNase inhibitor (NEB) using a FACSAriaIII instrument (BD) ...
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bioRxiv - Genomics 2023Quote: ... The reaction was inactivated at 25 °C for 10 min with 1.25 μL of Murine RNAse inhibitor (New England Biolabs).
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bioRxiv - Synthetic Biology 2023Quote: ... The lipid film was rehydrated by vortexing with 21 μL rehydration solution (14 μL PURExpress Solution A, 0.875 μL RNase Inhibitor (NEB), 17 μM IGEPAL (90mM) ...
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Enhancer heterogeneity of lung neuroendocrine tumors reveals sensitivity to FGF signaling inhibitionbioRxiv - Cancer Biology 2023Quote: ... After precipitation RNA was resuspended in appropriate volume of Rnase free water supplemented with RNA inhibitors (New England Biolabs). RNA samples were stored at -80°C until further use ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were set up as per manufacturer’s instructions with the addition of 4 units of Murine RNase Inhibitor (NEB) and 5% DMSO ...
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bioRxiv - Immunology 2023Quote: ... Single cells of two untreated CeD patients were sorted into 96-well plates containing 2 µl of scRNA-seq catch buffer (0.2% vol/vol Triton X-100 [Sigma] in H2O with 2 U/μl RNase inhibitor [New England Biolabs]) per well using a FACSAriaIII instrument (BD) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X Protoscript II buffer (50 mM Tris-HCl pH 8.3, 75 mM KCl, 3 mM MgCl2) 40 U Murine RNase Inhibitor (NEB), 5 mM DTT and pre-incubated for 10 minutes at 42 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and then washed with 0.1x SSC buffer (Thermo, AM9770) supplemented with 0.05 U/ml RNase inhibitor (NEB, M0314L) (NEB, M0314L). RNA released from the permeabilized tissue and captured by the DNB was reverse transcribed overnight at 42°C using SuperScript II (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... incubated at 37°C for 5 minutes, and then washed with 0.1x SSC buffer (Thermo, AM9770) supplemented with 0.05 U/ml RNase inhibitor (NEB, M0314L) (NEB ...
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bioRxiv - Synthetic Biology 2022Quote: ... plasmid (Monarch® PCR mini-prep kit) and gel extraction kits (Monarch® DNA gel extraction kit) were also obtained from NEB and used according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... on ice for 30 min and protein concentration measured by BCA assay (7780, New England Biolabs, Beverly, USA). Results were analyzed using the Compass software (ProteinSimple) ...
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bioRxiv - Microbiology 2021Quote: ... 20 µl of supernatant was mixed with 50 µl BioLux Gaussia Luciferase Assay substrate (New England Biolabs, Germany) and the luciferase activity was determined in a ClarioStar Plus plate reader (BMG Labtech ...
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bioRxiv - Molecular Biology 2022Quote: LAMP assays were optimized with Loxosceles similis DNA using Master Mix reagent (WarmStart® #M1800 – New England BioLabs). For this ...
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bioRxiv - Molecular Biology 2022Quote: ... end-point PCR assays were carried out with Hot Start Taq 2X Master Mix (New England Biolabs, USA) in a 20 μL reaction mixture containing 0.5 units of taq polymerase ...
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bioRxiv - Microbiology 2020Quote: All the templates required for the assay were labelled with [γ-32P] ATP using T4 DNA ligase (NEB) at room temperature for 1 h ...
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bioRxiv - Immunology 2020Quote: ... The nested PCR assays were run using the NEB enzyme Q5® Hot Start High-Fidelity (NEB, M0491) as follows ...
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bioRxiv - Biochemistry 2023Quote: ... RNA capping enzyme assays were performed in 10 μL of 1x RNA capping buffer (New England Biolabs, Inc.) containing 0.5 μM substrate RNA ...
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bioRxiv - Genomics 2022Quote: ... and ribosomal RNAs were depleted using ribosomal RNA depletion kit (rRNA Depletion Kit, NEB). Ribosomal depleted RNA was used to make the RNAseq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid mini-prep kit and PCR DNA clean-up kits were provided by NEB.
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bioRxiv - Developmental Biology 2021Quote: ... using HiScribe Sp6 kit (NEB) instead of T7.
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bioRxiv - Neuroscience 2021Quote: ... rRNA depletion kit (NEB #E6310), and Ampure XP beads (Beckman #A63881) ...
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bioRxiv - Microbiology 2020Quote: ... the PNGase kit from (NEB) was used per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... NEBNext rRNA Depletion kit (NEB) was used to remove bacterial and eukaryotic rRNA from total RNA isolated from pooled samples ...
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bioRxiv - Genetics 2022Quote: ... HiFi Assembly Cloning Kit (NEB) to insert the LYP1 homology sequence into the BamHI/EcoRV digest of Addgene plasmid #35121 to create the final backbone plasmid BFA0190 (Supplementary Figure 10) ...