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Citations for New England Biolabs :
651 - 700 of 1813 citations for Primary Human Arterial Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Plasmids were transformed into NEB5α cells (C2987; NEB), prepared using the QIAprep Miniprep Kit (27104 ...
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bioRxiv - Genomics 2023Quote: ... coli cells (New England Biolabs, Cat. No. C3040H), plated on an LB agar plate containing ampicillin ...
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bioRxiv - Genomics 2023Quote: ... coli cells (New England Biolabs, Cat. No. C3040H) and plated on an LB agar plate containing ampicillin ...
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bioRxiv - Synthetic Biology 2023Quote: ... into 100 µL 10-beta electrocompetent cells (NEB), and then plated on 10 15 cm LBSpect agar plates for 16-20 h (37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... coli ER2566 cells (New England Biolabs T7 Express) harboring the pFCEX1D plasmid (containing wild-type FsC ...
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bioRxiv - Biochemistry 2023Quote: ... Escherichia coli BL21 (DE3) cells (New England BioLabs) were transformed with this expression construct and grown at 37°C in 2×TY medium ...
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bioRxiv - Biochemistry 2023Quote: ... Escherichia coli BL21 (DE3) cells (New England BioLabs) transformed with this expression plasmid were grown at 37°C in 2×TY medium ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmids were transformed into BL21-alpha cells (NEB) and plated overnight at 37 □C on kanamycin LB plates (50 μg/mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... coli High Efficiency cells (New England BioLabs, USA). The cells were grown in luria broth media containing 3% glucose and 100 μg/mL or ampicillin at 30°C until the O.D ...
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bioRxiv - Synthetic Biology 2023Quote: ... Competent Escherichia coli STABLE cells (New England Biolabs) were used as host strains for recovering the recombinant vector ...
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bioRxiv - Molecular Biology 2023Quote: ... coli NEB 10-beta cells (New England Biolabs) and all constructs were sequence verified using plasmidsaurus Oxford Nanopore long read sequencing ...
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bioRxiv - Genetics 2023Quote: ... coli cells (NEB C2987 or Bioline BIO-85027). Bacterial clones were prepped with a midiprep kit ...
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bioRxiv - Cell Biology 2023Quote: ... SNAP-Cell TMR-star (New England BioLabs, S9105S) and HaloTag ligand CF650 (GoryoChemical A308-02 ...
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bioRxiv - Genetics 2023Quote: ... Assemblies were electroporated into DH5β cells (NEB C3020K), and we recovered >1,000,000 colonies.
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bioRxiv - Synthetic Biology 2024Quote: ... Variants were transformed into BL21(DE3) cells (NEB), a single clone was picked and grown to saturation in LB media supplemented with kanamycin (50 µg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... 5µl NEBNext Cell Lysis Buffer (NEB, Massachusetts, U.S.), were added directly into the corresponding microgroove ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were incubated in 2i medium containing 300 nM SNAP-Cell 647-SiR (New England Biolabs, Ipswich, MA, USA, S9102S) for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: The cells were infected with ZIKV and DENV2 and lysed using the Luna Cell Ready Lysis reagent (New England Biolabs, UK). Quantification of viral RNA was performed from cell lysates using the virus specific primers and their levels were normalized to mRNA of GAPDH (Table 1) ...
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bioRxiv - Bioengineering 2023Quote: Samples were resuspended in 5 µl 1X NEBNext Cell Lysis Buffer of the NEBNext Single Cell/Low Input RNA Library Prep Kit (NEB, E6420). After 5 min incubation at RT ...
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bioRxiv - Cell Biology 2023Quote: ... RNA from Luminal mature and progenitor cells was amplified using the “NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification” (NEB). For RNA-Seq 20,0000 to 50,000 sorted basal and luminal cells from the shTrps1 ubiquitous mouse model (Fig S4 ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genetics 2024Quote: ... HMW DNA was also isolated from six PBMC samples (± 1 x 106 cells) using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, #T3050L) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... For SNAP-Cell 647-SiR (New England Biolabs, Inc.) dye labeling ...
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bioRxiv - Genetics 2021Quote: ... After transformation with BL21 (DE3) competent cells (NEB, C25271), overnight cultures were inoculated in LK media and incubated at 37°C until the OD600 approached ∼0.6 ...
