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Citations for New England Biolabs :
251 - 300 of 803 citations for Prestained SDS PAGE Standards Single Color since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: Plasmids were generated by standard molecular biology techniques including HiFi assembly (New England BioLabs). All plasmids were prepared for microinjection using the NucleoBond Xtra Midiprep kit EF (Machery-Nagel ...
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bioRxiv - Biochemistry 2023Quote: ... and K723G).49 His/MBP-BRAF NTs were created with standard Gibson Assembly (NEB) procedure in the pET28-MBP vector from the following primers ...
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bioRxiv - Biochemistry 2023Quote: Cloning was performed using standard molecular biology techniques to assemble Q5 DNA polymerase (NEB) amplified or synthesized (IDT ...
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bioRxiv - Microbiology 2020Quote: ... a single-pot reaction was set up containing T4 ligase (10 000U) and buffer (NEB), SapI (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... Single step PCR was performed using a One-Taq™ PCR reaction kit (NEB, USA) with 30 amplification cycles and 2 ng of original circular-DNA extract as the DNA template ...
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bioRxiv - Molecular Biology 2021Quote: ... The single-stranded DNA templates were amplified using Phusion High-Fidelity DNA Polymerase (M0530S, NEB) and Illumina TruSeq small-RNA sample barcoded primers ...
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bioRxiv - Plant Biology 2019Quote: ... The single mutations were generated using the Q5 Site-directed mutagenesis kit (New England Biolabs). For ZAR1CCH1 ...
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bioRxiv - Cancer Biology 2020Quote: ... were generated using NEBNext Single Cell/Low Input RNA Library Preparation kit (New England Biolabs) and sequenced in a NextSeq500 sequencer SE75bp (Illumina) ...
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bioRxiv - Plant Biology 2021Quote: ... using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, MA, USA), Iso-Seq Express Oligo Kit ...
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bioRxiv - Genomics 2019Quote: ... single-stranded sgRNA oligos were annealed using T4 PNK in T4 ligation buffer (both NEB), and ligated into digested (BsmBI ...
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bioRxiv - Plant Biology 2022Quote: ... using the 96 single-index NEBNext Multiplex Oligos for Illumina (New England Biolabs, cat# E7335S). Paired-end reads were obtained on an Illumina (150 bps ...
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bioRxiv - Microbiology 2022Quote: ... single cutters KpnI and MluI or predicted restriction sites NlaIII and SfaNI (New England Biolabs). For single-cut linearization ...
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bioRxiv - Molecular Biology 2023Quote: Purified RNA was digested and dephosphorylated to single nucleosides using nucleoside digestion mix (NEB, M0649S) at 37 °C for 2 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μM Rad51 and 10 μM (nucleotide) φX174 circular single-stranded DNA (ccsDNA) (N3023L, NEB) were mixed in reaction buffer (25 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Molecular Biology 2022Quote: ... single cells were lysed in 100 nL lysis mix (10 nL 1X Cutsmart buffer (NEB), 10 nL Proteinase K (Ambion) ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified RNA was digested and dephosphorylated to single nucleosides using Nucleoside Digestion Mix (NEB, M0649S) at 37 °C for 1 h ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England Biolabs) was used to generate cDNA from the extraction ...
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bioRxiv - Genomics 2023Quote: ... the single-stranded probes were further digested with 5 units of USER enzyme (NEB M5505L) in 1x DpnII buffer at 37 °C for 3 hours.
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bioRxiv - Genomics 2023Quote: ... DNA was digested into single nucleosides by using the Nucleoside Digestion Mix (New England Biolabs) enzyme cocktail at 37°C for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... 2.5uL from a single native barcode and 25 µL Blunt/TA Ligase Mix (NEB, M0367S) were added ...
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bioRxiv - Genetics 2023Quote: ... A LEU2 single integration vector was used for cloning the fragments by Gibson assembly (NEB). In the strains carrying overexpression transgenes ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... Single point mutant plasmids were constructed using Q5® site-directed mutagenesis kit (NEB, E0554S) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... A single round of PCR was then performed using Q5 high fidelity DNA polymerase (NEB) with exon-specific primers appended with Illumina forward adapter sequence ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA from single embryonic halves was extracted using Monarch Total RNA Miniprep Kit (NEB #T2010S), following manufacturer’s instructions for mammalian whole blood with some modifications ...
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bioRxiv - Microbiology 2024Quote: ... Deletions of single genes in plasmid pGM255 were performed using the KLD enzyme mix (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... 1% SDS and 1.6 U/µl of proteinase K (New England Biolabs # P8107S) to reverse the crosslinks ...