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bioRxiv - Cell Biology 2021Quote: ... or SNAP-Cell 647-SiR (New England Biolabs, S9102S) according to manufacturer’s recommendations ...
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bioRxiv - Genomics 2020Quote: ... we transformed it into 10beta electrocom petent cells (NEB), and obtained 1.39 million unique transformants.
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bioRxiv - Molecular Biology 2022Quote: Cell extracts were prepared in RIPA lysis buffer (NEB) supplemented with protease inhibitors (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... coli BL21 DE3 cells (New England Biolabs, Ipswich, MA). Cells were grown in LB media (VWR ...
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bioRxiv - Neuroscience 2021Quote: ... Protein lysates were generated using cell lysis buffer (NEB) and brief sonication on ice ...
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bioRxiv - Molecular Biology 2021Quote: A single colony of BL21DE competent cells (NEB, C2527H), transformed with PltB (or its mutant-PltB S35A ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed twice in 1× NEBuffer3.1 (NEB, B7003S) and resuspended in 342 μl of 1× NEBuffer3.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... with competent BL21 (DE3) and DH5α cells (NEB, USA). pGAD-T7 Rec (Clontech ...
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bioRxiv - Biophysics 2019Quote: ... coli BL21(DE3) cells (New England Biolabs, Ipswich, MA) and expressed and purified as previously described for SP (REF) ...
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bioRxiv - Molecular Biology 2019Quote: ... All restriction enzymes and NEB5α competent cells (NEB C2987H) are from NEB ...
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bioRxiv - Cancer Biology 2019Quote: ... coli BL21(DE3) chemically competent cells (NEB product # C2530H). The following day ...
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bioRxiv - Biochemistry 2020Quote: ... coli NiCo21 (DE3) cells (New England Biolabs, Ipswich, MA) grown in TBmod plus MOPS medium supplemented with 25μg/ml kanamycin and no biotin were used ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-myc (NEB/Cell Signaling, 2276; 1:1500) o/n at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were transformed into NEB competent cells (NEB C2987I), purified with the NucleoBond Xtra Midi Endotoxin Free kit (Clon-tech 740420.50) ...
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bioRxiv - Cell Biology 2020Quote: ... SNAP Cell-SIR647 (New England BioLabs, S9102S, 0.3 µM); PEG 1500 (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with RNaseH (New England Biolabs, M0297S) for 2 hours ...
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bioRxiv - Synthetic Biology 2021Quote: Glycerol stocks of NiCo21(DE3) cells (New England Biolabs) transformed with each GFP variant were streaked onto LB agar plates with added ampicillin (100 μg/ml ...
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bioRxiv - Physiology 2020Quote: ... phospho-AKT (#9271, 1:1000, Cell signalling, NEB, UK), total-AKT (#9272 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were batch bound to amylose resin (NEB) for 2 hours to capture the 2XMBP tagged BRCA2 proteins ...
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bioRxiv - Cell Biology 2022Quote: ... cells were stained with SiR647-SNAP (New England Biolabs) according to the manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2022Quote: ... Transformed cells (New England Biolabs E.coli Turbo Cat.No C2984H) made competent using the protocol provided in [Additional file 1] were placed in the incubator overnight at 37° and colonies were screened for desired mutants using BsaI-HF®v2 (New England Biolabs Cat.No R3733S).
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bioRxiv - Synthetic Biology 2022Quote: ... The plasmid was transformed into T7 express cells (NEB) according to manufacturer’s instructions and expressed in Luria-Bertani broth at 37 °C with shaking at 180 rpm before induction by IPTG at an optical density of 0.6 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were recovered in SOC outgrowth medium (NEB B9020S) for 1 hour at 37°C before selection on kanamycin plates ...
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bioRxiv - Microbiology 2020Quote: ... 1 µM SNAP-Cell TMR Star (New England Biolabs) was added to label the SNAP tag ...
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bioRxiv - Microbiology 2021Quote: ... coli C2987 chemically competent cells (New England Biolabs, Inc). pUC19::PBP7 was used as a template for Pfu-mediated deletion mutagenesis ...