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bioRxiv - Genetics 2021Quote: ... Then each of the 10 constructs were transformed into standard DH5α competent bacteria (#C2987; NEB) grown overnight in in 500 ml of standard Luria Broth(LB ...
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bioRxiv - Genomics 2020Quote: ... For RNA synthesis we used the NEB Standard RNA Synthesis protocol (E2050, New England Biolabs). We then added 1.5x volumes of ethanol to the reaction ...
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bioRxiv - Immunology 2022Quote: ... Realtime PCR is conducted following manufacturer standard protocol (Luna Universal qPCR Master Mix, NEB Inc). Sybr green RT-PCR primers used in this study is listed in Table 1.
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bioRxiv - Microbiology 2019Quote: ... Inserted DNA fragments were prepared using standard PCR protocols using Phusion polymerase (New England Biolabs). The pMCSG53 vector was linearized by cleavage of the cloning site with SspI ...
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bioRxiv - Synthetic Biology 2020Quote: ... which were then assembled using a standard Golden Gate assembly method (New England Biolabs, E1601S) to create a full-length linear insert ...
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bioRxiv - Immunology 2019Quote: ... was determined by Agilent 2100 Bioanalyzer following the standard protocol for NEBNext Ultra II Stranded mRNA (New England Biolabs). Sequencing was performed on the Illumina NextSeq 500 with paired end 42bp × 42bp reads ...
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bioRxiv - Cell Biology 2019Quote: ... standard restriction enzyme cloning was performed using the protocol described by New England Biolabs (NEB). PACRGΔ1-69 was amplified from the GST-PACRGFL plasmid with PCR primers introduced BamHI (N-terminal ...
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bioRxiv - Genetics 2021Quote: ... Each reaction consisted of 2.5 µL 10X Standard Taq Reaction Buffer (NEB, Ipswich, MA, USA), 0.5 µL 10 mM dNTPs (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... All constructs were generated by standard cloning or by Gibson Assembly (NEBuilder HiFi Assembly, NEB) using XL10-Gold bacteria (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg standard glycoprotein was mixed in 1 μL of 10× glycoprotein denaturing buffer (NEB) (final conc ...
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bioRxiv - Bioengineering 2021Quote: ... EP-PCR was performed using Taq polymerase and the standard Taq buffer (New England Biolabs) with imbalanced dNTP (0.2 mM dATP ...
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bioRxiv - Cell Biology 2020Quote: For cloning standard restriction enzyme digests or oligo annealing and ligations using enzymes from NEB was used ...
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bioRxiv - Genomics 2022Quote: ... 30 cycles of endpoint PCR were performed using NEB Standard Taq (New England Biolabs, USA). PCR products (6 μL ...
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bioRxiv - Microbiology 2019Quote: ... Polymerase Chain Reaction (PCR) was conducted following standard conditions outlined by New England Biolabs (NEB) (Ipswich ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli using standard transformation techniques with NEB 5-Alpha chemically competent cells (New England BioLabs), and to C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and Q5 High-Fidelity DNA polymerase in a standard buffer (New England Biolabs, Ipswich, USA). Amplicons were purified on MinElute PCR Purification columns (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesised using the standard first strand synthesis protocol with random hexamers (S1230, NEB).
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bioRxiv - Immunology 2023Quote: ... 12.5 µl of OneTaq Quick-Load 2X Master Mix with Standard Buffer (New England Biolabs), 2 µl of diluted (1:10 ...
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bioRxiv - Plant Biology 2023Quote: Each PCR consisted of 2.5 µL 10X Standard Taq Reaction Buffer (NEB, Ipswich, MA, USA), 0.5 µL 10 mM dNTPs (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... following the standard IVT protocol and purified using Monarch® RNA Cleanup columns (NEB # T2030).
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were constructed using either standard restriction cloning or HiFi DNA assembly (New England Biolabs) and propagated in DH5α E ...
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bioRxiv - Cell Biology 2023Quote: All constructs were generated by standard cloning or by Gibson Assembly (NEBuilder HiFi Assembly, NEB) using XL10-Gold bacteria (Agilent) ...
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bioRxiv - Plant Biology 2023Quote: ... Taq DNA polymerase (1.75 units) with standard Taq buffer (New England Biolabs, Pickering ON, Canada), 0.2 mM dNTPs and 0.2 μM primers (Table 1) ...
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bioRxiv - Developmental Biology 2021Quote: cDNA was synthesized using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (E6421, NEB, USA) following PacBio Iso-Seq template preparation guidelines ...